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Protein

Serine/threonine-protein kinase RAD53

Gene

RAD53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.5 Publications

Miscellaneous

Present with 6900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei227ATPPROSITE-ProRule annotation1
Active sitei319Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 212ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • deoxyribonucleoside triphosphate biosynthetic process Source: SGD
  • DNA damage checkpoint Source: SGD
  • DNA repair Source: SGD
  • DNA replication initiation Source: SGD
  • intracellular signal transduction Source: GO_Central
  • negative regulation of phosphorylation Source: SGD
  • nucleobase-containing compound metabolic process Source: SGD
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • protein localization Source: SGD
  • protein phosphorylation Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
Biological processCell cycle, DNA damage
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34050-MONOMER
BRENDAi2.7.12.1 984
ReactomeiR-SCE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-SCE-69473 G2/M DNA damage checkpoint
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RAD53 (EC:2.7.12.1)
Alternative name(s):
CHEK2 homolog
Serine-protein kinase 1
Gene namesi
Name:RAD53
Synonyms:MEC2, SAD1, SPK1
Ordered Locus Names:YPL153C
ORF Names:P2588
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL153C
SGDiS000006074 RAD53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865951 – 821Serine/threonine-protein kinase RAD53Add BLAST821

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineCombined sources1
Modified residuei175PhosphoserineCombined sources1
Modified residuei547PhosphoserineCombined sources1
Modified residuei560PhosphoserineCombined sources1
Modified residuei774PhosphoserineCombined sources1
Modified residuei793PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22216
PaxDbiP22216
PRIDEiP22216

PTM databases

iPTMnetiP22216

Interactioni

Subunit structurei

Interacts with PIN4.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36030, 583 interactors
ComplexPortaliCPX-1322 RAD53-ASF1 complex
DIPiDIP-2322N
IntActiP22216, 53 interactors
MINTiP22216
STRINGi4932.YPL153C

Structurei

Secondary structure

1821
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 22Combined sources7
Beta strandi31 – 43Combined sources13
Beta strandi46 – 50Combined sources5
Helixi52 – 57Combined sources6
Beta strandi59 – 61Combined sources3
Beta strandi64 – 71Combined sources8
Beta strandi74 – 77Combined sources4
Beta strandi82 – 84Combined sources3
Beta strandi89 – 93Combined sources5
Helixi95 – 97Combined sources3
Beta strandi99 – 103Combined sources5
Beta strandi110 – 115Combined sources6
Beta strandi122 – 124Combined sources3
Beta strandi129 – 132Combined sources4
Helixi137 – 139Combined sources3
Beta strandi141 – 147Combined sources7
Helixi149 – 154Combined sources6
Beta strandi160 – 162Combined sources3
Helixi193 – 196Combined sources4
Beta strandi197 – 201Combined sources5
Beta strandi211 – 217Combined sources7
Turni218 – 220Combined sources3
Beta strandi223 – 229Combined sources7
Helixi232 – 234Combined sources3
Helixi241 – 248Combined sources8
Beta strandi259 – 264Combined sources6
Beta strandi269 – 273Combined sources5
Helixi281 – 288Combined sources8
Helixi293 – 312Combined sources20
Helixi322 – 324Combined sources3
Beta strandi325 – 329Combined sources5
Turni330 – 333Combined sources4
Beta strandi334 – 337Combined sources4
Helixi359 – 361Combined sources3
Helixi364 – 367Combined sources4
Turni384 – 386Combined sources3
Helixi387 – 403Combined sources17
Helixi413 – 421Combined sources9
Helixi428 – 432Combined sources5
Helixi437 – 446Combined sources10
Helixi451 – 453Combined sources3
Helixi457 – 461Combined sources5
Turni464 – 467Combined sources4
Helixi484 – 493Combined sources10
Beta strandi551 – 557Combined sources7
Helixi563 – 565Combined sources3
Beta strandi572 – 575Combined sources4
Beta strandi578 – 582Combined sources5
Beta strandi586 – 588Combined sources3
Beta strandi592 – 597Combined sources6
Beta strandi601 – 606Combined sources6
Beta strandi609 – 612Combined sources4
Beta strandi620 – 629Combined sources10
Beta strandi636 – 638Combined sources3
Beta strandi645 – 651Combined sources7
Beta strandi653 – 655Combined sources3
Beta strandi657 – 659Combined sources3
Beta strandi662 – 664Combined sources3
Beta strandi666 – 671Combined sources6
Beta strandi678 – 680Combined sources3
Turni684 – 687Combined sources4
Beta strandi688 – 691Combined sources4
Beta strandi692 – 695Combined sources4
Beta strandi703 – 707Combined sources5
Beta strandi710 – 712Combined sources3
Beta strandi716 – 718Combined sources3
Helixi721 – 728Combined sources8
Beta strandi804 – 806Combined sources3
Helixi811 – 813Combined sources3

