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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per subunit.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108ZincUniRule annotation1
Metal bindingi110ZincUniRule annotation1
Metal bindingi135ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Binding sitei255ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU00920-MONOMER
MetaCyc:MONOMER-13959
SABIO-RKiP22250

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:BSU00920
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195081 – 483Glutamate--tRNA ligaseAdd BLAST483

Proteomic databases

PaxDbiP22250
PRIDEiP22250

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiP22250, 1 interactor
MINTiP22250
STRINGi224308.Bsubs1_010100000475

Structurei

3D structure databases

ProteinModelPortaliP22250
SMRiP22250
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi252 – 256"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
InParanoidiP22250
KOiK09698
OMAiAYYAFDT
PhylomeDBiP22250

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P22250-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGNEVRVRYA PSPTGHLHIG NARTALFNYL FARNQGGKFI IRVEDTDKKR
60 70 80 90 100
NIEGGEQSQL NYLKWLGIDW DESVDVGGEY GPYRQSERND IYKVYYEELL
110 120 130 140 150
EKGLAYKCYC TEEELEKERE EQIARGEMPR YSGKHRDLTQ EEQEKFIAEG
160 170 180 190 200
RKPSIRFRVP EGKVIAFNDI VKGEISFESD GIGDFVIVKK DGTPTYNFAV
210 220 230 240 250
AIDDYLMKMT HVLRGEDHIS NTPKQIMIYQ AFGWDIPQFG HMTLIVNESR
260 270 280 290 300
KKLSKRDESI IQFIEQYKEL GYLPEALFNF IGLLGWSPVG EEELFTKEQF
310 320 330 340 350
IEIFDVNRLS KSPALFDMHK LKWVNNQYVK KLDLDQVVEL TLPHLQKAGK
360 370 380 390 400
VGTELSAEEQ EWVRKLISLY HEQLSYGAEI VELTDLFFTD EIEYNQEAKA
410 420 430 440 450
VLEEEQVPEV LSTFAAKLEE LEEFTPDNIK ASIKAVQKET GHKGKKLFMP
460 470 480
IRVAVTGQTH GPELPQSIEL IGKETAIQRL KNI
Length:483
Mass (Da):55,722
Last modified:August 1, 1991 - v1
Checksum:iEF61671AEE465DD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55073 Genomic DNA Translation: AAA22495.1
L14580 Genomic DNA Translation: AAA21796.1
D26185 Genomic DNA Translation: BAA05326.1
U49789 Genomic DNA Translation: AAC31971.1
AL009126 Genomic DNA Translation: CAB11868.1
PIRiA36090 SYBSET
RefSeqiNP_387973.1, NC_000964.3
WP_004399675.1, NZ_JNCM01000029.1

Genome annotation databases

EnsemblBacteriaiCAB11868; CAB11868; BSU00920
GeneIDi936862
KEGGibsu:BSU00920
PATRICifig|224308.179.peg.93

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55073 Genomic DNA Translation: AAA22495.1
L14580 Genomic DNA Translation: AAA21796.1
D26185 Genomic DNA Translation: BAA05326.1
U49789 Genomic DNA Translation: AAC31971.1
AL009126 Genomic DNA Translation: CAB11868.1
PIRiA36090 SYBSET
RefSeqiNP_387973.1, NC_000964.3
WP_004399675.1, NZ_JNCM01000029.1

3D structure databases

ProteinModelPortaliP22250
SMRiP22250
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP22250, 1 interactor
MINTiP22250
STRINGi224308.Bsubs1_010100000475

Proteomic databases

PaxDbiP22250
PRIDEiP22250

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11868; CAB11868; BSU00920
GeneIDi936862
KEGGibsu:BSU00920
PATRICifig|224308.179.peg.93

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
InParanoidiP22250
KOiK09698
OMAiAYYAFDT
PhylomeDBiP22250

Enzyme and pathway databases

BioCyciBSUB:BSU00920-MONOMER
MetaCyc:MONOMER-13959
SABIO-RKiP22250

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_BACSU
AccessioniPrimary (citable) accession number: P22250
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 10, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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