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UniProtKB - P22314 (UBA1_HUMAN)

Entry version 228 (02 Dec 2020)
Sequence version 3 (25 Oct 2002)
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Protein

Ubiquitin-like modifier-activating enzyme 1

Gene

UBA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).3 Publications

Miscellaneous

There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.1 Publication EC:6.2.1.45

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei478ATP; via amide nitrogenBy similarity1
Binding sitei504ATPBy similarity1
Binding sitei515ATPBy similarity1
Binding sitei528ATPBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei632Glycyl thioester intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi576 – 577ATPBy similarity2

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		MetaCyc:HS05465-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.3.2.B5, 2681
6.2.1.B9, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P22314

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation

SIGNOR Signaling Network Open Resource

More...SIGNORi		P22314

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 1 (EC:6.2.1.451 Publication)
Alternative name(s):
Protein A1S9
Ubiquitin-activating enzyme E1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:UBA1
Synonyms:A1S9T, UBE1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome X

Organism-specific databases

Eukaryotic Pathogen and Host Database Resources

More...EuPathDBi		HostDB:ENSG00000130985.16

Human Gene Nomenclature Database

More...HGNCi		HGNC:12469, UBA1

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		314370, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P22314

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Spinal muscular atrophy X-linked 2 (SMAX2)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA lethal infantile form of spinal muscular atrophy, a neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. Clinical features include hypotonia, areflexia, and multiple congenital contractures.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_043501539M → I in SMAX2. 1 PublicationCorresponds to variant dbSNP:rs80356545EnsemblClinVar.1
Natural variantiVAR_043502547S → G in SMAX2. 1 PublicationCorresponds to variant dbSNP:rs80356546EnsemblClinVar.1
Natural variantiVAR_071121557E → V in SMAX2. 1 Publication1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi4S → A: Reduces phosphorylation. 1 Publication1
Mutagenesisi8 – 11KKRR → AAAA: Loss of nuclear localization and a 90-95% decrease in the phosphorylation. 1 Publication4

Keywords - Diseasei

Disease mutation, Neurodegeneration

Organism-specific databases

DisGeNET

More...DisGeNETi		7317

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...GeneReviewsi		UBA1

MalaCards human disease database

More...MalaCardsi		UBA1
MIMi301830, phenotype

Open Targets

More...OpenTargetsi		ENSG00000130985

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		1145, Infantile-onset X-linked spinal muscular atrophy

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA37119

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P22314, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL5924

Drug and drug target database

More...DrugBanki		DB04119, Hexatantalum Dodecabromide
DB04216, Quercetin

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		UBA1

Domain mapping of disease mutations (DMDM)

More...DMDMi		24418865

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001949342 – 1058Ubiquitin-like modifier-activating enzyme 1Add BLAST1057
Isoform 2 (identifier: P22314-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylserineCombined sources1
Modified residuei4Phosphoserine1 Publication1
Modified residuei13PhosphoserineCombined sources1
Modified residuei21PhosphoserineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei46PhosphoserineCombined sources1
Modified residuei55PhosphotyrosineBy similarity1
Modified residuei528N6-succinyllysineBy similarity1
Modified residuei671N6-acetyllysineCombined sources1
Modified residuei800PhosphothreonineCombined sources1
Modified residuei810PhosphoserineCombined sources1
Modified residuei816PhosphoserineBy similarity1
Modified residuei820PhosphoserineCombined sources1
Modified residuei835PhosphoserineCombined sources1
Modified residuei980N6-acetyllysineCombined sources1
Isoform 2 (identifier: P22314-2)
Modified residuei2N-acetylalanineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

The CPTAC Assay portal

More...CPTACi		CPTAC-136
CPTAC-137

Encyclopedia of Proteome Dynamics

More...EPDi		P22314

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P22314

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P22314

MaxQB - The MaxQuant DataBase

More...MaxQBi		P22314

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P22314

PeptideAtlas

More...PeptideAtlasi		P22314

PRoteomics IDEntifications database

More...PRIDEi		P22314

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		53983 [P22314-1]

2D gel databases

REPRODUCTION-2DPAGE

More...REPRODUCTION-2DPAGEi		IPI00645078

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P22314

MetOSite database of methionine sulfoxide sites

More...MetOSitei		P22314

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P22314

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P22314

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Detected in erythrocytes (at protein level). Ubiquitous.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000130985, Expressed in pituitary gland and 256 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P22314, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P22314, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000130985, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer (By similarity).

