UniProtKB - P22599 (A1AT2_MOUSE)
Protein
Alpha-1-antitrypsin 1-2
Gene
Serpina1b
Organism
Mus musculus (Mouse)
Status
Functioni
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.2 Publications
Miscellaneous
Murine alpha-1-antitrypsin is represented by a cluster of up to 6 individual Serpina1-related genes. The precise complement of Serpina1-related genes present varies according to the strain of the animal.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 377 – 378 | Reactive bondBy similarity | 2 |
GO - Molecular functioni
- endopeptidase inhibitor activity Source: MGI
- identical protein binding Source: MGI
- protease binding Source: MGI
- serine-type endopeptidase inhibitor activity Source: MGI
GO - Biological processi
- acute-phase response Source: UniProtKB-KW
- in utero embryonic development Source: MGI
- negative regulation of endopeptidase activity Source: GO_Central
- protein N-linked glycosylation Source: MGI
- response to cytokine Source: MGI
- response to peptide hormone Source: MGI
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Biological process | Acute phase |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-1-antitrypsin 1-2Short name: AAT Alternative name(s): Alpha-1 protease inhibitor 2 Alpha-1-antiproteinase Serine protease inhibitor 1-2 Serine protease inhibitor A1b Short name: Serpin A1b |
Gene namesi | Name:Serpina1b Synonyms:Aat2, Dom2, Spi1-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:891970, Serpina1b |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | By similarityAdd BLAST | 24 | |
ChainiPRO_0000032389 | 25 – 413 | Alpha-1-antitrypsin 1-2Add BLAST | 389 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 64 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 101 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 265 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
CPTACi | non-CPTAC-3556 |
EPDi | P22599 |
jPOSTi | P22599 |
MaxQBi | P22599 |
PaxDbi | P22599 |
PeptideAtlasi | P22599 |
PRIDEi | P22599 |
PTM databases
GlyConnecti | 799, 2 N-Linked glycans (2 sites) |
GlyGeni | P22599, 3 sites, 2 N-linked glycans (2 sites) |
iPTMneti | P22599 |
PhosphoSitePlusi | P22599 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000071178, Expressed in left lobe of liver and 131 other tissues |
ExpressionAtlasi | P22599, baseline and differential |
Genevisiblei | P22599, MM |
Interactioni
GO - Molecular functioni
- identical protein binding Source: MGI
- protease binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203428, 14 interactors |
IntActi | P22599, 2 interactors |
MINTi | P22599 |
STRINGi | 10090.ENSMUSP00000093101 |
Miscellaneous databases
RNActi | P22599, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 368 – 387 | RCLAdd BLAST | 20 |
Domaini
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina1-related genes may determine target protease specificity.By similarity
Sequence similaritiesi
Belongs to the serpin family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2392, Eukaryota |
GeneTreei | ENSGT00940000154493 |
HOGENOMi | CLU_023330_2_1_1 |
InParanoidi | P22599 |
OMAi | KEINKWA |
OrthoDBi | 1124079at2759 |
PhylomeDBi | P22599 |
TreeFami | TF343201 |
Family and domain databases
Gene3Di | 2.30.39.10, 1 hit 3.30.497.10, 1 hit |
InterProi | View protein in InterPro IPR023795, Serpin_CS IPR023796, Serpin_dom IPR000215, Serpin_fam IPR036186, Serpin_sf IPR042178, Serpin_sf_1 IPR042185, Serpin_sf_2 |
PANTHERi | PTHR11461, PTHR11461, 1 hit |
Pfami | View protein in Pfam PF00079, Serpin, 1 hit |
SMARTi | View protein in SMART SM00093, SERPIN, 1 hit |
SUPFAMi | SSF56574, SSF56574, 1 hit |
PROSITEi | View protein in PROSITE PS00284, SERPIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P22599-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTPSISWGLL LLAGLCCMVP SFLAEDVQET DTSQKDQSPA SHEIATNLGD
60 70 80 90 100
FAISLYRELV HQSNTSNIFF SPVSIATAFA MLSLGSKGDT HTQILEGLQF
110 120 130 140 150
NLTQTSEADI HKSFQHLLQT LNRPDSELQL STGNGLFVNN DLKLVEKFLE
160 170 180 190 200
EAKNHYQAEV FSVNFAESEE AKKVINDFVE KGTQGKIVEA VKELDQDTVF
210 220 230 240 250
ALANYILFKG KWKKPFDPEN TEEAEFHVDK STTVKVPMMM LSGMLDVHHC
260 270 280 290 300
SILSSWVLLM DYAGNASAVF LLPEDGKMQH LEQTLNKELI SKILLNRRRR
310 320 330 340 350
LVQIHIPRLS ISGDYNLKTL MSPLGITRIF NNGADLSGIT EENAPLKLSK
360 370 380 390 400
AVHKAVLTID ETGTEAAAAT VFEAVPMSMP PILRFDHPFL FIIFEEHTQS
410
PIFVGKVVDP THK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WSE0 | A0A087WSE0_MOUSE | Alpha-1-antitrypsin 1-2 | Serpina1b | 36 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 | M → L (PubMed:2303252).Curated | 1 | |
Sequence conflicti | 18 | M → L (PubMed:12408969).Curated | 1 | |
Sequence conflicti | 18 | M → L in AAH22109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 18 | M → L in AAH25445 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 18 | M → L in AAH49255 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 203 | A → G in AAA37132 (PubMed:2303252).Curated | 1 | |
