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Protein

Iron-regulated transcriptional activator AFT1

Gene

AFT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcription factor that activates the genes for FRE1, FRE2 and FET3 in response to iron deprivation (PubMed:7720713, PubMed:9200812). Also required for the expression of LSO1 (PubMed:26450372). Iron could interact directly with AFT1 and inhibits its activity (PubMed:7720713).3 Publications

Miscellaneous

Present with 2730 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cardiolipin metabolic process Source: SGD
  • chromosome segregation Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • meiotic chromosome segregation Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of iron ion transport Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30574-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-regulated transcriptional activator AFT1
Alternative name(s):
Activator of iron transcription protein 1
Gene namesi
Name:AFT1
Synonyms:RCS1
Ordered Locus Names:YGL071W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL071W
SGDiS000003039 AFT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Two-fold reduction in expression of LSO1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644871 – 690Iron-regulated transcriptional activator AFT1Add BLAST690

Proteomic databases

MaxQBiP22149
PaxDbiP22149
PRIDEiP22149

PTM databases

iPTMnetiP22149

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33177, 306 interactors
DIPiDIP-1351N
IntActiP22149, 63 interactors
MINTiP22149
STRINGi4932.YGL071W

Structurei

3D structure databases

ProteinModelPortaliP22149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00530000069471
InParanoidiP22149
OrthoDBiEOG092C52YQ

Family and domain databases

InterProiView protein in InterPro
IPR014842 AFT
PfamiView protein in Pfam
PF08731 AFT, 1 hit

Sequencei

Sequence statusi: Complete.

P22149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGFNPADIE HASPINSSDS HSSSFVYALP KSASEYVVNH NEGRASASGN
60 70 80 90 100
PAAVPSPIMT LNLKSTHSLN IDQHVHTSTS PTETIGHIHH VEKLNQNNLI
110 120 130 140 150
HLDPVPNFED KSDIKPWLQK IFYPQGIELV IERSDAFKVV FKCKAAKRGR
160 170 180 190 200
NARRKRKDKP KGQDHEDEKS KINDDELEYA SPSNATVTNG PQTSPDQTSS
210 220 230 240 250
IKPKKKRCVS RFNNCPFRVR ATYSLKRKRW SIVVMDNNHS HQLKFNPDSE
260 270 280 290 300
EYKKFKEKLR KDNDVDAIKK FDELEYRTLA NLPIPTATIP CDCGLTNEIQ
310 320 330 340 350
SFNVVLPTNS NVTSSASSST VSSISLDSSN ASKRPCLPSV NNTGSINTNN
360 370 380 390 400
VRKPKSQCKN KDTLLKRTTM QNFLTTKSRL RKTGTPTSSQ HSSTAFSGYI
410 420 430 440 450
DDPFNLNEIL PLPASDFKLN TVTNLNEIDF TNIFTKSPHP HSGSTHPRQV
460 470 480 490 500
FDQLDDCSSI LFSPLTTNTN NEFEGESDDF VHSPYLNSEA DFSQILSSAP
510 520 530 540 550
PVHHDPNETH QENQDIIDRF ANSSQEHNEY ILQYLTHSDA ANHNNIGVPN
560 570 580 590 600
NNSHSLNTQH NVSDLGNSLL RQEALVGSSS TKIFDELKFV QNGPHGSQHP
610 620 630 640 650
IDFQHVDHRH LSSNEPQVRS HQYGPQQQPP QQLQYHQNQP HDGHNHEQHQ
660 670 680 690
TVQKDMQTHE SLEIMGNTLL EEFKDIKMVN GELKYVKPED
Length:690
Mass (Da):77,683
Last modified:November 1, 1995 - v2
Checksum:i38641F26B76FCBDD
GO

Sequence cautioni

The sequence CAA37215 differs from that shown. Reason: Frameshift at positions 604 and 644.Curated
The sequence CAA54586 differs from that shown. Reason: Frameshift at positions 604 and 644.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8D → H in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti71I → S in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti136A → T in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti236D → N in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti329S → L in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti378S → L in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti416D → G in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti416D → G in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti468N → T in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti507N → S in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti507N → S in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti538S → F in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti538S → L in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti568S → P in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti568S → P in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti579S → T in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti579S → T in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti644H → I in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti644H → I in CAA37215 (PubMed:2021081).Curated1
Sequence conflicti649H → L in CAA54586 (PubMed:9200812).Curated1
Sequence conflicti649H → L in CAA37215 (PubMed:2021081).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti291C → F in allele AFT1-1UP; which is constitutively activated. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48004 mRNA Translation: CAA88044.1
X77413 Genomic DNA Translation: CAA54586.1 Frameshift.
Z72593 Genomic DNA Translation: CAA96775.1
AY723805 Genomic DNA Translation: AAU09722.1
X53046 Genomic DNA Translation: CAA37215.1 Frameshift.
BK006941 Genomic DNA Translation: DAA08032.1
PIRiS54775
RefSeqiNP_011444.1, NM_001180936.1

Genome annotation databases

EnsemblFungiiYGL071W; YGL071W; YGL071W
GeneIDi852809
KEGGisce:YGL071W

Similar proteinsi

Entry informationi

Entry nameiAFT1_YEAST
AccessioniPrimary (citable) accession number: P22149
Secondary accession number(s): D6VU71, Q06993
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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