UniProtKB - P22001 (KCNA3_HUMAN)
Protein
Potassium voltage-gated channel subfamily A member 3
Gene
KCNA3
Organism
Homo sapiens (Human)
Status
Functioni
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Caution
It is uncertain whether Met-1 or Met-53 is the initiator.Curated
GO - Molecular functioni
- delayed rectifier potassium channel activity Source: GO_Central
- outward rectifier potassium channel activity Source: Ensembl
- voltage-gated ion channel activity Source: ProtInc
- voltage-gated potassium channel activity Source: GO_Central
GO - Biological processi
- potassium ion transmembrane transport Source: GO_Central
- potassium ion transport Source: ProtInc
- protein homooligomerization Source: InterPro
- regulation of ion transmembrane transport Source: UniProtKB-KW
Keywordsi
Molecular function | Ion channel, Potassium channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
PathwayCommonsi | P22001 |
Reactomei | R-HSA-1296072, Voltage gated Potassium channels |
SIGNORi | P22001 |
Protein family/group databases
TCDBi | 1.A.1.2.4, the voltage-gated ion channel (vic) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium voltage-gated channel subfamily A member 3Alternative name(s): HGK5 HLK3 HPCN3 Voltage-gated K(+) channel HuKIII Voltage-gated potassium channel subunit Kv1.3 |
Gene namesi | Name:KCNA3 Synonyms:HGK5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6221, KCNA3 |
MIMi | 176263, gene |
neXtProti | NX_P22001 |
VEuPathDBi | HostDB:ENSG00000177272.8 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein
Plasma Membrane
- integral component of postsynaptic membrane Source: Ensembl
- integral component of presynaptic membrane Source: Ensembl
- plasma membrane Source: Reactome
- voltage-gated potassium channel complex Source: GO_Central
Other locations
- axon Source: GO_Central
- calyx of Held Source: Ensembl
- glutamatergic synapse Source: Ensembl
- integral component of membrane Source: GO_Central
- membrane raft Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 234 | CytoplasmicSequence analysisAdd BLAST | 234 | |
Transmembranei | 235 – 253 | Helical; Name=Segment S1Sequence analysisAdd BLAST | 19 | |
Topological domaini | 254 – 294 | ExtracellularSequence analysisAdd BLAST | 41 | |
Transmembranei | 295 – 316 | Helical; Name=Segment S2Sequence analysisAdd BLAST | 22 | |
Topological domaini | 317 – 327 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 328 – 348 | Helical; Name=Segment S3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 349 – 362 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 363 – 381 | Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST | 19 | |
Topological domaini | 382 – 397 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 398 – 417 | Helical; Name=Segment S5Sequence analysisAdd BLAST | 20 | |
Topological domaini | 418 – 458 | ExtracellularSequence analysisAdd BLAST | 41 | |
Transmembranei | 459 – 481 | Helical; Name=Segment S6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 482 – 575 | CytoplasmicSequence analysisAdd BLAST | 94 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 3738 |
OpenTargetsi | ENSG00000177272 |
PharmGKBi | PA30021 |
Miscellaneous databases
Pharosi | P22001, Tclin |
Chemistry databases
ChEMBLi | CHEMBL4633 |
DrugBanki | DB00845, Clofazimine DB06637, Dalfampridine DB00228, Enflurane DB01110, Miconazole DB01069, Promethazine |
DrugCentrali | P22001 |
GuidetoPHARMACOLOGYi | 540 |
Genetic variation databases
BioMutai | KCNA3 |
DMDMi | 215274135 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053977 | 1 – 575 | Potassium voltage-gated channel subfamily A member 3Add BLAST | 575 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 279 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 317 | S-palmitoyl cysteineSequence analysis | 1 | |
Modified residuei | 520 | Phosphoserine; by PKASequence analysis | 1 |
Post-translational modificationi
N-glycosylation promotes the cell surface expression.By similarity
