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Protein

Nucleoside diphosphate kinase B

Gene

NME2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.3 Publications

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP1
Binding sitei60ATP1
Binding sitei88ATP1
Binding sitei94ATP1
Binding sitei105ATP1
Binding sitei115ATP1
Active sitei118Pros-phosphohistidine intermediate1 Publication1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • nucleoside diphosphate kinase activity Source: UniProtKB
  • protein histidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  • cell adhesion Source: HGNC
  • CTP biosynthetic process Source: InterPro
  • GTP biosynthetic process Source: InterPro
  • integrin-mediated signaling pathway Source: UniProtKB
  • negative regulation of apoptotic process Source: HGNC
  • neutrophil degranulation Source: Reactome
  • nucleobase-containing small molecule interconversion Source: Reactome
  • nucleoside triphosphate biosynthetic process Source: UniProtKB
  • positive regulation of epithelial cell proliferation Source: HGNC
  • positive regulation of keratinocyte differentiation Source: HGNC
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • regulation of epidermis development Source: HGNC
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • UTP biosynthetic process Source: InterPro

Keywordsi

Molecular functionActivator, DNA-binding, Kinase, Transferase
Biological processNucleotide metabolism, Transcription, Transcription regulation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04463-MONOMER
ReactomeiR-HSA-499943 Interconversion of nucleotide di- and triphosphates
R-HSA-6798695 Neutrophil degranulation
SignaLinkiP22392
SIGNORiP22392

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase B (EC:2.7.4.6)
Short name:
NDK B
Short name:
NDP kinase B
Alternative name(s):
C-myc purine-binding transcription factor PUF
Histidine protein kinase NDKB (EC:2.7.13.3)
nm23-H2
Gene namesi
Name:NME2
Synonyms:NM23B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000011052.21
HGNCiHGNC:7850 NME2
MIMi156491 gene
neXtProtiNX_P22392

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4831
654364
OpenTargetsiENSG00000011052
ENSG00000243678
PharmGKBiPA162398077

Chemistry databases

ChEMBLiCHEMBL2160
DrugBankiDB00718 Adefovir Dipivoxil
DB04315 Guanosine-5'-Diphosphate
DB00709 Lamivudine
DB00300 Tenofovir

Polymorphism and mutation databases

BioMutaiNME2
DMDMi127983

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001371171 – 152Nucleoside diphosphate kinase BAdd BLAST152

Proteomic databases

EPDiP22392
PaxDbiP22392
PeptideAtlasiP22392
PRIDEiP22392
ProteomicsDBi53986
53987 [P22392-2]
TopDownProteomicsiP22392-1 [P22392-1]
P22392-2 [P22392-2]

2D gel databases

DOSAC-COBS-2DPAGEiP22392
OGPiP22392
UCD-2DPAGEiP22392

PTM databases

iPTMnetiP22392
PhosphoSitePlusiP22392
SwissPalmiP22392

Expressioni

Tissue specificityi

Isoform 1 and isoform 3 are ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000011052
ExpressionAtlasiP22392 baseline and differential
GenevisibleiP22392 HS

Organism-specific databases

HPAiHPA041113

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with CAPN8 (By similarity). Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi110895, 90 interactors
576341, 46 interactors
DIPiDIP-50179N
IntActiP22392, 73 interactors
MINTiP22392
STRINGi9606.ENSP00000376886

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Helixi13 – 17Combined sources5
Helixi21 – 31Combined sources11
Beta strandi34 – 41Combined sources8
Helixi45 – 51Combined sources7
Helixi53 – 55Combined sources3
Helixi61 – 68Combined sources8
Beta strandi73 – 80Combined sources8
Helixi83 – 91Combined sources9
Helixi96 – 98Combined sources3
Helixi104 – 108Combined sources5
Helixi112 – 114Combined sources3
Beta strandi117 – 119Combined sources3
Helixi123 – 133Combined sources11
Helixi136 – 138Combined sources3
Helixi147 – 150Combined sources4

3D structure databases

ProteinModelPortaliP22392
SMRiP22392
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22392

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 66Interaction with AKAP131 PublicationAdd BLAST66

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
COG0105 LUCA
GeneTreeiENSGT00760000119146
HOGENOMiHOG000224564
HOVERGENiHBG000423
InParanoidiP22392
KOiK00940
PhylomeDBiP22392
TreeFamiTF106373

Family and domain databases

Gene3Di3.30.70.141, 1 hit
HAMAPiMF_00451 NDP_kinase, 1 hit
InterProiView protein in InterPro
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR001564 Nucleoside_diP_kinase
IPR023005 Nucleoside_diP_kinase_AS
PfamiView protein in Pfam
PF00334 NDK, 1 hit
PRINTSiPR01243 NUCDPKINASE
SMARTiView protein in SMART
SM00562 NDK, 1 hit
SUPFAMiSSF54919 SSF54919, 1 hit
PROSITEiView protein in PROSITE
PS00469 NDP_KINASES, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P22392-1) [UniParc]FASTAAdd to basket
Also known as: NM23-H2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV

YE
Length:152
Mass (Da):17,298
Last modified:August 1, 1991 - v1
Checksum:i1A5C3F84D7AD272C
GO
Isoform 3 (identifier: P22392-2) [UniParc]FASTAAdd to basket
Also known as: NM23-LV

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MANCERTFIA...DFCIQVGRTM

Note: Based on a naturally occurring readthrough transcript which produces an NME1-NME2 fusion protein.By similarity
Show »
Length:267
Mass (Da):30,137
Checksum:i3AB02272AF24AAC3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0367081M → MANCERTFIAIKPDGVQRGL VGEIIKRFEQKGFRLVGLKF MQASEDLLKEHYVDLKDRPF FAGLVKYMHSGPVVAMVWEG LNVVKTGRVMLGETNPADSK PGTIRGDFCIQVGRTM in isoform 3. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58965 mRNA Translation: CAB37870.1
M36981 mRNA Translation: AAA36369.1
L16785 mRNA Translation: AAA60228.1
DQ109675 mRNA Translation: AAZ82097.1
AC005839 Genomic DNA No translation available.
BC002476 mRNA Translation: AAH02476.1
BC133029 mRNA Translation: AAI33030.1
BC133031 mRNA Translation: AAI33032.1
U29200 Genomic DNA Translation: AAA86745.1
CCDSiCCDS11580.1 [P22392-1]
PIRiA49798
RefSeqiNP_001018146.1, NM_001018136.2 [P22392-2]
NP_001018147.1, NM_001018137.2 [P22392-1]
NP_001018148.1, NM_001018138.1 [P22392-1]
NP_001018149.1, NM_001018139.2 [P22392-1]
NP_002503.1, NM_002512.3 [P22392-1]
UniGeneiHs.463456

Genome annotation databases

EnsembliENST00000393190; ENSP00000376886; ENSG00000243678 [P22392-1]
ENST00000503064; ENSP00000426901; ENSG00000243678 [P22392-1]
ENST00000512737; ENSP00000421064; ENSG00000243678 [P22392-1]
ENST00000513177; ENSP00000425581; ENSG00000243678 [P22392-1]
ENST00000514264; ENSP00000426976; ENSG00000243678 [P22392-1]
GeneIDi4831
654364
KEGGihsa:4831
hsa:654364
UCSCiuc002itj.4 human [P22392-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNDKB_HUMAN
AccessioniPrimary (citable) accession number: P22392
Secondary accession number(s): A8MWA3, Q1WM23, Q6LCT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 18, 2018
This is version 217 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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