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Protein

Pyruvate kinase

Gene

pkiA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Regulated by phosphoenolpyruvate substrate and allosteric effectors such as fructose 1,6 diphosphate and magnesium.

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gpdA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (enoA)
  5. Pyruvate kinase (pkiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63SubstrateBy similarity1
Metal bindingi65PotassiumBy similarity1
Metal bindingi67PotassiumBy similarity1
Metal bindingi98PotassiumBy similarity1
Metal bindingi99Potassium; via carbonyl oxygenBy similarity1
Sitei254Transition state stabilizerBy similarity1
Metal bindingi256MagnesiumSequence analysis1
Binding sitei279Substrate; via amide nitrogenBy similarity1
Metal bindingi280MagnesiumBy similarity1
Binding sitei280Substrate; via amide nitrogenBy similarity1
Binding sitei312SubstrateBy similarity1
Binding sitei351ADPSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pkiA
Synonyms:pki
ORF Names:AN5210
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome V
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121131 – 526Pyruvate kinaseAdd BLAST526

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP22360

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi162425.CADANIAP00003208

Structurei

3D structure databases

ProteinModelPortaliP22360
SMRiP22360
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000021559
InParanoidiP22360
KOiK00873
OMAiKHEAIEQ
OrthoDBiEOG092C1RXZ

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P22360-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASSSLDHL SNRMKLEWHS KLNTEMVPAK NFRRTSIICT IGPKTNSVEK
60 70 80 90 100
INALRRAGLN VVRMNFSHGS YEYHQSVIDH AREAEKQQAG RPVAIALDTK
110 120 130 140 150
GPEIRTGNTV GDKDIPIKAG HEMNISTDEQ YATASDDQNM YVDYKNITKV
160 170 180 190 200
ISAGKLIYVD DGILSFEVLE VVDDKTLRVR CLNNGNISSR KGVNLPGTDV
210 220 230 240 250
DLPALSEKDI SDLKFGVKNK VDMVFASFIR RGSDIRHIRE VLGEEGREIQ
260 270 280 290 300
IIAKIENQQG VNNFDEILEE TDGVMVARGD LGIEIPAPKV FIAQKMMIAK
310 320 330 340 350
CNIKGKPVIC ATQMLESMTY NPRPTRAEVS DVANAVLDGA DCVMLSGETA
360 370 380 390 400
KGNYPCEAVT MMSETCLLAE VAIPHFNVFD ELRNLAPRPT DTVESIAMAA
410 420 430 440 450
VSASLELNAG AIVVLTTSGN TARMISKYRP VCPIIMVSRN PAATRYSHLY
460 470 480 490 500
RGVWPFYFPE KKPDFNVKIW QEDVDRRLKW GINHGLKLGI INKGDNIVCV
510 520
QGWRGGMGHT NTVRVVPAEE NLGLSE
Length:526
Mass (Da):58,133
Last modified:May 1, 2007 - v2
Checksum:iBCD3A6F35F5D28B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88Q → A in AAA33320 (PubMed:3072099).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36918 Genomic DNA Translation: AAA33320.1
AACD01000089 Genomic DNA Translation: EAA62391.1
BN001305 Genomic DNA Translation: CBF81089.1
PIRiS27364
RefSeqiXP_662814.1, XM_657722.1

Genome annotation databases

EnsemblFungiiCBF81089; CBF81089; ANIA_05210
EAA62391; EAA62391; AN5210.2
GeneIDi2871501
KEGGiani:AN5210.2

Similar proteinsi

Entry informationi

Entry nameiKPYK_EMENI
AccessioniPrimary (citable) accession number: P22360
Secondary accession number(s): C8VF86, Q5B2M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 1, 2007
Last modified: June 20, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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