UniProtKB - P22415 (USF1_HUMAN)
Protein
Upstream stimulatory factor 1
Gene
USF1
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- identical protein binding Source: IntAct
- protein-containing complex binding Source: ParkinsonsUK-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- protein kinase binding Source: BHF-UCL
- sequence-specific DNA binding Source: BHF-UCL
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- carbon catabolite regulation of transcription Source: BHF-UCL
- cellular response to insulin stimulus Source: BHF-UCL
- glucose homeostasis Source: BHF-UCL
- glucose metabolic process Source: Ensembl
- late viral transcription Source: BHF-UCL
- lipid homeostasis Source: BHF-UCL
- negative regulation of fibrinolysis Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
- regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
- response to hypoxia Source: BHF-UCL
- response to UV Source: BHF-UCL
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P22415 |
Reactomei | R-HSA-9018519, Estrogen-dependent gene expression |
SignaLinki | P22415 |
SIGNORi | P22415 |
Names & Taxonomyi
Protein namesi | Recommended name: Upstream stimulatory factor 1Alternative name(s): Class B basic helix-loop-helix protein 11 Short name: bHLHb11 Major late transcription factor 1 |
Gene namesi | Name:USF1 Synonyms:BHLHB11, USF |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12593, USF1 |
MIMi | 191523, gene |
neXtProti | NX_P22415 |
VEuPathDBi | HostDB:ENSG00000158773.14 |
Subcellular locationi
Nucleus
Nucleus
- nuclear chromatin Source: ParkinsonsUK-UCL
- nucleoplasm Source: HPA
- nucleus Source: LIFEdb
Other locations
- transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Hyperlipidemia, familial combined, 1 (FCHL1)1 Publication
Disease susceptibility is associated with variants affecting the gene represented in this entry.
Disease descriptionA disorder characterized by a variable pattern of elevated levels of serum total cholesterol, triglycerides or both. It is observed in a percentage of individuals with premature coronary heart disease.
Related information in OMIMOrganism-specific databases
DisGeNETi | 7391 |
MalaCardsi | USF1 |
MIMi | 602491, phenotype |
OpenTargetsi | ENSG00000158773 |
PharmGKBi | PA37223 |
Miscellaneous databases
Pharosi | P22415, Tbio |
Genetic variation databases
BioMutai | USF1 |
DMDMi | 137170 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127496 | 1 – 310 | Upstream stimulatory factor 1Add BLAST | 310 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 306 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | P22415 |
jPOSTi | P22415 |
MassIVEi | P22415 |
MaxQBi | P22415 |
PaxDbi | P22415 |
PeptideAtlasi | P22415 |
PRIDEi | P22415 |
ProteomicsDBi | 53989 [P22415-1] 69358 |
PTM databases
iPTMneti | P22415 |
PhosphoSitePlusi | P22415 |
Expressioni
Gene expression databases
Bgeei | ENSG00000158773, Expressed in small intestine Peyer's patch and 123 other tissues |
ExpressionAtlasi | P22415, baseline and differential |
Genevisiblei | P22415, HS |
Organism-specific databases
HPAi | ENSG00000158773, Low tissue specificity |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).
Interacts with varicella-zoster virus IE62 protein.
1 PublicationBinary interactionsi
Hide detailsP22415
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- identical protein binding Source: IntAct
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- protein kinase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 113237, 73 interactors |
ComplexPortali | CPX-3079, USF1-USF2 upstream stimulatory factor complex CPX-3082, USF1 upstream stimulatory factor complex |
CORUMi | P22415 |
DIPi | DIP-654N |
IntActi | P22415, 45 interactors |
MINTi | P22415 |
STRINGi | 9606.ENSP00000357000 |
Miscellaneous databases
RNActi | P22415, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P22415 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P22415 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 199 – 254 | bHLHPROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 271 – 292 | Leucine-zipperAdd BLAST | 22 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000157083 |
InParanoidi | P22415 |
OMAi | PECNIDY |
PhylomeDBi | P22415 |
TreeFami | TF323338 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
IDEALi | IID00121 |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P22415-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV
60 70 80 90 100
FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT
110 120 130 140 150
SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG
160 170 180 190 200
QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR
210 220 230 240 250
RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY
260 270 280 290 300
IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG
310
LEVVIKNDSN
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AQP1 | B1AQP1_HUMAN | Upstream stimulatory factor 1 | USF1 hCG_20846 | 282 | Annotation score: | ||
E9PME6 | E9PME6_HUMAN | Upstream stimulatory factor 1 | USF1 | 217 | Annotation score: | ||
H0YD59 | H0YD59_HUMAN | Upstream stimulatory factor 1 | USF1 | 165 | Annotation score: | ||
E9PJ52 | E9PJ52_HUMAN | Upstream stimulatory factor 1 | USF1 | 48 | Annotation score: | ||
E9PQA2 | E9PQA2_HUMAN | Upstream stimulatory factor 1 | USF1 | 105 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047740 | 1 – 59 | Missing in isoform 2. 1 PublicationAdd BLAST | 59 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55666 mRNA Translation: CAA39201.1 AB017568 Genomic DNA Translation: BAA76541.1 AB098540 mRNA Translation: BAC78384.1 AK314876 mRNA Translation: BAG37391.1 AL832119 mRNA No translation available. AY593992 Genomic DNA Translation: AAS89301.1 AL591806 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW52675.1 BC035505 mRNA Translation: AAH35505.1 |
CCDSi | CCDS1214.1 [P22415-1] |
PIRi | S13525 |
RefSeqi | NP_001263302.1, NM_001276373.1 [P22415-1] NP_009053.1, NM_007122.4 [P22415-1] NP_996888.1, NM_207005.2 [P22415-2] |
Genome annotation databases
Ensembli | ENST00000368020; ENSP00000356999; ENSG00000158773 [P22415-1] ENST00000368021; ENSP00000357000; ENSG00000158773 [P22415-1] |
GeneIDi | 7391 |
KEGGi | hsa:7391 |
UCSCi | uc001fxi.5, human [P22415-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
SeattleSNPs |
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55666 mRNA Translation: CAA39201.1 AB017568 Genomic DNA Translation: BAA76541.1 AB098540 mRNA Translation: BAC78384.1 AK314876 mRNA Translation: BAG37391.1 AL832119 mRNA No translation available. AY593992 Genomic DNA Translation: AAS89301.1 AL591806 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW52675.1 BC035505 mRNA Translation: AAH35505.1 |
CCDSi | CCDS1214.1 [P22415-1] |
PIRi | S13525 |
RefSeqi | NP_001263302.1, NM_001276373.1 [P22415-1] NP_009053.1, NM_007122.4 [P22415-1] NP_996888.1, NM_207005.2 [P22415-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AN4 | X-ray | 2.90 | A/B | 197-260 | [»] | |
SMRi | P22415 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113237, 73 interactors |
ComplexPortali | CPX-3079, USF1-USF2 upstream stimulatory factor complex CPX-3082, USF1 upstream stimulatory factor complex |
CORUMi | P22415 |
DIPi | DIP-654N |
IntActi | P22415, 45 interactors |
MINTi | P22415 |
STRINGi | 9606.ENSP00000357000 |
PTM databases
iPTMneti | P22415 |
PhosphoSitePlusi | P22415 |
Genetic variation databases
BioMutai | USF1 |
DMDMi | 137170 |
Proteomic databases
EPDi | P22415 |
jPOSTi | P22415 |
MassIVEi | P22415 |
MaxQBi | P22415 |
PaxDbi | P22415 |
PeptideAtlasi | P22415 |
PRIDEi | P22415 |
ProteomicsDBi | 53989 [P22415-1] 69358 |
Protocols and materials databases
Antibodypediai | 3773, 351 antibodies |
DNASUi | 7391 |
Genome annotation databases
Ensembli | ENST00000368020; ENSP00000356999; ENSG00000158773 [P22415-1] ENST00000368021; ENSP00000357000; ENSG00000158773 [P22415-1] |
GeneIDi | 7391 |
KEGGi | hsa:7391 |
UCSCi | uc001fxi.5, human [P22415-1] |
Organism-specific databases
CTDi | 7391 |
DisGeNETi | 7391 |
GeneCardsi | USF1 |
HGNCi | HGNC:12593, USF1 |
HPAi | ENSG00000158773, Low tissue specificity |
MalaCardsi | USF1 |
MIMi | 191523, gene 602491, phenotype |
neXtProti | NX_P22415 |
OpenTargetsi | ENSG00000158773 |
PharmGKBi | PA37223 |
VEuPathDBi | HostDB:ENSG00000158773.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000157083 |
InParanoidi | P22415 |
OMAi | PECNIDY |
PhylomeDBi | P22415 |
TreeFami | TF323338 |
Enzyme and pathway databases
PathwayCommonsi | P22415 |
Reactomei | R-HSA-9018519, Estrogen-dependent gene expression |
SignaLinki | P22415 |
SIGNORi | P22415 |
Miscellaneous databases
BioGRID-ORCSi | 7391, 2 hits in 897 CRISPR screens |
ChiTaRSi | USF1, human |
EvolutionaryTracei | P22415 |
GeneWikii | USF1 |
GenomeRNAii | 7391 |
Pharosi | P22415, Tbio |
PROi | PR:P22415 |
RNActi | P22415, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158773, Expressed in small intestine Peyer's patch and 123 other tissues |
ExpressionAtlasi | P22415, baseline and differential |
Genevisiblei | P22415, HS |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
IDEALi | IID00121 |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | USF1_HUMAN | |
Accessioni | P22415Primary (citable) accession number: P22415 Secondary accession number(s): B2RBZ4, Q5SY46, Q7Z5Y1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | February 10, 2021 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references