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UniProtKB - P22072 (3BHS2_RAT)
Protein
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Gene
Hsd3b
Organism
Rattus norvegicus (Rat)
Status
Functioni
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Caution
Rat 3-beta-HSD type II may possess only one transmembrane domain.Curated
Catalytic activityi
: steroid biosynthesis Pathwayi
This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 155 | Proton acceptorBy similarity | 1 | |
Binding sitei | 159 | NADBy similarity | 1 |
GO - Molecular functioni
- 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: UniProtKB-EC
- cholesterol dehydrogenase activity Source: UniProtKB-EC
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: GO_Central
- steroid delta-isomerase activity Source: UniProtKB-EC
GO - Biological processi
- C21-steroid hormone metabolic process Source: GO_Central
- hippocampus development Source: GO_Central
- response to corticosterone Source: GO_Central
- steroid biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Isomerase, Multifunctional enzyme, Oxidoreductase |
Biological process | Steroidogenesis |
Ligand | NAD |
Enzyme and pathway databases
UniPathwayi | UPA00062 |
Names & Taxonomyi
Protein namesi | Recommended name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2Alternative name(s): 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II Short name: 3-beta-HSD II Including the following 2 domains: |
Gene namesi | Name:Hsd3b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1592771, Hsd3b |
Subcellular locationi
Mitochondrion
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 288 – 308 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087788 | 1 – 373 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2Add BLAST | 373 |
PTM databases
iPTMneti | P22072 |
PhosphoSitePlusi | P22072 |
Expressioni
Tissue specificityi
Adrenal glands, testes and ovaries.
Family & Domainsi
Sequence similaritiesi
Belongs to the 3-beta-HSD family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | P22072 |
OrthoDBi | 930591at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR002225, 3Beta_OHSteriod_DH/Estase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01073, 3Beta_HSD, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P22072-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKAKVTMLE GDILDAQYLR RACQGISVVI HTASVMDFSR VLPRQTILDV
110 120 130 140 150
NLKGTQNLLE AGIHASVPAF IYCSTVDVAG PNSYKKTILN GREEEHHEST
160 170 180 190 200
WSNPYPYSKK MAEKAVLAAN GSILKNGGTL HTCALRPMYI YGERGQFLSR
210 220 230 240 250
IIIMALKNKG VLNVTGKFSI VNPVYVGNVA WAHILAARGL RDPKKSQNIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LNS3 | F1LNS3_RAT | Hydroxy-delta-5-steroid dehydrogena... | Hsd3b3 | 373 | Annotation score: | ||
A0A0G2KAA4 | A0A0G2KAA4_RAT | Hydroxy-delta-5-steroid dehydrogena... | Hsd3b3 | 354 | Annotation score: | ||
A0A140TAJ4 | A0A140TAJ4_RAT | Hydroxy-delta-5-steroid dehydrogena... | Hsd3b3 | 373 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 115 | A → T in AAB20228 (PubMed:1944305).Curated | 1 | |
Sequence conflicti | 165 | A → S in AAB20228 (PubMed:1944305).Curated | 1 | |
Sequence conflicti | 341 | E → V in AAB20228 (PubMed:1944305).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M38179 mRNA Translation: AAA63475.1 S63167 mRNA Translation: AAB20228.1 |
PIRi | A40378, DERTHM B39051, DERTH2 |
RefSeqi | NP_001036084.1, NM_001042619.1 |
Genome annotation databases
GeneIDi | 682974 |
KEGGi | rno:682974 |
UCSCi | RGD:1592771, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M38179 mRNA Translation: AAA63475.1 S63167 mRNA Translation: AAB20228.1 |
PIRi | A40378, DERTHM B39051, DERTH2 |
RefSeqi | NP_001036084.1, NM_001042619.1 |
3D structure databases
SMRi | P22072 |
ModBasei | Search... |
PTM databases
iPTMneti | P22072 |
PhosphoSitePlusi | P22072 |
Genome annotation databases
GeneIDi | 682974 |
KEGGi | rno:682974 |
UCSCi | RGD:1592771, rat |
Organism-specific databases
CTDi | 15494 |
RGDi | 1592771, Hsd3b |
Phylogenomic databases
InParanoidi | P22072 |
OrthoDBi | 930591at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00062 |
Miscellaneous databases
PROi | PR:P22072 |
Family and domain databases
InterProi | View protein in InterPro IPR002225, 3Beta_OHSteriod_DH/Estase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01073, 3Beta_HSD, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 3BHS2_RAT | |
Accessioni | P22072Primary (citable) accession number: P22072 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families