Interacts with GAN (via BTB domain).

By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		113165, 208 interactors

Database of interacting proteins

More...DIPi		DIP-33686N

Protein interaction database and analysis system

More...IntActi		P22314, 152 interactors

Molecular INTeraction database

More...MINTi		P22314

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000338413

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P22314

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P22314, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11058
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P22314

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati63 – 1991-1Add BLAST137
Repeati459 – 6111-2Add BLAST153

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni63 – 6112 approximate repeatsAdd BLAST549

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi5 – 11Nuclear localization signal7

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The first 11 amino acids are essential for phosphorylation and exclusive nuclear localization.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2012, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000158975

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_002556_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P22314

Identification of Orthologs from Complete Genome Data

More...OMAi		CIVWARL

Database of Orthologous Groups

More...OrthoDBi		91748at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P22314

TreeFam database of animal gene trees

More...TreeFami		TF300586

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.10.2660, 1 hit
2.40.30.180, 1 hit
3.10.290.60, 1 hit
3.50.50.80, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR032420, E1_4HB
IPR032418, E1_FCCH
IPR042302, E1_FCCH_sf
IPR000594, ThiF_NAD_FAD-bd
IPR018965, Ub-activating_enz_E1_C
IPR042449, Ub-E1_IAD_1
IPR038252, UBA_E1_C_sf
IPR019572, UBA_E1_Cys
IPR042063, Ubi_acti_E1_SCCH
IPR035985, Ubiquitin-activating_enz
IPR018075, UBQ-activ_enz_E1
IPR018074, UBQ-activ_enz_E1_CS
IPR033127, UBQ-activ_enz_E1_Cys_AS
IPR000011, UBQ/SUMO-activ_enz_E1-like

Pfam protein domain database

More...Pfami		View protein in Pfam
PF16191, E1_4HB, 1 hit
PF16190, E1_FCCH, 1 hit
PF09358, E1_UFD, 1 hit
PF00899, ThiF, 2 hits
PF10585, UBA_e1_thiolCys, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR01849, UBIQUITINACT

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00985, UBA_e1_C, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF69572, SSF69572, 2 hits

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01408, Ube1, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00536, UBIQUITIN_ACTIVAT_1, 1 hit
PS00865, UBIQUITIN_ACTIVAT_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P22314-1) [UniParc]FASTAAdd to basket
Also known as: E1a

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSSPLSKKR RVSGPDPKPG SNCSPAQSVL SEVPSVPTNG MAKNGSEADI 
        60         70         80         90        100
DEGLYSRQLY VLGHEAMKRL QTSSVLVSGL RGLGVEIAKN IILGGVKAVT 
       110        120        130        140        150
LHDQGTAQWA DLSSQFYLRE EDIGKNRAEV SQPRLAELNS YVPVTAYTGP 
       160        170        180        190        200
LVEDFLSGFQ VVVLTNTPLE DQLRVGEFCH NRGIKLVVAD TRGLFGQLFC 
       210        220        230        240        250
DFGEEMILTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH GFESGDFVSF 
       260        270        280        290        300
SEVQGMVELN GNQPMEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK 
       310        320        330        340        350
ISFKSLVASL AEPDFVVTDF AKFSRPAQLH IGFQALHQFC AQHGRPPRPR 
       360        370        380        390        400
NEEDAAELVA LAQAVNARAL PAVQQNNLDE DLIRKLAYVA AGDLAPINAF 
       410        420        430        440        450
IGGLAAQEVM KACSGKFMPI MQWLYFDALE CLPEDKEVLT EDKCLQRQNR 
       460        470        480        490        500
YDGQVAVFGS DLQEKLGKQK YFLVGAGAIG CELLKNFAMI GLGCGEGGEI 
       510        520        530        540        550
IVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN PHIRVTSHQN 
       560        570        580        590        600
RVGPDTERIY DDDFFQNLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT 
       610        620        630        640        650
LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD 
       660        670        680        690        700
EFEGLFKQPA ENVNQYLTDP KFVERTLRLA GTQPLEVLEA VQRSLVLQRP 
       710        720        730        740        750
QTWADCVTWA CHHWHTQYSN NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH 
       760        770        780        790        800
PLTFDVNNPL HLDYVMAAAN LFAQTYGLTG SQDRAAVATF LQSVQVPEFT 
       810        820        830        840        850
PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP GFKMYPIDFE 
       860        870        880        890        900
KDDDSNFHMD FIVAASNLRA ENYDIPSADR HKSKLIAGKI IPAIATTTAA 
       910        920        930        940        950
VVGLVCLELY KVVQGHRQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ 
       960        970        980        990       1000
EWTLWDRFEV QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF 
      1010       1020       1030       1040       1050
FMPAAKLKER LDQPMTEIVS RVSKRKLGRH VRALVLELCC NDESGEDVEV 

