UniProtKB - P21994 (CXB2_RAT)
Protein
Gap junction beta-2 protein
Gene
Gjb2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Structural component of gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 42 | Calcium; shared with neighboring subunitBy similarity | 1 | |
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- gap junction channel activity Source: RGD
- gap junction channel activity involved in cell communication by electrical coupling Source: RGD
- identical protein binding Source: RGD
GO - Biological processi
- aging Source: RGD
- cell-cell signaling Source: UniProtKB
- cellular response to dexamethasone stimulus Source: RGD
- cellular response to glucagon stimulus Source: RGD
- cellular response to oxidative stress Source: RGD
- decidualization Source: RGD
- epididymis development Source: RGD
- female pregnancy Source: RGD
- gap junction assembly Source: RGD
- gap junction-mediated intercellular transport Source: UniProtKB
- inner ear development Source: RGD
- response to antibiotic Source: RGD
- response to estradiol Source: RGD
- response to human chorionic gonadotropin Source: RGD
- response to ischemia Source: RGD
- response to lipopolysaccharide Source: RGD
- response to progesterone Source: RGD
- response to retinoic acid Source: RGD
- sensory perception of sound Source: UniProtKB-KW
- transmembrane transport Source: RGD
Keywordsi
Biological process | Hearing |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-190872, Transport of connexons to the plasma membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Gap junction beta-2 proteinAlternative name(s): Connexin-261 Publication Short name: Cx261 Publication |
Gene namesi | Name:Gjb2 Synonyms:Cxn-26 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 728891, Gjb2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- gap junction By similarity
Note: Colocalizes with GJB4 at gap junction plaques in the cochlea.By similarity
Cytosol
- cytosol Source: RGD
Plasma Membrane
- connexin complex Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- lateral plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- astrocyte projection Source: RGD
- cell body Source: RGD
- cell junction Source: RGD
- cytoplasm Source: RGD
- gap junction Source: RGD
- perinuclear region of cytoplasm Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Intramembranei | 2 – 13 | By similarityAdd BLAST | 12 | |
Topological domaini | 14 – 20 | CytoplasmicCurated | 7 | |
Transmembranei | 21 – 40 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 41 – 73 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 74 – 94 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 95 – 135 | CytoplasmicCuratedAdd BLAST | 41 | |
Transmembranei | 136 – 156 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 157 – 189 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 190 – 210 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 211 – 226 | CytoplasmicCuratedAdd BLAST | 16 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Gap junction, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057860 | 1 – 226 | Gap junction beta-2 proteinAdd BLAST | 226 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 53 ↔ 180 | By similarity | ||
Disulfide bondi | 60 ↔ 174 | By similarity | ||
Disulfide bondi | 64 ↔ 169 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P21994 |
PRIDEi | P21994 |
Expressioni
Tissue specificityi
Liver, kidney, intestine, lung, spleen, stomach, testis and brain, but not heart and adult skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000008855, Expressed in esophagus and 21 other tissues |
Genevisiblei | P21994, RN |
Interactioni
Subunit structurei
A hemichannel or connexon is composed of a hexamer of connexins. A functional gap junction is formed by the apposition of two hemichannels (By similarity). Forms heteromeric channels with GJB4 (By similarity).
Interacts with CNST (PubMed:19864490).
By similarity1 PublicationBinary interactionsi
P21994
With | #Exp. | IntAct |
---|---|---|
Cnst - isoform 3 [Q8CBC4-3] from Mus musculus. | 2 | EBI-2616119,EBI-2615407 |
GO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
DIPi | DIP-48901N |
IntActi | P21994, 2 interactors |
STRINGi | 10116.ENSRNOP00000011711 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QWM8, Eukaryota |
GeneTreei | ENSGT00990000203622 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | P21994 |
OMAi | YAFYFMY |
OrthoDBi | 1043502at2759 |
PhylomeDBi | P21994 |
TreeFami | TF329606 |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002268, Connexin26 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01139, CONNEXINB2 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P21994-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDWGTLQSIL GGVNKHSTSI GKIWLTVLFI FRIMILVVAA KEVWGDEQAD
60 70 80 90 100
FVCNTLQPGC KNVCYDHYFP ISHIRLWALQ LIMVSTPALL VAMHVAYRRH
110 120 130 140 150
EKKRKFMKGE IKNEFKDIEE IKTQKVRIEG SLWWTYTTSI FFRVIFEAVF
160 170 180 190 200
MYVFYIMYNG FFMQRLVKCN AWPCPNTVDC FISRPTEKTV FTVFMISVSG
210 220
ICILLNITEL CYLFIRYCSG KSKRPV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC078952 mRNA Translation: AAH78952.1 |
PIRi | A33646 |
RefSeqi | NP_001004099.1, NM_001004099.2 XP_017455254.1, XM_017599765.1 |
Genome annotation databases
Ensembli | ENSRNOT00000011711; ENSRNOP00000011711; ENSRNOG00000008855 |
GeneIDi | 394266 |
KEGGi | rno:394266 |
UCSCi | RGD:728891, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC078952 mRNA Translation: AAH78952.1 |
PIRi | A33646 |
RefSeqi | NP_001004099.1, NM_001004099.2 XP_017455254.1, XM_017599765.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
DIPi | DIP-48901N |
IntActi | P21994, 2 interactors |
STRINGi | 10116.ENSRNOP00000011711 |
Proteomic databases
PaxDbi | P21994 |
PRIDEi | P21994 |
Genome annotation databases
Ensembli | ENSRNOT00000011711; ENSRNOP00000011711; ENSRNOG00000008855 |
GeneIDi | 394266 |
KEGGi | rno:394266 |
UCSCi | RGD:728891, rat |
Organism-specific databases
CTDi | 2706 |
RGDi | 728891, Gjb2 |
Phylogenomic databases
eggNOGi | ENOG502QWM8, Eukaryota |
GeneTreei | ENSGT00990000203622 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | P21994 |
OMAi | YAFYFMY |
OrthoDBi | 1043502at2759 |
PhylomeDBi | P21994 |
TreeFami | TF329606 |
Enzyme and pathway databases
Reactomei | R-RNO-190872, Transport of connexons to the plasma membrane |
Miscellaneous databases
PROi | PR:P21994 |
Gene expression databases
Bgeei | ENSRNOG00000008855, Expressed in esophagus and 21 other tissues |
Genevisiblei | P21994, RN |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002268, Connexin26 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01139, CONNEXINB2 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CXB2_RAT | |
Accessioni | P21994Primary (citable) accession number: P21994 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families