UniProtKB - P22509 (FBRL_RAT)
Protein
rRNA 2'-O-methyltransferase fibrillarin
Gene
Fbl
Organism
Rattus norvegicus (Rat)
Status
Functioni
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (By similarity). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).By similarity
Catalytic activityi
- a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- a ribonucleotide in U6 snRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in U6 snRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
GO - Molecular functioni
- ATPase binding Source: RGD
- histone-glutamine methyltransferase activity Source: UniProtKB
- RNA binding Source: GO_Central
- rRNA methyltransferase activity Source: GO_Central
- TFIID-class transcription factor complex binding Source: RGD
GO - Biological processi
- box C/D snoRNA 3'-end processing Source: GO_Central
- histone glutamine methylation Source: UniProtKB
- rRNA methylation Source: UniProtKB
- snoRNA localization Source: RGD
- snoRNA metabolic process Source: RGD
Keywordsi
Molecular function | Methyltransferase, Ribonucleoprotein, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
Reactomei | R-RNO-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: rRNA 2'-O-methyltransferase fibrillarin (EC:2.1.1.-By similarity)Alternative name(s): Histone-glutamine methyltransferase Nucleolar protein 1 U6 snRNA 2'-O-methyltransferase fibrillarinCurated |
Gene namesi | Name:Fbl |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305542, Fbl |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
- nucleoplasm By similarity
Note: Fibrillar region of the nucleolus.
Nucleus
- box C/D snoRNP complex Source: GO_Central
- Cajal body Source: RGD
- dense fibrillar component Source: UniProtKB
- fibrillar center Source: Ensembl
- granular component Source: RGD
- nucleolus Source: RGD
- nucleoplasm Source: RGD
- nucleus Source: RGD
Other locations
- chromosome Source: RGD
- small-subunit processome Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148509 | 1 – 327 | rRNA 2'-O-methyltransferase fibrillarinAdd BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 15 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 21 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 24 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 28 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 31 | Asymmetric dimethylarginine1 Publication | 1 | |
Cross-linki | 90 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 108 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 108 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 115 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 122 | PhosphoserineBy similarity | 1 | |
Modified residuei | 127 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineCombined sources | 1 | |
Modified residuei | 132 | PhosphoserineBy similarity | 1 | |
Cross-linki | 137 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 149 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 164 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 211 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 212 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.By similarity
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).1 Publication
Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P22509 |
PaxDbi | P22509 |
PRIDEi | P22509 |
PTM databases
iPTMneti | P22509 |
PhosphoSitePlusi | P22509 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000019229, Expressed in spleen and 20 other tissues |
Genevisiblei | P22509, RN |
Interactioni
Subunit structurei
Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs.
Component of several ribosomal and nucleolar protein complexes.
Interacts with PRMT5 and UTP20.
Interacts with DDX5 and C1QBP.
Interacts with NOL11.
Interacts with PIH1D1.
Interacts with RRP1B (By similarity).
Interacts with NOLC1 (PubMed:10679015).
