UniProtKB - P22196 (PER2_ARAHY)
Protein
Cationic peroxidase 2
Gene
PNC2
Organism
Arachis hypogaea (Peanut)
Status
Functioni
Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Catalytic activityi
- EC:1.11.1.7
Cofactori
Protein has several cofactor binding sites:- Ca2+Note: Binds 2 calcium ions per subunit.
- heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 68 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Active sitei | 72 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 73 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 76 | Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 78 | Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 80 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 82 | Calcium 1PROSITE-ProRule annotation | 1 | |
Binding sitei | 164 | Substrate; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 194 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 195 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 246 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 249 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 254 | Calcium 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: UniProtKB-KW
GO - Biological processi
- hydrogen peroxide catabolic process Source: UniProtKB-KW
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Ligand | Calcium, Heme, Iron, Metal-binding |
Protein family/group databases
PeroxiBasei | 103, AhPrx05 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PNC2 |
Organismi | Arachis hypogaea (Peanut) |
Taxonomic identifieri | 3818 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › dalbergioids sensu lato › Dalbergieae › Pterocarpus clade › Arachis |
Subcellular locationi
Extracellular region or secreted
- Secreted PROSITE-ProRule annotation
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000023747 | 24 – 330 | Cationic peroxidase 2Add BLAST | 307 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 117 | PROSITE-ProRule annotation | ||
Disulfide bondi | 74 ↔ 79 | PROSITE-ProRule annotation | ||
Disulfide bondi | 123 ↔ 326 | PROSITE-ProRule annotation | ||
Disulfide bondi | 201 ↔ 233 | PROSITE-ProRule annotation | ||
Glycosylationi | 212 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinFamily & Domainsi
Sequence similaritiesi
Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00693, secretory_peroxidase, 1 hit |
InterProi | View protein in InterPro IPR002016, Haem_peroxidase IPR010255, Haem_peroxidase_sf IPR000823, Peroxidase_pln IPR019794, Peroxidases_AS IPR019793, Peroxidases_heam-ligand_BS IPR033905, Secretory_peroxidase |
Pfami | View protein in Pfam PF00141, peroxidase, 1 hit |
PRINTSi | PR00458, PEROXIDASE PR00461, PLPEROXIDASE |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS00435, PEROXIDASE_1, 1 hit PS00436, PEROXIDASE_2, 1 hit PS50873, PEROXIDASE_4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P22196-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGVFNNKKF ILVFVFMLGL CIGITTVHGQ GTRVGFYSRT CPRAESIVRS
60 70 80 90 100
TVRSHVNSDP TLAAKILRMH FHDCFVQGCD GSILISGPAT EKTAFANLGL
110 120 130 140 150
RGYEIIDDAK TQLEAACPGV VSCADILALA ARDSVVLSGG LSWQVPTGRR
160 170 180 190 200
DGRVSQASDV SNLPAPSDSV DVQKQKFAAK GLNTQDLVTL VGGHTIGTSE
210 220 230 240 250
CQFFSNRLFN FNGTAAADPA IDPSFVSNLQ ALCPQNTGAA NRVALDTGSQ
260 270 280 290 300
FKFDTSYFSN LRNRRGVLQS DQALWNDPST KSFVQRYLGL RGFLGLTFNV
310 320 330
EFGKSMVKMS NIGVKTGTDG EIRKICSAFN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37637 mRNA Translation: AAA32676.1 |
PIRi | B38265 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37637 mRNA Translation: AAA32676.1 |
PIRi | B38265 |
3D structure databases
SMRi | P22196 |
ModBasei | Search... |
Protein family/group databases
PeroxiBasei | 103, AhPrx05 |
Family and domain databases
CDDi | cd00693, secretory_peroxidase, 1 hit |
InterProi | View protein in InterPro IPR002016, Haem_peroxidase IPR010255, Haem_peroxidase_sf IPR000823, Peroxidase_pln IPR019794, Peroxidases_AS IPR019793, Peroxidases_heam-ligand_BS IPR033905, Secretory_peroxidase |
Pfami | View protein in Pfam PF00141, peroxidase, 1 hit |
PRINTSi | PR00458, PEROXIDASE PR00461, PLPEROXIDASE |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS00435, PEROXIDASE_1, 1 hit PS00436, PEROXIDASE_2, 1 hit PS50873, PEROXIDASE_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PER2_ARAHY | |
Accessioni | P22196Primary (citable) accession number: P22196 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |