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Protein

Double-strand-specific pac1 ribonuclease

Gene

pac1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 8.5. No activity at pH below 6.5 or above 9.5.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding
Biological processMeiosis
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand-specific pac1 ribonuclease (EC:3.1.26.3)
Alternative name(s):
Protein hcs
Gene namesi
Name:pac1
Synonyms:hcs
ORF Names:SPBC119.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC119.11c
PomBaseiSPBC119.11c pac1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi178G → D or S in snm1-1 and ran1-114; loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001804671 – 363Double-strand-specific pac1 ribonucleaseAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22192
PaxDbiP22192
PRIDEiP22192

PTM databases

iPTMnetiP22192

Interactioni

Protein-protein interaction databases

BioGridi276622, 2 interactors
STRINGi4896.SPBC119.11c.1

Structurei

3D structure databases

ProteinModelPortaliP22192
SMRiP22192
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 262RNase IIIPROSITE-ProRule annotationAdd BLAST124
Domaini285 – 356DRBMPROSITE-ProRule annotationAdd BLAST72

Phylogenomic databases

InParanoidiP22192
KOiK03685
OMAiWGANQKD
OrthoDBiEOG092C2YRH
PhylomeDBiP22192

Family and domain databases

CDDicd00048 DSRM, 1 hit
cd00593 RIBOc, 1 hit
Gene3Di1.10.1520.10, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF00636 Ribonuclease_3, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00535 RIBOc, 1 hit
SUPFAMiSSF69065 SSF69065, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 1 hit

Sequencei

Sequence statusi: Complete.

P22192-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRFKRHHEG DSDSSSSASD SLSRGRRSLG HKRSSHIKNR QYYILEKKIR
60 70 80 90 100
KLMFAMKALL EETKHSTKDD VNLVIPGSTW SHIEGVYEML KSRHDRQNEP
110 120 130 140 150
VIEEPSSHPK NQKNQENNEP TSEEFEEGEY PPPLPPLRSE KLKEQVFMHI
160 170 180 190 200
SRAYEIYPNQ SNPNELLDIH NERLEFLGDS FFNLFTTRII FSKFPQMDEG
210 220 230 240 250
SLSKLRAKFV GNESADKFAR LYGFDKTLVL SYSAEKDQLR KSQKVIADTF
260 270 280 290 300
EAYLGALILD GQEETAFQWV SRLLQPKIAN ITVQRPIDKL AKSKLFHKYS
310 320 330 340 350
TLGHIEYRWV DGAGGSAEGY VIACIFNGKE VARAWGANQK DAGSRAAMQA
360
LEVLAKDYSK FAR
Length:363
Mass (Da):41,539
Last modified:August 1, 1991 - v1
Checksum:iBB866CD6AC5AF33A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144E → K in AAU05314 (PubMed:7616961).Curated1
Sequence conflicti144E → K in AAU05315 (PubMed:7616961).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54998 Genomic DNA Translation: CAA38745.1
X53769 Genomic DNA Translation: CAJ14141.1
S78982 Genomic DNA Translation: AAB34897.1
AY695821 Genomic DNA Translation: AAU05314.1
AY695822 Genomic DNA Translation: AAU05315.1
CU329671 Genomic DNA Translation: CAA17926.1
PIRiS12605
RefSeqiNP_595292.1, NM_001021199.2

Genome annotation databases

EnsemblFungiiSPBC119.11c.1; SPBC119.11c.1:pep; SPBC119.11c
GeneIDi2540084
KEGGispo:SPBC119.11c

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54998 Genomic DNA Translation: CAA38745.1
X53769 Genomic DNA Translation: CAJ14141.1
S78982 Genomic DNA Translation: AAB34897.1
AY695821 Genomic DNA Translation: AAU05314.1
AY695822 Genomic DNA Translation: AAU05315.1
CU329671 Genomic DNA Translation: CAA17926.1
PIRiS12605
RefSeqiNP_595292.1, NM_001021199.2

3D structure databases

ProteinModelPortaliP22192
SMRiP22192
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276622, 2 interactors
STRINGi4896.SPBC119.11c.1

PTM databases

iPTMnetiP22192

Proteomic databases

MaxQBiP22192
PaxDbiP22192
PRIDEiP22192

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC119.11c.1; SPBC119.11c.1:pep; SPBC119.11c
GeneIDi2540084
KEGGispo:SPBC119.11c

Organism-specific databases

EuPathDBiFungiDB:SPBC119.11c
PomBaseiSPBC119.11c pac1

Phylogenomic databases

InParanoidiP22192
KOiK03685
OMAiWGANQKD
OrthoDBiEOG092C2YRH
PhylomeDBiP22192

Miscellaneous databases

PROiPR:P22192

Family and domain databases

CDDicd00048 DSRM, 1 hit
cd00593 RIBOc, 1 hit
Gene3Di1.10.1520.10, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF00636 Ribonuclease_3, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00535 RIBOc, 1 hit
SUPFAMiSSF69065 SSF69065, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAC1_SCHPO
AccessioniPrimary (citable) accession number: P22192
Secondary accession number(s): Q4JFF8, Q670L9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
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Main funding by: National Institutes of Health

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