Keywords - PTMi
Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
jPOSTi | P22001 |
MassIVEi | P22001 |
MaxQBi | P22001 |
PaxDbi | P22001 |
PeptideAtlasi | P22001 |
PRIDEi | P22001 |
ProteomicsDBi | 53950 |
PTM databases
GlyGeni | P22001, 1 site |
iPTMneti | P22001 |
PhosphoSitePlusi | P22001 |
Expressioni
Gene expression databases
Bgeei | ENSG00000177272, Expressed in leukocyte and 107 other tissues |
Genevisiblei | P22001, HS |
Organism-specific databases
HPAi | ENSG00000177272, Tissue enriched (lymphoid) |
Interactioni
Subunit structurei
Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
By similarityBinary interactionsi
Hide detailsP22001
With | #Exp. | IntAct |
---|---|---|
BAX [Q07812] | 2 | EBI-8627664,EBI-516580 |
DOLK [Q9UPQ8] | 6 | EBI-8627664,EBI-8645574 |
KCNA1 [Q09470] | 3 | EBI-8627664,EBI-8286599 |
Protein-protein interaction databases
BioGRIDi | 109941, 25 interactors |
DIPi | DIP-44822N |
IntActi | P22001, 5 interactors |
MINTi | P22001 |
STRINGi | 9606.ENSP00000358784 |
Chemistry databases
BindingDBi | P22001 |
Miscellaneous databases
RNActi | P22001, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P22001 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 444 – 449 | Selectivity filterBy similarity | 6 | |
Motifi | 573 – 575 | PDZ-bindingBy similarity | 3 |
Domaini
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Sequence similaritiesi
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1545, Eukaryota |
GeneTreei | ENSGT00940000160210 |
HOGENOMi | CLU_011722_4_0_1 |
InParanoidi | P22001 |
OMAi | TTNNPNC |
OrthoDBi | 695337at2759 |
PhylomeDBi | P22001 |
TreeFami | TF313103 |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003972, K_chnl_volt-dep_Kv1 IPR004050, K_chnl_volt-dep_Kv1.3 IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF00520, Ion_trans, 1 hit |
PRINTSi | PR01510, KV13CHANNEL PR01491, KVCHANNEL PR01496, SHAKERCHANEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
i Sequence
Sequence statusi: Complete.
P22001-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDERLSLLRS PPPPSARHRA HPPQRPASSG GAHTLVNHGY AEPAAGRELP
60 70 80 90 100
PDMTVVPGDH LLEPEVADGG GAPPQGGCGG GGCDRYEPLP PSLPAAGEQD
110 120 130 140 150
CCGERVVINI SGLRFETQLK TLCQFPETLL GDPKRRMRYF DPLRNEYFFD
160 170 180 190 200
RNRPSFDAIL YYYQSGGRIR RPVNVPIDIF SEEIRFYQLG EEAMEKFRED
210 220 230 240 250
EGFLREEERP LPRRDFQRQV WLLFEYPESS GPARGIAIVS VLVILISIVI
260 270 280 290 300
FCLETLPEFR DEKDYPASTS QDSFEAAGNS TSGSRAGASS FSDPFFVVET
310 320 330 340 350
LCIIWFSFEL LVRFFACPSK ATFSRNIMNL IDIVAIIPYF ITLGTELAER
360 370 380 390 400
QGNGQQAMSL AILRVIRLVR VFRIFKLSRH SKGLQILGQT LKASMRELGL
410 420 430 440 450
LIFFLFIGVI LFSSAVYFAE ADDPTSGFSS IPDAFWWAVV TMTTVGYGDM
460 470 480 490 500
HPVTIGGKIV GSLCAIAGVL TIALPVPVIV SNFNYFYHRE TEGEEQSQYM
510 520 530 540 550
HVGSCQHLSS SAEELRKARS NSTLSKSEYM VIEEGGMNHS AFPQTPFKTG
560 570
NSTATCTTNN NPNSCVNIKK IFTDV
Sequence cautioni
The sequence AAA36425 differs from that shown. Reason: Frameshift.Curated
The sequence AAA36425 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAA59457 differs from that shown. Reason: Frameshift.Curated
The sequence AAA59457 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAB88073 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAC31761 differs from that shown. Reason: Frameshift.Curated
The sequence AAC31761 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | A → G in AAA36425 (PubMed:1986382).Curated | 1 | |
Sequence conflicti | 89 | L → V in AAA36425 (PubMed:1986382).Curated | 1 | |
Sequence conflicti | 113 | L → V in AAA59457 (PubMed:1373731).Curated | 1 | |
Sequence conflicti | 143 | L → V in AAA36425 (PubMed:1986382).Curated | 1 | |
Sequence conflicti | 309 | E → K in AAC31761 (PubMed:7829094).Curated | 1 | |
Sequence conflicti | 390 | T → S in AAA36425 (PubMed:1986382).Curated | 1 | |
Sequence conflicti | 471 | T → S in AAA36425 (PubMed:1986382).Curated | 1 | |
