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Protein

Germination protease

Gene

gpr

Organism
Bacillus megaterium (strain ATCC 12872 / QMB1551)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Initiates the rapid degradation of small, acid-soluble proteins during spore germination.

Catalytic activityi

Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.24.78 656

Protein family/group databases

MEROPSiA25.001

Names & Taxonomyi

Protein namesi
Recommended name:
Germination protease (EC:3.4.24.78)
Alternative name(s):
GPR endopeptidase
Germination proteinase
Spore protease
Gene namesi
Name:gpr
Ordered Locus Names:BMQ_4571
OrganismiBacillus megaterium (strain ATCC 12872 / QMB1551)
Taxonomic identifieri545693 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000935 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000268681 – 151 PublicationAdd BLAST15
ChainiPRO_000002686916 – 370Germination proteaseAdd BLAST355

Post-translational modificationi

Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination.

Keywords - PTMi

Zymogen

Expressioni

Developmental stagei

GPR transcription occurs during sporulation in forespore first by sigma-F and then by sigma-G.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi545693.BMQ_4571

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP22321
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22321

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A25 family.Curated

Phylogenomic databases

HOGENOMiHOG000058956
KOiK06012
OMAiPMGNYIT
OrthoDBiPOG091H0WE2

Family and domain databases

Gene3Di3.40.50.1450, 1 hit
HAMAPiMF_00626 Germination_prot, 1 hit
InterProiView protein in InterPro
IPR023430 Pept_HybD-like_dom_sf
IPR005080 Peptidase_A25
PfamiView protein in Pfam
PF03418 Peptidase_A25, 1 hit
PIRSFiPIRSF019549 Peptidase_A25, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD041835 Peptidase_A25, 1 hit
SUPFAMiSSF53163 SSF53163, 1 hit
TIGRFAMsiTIGR01441 GPR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22321-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKELDLSQY SVRTDLAVEA KDIALENQPK PNNQSEIKGV IVKEKEEQGV
60 70 80 90 100
KISMVEITEE GAEAIGKKKG RYVTLESVGI REQDTEKQEA MEEVFAKELN
110 120 130 140 150
FFIKSLNIPD DASCLVVGLG NLSVTPDALG PKAVDNLLIT RHLFELQPES
160 170 180 190 200
VQDGFRPVSA IVPGVMGMTG IETSDIIFGV VKKVNPDFII AIDALAARSI
210 220 230 240 250
ERVNATIQIS DSGIHPGSGV GNKRKEISYE TLGIPVIAIG IPTVVDAVSI
260 270 280 290 300
TSDTIDFILK HFGREMKEQG KPSKSLLPSG MTFGEKKKLT EDDLPNEEQR
310 320 330 340 350
QTYLGMIGTL PDEEKRRLIH EVLAPLGHNL MVTPKEVDMF IEDMANVVAG
360 370
GLNAALHHEV DQENFGAYTH
Length:370
Mass (Da):40,497
Last modified:August 10, 2010 - v2
Checksum:iF235C361792C37EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89E → EE in AAA22499 (PubMed:1840582).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55262 Genomic DNA Translation: AAA22499.1
CP001983 Genomic DNA Translation: ADE71575.1
PIRiA39198
RefSeqiWP_013059248.1, NC_014019.1

Genome annotation databases

EnsemblBacteriaiADE71575; ADE71575; BMQ_4571
KEGGibmq:BMQ_4571

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55262 Genomic DNA Translation: AAA22499.1
CP001983 Genomic DNA Translation: ADE71575.1
PIRiA39198
RefSeqiWP_013059248.1, NC_014019.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8BX-ray3.00A/B1-370[»]
SMRiP22321
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi545693.BMQ_4571

Protein family/group databases

MEROPSiA25.001

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE71575; ADE71575; BMQ_4571
KEGGibmq:BMQ_4571

Phylogenomic databases

HOGENOMiHOG000058956
KOiK06012
OMAiPMGNYIT
OrthoDBiPOG091H0WE2

Enzyme and pathway databases

BRENDAi3.4.24.78 656

Miscellaneous databases

EvolutionaryTraceiP22321

Family and domain databases

Gene3Di3.40.50.1450, 1 hit
HAMAPiMF_00626 Germination_prot, 1 hit
InterProiView protein in InterPro
IPR023430 Pept_HybD-like_dom_sf
IPR005080 Peptidase_A25
PfamiView protein in Pfam
PF03418 Peptidase_A25, 1 hit
PIRSFiPIRSF019549 Peptidase_A25, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD041835 Peptidase_A25, 1 hit
SUPFAMiSSF53163 SSF53163, 1 hit
TIGRFAMsiTIGR01441 GPR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGPR_BACMQ
AccessioniPrimary (citable) accession number: P22321
Secondary accession number(s): D5DSW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 10, 2010
Last modified: October 10, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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