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Protein

NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

Gene

nuo-49

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi341Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi347Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi362Iron-sulfur (4Fe-4S)Sequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, Translocase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial (EC:1.6.99.3, EC:7.1.1.2)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
Gene namesi
Name:nuo-49
ORF Names:NCU02534
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 1, Linkage Group I

Organism-specific databases

EuPathDBiFungiDB:NCU02534

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42Mitochondrion1 PublicationAdd BLAST42
ChainiPRO_000001998443 – 478NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrialAdd BLAST436

Proteomic databases

PRIDEiP22142

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits.

Structurei

3D structure databases

ProteinModelPortaliP22142
SMRiP22142
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000228264
InParanoidiP22142
KOiK03935
OrthoDBiEOG092C1XVX

Family and domain databases

Gene3Di1.10.645.20, 1 hit
HAMAPiMF_01358 NDH1_NuoD, 1 hit
InterProiView protein in InterPro
IPR001135 NADH_Q_OxRdtase_suD
IPR038290 NADH_Q_OxRdtase_suD_sf
IPR014029 NADH_UbQ_OxRdtase_49kDa_CS
IPR022885 NDH1_su_D/H
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00346 Complex1_49kDa, 1 hit
SUPFAMiSSF56762 SSF56762, 1 hit
TIGRFAMsiTIGR01962 NuoD, 1 hit
PROSITEiView protein in PROSITE
PS00535 COMPLEX1_49K, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22142-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATTLFRLAG RNAKRHCMRQ STTIAHNLNS TRAFSASALR RYAEPSYEGQ
60 70 80 90 100
GTRLVPTGDD FAPNNDLYGL EALKADGAPR VPPQDHILAR KVRHYTVNFG
110 120 130 140 150
PQHPAAHGVL RLILELKGEE IVRADPHVGL LHRGTEKLCE YRTYLQALPY
160 170 180 190 200
FDRLDYVSMM TNEQCFALAV EKLLNVEIPE RAKWIRTMFA EITRILNHLM
210 220 230 240 250
SVLSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVHQD
260 270 280 290 300
IPLGLLDDIY MWATQFGDRI DETEEMLTDN RIWIDRLRGI GVVSAADALN
310 320 330 340 350
LSFTGVMLRG SGVPWDIRKS QPYDAYDQVE FDVPVGINGD CYDRYLCRME
360 370 380 390 400
EFRQSLRIIH QCLNKMPAGP VRVEDYKISP PPRSAMKENM EALIHHFLLY
410 420 430 440 450
TKGYAVPPGD TYSAIEAPKG EMGVYVVSDG SERPYRVHIR APGFAHLGGF
460 470
DHLSRGHMLA DAVAVIGTMD LVFGEVDR
Length:478
Mass (Da):54,019
Last modified:December 4, 2007 - v2
Checksum:i159C35BE3DF9D916
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti417A → G in CAA38368 (PubMed:2147127).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54508 Genomic DNA Translation: CAA38368.1
CM002236 Genomic DNA Translation: EAA36429.2
PIRiS13801
RefSeqiXP_965665.2, XM_960572.3

Genome annotation databases

EnsemblFungiiEAA36429; EAA36429; NCU02534
GeneIDi3881799
KEGGincr:NCU02534

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54508 Genomic DNA Translation: CAA38368.1
CM002236 Genomic DNA Translation: EAA36429.2
PIRiS13801
RefSeqiXP_965665.2, XM_960572.3

3D structure databases

ProteinModelPortaliP22142
SMRiP22142
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.D.1.6.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Proteomic databases

PRIDEiP22142

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA36429; EAA36429; NCU02534
GeneIDi3881799
KEGGincr:NCU02534

Organism-specific databases

EuPathDBiFungiDB:NCU02534

Phylogenomic databases

HOGENOMiHOG000228264
InParanoidiP22142
KOiK03935
OrthoDBiEOG092C1XVX

Family and domain databases

Gene3Di1.10.645.20, 1 hit
HAMAPiMF_01358 NDH1_NuoD, 1 hit
InterProiView protein in InterPro
IPR001135 NADH_Q_OxRdtase_suD
IPR038290 NADH_Q_OxRdtase_suD_sf
IPR014029 NADH_UbQ_OxRdtase_49kDa_CS
IPR022885 NDH1_su_D/H
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00346 Complex1_49kDa, 1 hit
SUPFAMiSSF56762 SSF56762, 1 hit
TIGRFAMsiTIGR01962 NuoD, 1 hit
PROSITEiView protein in PROSITE
PS00535 COMPLEX1_49K, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS2_NEUCR
AccessioniPrimary (citable) accession number: P22142
Secondary accession number(s): Q7RVM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 4, 2007
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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