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Entry version 112 (08 May 2019)
Sequence version 2 (01 Jul 1993)
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Protein

Beta-mannanase/endoglucanase A

Gene

manA

Organism
Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Degradation of hemicelluloses, the second most abundant polysaccharides in nature. Contains two catalytic domains with mannanase and endoglucanase activities.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans. EC:3.2.1.78
  • Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Optimum pH is 6.

Temperature dependencei

Optimum temperature is 80 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei162Proton donorBy similarity1
Active sitei257NucleophileBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosidase, Hydrolase, Multifunctional enzyme
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
CBM3 Carbohydrate-Binding Module Family 3
GH44 Glycoside Hydrolase Family 44
GH5 Glycoside Hydrolase Family 5

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Beta-mannanase/endoglucanase A
Including the following 2 domains:
Mannan endo-1,4-beta-mannosidase A (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase
Endo-1,4-mannanase
Endo-1,4-beta-glucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:manA
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCaldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri44001 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 41Sequence analysisAdd BLAST41
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000000789942 – 1331Beta-mannanase/endoglucanase AAdd BLAST1290

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P22533

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P22533

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini363 – 516CBM3 1PROSITE-ProRule annotationAdd BLAST154
Domaini566 – 719CBM3 2PROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni42 – 325Catalytic (mannanase)Add BLAST284
Regioni781 – 1331Catalytic (endoglucanase)Add BLAST551

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi326 – 361Pro/Ser/Thr-rich (PT box)Add BLAST36
Compositional biasi519 – 564Pro/Ser/Thr-rich (PT box)Add BLAST46
Compositional biasi721 – 780Pro/Ser/Thr-rich (PT box)Add BLAST60

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 44 (cellulase J) family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4106R7G Bacteria
COG2730 LUCA

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.40.1180, 1 hit
2.60.40.710, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR001956 CBM3
IPR036966 CBM3_sf
IPR024745 Glyco_hydro_44
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00942 CBM_3, 2 hits
PF00150 Cellulase, 1 hit
PF12891 Glyco_hydro_44, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01067 CBM_3, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49384 SSF49384, 2 hits
SSF51445 SSF51445, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51172 CBM3, 2 hits
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P22533-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLKTKIRKK WLSVLCTVVF LLNILFIANV TILPKVGAAT SNDGVVKIDT
60 70 80 90 100
STLIGTNHAH CWYRDRLDTA LRGIRSWGMN SVRVVLSNGY RWTKIPASEV
110 120 130 140 150
ANIISLSRSL GFKAIILEVH DTTGYGEDGA ACSLAQAVEY WKEIKSVLDG
160 170 180 190 200
NEDFVIINIG NEPYGNNNYQ NWVNDTKNAI KALRDAGFKH TIMVDAPNWG
210 220 230 240 250
QDWSNTMRDN AQSIMEADPL RNLVFSIHMY GVYNTASKVE EYIKSFVDKG
260 270 280 290 300
LPLVIGEFGH QHTDGDPDEE AIVRYAKQYK IGLFSWSWCG NSSYVGYLDM
310 320 330 340 350
VNNWDPNNPT PWGQWYKTNA IGTSSTPTPT STVTPTPTPT PTPTPTVTAT
360 370 380 390 400
PTPTPTPVST PATSGQIKVL YANKETNSTT NTIRPWLKVV NSGSSSIDLS
410 420 430 440 450
RVTIRYWYTV DGERAQSAIS DWAQIGASNV TFKFVKLSSS VSGADYYLEI
460 470 480 490 500
GFKSGAGQLQ PGKDTGEIQM RFNKDDWSNY NQGNDWSWIQ SMTSYGENEK
510 520 530 540 550
VTAYIDGVLV WGQEPSGATP APAPTATPTP TPTVTPTPTV TPTPTVTATP
560 570 580 590 600
TPTPTPTPTP VSTPATGGQI KVLYANKETN STTNTIRPWL KVVNSGSSSI
610 620 630 640 650
DLSRVTIRYW YTVDGERAQS AISDWAQIGA SNVTFKFVKL SSSVSGADYY
660 670 680 690 700
LEIGFKSGAG QLQPGKDTGE IQIRFNKSDW SNYNQGNDWS WIQSMTSYGE
710 720 730 740 750
NEKVTAYIDG VLVWGQEPSG TTPSPTSTPT VTVTPTPTPT PTPTPTPTVT
760 770 780 790 800
PTPTVTPTPT VTATPTPTPT PIPTVTPLPT ISPSPSVVEI TINTNAGRTQ
810 820 830 840 850
ISPYIYGANQ DIEGVVHSAR RLGGNRLTGY NWENNFSNAG NDWYHSSDDY
860 870 880 890 900
LCWSMGISGE DAKVPAAVVS KFHEYSLKNN AYSAVTLQMA GYVSKDNYGT
910 920 930 940 950
VSENETAPSN RWAEVKFKKD APLSLNPDLN DNFVYMDEFI NYLINKYGMA
960 970 980 990 1000
SSPTGIKGYI LDNEPDLWAS THPRIHPNKV TCKELIEKSV ELAKVIKTLD
1010 1020 1030 1040 1050
PSAEVFGYAS YGFMGYYSLQ DAPDWNQVKG EHRWFISWYL EQMKKASDSF
1060 1070 1080 1090 1100
GKRLLDVLDL HWYPEARGGN IRVCFDGEND TSKEVVIARM QAPRTLWDPT
1110 1120 1130 1140 1150
YKTSVKGQIT AGENSWINQW FSDYLPIIPN VKADIEKYYP GTKLAISEFD
1160 1170 1180 1190 1200
YGGRNHISGG IALADVLGIF GKYGVNFAAR WGDSGSYAAA AYNIYLNYDG
1210 1220 1230 1240 1250
KGSKYGNTNV SANTSDVENM PVYASINGQD DSELHIILIN RNYDQKLQVK
1260 1270 1280 1290 1300
INITSTPKYT KAEIYGFDSN SPEYKKMGNI DNIESNVFTL EVPKFNGVSH
1310 1320 1330
SITLDFNVSI KIIQNEVIKF IRNLVFMRAL V
Length:1,331
Mass (Da):146,893
Last modified:July 1, 1993 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iFFBCA51BB8D8F0E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti338T → P (PubMed:2039230).Curated1
Sequence conflicti340 – 346TPTPTPT → RQHQHRQ (PubMed:2039230).Curated7

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
L01257 Unassigned DNA Translation: AAA71887.1
M36063 Genomic DNA Translation: AAA72861.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A48954

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01257 Unassigned DNA Translation: AAA71887.1
M36063 Genomic DNA Translation: AAA72861.1
PIRiA48954

3D structure databases

SMRiP22533
ModBaseiSearch...

Protein family/group databases

CAZyiCBM3 Carbohydrate-Binding Module Family 3
GH44 Glycoside Hydrolase Family 44
GH5 Glycoside Hydrolase Family 5

Proteomic databases

PRIDEiP22533

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106R7G Bacteria
COG2730 LUCA

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
2.60.40.710, 2 hits
InterProiView protein in InterPro
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR001956 CBM3
IPR036966 CBM3_sf
IPR024745 Glyco_hydro_44
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00942 CBM_3, 2 hits
PF00150 Cellulase, 1 hit
PF12891 Glyco_hydro_44, 1 hit
SMARTiView protein in SMART
SM01067 CBM_3, 2 hits
SUPFAMiSSF49384 SSF49384, 2 hits
SSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS51172 CBM3, 2 hits
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMANB_CALSA
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P22533
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: July 1, 1993
Last modified: May 8, 2019
This is version 112 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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