UniProtKB - P22523 (MUKB_ECOLI)
Protein
Chromosome partition protein MukB
Gene
mukB
Organism
Escherichia coli (strain K12)
Status
Functioni
Plays a central role in chromosome condensation, segregation and cell cycle progression. Functions as a homodimer, which is essential for chromosome partition. Involved in negative DNA supercoiling in vivo, and by this means organizes and compacts chromosomes. May achieve or facilitate chromosome segregation by condensation of DNA from both sides of a centrally located replisome during cell division. Stimulates both DNA relaxation and to a lesser extent decatenation activity of topoisomerase IV.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 41 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: EcoCyc
- DNA binding Source: EcoCyc
- GTP binding Source: EcoCyc
- identical protein binding Source: EcoCyc
GO - Biological processi
- cell division Source: EcoCyc
- chromosome condensation Source: EcoCyc
- chromosome segregation Source: EcoliWiki
- DNA replication Source: UniProtKB-UniRule
- sister chromatid cohesion Source: EcoliWiki
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, Chromosome partition, DNA condensation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10618-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Chromosome partition protein MukBAlternative name(s): Structural maintenance of chromosome-related protein |
Gene namesi | Name:mukB Ordered Locus Names:b0924, JW0907 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- nucleoid 2 Publications Note: Restricted to the nucleoid region, far from the cell poles.
GO - Cellular componenti
- condensin complex Source: EcoCyc
- cytosol Source: EcoCyc
- nucleoid Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 33 | S → F in mukB106; no effect. 1 Publication | 1 | |
Mutagenesisi | 40 | K → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 688 | E → A: Does not rescue a ts-mutant of MukB. 1 Publication | 1 | |
Mutagenesisi | 692 | D → A: Does not rescue a ts-mutant of MukB; less binding of ParC. 1 Publication | 1 | |
Mutagenesisi | 1201 | D → N in mukB33; no effect. 1 Publication | 1 | |
Mutagenesisi | 1381 | V → L: Abolishes DNA-binding, but remains associated with MukE and MukF. 1 Publication | 1 | |
Mutagenesisi | 1403 | L → P: Abolishes association with MukE and MukF, but still binds DNA. 1 Publication | 1 | |
Mutagenesisi | 1404 | F → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 1406 | D → N: No effect. 1 Publication | 1 | |
Mutagenesisi | 1428 | M → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 1429 | Q → R: Abolishes association with MukE and MukF, but still binds DNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068214 | 1 – 1486 | Chromosome partition protein MukBAdd BLAST | 1486 |
Proteomic databases
jPOSTi | P22523 |
PaxDbi | P22523 |
PRIDEi | P22523 |
Interactioni
Subunit structurei
Homodimerization via its hinge domain. Binds to DNA via its C-terminal region.
Interacts, and probably forms a ternary complex, with MukE and MukF via its C-terminal region. The complex formation is stimulated by calcium or magnesium.
Interacts with tubulin-related protein FtsZ.
Identified in a complex with SpoT; IscS and ACP.
Interacts with the ParC subunit of topoisomerase IV.
5 PublicationsBinary interactionsi
P22523
With | #Exp. | IntAct |
---|---|---|
parC [P0AFI2] | 11 | EBI-542943,EBI-878544 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261687, 403 interactors |
ComplexPortali | CPX-1090, MukBEF condensin complex |
DIPi | DIP-10273N |
IntActi | P22523, 58 interactors |
MINTi | P22523 |
STRINGi | 511145.b0924 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P22523 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P22523 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 645 – 804 | Sufficient for ParC bindingAdd BLAST | 160 | |
Regioni | 666 – 783 | Flexible hingeAdd BLAST | 118 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 326 – 418 | Sequence analysisAdd BLAST | 93 | |
Coiled coili | 444 – 480 | Sequence analysisAdd BLAST | 37 | |
Coiled coili | 509 – 603 | Sequence analysisAdd BLAST | 95 | |
Coiled coili | 835 – 923 | Sequence analysisAdd BLAST | 89 | |
Coiled coili | 977 – 1115 | Sequence analysisAdd BLAST | 139 | |
Coiled coili | 1209 – 1266 | Sequence analysisAdd BLAST | 58 |
Domaini
The hinge domain, which separates the large intramolecular coiled coil regions, allows the homodimerization, forming a V-shaped homodimer. The N- and C-terminus together form the head domain.
