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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Gene

Hsd3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Caution

Rat 3-beta-HSD type II may possess only one transmembrane domain.Curated

Catalytic activityi

A 3-beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH.
A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Oxidoreductase
Biological processSteroidogenesis
LigandNAD

Enzyme and pathway databases

UniPathwayi
UPA00062

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name:
3-beta-HSD II
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1592771 Hsd3b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877881 – 3733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2Add BLAST373

PTM databases

iPTMnetiP22072
PhosphoSitePlusiP22072

Expressioni

Tissue specificityi

Adrenal glands, testes and ovaries.

Structurei

3D structure databases

ProteinModelPortaliP22072
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiP22072
KOiK00070

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

P22072-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKAKVTMLE GDILDAQYLR RACQGISVVI HTASVMDFSR VLPRQTILDV
110 120 130 140 150
NLKGTQNLLE AGIHASVPAF IYCSTVDVAG PNSYKKTILN GREEEHHEST
160 170 180 190 200
WSNPYPYSKK MAEKAVLAAN GSILKNGGTL HTCALRPMYI YGERGQFLSR
210 220 230 240 250
IIIMALKNKG VLNVTGKFSI VNPVYVGNVA WAHILAARGL RDPKKSQNIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):42,277
Last modified:January 23, 2007 - v3
Checksum:i0F922E9632DE9526
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1LNS3F1LNS3_RAT
Hydroxy-delta-5-steroid dehydrogena...
Hsd3b3
373Annotation score:
A0A140TAJ4A0A140TAJ4_RAT
Hydroxy-delta-5-steroid dehydrogena...
Hsd3b3
373Annotation score:
A0A0G2KAA4A0A0G2KAA4_RAT
Hydroxy-delta-5-steroid dehydrogena...
Hsd3b3
354Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115A → T in AAB20228 (PubMed:1944305).Curated1
Sequence conflicti165A → S in AAB20228 (PubMed:1944305).Curated1
Sequence conflicti341E → V in AAB20228 (PubMed:1944305).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38179 mRNA Translation: AAA63475.1
S63167 mRNA Translation: AAB20228.1
PIRiA40378 DERTHM
B39051 DERTH2
RefSeqiNP_001036084.1, NM_001042619.1
UniGeneiRn.128814

Genome annotation databases

GeneIDi682974
KEGGirno:682974
UCSCiRGD:1592771 rat

Similar proteinsi

Entry informationi

Entry namei3BHS2_RAT
AccessioniPrimary (citable) accession number: P22072
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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