Sequence conflicti | 240 | M → T (PubMed:2303252).Curated | 1 | |
Sequence conflicti | 240 | M → T (PubMed:12408969).Curated | 1 | |
Sequence conflicti | 240 | M → T in AAH22109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 240 | M → T in AAH25445 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 240 | M → T in AAH49255 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 252 | I → T (PubMed:2303252).Curated | 1 | |
Sequence conflicti | 252 | I → T (PubMed:12408969).Curated | 1 | |
Sequence conflicti | 252 | I → T in AAH22109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 252 | I → T in AAH25445 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 252 | I → T in AAH49255 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 263 | A → V in AAM47489 (PubMed:12408969).Curated | 1 | |
Sequence conflicti | 263 | A → V in AAH22109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 263 | A → V in AAH25445 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 263 | A → V in AAH49255 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 286 | N → S in AAM47489 (PubMed:12408969).Curated | 1 | |
Sequence conflicti | 286 | N → S in AAH22109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 286 | N → S in AAH25445 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 286 | N → S in AAH49255 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 298 | R → H in AAM47489 (PubMed:12408969).Curated | 1 | |
Sequence conflicti | 298 | R → H in AAH22109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 298 | R → H in AAH25445 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 298 | R → H in AAH49255 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 314 | D → E in AAA37132 (PubMed:2303252).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25529 mRNA Translation: AAA37132.1 AF481949 Genomic DNA Translation: AAM47489.1 BC022109 mRNA Translation: AAH22109.1 BC025445 mRNA Translation: AAH25445.1 BC049255 mRNA Translation: AAH49255.2 M75716 mRNA Translation: AAC28865.1 |
CCDSi | CCDS26138.1 |
PIRi | I49452 I49471 |
RefSeqi | NP_033270.3, NM_009244.4 |
Genome annotation databases
Ensembli | ENSMUST00000095450; ENSMUSP00000093101; ENSMUSG00000071178 ENSMUST00000164454; ENSMUSP00000127266; ENSMUSG00000071178 ENSMUST00000186166; ENSMUSP00000139941; ENSMUSG00000071178 |
GeneIDi | 20701 |
KEGGi | mmu:20701 |
UCSCi | uc007owe.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25529 mRNA Translation: AAA37132.1 AF481949 Genomic DNA Translation: AAM47489.1 BC022109 mRNA Translation: AAH22109.1 BC025445 mRNA Translation: AAH25445.1 BC049255 mRNA Translation: AAH49255.2 M75716 mRNA Translation: AAC28865.1 |
CCDSi | CCDS26138.1 |
PIRi | I49452 I49471 |
RefSeqi | NP_033270.3, NM_009244.4 |
3D structure databases
SMRi | P22599 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203428, 14 interactors |
IntActi | P22599, 2 interactors |
MINTi | P22599 |
STRINGi | 10090.ENSMUSP00000093101 |
PTM databases
GlyConnecti | 799, 2 N-Linked glycans (2 sites) |
GlyGeni | P22599, 3 sites, 2 N-linked glycans (2 sites) |
iPTMneti | P22599 |
PhosphoSitePlusi | P22599 |
Proteomic databases
CPTACi | non-CPTAC-3556 |
EPDi | P22599 |
jPOSTi | P22599 |
MaxQBi | P22599 |
PaxDbi | P22599 |
PeptideAtlasi | P22599 |
PRIDEi | P22599 |
Genome annotation databases
Ensembli | ENSMUST00000095450; ENSMUSP00000093101; ENSMUSG00000071178 ENSMUST00000164454; ENSMUSP00000127266; ENSMUSG00000071178 ENSMUST00000186166; ENSMUSP00000139941; ENSMUSG00000071178 |
GeneIDi | 20701 |
KEGGi | mmu:20701 |
UCSCi | uc007owe.1, mouse |
Organism-specific databases
CTDi | 20701 |
MGIi | MGI:891970, Serpina1b |
Phylogenomic databases
eggNOGi | KOG2392, Eukaryota |
GeneTreei | ENSGT00940000154493 |
HOGENOMi | CLU_023330_2_1_1 |
InParanoidi | P22599 |
OMAi | KEINKWA |
OrthoDBi | 1124079at2759 |
PhylomeDBi | P22599 |
TreeFami | TF343201 |
Miscellaneous databases
BioGRID-ORCSi | 20701, 2 hits in 13 CRISPR screens |
ChiTaRSi | Serpina1b, mouse |
PROi | PR:P22599 |
RNActi | P22599, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000071178, Expressed in left lobe of liver and 131 other tissues |
ExpressionAtlasi | P22599, baseline and differential |
Genevisiblei | P22599, MM |
Family and domain databases
Gene3Di | 2.30.39.10, 1 hit 3.30.497.10, 1 hit |
InterProi | View protein in InterPro IPR023795, Serpin_CS IPR023796, Serpin_dom IPR000215, Serpin_fam IPR036186, Serpin_sf IPR042178, Serpin_sf_1 IPR042185, Serpin_sf_2 |
PANTHERi | PTHR11461, PTHR11461, 1 hit |
Pfami | View protein in Pfam PF00079, Serpin, 1 hit |
SMARTi | View protein in SMART SM00093, SERPIN, 1 hit |
SUPFAMi | SSF56574, SSF56574, 1 hit |
PROSITEi | View protein in PROSITE PS00284, SERPIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | A1AT2_MOUSE | |
Accessioni | P22599Primary (citable) accession number: P22599 Secondary accession number(s): Q61283, Q80ZH5, Q8VC20 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | June 21, 2005 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families