Sequence conflicti | 540 | S → T in AAA36425 (PubMed:1986382).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23499 mRNA Translation: AAC31761.1 Sequence problems. AL365361 Genomic DNA No translation available. CH471122 Genomic DNA Translation: EAW56457.1 BC035059 mRNA Translation: AAH35059.2 M55515 Genomic DNA Translation: AAA36425.1 Sequence problems. M85217 mRNA Translation: AAA59457.1 Sequence problems. M38217 Genomic DNA Translation: AAB88073.1 Different initiation. |
CCDSi | CCDS828.2 |
PIRi | A38101 |
RefSeqi | NP_002223.3, NM_002232.4 |
Genome annotation databases
Ensembli | ENST00000369769; ENSP00000358784; ENSG00000177272 |
GeneIDi | 3738 |
KEGGi | hsa:3738 |
UCSCi | uc001dzv.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23499 mRNA Translation: AAC31761.1 Sequence problems. AL365361 Genomic DNA No translation available. CH471122 Genomic DNA Translation: EAW56457.1 BC035059 mRNA Translation: AAH35059.2 M55515 Genomic DNA Translation: AAA36425.1 Sequence problems. M85217 mRNA Translation: AAA59457.1 Sequence problems. M38217 Genomic DNA Translation: AAB88073.1 Different initiation. |
CCDSi | CCDS828.2 |
PIRi | A38101 |
RefSeqi | NP_002223.3, NM_002232.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4BGC | X-ray | 1.20 | A | 104-204 | [»] | |
SMRi | P22001 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109941, 25 interactors |
DIPi | DIP-44822N |
IntActi | P22001, 5 interactors |
MINTi | P22001 |
STRINGi | 9606.ENSP00000358784 |
Chemistry databases
BindingDBi | P22001 |
ChEMBLi | CHEMBL4633 |
DrugBanki | DB00845, Clofazimine DB06637, Dalfampridine DB00228, Enflurane DB01110, Miconazole DB01069, Promethazine |
DrugCentrali | P22001 |
GuidetoPHARMACOLOGYi | 540 |
Protein family/group databases
TCDBi | 1.A.1.2.4, the voltage-gated ion channel (vic) superfamily |
PTM databases
GlyGeni | P22001, 1 site |
iPTMneti | P22001 |
PhosphoSitePlusi | P22001 |
Genetic variation databases
BioMutai | KCNA3 |
DMDMi | 215274135 |
Proteomic databases
jPOSTi | P22001 |
MassIVEi | P22001 |
MaxQBi | P22001 |
PaxDbi | P22001 |
PeptideAtlasi | P22001 |
PRIDEi | P22001 |
ProteomicsDBi | 53950 |
Protocols and materials databases
ABCDi | P22001, 69 sequenced antibodies |
Antibodypediai | 33788, 359 antibodies |
DNASUi | 3738 |
Genome annotation databases
Ensembli | ENST00000369769; ENSP00000358784; ENSG00000177272 |
GeneIDi | 3738 |
KEGGi | hsa:3738 |
UCSCi | uc001dzv.3, human |
Organism-specific databases
CTDi | 3738 |
DisGeNETi | 3738 |
GeneCardsi | KCNA3 |
HGNCi | HGNC:6221, KCNA3 |
HPAi | ENSG00000177272, Tissue enriched (lymphoid) |
MIMi | 176263, gene |
neXtProti | NX_P22001 |
OpenTargetsi | ENSG00000177272 |
PharmGKBi | PA30021 |
VEuPathDBi | HostDB:ENSG00000177272.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1545, Eukaryota |
GeneTreei | ENSGT00940000160210 |
HOGENOMi | CLU_011722_4_0_1 |
InParanoidi | P22001 |
OMAi | TTNNPNC |
OrthoDBi | 695337at2759 |
PhylomeDBi | P22001 |
TreeFami | TF313103 |
Enzyme and pathway databases
PathwayCommonsi | P22001 |
Reactomei | R-HSA-1296072, Voltage gated Potassium channels |
SIGNORi | P22001 |
Miscellaneous databases
BioGRID-ORCSi | 3738, 2 hits in 865 CRISPR screens |
GeneWikii | KCNA3 |
GenomeRNAii | 3738 |
Pharosi | P22001, Tclin |
PROi | PR:P22001 |
RNActi | P22001, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177272, Expressed in leukocyte and 107 other tissues |
Genevisiblei | P22001, HS |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003972, K_chnl_volt-dep_Kv1 IPR004050, K_chnl_volt-dep_Kv1.3 IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF00520, Ion_trans, 1 hit |
PRINTSi | PR01510, KV13CHANNEL PR01491, KVCHANNEL PR01496, SHAKERCHANEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNA3_HUMAN | |
Accessioni | P22001Primary (citable) accession number: P22001 Secondary accession number(s): Q5VWN2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | November 25, 2008 | |
Last modified: | February 10, 2021 | |
This is version 205 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families