3D structure databases

ProteinModelPortaliP22216
SMRiP22216
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22216

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 116FHA 1PROSITE-ProRule annotationAdd BLAST51
Domaini198 – 466Protein kinasePROSITE-ProRule annotationAdd BLAST269
Domaini601 – 664FHA 2PROSITE-ProRule annotationAdd BLAST64

Domaini

FHA domains are phosphothreonine recognition modules, FHA 1 strongly selects for Asp at position +3 relative to phosphothreonine, whereas FHA 2 selects for Ile in this position.

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00800000124190
HOGENOMiHOG000074515
InParanoidiP22216
KOiK02831
OMAiCRLICTT
OrthoDBiEOG092C2FDZ

Family and domain databases

CDDicd00060 FHA, 2 hits
InterProiView protein in InterPro
IPR000253 FHA_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR016256 Ser/Thr_kinase_Rad53
IPR008984 SMAD_FHA_dom_sf
PfamiView protein in Pfam
PF00498 FHA, 2 hits
PF00069 Pkinase, 1 hit
PIRSFiPIRSF000661 Ser/Thr_PK_RAD53, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 2 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF49879 SSF49879, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P22216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA
60 70 80 90 100
DISQVLKEKR SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL
110 120 130 140 150
LNDISTNGTW LNGQKVEKNS NQLLSQGDEI TVGVGVESDI LSLVIFINDK
160 170 180 190 200
FKQCLEQNKV DRIRSNLKNT SKIASPGLTS STASSMVANK TGIFKDFSII
210 220 230 240 250
DEVVGQGAFA TVKKAIERTT GKTFAVKIIS KRKVIGNMDG VTRELEVLQK
260 270 280 290 300
LNHPRIVRLK GFYEDTESYY MVMEFVSGGD LMDFVAAHGA VGEDAGREIS
310 320 330 340 350
RQILTAIKYI HSMGISHRDL KPDNILIEQD DPVLVKITDF GLAKVQGNGS
360 370 380 390 400
FMKTFCGTLA YVAPEVIRGK DTSVSPDEYE ERNEYSSLVD MWSMGCLVYV
410 420 430 440 450
ILTGHLPFSG STQDQLYKQI GRGSYHEGPL KDFRISEEAR DFIDSLLQVD
460 470 480 490 500
PNNRSTAAKA LNHPWIKMSP LGSQSYGDFS QISLSQSLSQ QKLLENMDDA
510 520 530 540 550
QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKIPAHAPI RYTQPKSIEA
560 570 580 590 600
ETREQKLLHS NNTENVKSSK KKGNGRFLTL KPLPDSIIQE SLEIQQGVNP
610 620 630 640 650
FFIGRSEDCN CKIEDNRLSR VHCFIFKKRH AVGKSMYESP AQGLDDIWYC
660 670 680 690 700
HTGTNVSYLN NNRMIQGTKF LLQDGDEIKI IWDKNNKFVI GFKVEINDTT
710 720 730 740 750
GLFNEGLGML QEQRVVLKQT AEEKDLVKKL TQMMAAQRAN QPSASSSSMS
760 770 780 790 800
AKKPPVSDTN NNGNNSVLND LVESPINANT GNILKRIHSV SLSQSQIDPS
810 820
KKVKRAKLDQ TSKGPENLQF S
Length:821
Mass (Da):91,962
Last modified:August 1, 1991 - v1
Checksum:i84A9612229CA72D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204V → A in AAT93028 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55623 Genomic DNA Translation: AAA35070.1
X96770 Genomic DNA Translation: CAA65568.1
Z73509 Genomic DNA Translation: CAA97858.1
AY693009 Genomic DNA Translation: AAT93028.1
BK006949 Genomic DNA Translation: DAA11281.1
PIRiA39616
RefSeqiNP_015172.1, NM_001183967.1

Genome annotation databases

EnsemblFungiiYPL153C; YPL153C; YPL153C
GeneIDi855950
KEGGisce:YPL153C

Similar proteinsi

Entry informationi

Entry nameiRAD53_YEAST
AccessioniPrimary (citable) accession number: P22216
Secondary accession number(s): D6W3L5, Q6B1S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 18, 2018
This is version 205 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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