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Protein

Phosphoenolpyruvate carboxykinase (ATP)

Gene

pckA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mn2+UniRule annotation5 PublicationsNote: Binds 1 Mn2+ ion per subunit.UniRule annotation5 Publications

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Allosterically activated by calcium. It may represent the only case of a monomeric, allosteric enzyme.4 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=3.8 µM for manganese (at 31 degrees Celsius and pH 7.5)2 Publications
  2. KM=0.11 mM for calcium (at 31 degrees Celsius and pH 7.5)2 Publications
  3. KM=0.31 mM for OAA (at 31 degrees Celsius and pH 7.5)2 Publications
  4. KM=0.51 mM for OAA (at pH 7.5)2 Publications
  5. KM=1.4 mM for magnesium (at 31 degrees Celsius and pH 7.5)2 Publications
  6. KM=11 mM for PEP (at pH 7.5)2 Publications

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: gluconeogenesis

    This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei65SubstrateUniRule annotation1 Publication1
    <p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi149Calcium; via carbonyl oxygenBy similarity1
    Metal bindingi150Calcium; via carbonyl oxygenBy similarity1
    Metal bindingi152Calcium; via carbonyl oxygenBy similarity1
    Binding sitei207SubstrateUniRule annotation1 Publication1
    Metal bindingi213ManganeseUniRule annotation1 Publication1
    Binding sitei213ATPUniRule annotation6 Publications1
    Binding sitei213SubstrateUniRule annotation1 Publication1
    Metal bindingi232Manganese; via tele nitrogenUniRule annotation1 Publication1
    Binding sitei232ATPUniRule annotation6 Publications1
    Metal bindingi269ManganeseUniRule annotation1 Publication1
    Metal bindingi283Calcium; via carbonyl oxygenBy similarity1
    Binding sitei297ATPUniRule annotation6 Publications1
    Binding sitei333ATPUniRule annotation6 Publications1
    Binding sitei333SubstrateUniRule annotation1 Publication1
    Binding sitei455ATPUniRule annotation6 Publications1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi248 – 256ATPUniRule annotation6 Publications9
    Nucleotide bindingi449 – 450ATPUniRule annotation6 Publications2

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    • gluconeogenesis Source: EcoCyc

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAllosteric enzyme, Decarboxylase, Lyase
    Biological processGluconeogenesis
    LigandATP-binding, Calcium, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:PEPCARBOXYKIN-MONOMER
    MetaCyc:PEPCARBOXYKIN-MONOMER

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    4.1.1.49 2026

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00138

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase (ATP)UniRule annotation (EC:4.1.1.49UniRule annotation)
    Short name:
    PCKUniRule annotation
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:pckAUniRule annotation
    Synonyms:pck
    Ordered Locus Names:b3403, JW3366
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    Escherichia coli strain K12 genome database

    More...
    EcoGenei
    EG10688 pck

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi65R → Q: Slightly lower catalytic efficiency compared to wild-type and the affinity binding for OAA is 330-fold higher than for wild-type. 1 Publication1
    Mutagenesisi268D → N in PCK51; altered-activity mutant that catalyzes the conversion from oxaloacetate to pyruvate (OAA decarboxylase activity). 1 Publication1
    Mutagenesisi284G → S in PCK53; shows reduced-activity. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002038181 – 540Phosphoenolpyruvate carboxykinase (ATP)Add BLAST540

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei87N6-acetyllysineUniRule annotation1 Publication1
    Modified residuei523N6-acetyllysineUniRule annotation1 Publication1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    Encyclopedia of Proteome Dynamics

    More...
    EPDi
    P22259

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P22259

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P22259

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    P22259

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Induced upon entry into stationary phase and by cyclic AMP (cAMP). Repressed by glucose (catabolite repression) and by CsrA.2 Publications

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.UniRule annotation9 Publications

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...
    BioGridi
    4262181, 19 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    P22259, 4 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    316385.ECDH10B_3578

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1540
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

    More...
    ProteinModelPortali
    P22259

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P22259

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    Miscellaneous databases

    Relative evolutionary importance of amino acids within a protein sequence

    More...
    EvolutionaryTracei
    P22259

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.UniRule annotation

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105DJ1 Bacteria
    COG1866 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000271471

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P22259

    KEGG Orthology (KO)

    More...
    KOi
    K01610

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P22259

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00484 PEPCK_ATP, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.449.10, 1 hit
    3.90.228.20, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_00453 PEPCK_ATP, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR001272 PEP_carboxykinase_ATP
    IPR013035 PEP_carboxykinase_C
    IPR008210 PEP_carboxykinase_N
    IPR015994 PEPCK_ATP_CS

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR30031 PTHR30031, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF01293 PEPCK_ATP, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF006294 PEP_crbxkin, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF68923 SSF68923, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00224 pckA, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00532 PEPCK_ATP, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P22259-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MRVNNGLTPQ ELEAYGISDV HDIVYNPSYD LLYQEELDPS LTGYERGVLT
    60 70 80 90 100
    NLGAVAVDTG IFTGRSPKDK YIVRDDTTRD TFWWADKGKG KNDNKPLSPE
    110 120 130 140 150
    TWQHLKGLVT RQLSGKRLFV VDAFCGANPD TRLSVRFITE VAWQAHFVKN
    160 170 180 190 200
    MFIRPSDEEL AGFKPDFIVM NGAKCTNPQW KEQGLNSENF VAFNLTERMQ
    210 220 230 240 250
    LIGGTWYGGE MKKGMFSMMN YLLPLKGIAS MHCSANVGEK GDVAVFFGLS
    260 270 280 290 300
    GTGKTTLSTD PKRRLIGDDE HGWDDDGVFN FEGGCYAKTI KLSKEAEPEI
    310 320 330 340 350
    YNAIRRDALL ENVTVREDGT IDFDDGSKTE NTRVSYPIYH IDNIVKPVSK
    360 370 380 390 400
    AGHATKVIFL TADAFGVLPP VSRLTADQTQ YHFLSGFTAK LAGTERGITE
    410 420 430 440 450
    PTPTFSACFG AAFLSLHPTQ YAEVLVKRMQ AAGAQAYLVN TGWNGTGKRI
    460 470 480 490 500
    SIKDTRAIID AILNGSLDNA ETFTLPMFNL AIPTELPGVD TKILDPRNTY
    510 520 530 540
    ASPEQWQEKA ETLAKLFIDN FDKYTDTPAG AALVAAGPKL
    Length:540
    Mass (Da):59,643
    Last modified:November 1, 1995 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA744D90899F6A8A5
    GO

    <p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

    The sequence AAA24301 differs from that shown. Reason: Frameshift at position 456. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti67 – 72PKDKYI → QKISIS in AAA24301 (PubMed:1701430).Curated6
    Sequence conflicti192 – 194AFN → R AA sequence (PubMed:1701430).Curated3
    Sequence conflicti197 – 203ERMQLIG → DRAHAADC AA sequence (PubMed:1701430).Curated7
    Sequence conflicti233C → S in AAA24301 (PubMed:1701430).Curated1
    Sequence conflicti252T → N in AAA24301 (PubMed:1701430).Curated1
    Sequence conflicti256 – 260TLSTD → AFPR in AAA24301 (PubMed:1701430).Curated5
    Sequence conflicti281 – 285FEGGC → LKAAG in AAA24301 (PubMed:1701430).Curated5
    Sequence conflicti302 – 307NAIRRD → KLSVVM in AAA24301 (PubMed:1701430).Curated6
    Sequence conflicti346K → R in AAA24301 (PubMed:1701430).Curated1
    Sequence conflicti354 – 359ATKVIF → GRRLSL in AAA24301 (PubMed:1701430).Curated6
    Sequence conflicti363D → H in AAA24301 (PubMed:1701430).Curated1
    Sequence conflicti446T → S in AAA24301 (PubMed:1701430).Curated1
    Sequence conflicti449R → S in AAA24301 (PubMed:1701430).Curated1
    Sequence conflicti460 – 467DAILNGSL → RRHPQRFV (PubMed:6292200).Curated8
    Sequence conflicti499T → R (PubMed:6292200).Curated1

    <p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

    Molecular mass is 59656 Da from positions 1 - 540. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    M59823 Genomic DNA Translation: AAA24301.1 Frameshift.
    U18997 Genomic DNA Translation: AAA58200.1
    U00096 Genomic DNA Translation: AAC76428.1
    AP009048 Genomic DNA Translation: BAE77888.1
    J01656 Unassigned RNA No translation available.
    S76268 Genomic DNA Translation: AAB33745.2
    S76269 Genomic DNA Translation: AAB33746.2
    U21325 Genomic DNA Translation: AAA95999.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    F65135