PYVRYTIR
Length:1,058
Mass (Da):117,849
Last modified:October 25, 2002 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4B413AAA75FAA562
GO
Isoform 2 (identifier: P22314-2) [UniParc]FASTAAdd to basket
Also known as: E1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Show »
Length:1,018
Mass (Da):113,800
Checksum:i9A508347FE29FB19
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5JRR6Q5JRR6_HUMAN
Ubiquitin-like modifier-activating ...
UBA1
506Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5JRR9Q5JRR9_HUMAN
Ubiquitin-like modifier-activating ...
UBA1
270Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5JRS0Q5JRS0_HUMAN
Ubiquitin-like modifier-activating ...
UBA1
284Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5JRS3Q5JRS3_HUMAN
Ubiquitin-like modifier-activating ...
UBA1
195Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5JRS1Q5JRS1_HUMAN
Ubiquitin-like modifier-activating ...
UBA1
173Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5JRS2Q5JRS2_HUMAN
Ubiquitin-like modifier-activating ...
UBA1
234Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti190D → G in CAA40296 (PubMed:1606621).Curated1
Sequence conflicti434E → Q in CAA40296 (PubMed:1606621).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_043500447R → H. Corresponds to variant dbSNP:rs2070169EnsemblClinVar.1
Natural variantiVAR_043501539M → I in SMAX2. 1 PublicationCorresponds to variant dbSNP:rs80356545EnsemblClinVar.1
Natural variantiVAR_043502547S → G in SMAX2. 1 PublicationCorresponds to variant dbSNP:rs80356546EnsemblClinVar.1
Natural variantiVAR_071121557E → V in SMAX2. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0559131 – 40Missing in isoform 2. CuratedAdd BLAST40

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X56976 mRNA Translation: CAA40296.1
M58028 mRNA Translation: AAA61246.1
AL513366 Genomic DNA No translation available.
CH471164 Genomic DNA Translation: EAW59290.1
BC013041 mRNA Translation: AAH13041.1
X52897 mRNA Translation: CAA37078.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS14275.1 [P22314-1]

Protein sequence database of the Protein Information Resource

More...PIRi		A38564

NCBI Reference Sequences

More...RefSeqi		NP_003325.2, NM_003334.3 [P22314-1]
NP_695012.1, NM_153280.2 [P22314-1]
XP_016885269.1, XM_017029780.1 [P22314-1]
XP_016885270.1, XM_017029781.1 [P22314-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000335972; ENSP00000338413; ENSG00000130985 [P22314-1]
ENST00000377351; ENSP00000366568; ENSG00000130985 [P22314-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		7317

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:7317

UCSC genome browser

More...UCSCi		uc004dhj.5, human [P22314-1]

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56976 mRNA Translation: CAA40296.1
M58028 mRNA Translation: AAA61246.1
AL513366 Genomic DNA No translation available.
CH471164 Genomic DNA Translation: EAW59290.1
BC013041 mRNA Translation: AAH13041.1
X52897 mRNA Translation: CAA37078.1
CCDSiCCDS14275.1 [P22314-1]
PIRiA38564
RefSeqiNP_003325.2, NM_003334.3 [P22314-1]
NP_695012.1, NM_153280.2 [P22314-1]
XP_016885269.1, XM_017029780.1 [P22314-1]
XP_016885270.1, XM_017029781.1 [P22314-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P22X-ray2.75A/B1-439[»]
6DC6X-ray3.14A/C49-1058[»]
SMRiP22314
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi113165, 208 interactors
DIPiDIP-33686N
IntActiP22314, 152 interactors
MINTiP22314
STRINGi9606.ENSP00000338413

Chemistry databases

BindingDBiP22314
ChEMBLiCHEMBL5924
DrugBankiDB04119, Hexatantalum Dodecabromide
DB04216, Quercetin

