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Protein

Hepatocyte nuclear factor 1-alpha

Gene

Hnf1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi199 – 279Homeobox; HNF1-typePROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 1-alpha
Short name:
HNF-1-alpha
Short name:
HNF-1A
Alternative name(s):
Liver-specific transcription factor LF-B1
Short name:
LFB1
Transcription factor 1
Short name:
TCF-1
Gene namesi
Name:Hnf1a
Synonyms:Hnf-1, Hnf-1a, Tcf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:98504 Hnf1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are born at less than half of the expected frequency. Neonates are slightly smaller than normal and do not grow normally. After 5 weeks mice weigh only 50 to 60% as much as their littermates. Mice develop non-insulin-dependent diabetes mellitus (NIDDM) 2 weeks after birth. Mice exhibit elevated levels of blood glucose, combined with reduced blood levels of insulin and insulin-like growth factor I (IGFI). Males and females are sterile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000491161 – 628Hepatocyte nuclear factor 1-alphaAdd BLAST628

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineCombined sources1
Modified residuei74PhosphothreonineCombined sources1
Modified residuei93PhosphoserineBy similarity1
Modified residuei247PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22361
PaxDbiP22361
PeptideAtlasiP22361
PRIDEiP22361

PTM databases

iPTMnetiP22361
PhosphoSitePlusiP22361

Expressioni

Tissue specificityi

Liver.

Gene expression databases

BgeeiENSMUSG00000029556 Expressed in 56 organ(s), highest expression level in metanephros
CleanExiMM_HNF1A
ExpressionAtlasiP22361 baseline and differential
GenevisibleiP22361 MM

Interactioni

Subunit structurei

Binds DNA as a dimer. Interacts with PCBD1. Heterotetramer with PCBD1; formed by a dimer of dimers.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Sirt1Q923E45EBI-5272860,EBI-1802585

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203999, 8 interactors
CORUMiP22361
IntActiP22361, 2 interactors
MINTiP22361
STRINGi10090.ENSMUSP00000031535

Structurei

Secondary structure

1628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP22361
SMRiP22361
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22361

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 31DimerizationAdd BLAST31
Regioni130 – 132Interaction with DNABy similarity3
Regioni143 – 149Interaction with DNABy similarity7
Regioni155 – 158Interaction with DNABy similarity4
Regioni203 – 206Interaction with DNABy similarity4
Regioni263 – 265Interaction with DNABy similarity3
Regioni270 – 273Interaction with DNABy similarity4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi197 – 205Nuclear localization signalSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi71 – 80Asp/Glu-rich (acidic; potential involvement with transcription)10

Sequence similaritiesi

Belongs to the HNF1 homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiENOG410IFA0 Eukaryota
ENOG410ZZZ0 LUCA
GeneTreeiENSGT00920000149039
HOGENOMiHOG000015305
HOVERGENiHBG005980
InParanoidiP22361
KOiK08036
OMAiQSHVAQS
OrthoDBiEOG091G052F
TreeFamiTF320327

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR039066 HNF-1
IPR006899 HNF-1_N
IPR023219 HNF1_dimer_N_dom_sf
IPR006898 HNF1a_C
IPR006897 HNF1b_C
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
PANTHERiPTHR11568 PTHR11568, 1 hit
PfamiView protein in Pfam
PF04814 HNF-1_N, 1 hit
PF04813 HNF-1A_C, 1 hit
PF04812 HNF-1B_C, 1 hit
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF100957 SSF100957, 1 hit
SSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P22361-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVSKLSQLQT ELLAALLESG LSKEALIQAL GEPGPYLMVG EGPLDKGESC
60 70 80 90 100
GGSRGDLTEL PNGLGETRGS EDDTDDDGED FAPPILKELE NLSPEEAAHQ
110 120 130 140 150
KAVVESLLQE DPWRVAKMVK SYLQQHNIPQ REVVDTTGLN QSHLSQHLNK
160 170 180 190 200
GTPMKTQKRA ALYTWYVRKQ REVAQQFTHA GQGGLIEEPT GDELPTKKGR
210 220 230 240 250
RNRFKWGPAS QQILFQAYER QKNPSKEERE TLVEECNRAE CIQRGVSPSQ
260 270 280 290 300
AQGLGSNLVT EVRVYNWFAN RRKEEAFRHK LAMDTYNGPP PGPGPGPALP
310 320 330 340 350
AHSSPGLPTT TLSPSKVHGV RYGQSATSEA AEVPSSSGGP LVTVSAALHQ
360 370 380 390 400
VSPTGLEPSS LLSTEAKLVS ATGGPLPPVS TLTALHSLEQ TSPGLNQQPQ
410 420 430 440 450
NLIMASLPGV MTIGPGEPAS LGPTFTNTGA STLVIGLAST QAQSVPVINS
460 470 480 490 500
MGSSLTTLQP VQFSQPLHPS YQQPLMPPVQ SHVAQSPFMA TMAQLQSPHA
510 520 530 540 550
LYSHKPEVAQ YTHTSLLPQT MLITDTNLST LASLTPTKQV FTSDTEASSE
560 570 580 590 600
PGLHEPPSPA TTIHIPSQDP SNIQHLQPAH RLSTSPTVSS SSLVLYQSSD
610 620
SNGHSHLLPS NHSVIETFIS TQMASSSQ
Length:628
Mass (Da):67,253
Last modified:July 27, 2011 - v2
Checksum:i737920D47BA1F081
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BL72H3BL72_MOUSE
Hepatocyte nuclear factor 1-alpha
Hnf1a
247Annotation score:
H3BKV2H3BKV2_MOUSE
Hepatocyte nuclear factor 1-alpha
Hnf1a
119Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89L → P in AAA37821 (PubMed:2263635).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57966 mRNA Translation: AAA37821.1
AC116500 Genomic DNA No translation available.
CCDSiCCDS19577.1
PIRiA39262
RefSeqiNP_033353.2, NM_009327.3
UniGeneiMm.332607

Genome annotation databases

EnsembliENSMUST00000031535; ENSMUSP00000031535; ENSMUSG00000029556
GeneIDi21405
KEGGimmu:21405
UCSCiuc008zcz.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57966 mRNA Translation: AAA37821.1
AC116500 Genomic DNA No translation available.
CCDSiCCDS19577.1
PIRiA39262
RefSeqiNP_033353.2, NM_009327.3
UniGeneiMm.332607

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F93X-ray2.60E/F/G/H1-32[»]
1G2YX-ray1.00A/B/C/D1-32[»]
1G2ZX-ray1.15A/B1-32[»]
1G39X-ray1.22A/B/C/D1-32[»]
1JB6X-ray1.70A/B1-33[»]
ProteinModelPortaliP22361
SMRiP22361
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203999, 8 interactors
CORUMiP22361
IntActiP22361, 2 interactors
MINTiP22361
STRINGi10090.ENSMUSP00000031535

PTM databases

iPTMnetiP22361
PhosphoSitePlusiP22361

Proteomic databases

MaxQBiP22361
PaxDbiP22361
PeptideAtlasiP22361
PRIDEiP22361

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031535; ENSMUSP00000031535; ENSMUSG00000029556
GeneIDi21405
KEGGimmu:21405
UCSCiuc008zcz.2 mouse

Organism-specific databases

CTDi6927
MGIiMGI:98504 Hnf1a

Phylogenomic databases

eggNOGiENOG410IFA0 Eukaryota
ENOG410ZZZ0 LUCA
GeneTreeiENSGT00920000149039
HOGENOMiHOG000015305
HOVERGENiHBG005980
InParanoidiP22361
KOiK08036
OMAiQSHVAQS
OrthoDBiEOG091G052F
TreeFamiTF320327

Miscellaneous databases

ChiTaRSiTcf7 mouse
EvolutionaryTraceiP22361
PROiPR:P22361
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029556 Expressed in 56 organ(s), highest expression level in metanephros
CleanExiMM_HNF1A
ExpressionAtlasiP22361 baseline and differential
GenevisibleiP22361 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR039066 HNF-1
IPR006899 HNF-1_N
IPR023219 HNF1_dimer_N_dom_sf
IPR006898 HNF1a_C
IPR006897 HNF1b_C
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
PANTHERiPTHR11568 PTHR11568, 1 hit
PfamiView protein in Pfam
PF04814 HNF-1_N, 1 hit
PF04813 HNF-1A_C, 1 hit
PF04812 HNF-1B_C, 1 hit
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF100957 SSF100957, 1 hit
SSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHNF1A_MOUSE
AccessioniPrimary (citable) accession number: P22361
Secondary accession number(s): E9QP86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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