UniProtKB - P22083 (FUT4_HUMAN)
Alpha-(1,3)-fucosyltransferase 4
FUT4
Functioni
Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed:29593094, PubMed:1702034, PubMed:1716630).
Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed:29593094, PubMed:1716630).
Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity).
In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:1716630, PubMed:11278338).
By similarity3 PublicationsDoes not generate Lewis X antigens.
1 PublicationCatalytic activityi
- a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + GDP-β-L-fucose = a β-D-galactosyl-(1→4)-[α-L-fucosyl-(1→3)]-N-acetyl-β-D-glucosaminyl derivative + GDP + H+2 PublicationsEC:2.4.1.1522 PublicationsThis reaction proceeds in the forward1 Publication direction.
- an N-acetyl-α-neuraminyl-(2→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + GDP-β-L-fucose = an α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-[α-L-Fuc-(1→3)]-β-D-GlcNAc derivative + GDP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.This reaction proceeds in the forward1 Publication direction.
- an α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-β-D-GlcNAc derivative + GDP-β-L-fucose = an α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-[α-L-Fuc-(1→3)]-β-D-GlcNAc derivative + GDP + H+2 PublicationsThis reaction proceeds in the forward2 Publications direction.This reaction proceeds in the forward2 Publications direction.
- an α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-GlcNAc6S derivative + GDP-β-L-fucose = an α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-[α-L-Fuc-(1→3)]-β-D-GlcNAc6S derivative + GDP + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity Source: UniProtKB
- alpha-(1->3)-fucosyltransferase activity Source: GO_Central
- fucosyltransferase activity Source: ProtInc
GO - Biological processi
- carbohydrate metabolic process Source: ProtInc
- fucosylation Source: GO_Central
- glycosphingolipid biosynthetic process Source: UniProtKB
- L-fucose catabolic process Source: UniProtKB
- lymphocyte migration into lymph node Source: UniProtKB
- oligosaccharide biosynthetic process Source: Reactome
- oligosaccharide metabolic process Source: UniProtKB
- positive regulation of leukocyte tethering or rolling Source: UniProtKB
- positive regulation of neutrophil migration Source: UniProtKB
- protein glycosylation Source: UniProtKB
- protein N-linked glycosylation Source: UniProtKB
- protein O-linked glycosylation Source: UniProtKB
- regulation of leukocyte cell-cell adhesion Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Inflammatory response |
Enzyme and pathway databases
BRENDAi | 2.4.1.152, 2681 |
PathwayCommonsi | P22083 |
Reactomei | R-HSA-9037629, Lewis blood group biosynthesis |
SignaLinki | P22083 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT10, Glycosyltransferase Family 10 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-(1,3)-fucosyltransferase 4Alternative name(s): 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (EC:2.4.1.1522 Publications) ELAM-1 ligand fucosyltransferase1 Publication Fucosyltransferase 4 Fucosyltransferase IV Short name: Fuc-TIV Short name: FucT-IV Galactoside 3-L-fucosyltransferase |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4015, FUT4 |
MIMi | 104230, gene |
neXtProti | NX_P22083 |
VEuPathDBi | HostDB:ENSG00000196371 |
Subcellular locationi
Golgi apparatus
Note: Membrane-bound form in trans cisternae of Golgi.