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | COG3096, Bacteria |
HOGENOMi | CLU_004430_0_0_6 |
InParanoidi | P22523 |
PhylomeDBi | P22523 |
Family and domain databases
Gene3Di | 1.20.1060.30, 1 hit |
HAMAPi | MF_01800, MukB, 1 hit |
InterProi | View protein in InterPro IPR012090, MukB IPR032520, MukB_hinge IPR042501, MukB_hinge_sf IPR007406, MukB_N_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF04310, MukB, 1 hit PF16330, MukB_hinge, 1 hit |
PIRSFi | PIRSF005246, MukB, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
i Sequence
Sequence statusi: Complete.
P22523-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIERGKFRSL TLINWNGFFA RTFDLDELVT TLSGGNGAGK STTMAAFVTA
60 70 80 90 100
LIPDLTLLHF RNTTEAGATS GSRDKGLHGK LKAGVCYSML DTINSRHQRV
110 120 130 140 150
VVGVRLQQVA GRDRKVDIKP FAIQGLPMSV QPTQLVTETL NERQARVLPL
160 170 180 190 200
NELKDKLEAM EGVQFKQFNS ITDYHSLMFD LGIIARRLRS ASDRSKFYRL
210 220 230 240 250
IEASLYGGIS SAITRSLRDY LLPENSGVRK AFQDMEAALR ENRMTLEAIR
260 270 280 290 300
VTQSDRDLFK HLISEATNYV AADYMRHANE RRVHLDKALE FRRELHTSRQ
310 320 330 340 350
QLAAEQYKHV DMARELAEHN GAEGDLEADY QAASDHLNLV QTALRQQEKI
360 370 380 390 400
ERYEADLDEL QIRLEEQNEV VAEAIERQQE NEARAEAAEL EVDELKSQLA
410 420 430 440 450
DYQQALDVQQ TRAIQYNQAI AALNRAKELC HLPDLTADCA AEWLETFQAK
460 470 480 490 500
ELEATEKMLS LEQKMSMAQT AHSQFEQAYQ LVVAINGPLA RNEAWDVARE
510 520 530 540 550
LLREGVDQRH LAEQVQPLRM RLSELEQRLR EQQEAERLLA DFCKRQGKNF
560 570 580 590 600
DIDELEALHQ ELEARIASLS DSVSNAREER MALRQEQEQL QSRIQSLMQR
610 620 630 640 650
APVWLAAQNS LNQLSEQCGE EFTSSQDVTE YLQQLLERER EAIVERDEVG
660 670 680 690 700
ARKNAVDEEI ERLSQPGGSE DQRLNALAER FGGVLLSEIY DDVSLEDAPY
710 720 730 740 750
FSALYGPSRH AIVVPDLSQV TEHLEGLTDC PEDLYLIEGD PQSFDDSVFS
760 770 780 790 800
VDELEKAVVV KIADRQWRYS RFPEVPLFGR AARESRIESL HAEREVLSER
810 820 830 840 850
FATLSFDVQK TQRLHQAFSR FIGSHLAVAF ESDPEAEIRQ LNSRRVELER
860 870 880 890 900
ALSNHENDNQ QQRIQFEQAK EGVTALNRIL PRLNLLADDS LADRVDEIRE
910 920 930 940 950
RLDEAQEAAR FVQQFGNQLA KLEPIVSVLQ SDPEQFEQLK EDYAYSQQMQ
960 970 980 990 1000
RDARQQAFAL TEVVQRRAHF SYSDSAEMLS GNSDLNEKLR ERLEQAEAER
1010 1020 1030 1040 1050
TRAREALRGH AAQLSQYNQV LASLKSSYDT KKELLNDLQR ELQDIGVRAD
1060 1070 1080 1090 1100
SGAEERARIR RDELHAQLSN NRSRRNQLEK ALTFCEAEMD NLTRKLRKLE
1110 1120 1130 1140 1150
RDYFEMREQV VTAKAGWCAV MRMVKDNGVE RRLHRRELAY LSADDLRSMS
1160 1170 1180 1190 1200
DKALGALRLA VADNEHLRDV LRMSEDPKRP ERKIQFFVAV YQHLRERIRQ
1210 1220 1230 1240 1250
DIIRTDDPVE AIEQMEIELS RLTEELTSRE QKLAISSRSV ANIIRKTIQR
1260 1270 1280 1290 1300
EQNRIRMLNQ GLQNVSFGQV NSVRLNVNVR ETHAMLLDVL SEQHEQHQDL
1310 1320 1330 1340 1350
FNSNRLTFSE ALAKLYQRLN PQIDMGQRTP QTIGEELLDY RNYLEMEVEV
1360 1370 1380 1390 1400
NRGSDGWLRA ESGALSTGEA IGTGMSILVM VVQSWEDESR RLRGKDISPC
1410 1420 1430 1440 1450
RLLFLDEAAR LDARSIATLF ELCERLQMQL IIAAPENISP EKGTTYKLVR
1460 1470 1480
KVFQNTEHVH VVGLRGFAPQ LPETLPGTDE APSQAS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 266 | A → R in CAA40776 (PubMed:1989883).Curated | 1 | |
Sequence conflicti | 266 | A → R in BAA06510 (PubMed:7988894).Curated | 1 | |
Sequence conflicti | 318 – 319 | EH → DD in CAA40776 (PubMed:1989883).Curated | 2 | |
Sequence conflicti | 1134 | H → D in CAA40776 (PubMed:1989883).Curated | 1 | |