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_417862.1, NC_000913.3
    WP_001265681.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC76428; AAC76428; b3403
    BAE77888; BAE77888; BAE77888

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    945667

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW3366
    eco:b3403

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|1411691.4.peg.3326

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M59823 Genomic DNA Translation: AAA24301.1 Frameshift.
    U18997 Genomic DNA Translation: AAA58200.1
    U00096 Genomic DNA Translation: AAC76428.1
    AP009048 Genomic DNA Translation: BAE77888.1
    J01656 Unassigned RNA No translation available.
    S76268 Genomic DNA Translation: AAB33745.2
    S76269 Genomic DNA Translation: AAB33746.2
    U21325 Genomic DNA Translation: AAA95999.1
    PIRiF65135
    RefSeqiNP_417862.1, NC_000913.3
    WP_001265681.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1AQ2X-ray1.90A1-540[»]
    1AYLX-ray1.80A1-540[»]
    1K3CX-ray2.00A1-540[»]
    1K3DX-ray2.00A1-540[»]
    1OENX-ray1.90A1-538[»]
    1OS1X-ray1.80A1-540[»]
    2OLQX-ray1.94A1-540[»]
    2OLRX-ray1.60A1-540[»]
    2PXZX-ray2.23X1-540[»]
    2PY7X-ray2.20X1-540[»]
    6ASIX-ray1.79A1-540[»]
    6ASMX-ray1.55A1-540[»]
    6ASNX-ray1.55A1-540[»]
    6AT2X-ray1.44A1-540[»]
    6AT3X-ray1.46A/B1-540[»]
    6AT4X-ray1.33A/B1-540[»]
    6COMX-ray2.30A1-540[»]
    6CRTX-ray2.00A1-540[»]
    6CU4X-ray1.80A1-540[»]
    6D5IX-ray1.80A10-540[»]
    ProteinModelPortaliP22259
    SMRiP22259
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262181, 19 interactors
    IntActiP22259, 4 interactors
    STRINGi316385.ECDH10B_3578

    PTM databases

    iPTMnetiP22259

    Proteomic databases

    EPDiP22259
    PaxDbiP22259
    PRIDEiP22259

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76428; AAC76428; b3403
    BAE77888; BAE77888; BAE77888
    GeneIDi945667
    KEGGiecj:JW3366
    eco:b3403
    PATRICifig|1411691.4.peg.3326

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB0682
    EcoGeneiEG10688 pck

    Phylogenomic databases

    eggNOGiENOG4105DJ1 Bacteria
    COG1866 LUCA
    HOGENOMiHOG000271471
    InParanoidiP22259
    KOiK01610
    PhylomeDBiP22259

    Enzyme and pathway databases

    UniPathwayi
    UPA00138

    BioCyciEcoCyc:PEPCARBOXYKIN-MONOMER
    MetaCyc:PEPCARBOXYKIN-MONOMER
    BRENDAi4.1.1.49 2026

    Miscellaneous databases

    EvolutionaryTraceiP22259

    Protein Ontology

    More...
    PROi
    PR:P22259

    Family and domain databases

    CDDicd00484 PEPCK_ATP, 1 hit
    Gene3Di3.40.449.10, 1 hit
    3.90.228.20, 1 hit
    HAMAPiMF_00453 PEPCK_ATP, 1 hit
    InterProiView protein in InterPro
    IPR001272 PEP_carboxykinase_ATP
    IPR013035 PEP_carboxykinase_C
    IPR008210 PEP_carboxykinase_N
    IPR015994 PEPCK_ATP_CS
    PANTHERiPTHR30031 PTHR30031, 1 hit
    PfamiView protein in Pfam
    PF01293 PEPCK_ATP, 1 hit
    PIRSFiPIRSF006294 PEP_crbxkin, 1 hit
    SUPFAMiSSF68923 SSF68923, 1 hit
    TIGRFAMsiTIGR00224 pckA, 1 hit
    PROSITEiView protein in PROSITE
    PS00532 PEPCK_ATP, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPCKA_ECOLI
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P22259
    Secondary accession number(s): Q2M768
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: November 1, 1995
    Last modified: December 5, 2018
    This is version 171 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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