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi membrane Source: Reactome
- trans-Golgi network Source: UniProtKB
Other locations
- cell periphery Source: Ensembl
- cell surface Source: Ensembl
- integral component of membrane Source: UniProtKB-KW
- membrane Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 147 | CytoplasmicSequence analysisAdd BLAST | 147 | |
Transmembranei | 148 – 172 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 25 | |
Topological domaini | 173 – 530 | LumenalSequence analysisAdd BLAST | 358 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 2526 |
OpenTargetsi | ENSG00000196371 |
PharmGKBi | PA28431 |
Miscellaneous databases
Pharosi | P22083, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4996 |
Genetic variation databases
BioMutai | FUT4 |
DMDMi | 226694189 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000221100 | 1 – 530 | Alpha-(1,3)-fucosyltransferase 4Add BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 216 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 315 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | P22083 |
jPOSTi | P22083 |
MassIVEi | P22083 |
MaxQBi | P22083 |
PaxDbi | P22083 |
PeptideAtlasi | P22083 |
PRIDEi | P22083 |
ProteomicsDBi | 53958 |
PTM databases
GlyGeni | P22083, 2 sites |
iPTMneti | P22083 |
PhosphoSitePlusi | P22083 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000196371, Expressed in bone marrow and 181 other tissues |
Genevisiblei | P22083, HS |
Organism-specific databases
HPAi | ENSG00000196371, Tissue enriched (bone) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 108802, 8 interactors |
IntActi | P22083, 2 interactors |
MINTi | P22083 |
STRINGi | 9606.ENSP00000351602 |
Miscellaneous databases
RNActi | P22083, protein |
Structurei
3D structure databases
AlphaFoldDBi | P22083 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | DisorderedSequence analysisAdd BLAST | 48 | |
Regioni | 66 – 112 | DisorderedSequence analysisAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 88 – 102 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2619, Eukaryota |
GeneTreei | ENSGT00940000162506 |
HOGENOMi | CLU_032075_4_0_1 |
InParanoidi | P22083 |
OMAi | ANYERFM |
OrthoDBi | 551308at2759 |
PhylomeDBi | P22083 |
TreeFami | TF316348 |
Family and domain databases
Gene3Di | 3.40.50.11660, 1 hit |
InterProi | View protein in InterPro IPR031481, Glyco_tran_10_N IPR001503, Glyco_trans_10 IPR038577, GT10-like_C_sf |
PANTHERi | PTHR11929, PTHR11929, 1 hit |
Pfami | View protein in Pfam PF17039, Glyco_tran_10_N, 1 hit PF00852, Glyco_transf_10, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRLWGAARK PSGAGWEKEW AEAPQEAPGA WSGRLGPGRS GRKGRAVPGW
60 70 80 90 100
ASWPAHLALA ARPARHLGGA GQGPRPLHSG TAPFHSRASG ERQRRLEPQL
110 120 130 140 150
QHESRCRSST PADAWRAEAA LPVRAMGAPW GSPTAAAGGR RGWRRGRGLP
160 170 180 190 200
WTVCVLAAAG LTCTALITYA CWGQLPPLPW ASPTPSRPVG VLLWWEPFGG
210 220 230 240 250
RDSAPRPPPD CRLRFNISGC RLLTDRASYG EAQAVLFHHR DLVKGPPDWP
260 270 280 290 300
PPWGIQAHTA EEVDLRVLDY EEAAAAAEAL ATSSPRPPGQ RWVWMNFESP
310 320 330 340 350
SHSPGLRSLA SNLFNWTLSY RADSDVFVPY GYLYPRSHPG DPPSGLAPPL
360 370 380 390 400
SRKQGLVAWV VSHWDERQAR VRYYHQLSQH VTVDVFGRGG PGQPVPEIGL
410 420 430 440 450
LHTVARYKFY LAFENSQHLD YITEKLWRNA LLAGAVPVVL GPDRANYERF
460 470 480 490 500
VPRGAFIHVD DFPSASSLAS YLLFLDRNPA VYRRYFHWRR SYAVHITSFW
510 520 530
DEPWCRVCQA VQRAGDRPKS IRNLASWFER
The sequence of this isoform differs from the canonical sequence as follows:
1-125: Missing.
