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UniProtKB - P22317 (HOXF_CUPNH)
Protein
NAD-reducing hydrogenase HoxS subunit alpha
Gene
hoxF
Organism
Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha)
Status
Functioni
Subunits alpha and gamma of HoxS constitute an NADH--oxidoreductase.
Catalytic activityi
- EC:1.12.1.2
Cofactori
Protein has several cofactor binding sites:- FMNCuratedNote: Binds 1 FMN.Curated
- [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 499 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 502 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 505 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 545 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 219 – 228 | NADBy similarity | 10 | |
Nucleotide bindingi | 332 – 379 | FMNBy similarityAdd BLAST | 48 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- FMN binding Source: InterPro
- hydrogen dehydrogenase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD |
Enzyme and pathway databases
BRENDAi | 1.12.1.2, 231 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hoxF Ordered Locus Names:PHG088 |
Encoded oni | Plasmid megaplasmid pHG1 |
Organismi | Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) |
Taxonomic identifieri | 381666 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118564 | 1 – 602 | NAD-reducing hydrogenase HoxS subunit alphaAdd BLAST | 602 |
Interactioni
Subunit structurei
Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunit (hydrogenase dimer).
Protein-protein interaction databases
STRINGi | 381666.PHG088 |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I 51 kDa subunit family.Curated
Phylogenomic databases
eggNOGi | COG1894, Bacteria |
HOGENOMi | CLU_014881_3_2_4 |
OMAi | CDQGPAL |
OrthoDBi | 688908at2 |
Family and domain databases
Gene3Di | 1.10.10.1590, 1 hit 1.20.1440.230, 1 hit 3.40.50.11540, 1 hit |
InterProi | View protein in InterPro IPR001949, NADH-UbQ_OxRdtase_51kDa_CS IPR011538, Nuo51_FMN-bd IPR037225, Nuo51_FMN-bd_sf IPR041921, NuoE_N IPR019575, Nuop51_4Fe4S-bd IPR037207, Nuop51_4Fe4S-bd_sf IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF01512, Complex1_51K, 1 hit PF10589, NADH_4Fe-4S, 1 hit |
SMARTi | View protein in SMART SM00928, NADH_4Fe-4S, 1 hit |
SUPFAMi | SSF140490, SSF140490, 1 hit SSF142019, SSF142019, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00644, COMPLEX1_51K_1, 1 hit PS00645, COMPLEX1_51K_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P22317-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSRITTILE RYRSDRTRLI DILWDVQHEY GHIPDAVLPQ LGAGLKLSPL
60 70 80 90 100
DIRETASFYH FFLDKPSGKY RIYLCNSVIA KINGYQAVRE ALERETGIRF
110 120 130 140 150
GETDPNGMFG LFDTPCIGLS DQEPAMLIDK VVFTRLRPGK ITDIIAQLKQ
160 170 180 190 200
GRSPAEIANP AGLPSQDIAY VDAMVESNVR TKGPVFFRGR TDLRSLLDQC
210 220 230 240 250
LLLKPEQVIE TIVDSRLRGR GGAGFSTGLK WRLCRDAESE QKYVICNADE
260 270 280 290 300
GEPGTFKDRV LLTRAPKKVF VGMVIAAYAI GCRKGIVYLR GEYFYLKDYL
310 320 330 340 350
ERQLQELRED GLLGRAIGGR AGFDFDIRIQ MGAGAYICGD ESALIESCEG
360 370 380 390 400
KRGTPRVKPP FPVQQGYLGK PTSVNNVETF AAVSRIMEEG ADWFRAMGTP
410 420 430 440 450
DSAGTRLLSV AGDCSKPGIY EVEWGVTLNE VLAMVGARDA RAVQISGPSG
460 470 480 490 500
ECVSVAKDGE RKLAYEDLSC NGAFTIFNCK RDLLEIVRDH MQFFVEESCG
510 520 530 540 550
ICVPCRAGNV DLHRKVEWVI AGKACQKDLD DMVSWGALVR RTSRCGLGAT
560 570 580 590 600
SPKPILTTLE KFPEIYQNKL VRHEGPLLPS FDLDTALGGY EKALKDLEEV
TR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | W → G AA sequence (PubMed:2496982).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55230 Genomic DNA Translation: AAC06140.1 AY305378 Genomic DNA Translation: AAP85841.1 |
PIRi | A35385 |
RefSeqi | WP_011154010.1, NZ_CP039289.1 |
Genome annotation databases
EnsemblBacteriai | AAP85841; AAP85841; PHG088 |
GeneIDi | 39976412 |
KEGGi | reh:PHG088 |
PATRICi | fig|381666.6.peg.65 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55230 Genomic DNA Translation: AAC06140.1 AY305378 Genomic DNA Translation: AAP85841.1 |
PIRi | A35385 |
RefSeqi | WP_011154010.1, NZ_CP039289.1 |
3D structure databases
AlphaFoldDBi | P22317 |
SMRi | P22317 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 381666.PHG088 |
Genome annotation databases
EnsemblBacteriai | AAP85841; AAP85841; PHG088 |
GeneIDi | 39976412 |
KEGGi | reh:PHG088 |
PATRICi | fig|381666.6.peg.65 |
Phylogenomic databases
eggNOGi | COG1894, Bacteria |
HOGENOMi | CLU_014881_3_2_4 |
OMAi | CDQGPAL |
OrthoDBi | 688908at2 |
Enzyme and pathway databases
BRENDAi | 1.12.1.2, 231 |
Family and domain databases
Gene3Di | 1.10.10.1590, 1 hit 1.20.1440.230, 1 hit 3.40.50.11540, 1 hit |
InterProi | View protein in InterPro IPR001949, NADH-UbQ_OxRdtase_51kDa_CS IPR011538, Nuo51_FMN-bd IPR037225, Nuo51_FMN-bd_sf IPR041921, NuoE_N IPR019575, Nuop51_4Fe4S-bd IPR037207, Nuop51_4Fe4S-bd_sf IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF01512, Complex1_51K, 1 hit PF10589, NADH_4Fe-4S, 1 hit |
SMARTi | View protein in SMART SM00928, NADH_4Fe-4S, 1 hit |
SUPFAMi | SSF140490, SSF140490, 1 hit SSF142019, SSF142019, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00644, COMPLEX1_51K_1, 1 hit PS00645, COMPLEX1_51K_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOXF_CUPNH | |
Accessioni | P22317Primary (citable) accession number: P22317 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Plasmid, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families