By similarity1 PublicationGO - Molecular functioni
- ATPase binding Source: RGD
- TFIID-class transcription factor complex binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 253952, 3 interactors |
IntActi | P22509, 3 interactors |
STRINGi | 10116.ENSRNOP00000026021 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 178 – 179 | S-adenosyl-L-methionine bindingBy similarity | 2 | |
Regioni | 197 – 198 | S-adenosyl-L-methionine bindingBy similarity | 2 | |
Regioni | 222 – 223 | S-adenosyl-L-methionine bindingBy similarity | 2 | |
Regioni | 242 – 245 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1596, Eukaryota |
GeneTreei | ENSGT00550000074792 |
HOGENOMi | CLU_059055_1_0_1 |
InParanoidi | P22509 |
OMAi | NMATHRT |
OrthoDBi | 1411035at2759 |
PhylomeDBi | P22509 |
TreeFami | TF300639 |
Family and domain databases
HAMAPi | MF_00351, RNA_methyltransf_FlpA, 1 hit |
InterProi | View protein in InterPro IPR000692, Fibrillarin IPR020813, Fibrillarin_CS IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01269, Fibrillarin, 1 hit |
PRINTSi | PR00052, FIBRILLARIN |
SMARTi | View protein in SMART SM01206, Fibrillarin, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00566, FIBRILLARIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P22509-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPGFSPRGG GFGGRGGFGD RGGRGGGRGG RGGFGGGRGG FGGGGRGRGG
60 70 80 90 100
GGGGFRGRGG GGGRGGGFQS GGGRGRGGGR GGKRGNQSGK NVMVEPHRHE
110 120 130 140 150
GVFICRGKED ALVTKNLVPG ESVYGEKRVS ISEGDDKIEY RAWNPFRSKL
160 170 180 190 200
AAAILGGVDQ IHIKPGAKVL YLGAASGTTV SHVSDIVGPD GLVYAVEFSH
210 220 230 240 250
RSGRDLINLA KKRTNIIPVI EDARHPHKYR MLIAMVDVIF ADVAQPDQTR
260 270 280 290 300
IVALNAHTFL RNGGHFVISI KANCIDSTAS AEAVFASEVK KMQQENMKPQ
310 320
EQLTLEPYER DHAVVVGVYR PPPKAKN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | K → D AA sequence (PubMed:10679015).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC099198 mRNA Translation: AAH99198.1 |
PIRi | A23887 |
RefSeqi | NP_001020814.1, NM_001025643.1 |
Genome annotation databases
Ensembli | ENSRNOT00000026021; ENSRNOP00000026021; ENSRNOG00000019229 |
GeneIDi | 292747 |
KEGGi | rno:292747 |
UCSCi | RGD:1305542, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC099198 mRNA Translation: AAH99198.1 |
PIRi | A23887 |
RefSeqi | NP_001020814.1, NM_001025643.1 |
3D structure databases
SMRi | P22509 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 253952, 3 interactors |
IntActi | P22509, 3 interactors |
STRINGi | 10116.ENSRNOP00000026021 |
PTM databases
iPTMneti | P22509 |
PhosphoSitePlusi | P22509 |
Proteomic databases
jPOSTi | P22509 |
PaxDbi | P22509 |
PRIDEi | P22509 |
Genome annotation databases
Ensembli | ENSRNOT00000026021; ENSRNOP00000026021; ENSRNOG00000019229 |
GeneIDi | 292747 |
KEGGi | rno:292747 |
UCSCi | RGD:1305542, rat |
Organism-specific databases
CTDi | 2091 |
RGDi | 1305542, Fbl |
Phylogenomic databases
eggNOGi | KOG1596, Eukaryota |
GeneTreei | ENSGT00550000074792 |
HOGENOMi | CLU_059055_1_0_1 |
InParanoidi | P22509 |
OMAi | NMATHRT |
OrthoDBi | 1411035at2759 |
PhylomeDBi | P22509 |
TreeFami | TF300639 |
Enzyme and pathway databases
Reactomei | R-RNO-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
PROi | PR:P22509 |
Gene expression databases
Bgeei | ENSRNOG00000019229, Expressed in spleen and 20 other tissues |
Genevisiblei | P22509, RN |
Family and domain databases
HAMAPi | MF_00351, RNA_methyltransf_FlpA, 1 hit |
InterProi | View protein in InterPro IPR000692, Fibrillarin IPR020813, Fibrillarin_CS IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01269, Fibrillarin, 1 hit |
PRINTSi | PR00052, FIBRILLARIN |
SMARTi | View protein in SMART SM01206, Fibrillarin, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00566, FIBRILLARIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FBRL_RAT | |
Accessioni | P22509Primary (citable) accession number: P22509 Secondary accession number(s): Q4KLH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 30, 2005 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families