PTM databases

iPTMnetiP22314
MetOSiteiP22314
PhosphoSitePlusiP22314
SwissPalmiP22314

Polymorphism and mutation databases

BioMutaiUBA1
DMDMi24418865

2D gel databases

REPRODUCTION-2DPAGEiIPI00645078

Proteomic databases

CPTACiCPTAC-136
CPTAC-137
EPDiP22314
jPOSTiP22314
MassIVEiP22314
MaxQBiP22314
PaxDbiP22314
PeptideAtlasiP22314
PRIDEiP22314
ProteomicsDBi53983 [P22314-1]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		345, 385 antibodies

The CPTC Antibody Portal

More...CPTCi		P22314, 3 antibodies

The DNASU plasmid repository

More...DNASUi		7317

Genome annotation databases

EnsembliENST00000335972; ENSP00000338413; ENSG00000130985 [P22314-1]
ENST00000377351; ENSP00000366568; ENSG00000130985 [P22314-1]
GeneIDi7317
KEGGihsa:7317
UCSCiuc004dhj.5, human [P22314-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		7317
DisGeNETi7317
EuPathDBiHostDB:ENSG00000130985.16

GeneCards: human genes, protein and diseases

More...GeneCardsi		UBA1
GeneReviewsiUBA1
HGNCiHGNC:12469, UBA1
HPAiENSG00000130985, Low tissue specificity
MalaCardsiUBA1
MIMi301830, phenotype
314370, gene
neXtProtiNX_P22314
OpenTargetsiENSG00000130985
Orphaneti1145, Infantile-onset X-linked spinal muscular atrophy
PharmGKBiPA37119

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2012, Eukaryota
GeneTreeiENSGT00940000158975
HOGENOMiCLU_002556_0_0_1
InParanoidiP22314
OMAiCIVWARL
OrthoDBi91748at2759
PhylomeDBiP22314
TreeFamiTF300586

Enzyme and pathway databases

UniPathwayiUPA00143
BioCyciMetaCyc:HS05465-MONOMER
BRENDAi2.3.2.B5, 2681
6.2.1.B9, 2681
PathwayCommonsiP22314
ReactomeiR-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiP22314

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		7317, 373 hits in 469 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		UBA1, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		UBA1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		7317
PharosiP22314, Tbio

Protein Ontology

More...PROi		PR:P22314
RNActiP22314, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000130985, Expressed in pituitary gland and 256 other tissues
ExpressionAtlasiP22314, baseline and differential
GenevisibleiP22314, HS

Family and domain databases

Gene3Di1.10.10.2660, 1 hit
2.40.30.180, 1 hit
3.10.290.60, 1 hit
3.50.50.80, 1 hit
InterProiView protein in InterPro
IPR032420, E1_4HB
IPR032418, E1_FCCH
IPR042302, E1_FCCH_sf
IPR000594, ThiF_NAD_FAD-bd
IPR018965, Ub-activating_enz_E1_C
IPR042449, Ub-E1_IAD_1
IPR038252, UBA_E1_C_sf
IPR019572, UBA_E1_Cys
IPR042063, Ubi_acti_E1_SCCH
IPR035985, Ubiquitin-activating_enz
IPR018075, UBQ-activ_enz_E1
IPR018074, UBQ-activ_enz_E1_CS
IPR033127, UBQ-activ_enz_E1_Cys_AS
IPR000011, UBQ/SUMO-activ_enz_E1-like
PfamiView protein in Pfam
PF16191, E1_4HB, 1 hit
PF16190, E1_FCCH, 1 hit
PF09358, E1_UFD, 1 hit
PF00899, ThiF, 2 hits
PF10585, UBA_e1_thiolCys, 1 hit
PRINTSiPR01849, UBIQUITINACT
SMARTiView protein in SMART
SM00985, UBA_e1_C, 1 hit
SUPFAMiSSF69572, SSF69572, 2 hits
TIGRFAMsiTIGR01408, Ube1, 1 hit
PROSITEiView protein in PROSITE
PS00536, UBIQUITIN_ACTIVAT_1, 1 hit
PS00865, UBIQUITIN_ACTIVAT_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUBA1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P22314
Secondary accession number(s): Q5JRR8, Q96E13
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 25, 2002
Last modified: December 2, 2020
This is version 228 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
  6. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  7. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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