Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proenkephalin-A

Gene

Penk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndorphin, Neuropeptide, Opioid peptide

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-418594 G alpha (i) signalling events
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Proenkephalin-A
Cleaved into the following 8 chains:
Gene namesi
Name:Penk
Synonyms:Penk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:104629 Penk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
PeptideiPRO_000000826825 – 97SynenkephalinAdd BLAST73
PeptideiPRO_0000008269100 – 104Met-enkephalin5
PeptideiPRO_0000008270107 – 111Met-enkephalin5
PeptideiPRO_0000377694114 – 133PENK(114-133)By similarityAdd BLAST20
PeptideiPRO_0000008272136 – 140Met-enkephalin5
PeptideiPRO_0000377695143 – 184PENK(143-184)By similarityAdd BLAST42
PeptideiPRO_0000008274187 – 194Met-enkephalin-Arg-Ser-Leu8
PropeptideiPRO_0000008275197 – 208Add BLAST12
PeptideiPRO_0000008276211 – 215Met-enkephalin5
PropeptideiPRO_0000008277218 – 228Add BLAST11
PeptideiPRO_0000008278231 – 235Leu-enkephalin5
PeptideiPRO_0000377696238 – 259PENK(238-259)By similarityAdd BLAST22
PeptideiPRO_0000008280262 – 268Met-enkephalin-Arg-Phe7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 48By similarity
Disulfide bondi30 ↔ 52By similarity
Disulfide bondi33 ↔ 65By similarity
Modified residuei252PhosphoserineBy similarity1

Post-translational modificationi

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP22005
PaxDbiP22005
PeptideAtlasiP22005
PRIDEiP22005

PTM databases

PhosphoSitePlusiP22005

Expressioni

Tissue specificityi

Spermatogenic and somatic cells.

Developmental stagei

Highest expression in late pachytene spermatocytes and postmeiotic round spermatids.

Gene expression databases

BgeeiENSMUSG00000045573
CleanExiMM_PENK1
ExpressionAtlasiP22005 baseline and differential
GenevisibleiP22005 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202109, 1 interactor
IntActiP22005, 1 interactor
MINTiP22005
STRINGi10090.ENSMUSP00000066822

Structurei

3D structure databases

ProteinModelPortaliP22005
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJUY Eukaryota
ENOG4110ZYD LUCA
GeneTreeiENSGT00530000063761
HOGENOMiHOG000013003
HOVERGENiHBG000063
InParanoidiP22005
KOiK18832
OMAiGFMKKMD
OrthoDBiEOG091G0HV8
PhylomeDBiP22005
TreeFamiTF332620

Family and domain databases

InterProiView protein in InterPro
IPR006024 Opioid_neupept
IPR000703 Proenkphlin_A
PANTHERiPTHR11438 PTHR11438, 1 hit
PTHR11438:SF3 PTHR11438:SF3, 1 hit
PfamiView protein in Pfam
PF01160 Opiods_neuropep, 1 hit
PRINTSiPR01028 OPIOIDPRCRSR
PR01029 PENKAPRCRSR
PROSITEiView protein in PROSITE
PS01252 OPIOIDS_PRECURSOR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFLRLCTW LLALGSCLLA TVQAECSQDC AKCSYRLVRP GDINFLACTL
60 70 80 90 100
ECEGQLPSFK IWETCKDLLQ VSRPEFPWDN IDMYKDSSKQ DESHLLAKKY
110 120 130 140 150
GGFMKRYGGF MKKMDELYPM EPEEEANGGE ILAKRYGGFM KKDADEGDTL
160 170 180 190 200
ANSSDLLKEL LGTGDNRAKD SHQQESTNND EDMSKRYGGF MRSLKRSPQL
210 220 230 240 250
EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE SLPSDEEGEN
260
YSKEVPEIEK RYGGFMRF
Length:268
Mass (Da):31,004
Last modified:June 16, 2009 - v2
Checksum:i637F848B49F44013
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34S → T in AAA40128 (PubMed:2355920).Curated1
Sequence conflicti184S → SS in AAA40128 (PubMed:2355920).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55181 mRNA Translation: AAA40128.1
AK161272 mRNA Translation: BAE36283.1
BX004841 Genomic DNA Translation: CAM26883.1
CH466538 Genomic DNA Translation: EDL05699.1
CH466538 Genomic DNA Translation: EDL05700.1
BC049766 mRNA Translation: AAH49766.1
M13227 mRNA Translation: AAA37553.1
CCDSiCCDS17946.1
PIRiB35678
RefSeqiNP_001002927.1, NM_001002927.3
NP_001335138.1, NM_001348209.1
UniGeneiMm.475097

Genome annotation databases

EnsembliENSMUST00000070375; ENSMUSP00000066822; ENSMUSG00000045573
GeneIDi18619
KEGGimmu:18619
UCSCiuc008rwz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPENK_MOUSE
AccessioniPrimary (citable) accession number: P22005
Secondary accession number(s): Q68G73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 16, 2009
Last modified: June 20, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health