Sequence conflicti | 1174 – 1175 | SE → VQ in CAA40776 (PubMed:1989883).Curated | 2 | |
Sequence conflicti | 1276 – 1277 | Missing in CAA40776 (PubMed:1989883).Curated | 2 | |
Sequence conflicti | 1276 – 1277 | Missing in BAA06510 (PubMed:7988894).Curated | 2 | |
Sequence conflicti | 1357 – 1380 | WLRAE…SILVM → CCAQSLVHCRPVRRWYRYVD SGV in CAA40776 (PubMed:1989883).CuratedAdd BLAST | 24 | |
Sequence conflicti | 1390 – 1486 | RRLRG…PSQAS → AACAVKISLLAACCSSMKQR DWMLVLSPRCLNCVSVCKCN SSSQRRKISARRKAPPINWC VKSSRIPNTFMSSACEDLRR NSLKRFQELTKRLLRRVKIK QQCRLFFFRKLRFCTKKVAH YGALFFKLLYIRLCKNVRRL YTEDKPDE in CAA40776 (PubMed:1989883).CuratedAdd BLAST | 97 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57550 Genomic DNA Translation: CAA40776.1 D31701 Genomic DNA Translation: BAA06510.1 U00096 Genomic DNA Translation: AAC74010.1 AP009048 Genomic DNA Translation: BAA35670.1 D26440 Genomic DNA Translation: BAA05459.1 |
PIRi | C64832 JH0228 |
RefSeqi | NP_415444.1, NC_000913.3 WP_000572698.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74010; AAC74010; b0924 BAA35670; BAA35670; BAA35670 |
GeneIDi | 945549 |
KEGGi | ecj:JW0907 eco:b0924 |
PATRICi | fig|1411691.4.peg.1352 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57550 Genomic DNA Translation: CAA40776.1 D31701 Genomic DNA Translation: BAA06510.1 U00096 Genomic DNA Translation: AAC74010.1 AP009048 Genomic DNA Translation: BAA35670.1 D26440 Genomic DNA Translation: BAA05459.1 |
PIRi | C64832 JH0228 |
RefSeqi | NP_415444.1, NC_000913.3 WP_000572698.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QHL | X-ray | 2.20 | A | 1-227 | [»] | |
2WMM | X-ray | 2.30 | A/B | 645-804 | [»] | |
3IBP | X-ray | 3.10 | A | 566-863 | [»] | |
4MN4 | X-ray | 2.30 | C/D | 645-804 | [»] | |
6H2X | X-ray | 2.60 | A | 333-526 | [»] | |
A | 893-1053 | [»] | ||||
SMRi | P22523 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261687, 403 interactors |
ComplexPortali | CPX-1090, MukBEF condensin complex |
DIPi | DIP-10273N |
IntActi | P22523, 58 interactors |
MINTi | P22523 |
STRINGi | 511145.b0924 |
Proteomic databases
jPOSTi | P22523 |
PaxDbi | P22523 |
PRIDEi | P22523 |
Genome annotation databases
EnsemblBacteriai | AAC74010; AAC74010; b0924 BAA35670; BAA35670; BAA35670 |
GeneIDi | 945549 |
KEGGi | ecj:JW0907 eco:b0924 |
PATRICi | fig|1411691.4.peg.1352 |
Organism-specific databases
EchoBASEi | EB0613 |
Phylogenomic databases
eggNOGi | COG3096, Bacteria |
HOGENOMi | CLU_004430_0_0_6 |
InParanoidi | P22523 |
PhylomeDBi | P22523 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10618-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P22523 |
PROi | PR:P22523 |
Family and domain databases
Gene3Di | 1.20.1060.30, 1 hit |
HAMAPi | MF_01800, MukB, 1 hit |
InterProi | View protein in InterPro IPR012090, MukB IPR032520, MukB_hinge IPR042501, MukB_hinge_sf IPR007406, MukB_N_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF04310, MukB, 1 hit PF16330, MukB_hinge, 1 hit |
PIRSFi | PIRSF005246, MukB, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUKB_ECOLI | |
Accessioni | P22523Primary (citable) accession number: P22523 Secondary accession number(s): P71227, P77164, Q47398 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | July 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families