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Protein

Bifunctional folate synthesis protein

Gene

sulD

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes two sequential steps of tetrahydrofolate biosynthesis, the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin diphosphate.1 Publication

Catalytic activityi

7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde.1 Publication
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.2 Publications

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 3 and 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Bifunctional folate synthesis protein (sulD)
  4. Bifunctional folate synthesis protein (sulD)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21SubstrateBy similarity1
Binding sitei53SubstrateBy similarity1
Active sitei99Proton donor/acceptor; for DHNA activityBy similarity1
Metal bindingi212Magnesium 1By similarity1
Metal bindingi212Magnesium 2By similarity1
Metal bindingi214Magnesium 1By similarity1
Metal bindingi214Magnesium 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi160 – 163ATPBy similarity4
Nucleotide bindingi171 – 173ATPBy similarity3
Nucleotide bindingi192 – 195ATPBy similarity4
Nucleotide bindingi200 – 215ATPBy similarityAdd BLAST16
Nucleotide bindingi227 – 233ATPBy similarity7
Nucleotide bindingi238 – 240ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Lyase, Multifunctional enzyme, Transferase
Biological processFolate biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPNE170187:G1FZB-301-MONOMER.
UniPathwayiUPA00077; UER00154.
UPA00077; UER00155.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional folate synthesis protein
Including the following 2 domains:
Dihydroneopterin aldolase1 Publication (EC:4.1.2.251 Publication)
Short name:
DHNA1 Publication
Alternative name(s):
7,8-dihydroneopterin aldolase
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.32 Publications)
Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase1 Publication
Short name:
PPPK1 Publication
7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase
Short name:
HPPK1 Publication
Gene namesi
Name:sulD1 Publication
Ordered Locus Names:SP_0292
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682441 – 270Bifunctional folate synthesis proteinAdd BLAST270

Interactioni

Subunit structurei

Homotrimer or homotetramer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
groSQ97NV32EBI-2207011,EBI-2206949

Protein-protein interaction databases

IntActiP22291. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliP22291.
SMRiP22291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 119DHNAAdd BLAST119
Regioni72 – 73Substrate bindingBy similarity2
Regioni120 – 270HPPKAdd BLAST151

Sequence similaritiesi

In the N-terminal section; belongs to the DHNA family.Curated
In the C-terminal section; belongs to the HPPK family.Curated

Phylogenomic databases

eggNOGiENOG4105K8U. Bacteria.
COG0801. LUCA.
COG1539. LUCA.
HOGENOMiHOG000019668.
KOiK13940.
OMAiIKKPWAP.

Family and domain databases

CDDicd00534. DHNA_DHNTPE. 1 hit.
cd00483. HPPK. 1 hit.
Gene3Di3.30.70.560. 1 hit.
InterProiView protein in InterPro
IPR006156. Dihydroneopterin_aldolase.
IPR006157. FolB_dom.
IPR000550. Hppk.
IPR035907. Hppk_sf.
PfamiView protein in Pfam
PF02152. FolB. 1 hit.
PF01288. HPPK. 1 hit.
SMARTiView protein in SMART
SM00905. FolB. 1 hit.
SUPFAMiSSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR00525. folB. 1 hit.
TIGR00526. folB_dom. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiView protein in PROSITE
PS00794. HPPK. 1 hit.

Sequencei

Sequence statusi: Complete.

P22291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQLQIKDLE MFAYHGLFPS EKELGQKFVV SAILSYDMTK AATDLDLTAS
60 70 80 90 100
VHYGELCQQW TTWFQETSED LIETVAYKLV ERTFEFYPLV QEMKLELKKP
110 120 130 140 150
WAPVHLSLDT CSVTIHRRKQ RAFIALGSNM GDKQANLKQA IDKLRARGIH
160 170 180 190 200
ILKESSVLAT EPWGGVEQDS FANQVVEVET WLPAQDLLET LLAIESELGR
210 220 230 240 250
VREVHWGPRL IDLDLLFVED QILYTDDLIL PHPYIAERLF VLESLQEIAP
260 270
HFIHPILKQP IRNLYDALKK
Length:270
Mass (Da):31,143
Last modified:September 26, 2001 - v3
Checksum:i1AAD0229E639A827
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29V → I in AAB63947 (PubMed:2168367).Curated1
Sequence conflicti86F → S in AAB63947 (PubMed:2168367).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16156 Genomic DNA. Translation: AAB63947.1.
AE005672 Genomic DNA. Translation: AAK74470.1.
PIRiA36704.
E95034.
E97905.
RefSeqiWP_000372529.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK74470; AAK74470; SP_0292.
KEGGispn:SP_0292.

Similar proteinsi

Entry informationi

Entry nameiSULD_STRPN
AccessioniPrimary (citable) accession number: P22291
Secondary accession number(s): O33697
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: September 26, 2001
Last modified: February 28, 2018
This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing