UniProtKB - P22353 (RM08_YEAST)
54S ribosomal protein L8, mitochondrial
MRPL8
Functioni
Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.
2 PublicationsMiscellaneous
GO - Molecular functioni
- structural constituent of ribosome Source: SGD
GO - Biological processi
- mitochondrial translation Source: SGD
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein |
Names & Taxonomyi
Protein namesi | Recommended name: 54S ribosomal protein L8, mitochondrialAlternative name(s): Mitochondrial large ribosomal subunit protein bL17m1 Publication YmL8 |
Gene namesi | Name:MRPL8 Ordered Locus Names:YJL063C ORF Names:HRD238, J1125 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003599, MRPL8 |
VEuPathDBi | FungiDB:YJL063C |
Subcellular locationi
Mitochondrion
- Mitochondrion 2 Publications
Note: Mitoribosomes are tethered to the mitochondrial inner membrane and spatially aligned with the membrane insertion machinery through two distinct membrane contact sites, formed by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein MBA1.1 Publication
Mitochondrion
- mitochondrial large ribosomal subunit Source: SGD
- mitochondrion Source: SGD
Other locations
- large ribosomal subunit Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000175550 | 2 – 238 | 54S ribosomal protein L8, mitochondrialAdd BLAST | 237 |
Proteomic databases
MaxQBi | P22353 |
PaxDbi | P22353 |
PRIDEi | P22353 |
Interactioni
Subunit structurei
Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 33690, 116 interactors |
ComplexPortali | CPX-1602, 54S mitochondrial large ribosomal subunit |
DIPi | DIP-5672N |
IntActi | P22353, 9 interactors |
MINTi | P22353 |
STRINGi | 4932.YJL063C |
Miscellaneous databases
RNActi | P22353, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3280, Eukaryota |
GeneTreei | ENSGT00390000010698 |
HOGENOMi | CLU_074407_1_3_1 |
InParanoidi | P22353 |
OMAi | RFMMTAK |
Family and domain databases
Gene3Di | 3.90.1030.10, 1 hit |
InterProi | View protein in InterPro IPR040894, Mrpl_C IPR000456, Ribosomal_L17 IPR036373, Ribosomal_L17_sf |
PANTHERi | PTHR14413, PTHR14413, 1 hit |
Pfami | View protein in Pfam PF18502, Mrpl_C, 1 hit PF01196, Ribosomal_L17, 1 hit |
SUPFAMi | SSF64263, SSF64263, 1 hit |
TIGRFAMsi | TIGR00059, L17, 1 hit |
PROSITEi | View protein in PROSITE PS01167, RIBOSOMAL_L17, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTVGIARKLS RDKAHRDALL KNLACQLFQH ESIVSTHAKC KEASRVAERI
60 70 80 90 100
ITWTKRAITT SNSVAQAELK SQIQSQLFLA GDNRKLMKRL FSEIAPRYLE
110 120 130 140 150
RPGGYTRVLR LEPRANDSAP QSVLELVDSP VMSESHTVNR GNLKMWLLVK
160 170 180 190 200
SVINDDANQL PHNPLTLQNL HKVAKFKAEA QLHGEIMLIK QVLLKEMSLP
210 220 230
YDEALENERT QALLKEVYSS SLPKKTKKPS SYVMVPRP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | T → G AA sequence (PubMed:2183197).Curated | 1 | |
Sequence conflicti | 82 | D → G in CAA37834 (PubMed:2183197).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53841 Genomic DNA Translation: CAA37834.1 Z34288 Genomic DNA Translation: CAA84060.1 Z49338 Genomic DNA Translation: CAA89354.1 AY558249 Genomic DNA Translation: AAS56575.1 BK006943 Genomic DNA Translation: DAA08734.1 |
PIRi | S50809 |
RefSeqi | NP_012472.1, NM_001181496.1 |
Genome annotation databases
EnsemblFungii | YJL063C_mRNA; YJL063C; YJL063C |
GeneIDi | 853382 |
KEGGi | sce:YJL063C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53841 Genomic DNA Translation: CAA37834.1 Z34288 Genomic DNA Translation: CAA84060.1 Z49338 Genomic DNA Translation: CAA89354.1 AY558249 Genomic DNA Translation: AAS56575.1 BK006943 Genomic DNA Translation: DAA08734.1 |
PIRi | S50809 |
RefSeqi | NP_012472.1, NM_001181496.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J6B | electron microscopy | 3.20 | L | 1-238 | [»] | |
5MRC | electron microscopy | 3.25 | L | 2-238 | [»] | |
5MRE | electron microscopy | 3.75 | L | 2-238 | [»] | |
5MRF | electron microscopy | 4.97 | L | 2-238 | [»] | |
SMRi | P22353 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33690, 116 interactors |
ComplexPortali | CPX-1602, 54S mitochondrial large ribosomal subunit |
DIPi | DIP-5672N |
IntActi | P22353, 9 interactors |
MINTi | P22353 |
STRINGi | 4932.YJL063C |
Proteomic databases
MaxQBi | P22353 |
PaxDbi | P22353 |
PRIDEi | P22353 |
Genome annotation databases
EnsemblFungii | YJL063C_mRNA; YJL063C; YJL063C |
GeneIDi | 853382 |
KEGGi | sce:YJL063C |
Organism-specific databases
SGDi | S000003599, MRPL8 |
VEuPathDBi | FungiDB:YJL063C |
Phylogenomic databases
eggNOGi | KOG3280, Eukaryota |
GeneTreei | ENSGT00390000010698 |
HOGENOMi | CLU_074407_1_3_1 |
InParanoidi | P22353 |
OMAi | RFMMTAK |
Miscellaneous databases
PROi | PR:P22353 |
RNActi | P22353, protein |
Family and domain databases
Gene3Di | 3.90.1030.10, 1 hit |
InterProi | View protein in InterPro IPR040894, Mrpl_C IPR000456, Ribosomal_L17 IPR036373, Ribosomal_L17_sf |
PANTHERi | PTHR14413, PTHR14413, 1 hit |
Pfami | View protein in Pfam PF18502, Mrpl_C, 1 hit PF01196, Ribosomal_L17, 1 hit |
SUPFAMi | SSF64263, SSF64263, 1 hit |
TIGRFAMsi | TIGR00059, L17, 1 hit |
PROSITEi | View protein in PROSITE PS01167, RIBOSOMAL_L17, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RM08_YEAST | |
Accessioni | P22353Primary (citable) accession number: P22353 Secondary accession number(s): D6VWB8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 179 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families