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 212 | R → P in AAA92977 (PubMed:1716630).Curated | 1 | |
Sequence conflicti | 366 | E → D in AAB20349 (PubMed:1718983).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055844 | 255 | I → V. Corresponds to variant dbSNP:rs2230273Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_061247 | 1 – 125 | Missing in isoform Short. 1 PublicationAdd BLAST | 125 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58596 mRNA Translation: AAA63172.1 M58597 mRNA Translation: AAA63173.1 M65030 Genomic DNA Translation: AAA92977.1 AP000943 Genomic DNA No translation available. CH471065 Genomic DNA Translation: EAW66938.1 BC136373 mRNA Translation: AAI36374.1 BC136374 mRNA Translation: AAI36375.1 S65161 Genomic DNA Translation: AAB20349.1 Sequence problems. |
CCDSi | CCDS8301.1 [P22083-1] |
PIRi | B36340 |
RefSeqi | NP_002024.1, NM_002033.3 |
Genome annotation databases
Ensembli | ENST00000358752.4; ENSP00000351602.2; ENSG00000196371.4 |
GeneIDi | 2526 |
KEGGi | hsa:2526 |
MANE-Selecti | ENST00000358752.4; ENSP00000351602.2; NM_002033.4; NP_002024.1 |
UCSCi | uc001pez.4, human [P22083-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Fucosyltransferase 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58596 mRNA Translation: AAA63172.1 M58597 mRNA Translation: AAA63173.1 M65030 Genomic DNA Translation: AAA92977.1 AP000943 Genomic DNA No translation available. CH471065 Genomic DNA Translation: EAW66938.1 BC136373 mRNA Translation: AAI36374.1 BC136374 mRNA Translation: AAI36375.1 S65161 Genomic DNA Translation: AAB20349.1 Sequence problems. |
CCDSi | CCDS8301.1 [P22083-1] |
PIRi | B36340 |
RefSeqi | NP_002024.1, NM_002033.3 |
3D structure databases
AlphaFoldDBi | P22083 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 108802, 8 interactors |
IntActi | P22083, 2 interactors |
MINTi | P22083 |
STRINGi | 9606.ENSP00000351602 |
Chemistry databases
ChEMBLi | CHEMBL4996 |
Protein family/group databases
CAZyi | GT10, Glycosyltransferase Family 10 |
PTM databases
GlyGeni | P22083, 2 sites |
iPTMneti | P22083 |
PhosphoSitePlusi | P22083 |
Genetic variation databases
BioMutai | FUT4 |
DMDMi | 226694189 |
Proteomic databases
EPDi | P22083 |
jPOSTi | P22083 |
MassIVEi | P22083 |
MaxQBi | P22083 |
PaxDbi | P22083 |
PeptideAtlasi | P22083 |
PRIDEi | P22083 |
ProteomicsDBi | 53958 |
Protocols and materials databases
Antibodypediai | 3496, 1968 antibodies from 46 providers |
DNASUi | 2526 |
Genome annotation databases
Ensembli | ENST00000358752.4; ENSP00000351602.2; ENSG00000196371.4 |
GeneIDi | 2526 |
KEGGi | hsa:2526 |
MANE-Selecti | ENST00000358752.4; ENSP00000351602.2; NM_002033.4; NP_002024.1 |
UCSCi | uc001pez.4, human [P22083-1] |
Organism-specific databases
CTDi | 2526 |
DisGeNETi | 2526 |
GeneCardsi | FUT4 |
HGNCi | HGNC:4015, FUT4 |
HPAi | ENSG00000196371, Tissue enriched (bone) |
MIMi | 104230, gene |
neXtProti | NX_P22083 |
OpenTargetsi | ENSG00000196371 |
PharmGKBi | PA28431 |
VEuPathDBi | HostDB:ENSG00000196371 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2619, Eukaryota |
GeneTreei | ENSGT00940000162506 |
HOGENOMi | CLU_032075_4_0_1 |
InParanoidi | P22083 |
OMAi | ANYERFM |
OrthoDBi | 551308at2759 |
PhylomeDBi | P22083 |
TreeFami | TF316348 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.152, 2681 |
PathwayCommonsi | P22083 |
Reactomei | R-HSA-9037629, Lewis blood group biosynthesis |
SignaLinki | P22083 |
Miscellaneous databases
BioGRID-ORCSi | 2526, 24 hits in 1091 CRISPR screens |
GenomeRNAii | 2526 |
Pharosi | P22083, Tbio |
PROi | PR:P22083 |
RNActi | P22083, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196371, Expressed in bone marrow and 181 other tissues |
Genevisiblei | P22083, HS |
Family and domain databases
Gene3Di | 3.40.50.11660, 1 hit |
InterProi | View protein in InterPro IPR031481, Glyco_tran_10_N IPR001503, Glyco_trans_10 IPR038577, GT10-like_C_sf |
PANTHERi | PTHR11929, PTHR11929, 1 hit |
Pfami | View protein in Pfam PF17039, Glyco_tran_10_N, 1 hit PF00852, Glyco_transf_10, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FUT4_HUMAN | |
Accessioni | P22083Primary (citable) accession number: P22083 Secondary accession number(s): B2RMS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | April 14, 2009 | |
Last modified: | May 25, 2022 | |
This is version 181 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families