UniProtKB - P22227 (ZFP42_MOUSE)
Protein
Zinc finger protein 42
Gene
Zfp42
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 170 – 194 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 199 – 221 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 227 – 251 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 258 – 281 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA binding Source: GO_Central
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: MGI
- in utero embryonic development Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of genetic imprinting Source: MGI
- spermatid development Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein 42Short name: Zfp-42 Alternative name(s): Reduced expression protein 1 Short name: REX-1 Short name: mREX-1 |
Gene namesi | Name:Zfp42 Synonyms:Rex-1, Rex1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99187, Zfp42 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nuclear chromatin Source: GO_Central
- nucleus Source: MGI
- PcG protein complex Source: GO_Central
Other locations
- cytoplasm Source: MGI
- protein-containing complex Source: MGI
- transcription regulator complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047300 | 1 – 288 | Zinc finger protein 42Add BLAST | 288 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 213 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 215 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Polyubiquitinated by RNF12, leading to proteasomal degradation.1 Publication
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
jPOSTi | P22227 |
MaxQBi | P22227 |
PaxDbi | P22227 |
PeptideAtlasi | P22227 |
PRIDEi | P22227 |
PTM databases
iPTMneti | P22227 |
PhosphoSitePlusi | P22227 |
Expressioni
Tissue specificityi
Restricted to testis, to germ cells in the early stages of spermatogenesis. Not expressed in spermatids, nor spermatozoa. Expressed in embryonic stem (ES) cells.4 Publications
Developmental stagei
Expressed in the inner cell mass of 4.5 dpc blastocysts, as well as in the polar trophoblast. At 6 dpc, abundant in the extraembryonic ectoderm and the ectoplacental cone. At this stage, not detected in the embryonic ectoderm. At 7 dpc, restricted to the extraembryonic ectoderm and the ectoplacental cone. Also expressed in the placenta. Expressed in male germ cells undergoing meiosis.1 Publication
Inductioni
Up-regulated by NANOG, up-regulation which is increased by SOX2 and POU5F1. Up-regulated by nicotine; up-regulation which is prevented with tubocurarine, a nicotinic acetylcholine receptor antagonist. Down-regulated by sodium vanadate and retinoic acid (RA). In ES cells, down-regulation correlates with differentiation.5 Publications
Interactioni
Binary interactionsi
P22227
With | #Exp. | IntAct |
---|---|---|
Rlim [Q9WTV7] | 8 | EBI-2313372,EBI-15657872 |
Protein-protein interaction databases
BioGRIDi | 204664, 16 interactors |
DIPi | DIP-54911N |
IntActi | P22227, 3 interactors |
MINTi | P22227 |
STRINGi | 10090.ENSMUSP00000080765 |
Miscellaneous databases
RNActi | P22227, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 56 – 103 | Asp/Glu-rich (acidic)Add BLAST | 48 |
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 170 – 194 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 199 – 221 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 227 – 251 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 258 – 281 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
InParanoidi | P22227 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P22227 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P22227-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNEQKMNEQM KKTAKTSGQK GPGGRALDRL TLKQDEARPV QNTRVEAPRV
60 70 80 90 100
TYTIRDESEI SPETEEDGFP DGYLECIIRG EFSEPILEED FLFKSFESLE
110 120 130 140 150
EVEQNLSRQV LEASSLLESS LEYMTKGTKQ EKTEVTQETP PLRVGASSLL
160 170 180 190 200
AGGPAEKPEG GVYCGVLSML ECPQAGCKKK LRGKTALRKH MLVHGPRRHV
210 220 230 240 250
CAECGKAFTE SSKLKRHFLV HTGEKPYQCT FEGCGKRFSL DFNLRTHIRI
260 270 280
HTGERRFVCP FDGCEKSFIQ SNNQKIHILT HAKAGKKC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9QK22 | E9QK22_MOUSE | Zinc finger protein 42 | Zfp42 | 288 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 | T → A in AAH53399 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 133 | T → R in AAH53399 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 155 | A → T in AAH53399 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 183 | G → D in AAH53399 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28382 mRNA Translation: AAA40053.1 M97812 Genomic DNA Translation: AAA19700.1 BC053399 mRNA Translation: AAH53399.1 BC098467 mRNA Translation: AAH98467.1 |
CCDSi | CCDS22267.1 |
PIRi | A33828 |
RefSeqi | NP_033582.2, NM_009556.3 |
Genome annotation databases
GeneIDi | 22702 |
KEGGi | mmu:22702 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28382 mRNA Translation: AAA40053.1 M97812 Genomic DNA Translation: AAA19700.1 BC053399 mRNA Translation: AAH53399.1 BC098467 mRNA Translation: AAH98467.1 |
CCDSi | CCDS22267.1 |
PIRi | A33828 |
RefSeqi | NP_033582.2, NM_009556.3 |
3D structure databases
SMRi | P22227 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204664, 16 interactors |
DIPi | DIP-54911N |
IntActi | P22227, 3 interactors |
MINTi | P22227 |
STRINGi | 10090.ENSMUSP00000080765 |
PTM databases
iPTMneti | P22227 |
PhosphoSitePlusi | P22227 |
Proteomic databases
jPOSTi | P22227 |
MaxQBi | P22227 |
PaxDbi | P22227 |
PeptideAtlasi | P22227 |
PRIDEi | P22227 |
Genome annotation databases
GeneIDi | 22702 |
KEGGi | mmu:22702 |
Organism-specific databases
CTDi | 132625 |
MGIi | MGI:99187, Zfp42 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
InParanoidi | P22227 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P22227 |
Miscellaneous databases
BioGRID-ORCSi | 22702, 0 hits in 17 CRISPR screens |
PROi | PR:P22227 |
RNActi | P22227, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZFP42_MOUSE | |
Accessioni | P22227Primary (citable) accession number: P22227 Secondary accession number(s): Q4KMN3, Q7TPU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families