UniProtKB - P21965 (MCK1_YEAST)
Protein
Protein kinase MCK1
Gene
MCK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.
Miscellaneous
Present with 396 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 68 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 164 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 41 – 49 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin binding Source: SGD
- enzyme inhibitor activity Source: SGD
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: SGD
- protein serine/threonine kinase activity Source: SGD
GO - Biological processi
- ascospore formation Source: SGD
- cellular protein catabolic process Source: SGD
- cellular response to DNA damage stimulus Source: SGD
- cellular response to heat Source: SGD
- cellular response to salt stress Source: SGD
- double-strand break repair via nonhomologous end joining Source: SGD
- meiotic cell cycle Source: SGD
- mitotic sister chromatid segregation Source: SGD
- peptidyl-serine phosphorylation Source: SGD
- protein phosphorylation Source: SGD
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.26, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein kinase MCK1 (EC:2.7.12.1)Alternative name(s): Meiosis and centromere regulatory kinase |
Gene namesi | Name:MCK1 Synonyms:YPK1 Ordered Locus Names:YNL307C ORF Names:N0392 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL307C |
SGDi | S000005251, MCK1 |
Subcellular locationi
Other locations
- cytoplasm Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000086317 | 2 – 375 | Protein kinase MCK1Add BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 198 | PhosphoserineCombined sources | 1 | |
Modified residuei | 199 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 202 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated at tyrosine and serine.
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P21965 |
PaxDbi | P21965 |
PRIDEi | P21965 |
PTM databases
iPTMneti | P21965 |
Interactioni
Binary interactionsi
Hide detailsGO - Molecular functioni
- cyclin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 35532, 602 interactors |
DIPi | DIP-5860N |
IntActi | P21965, 52 interactors |
MINTi | P21965 |
STRINGi | 4932.YNL307C |
Miscellaneous databases
RNActi | P21965, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 327 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 293 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0658, Eukaryota |
GeneTreei | ENSGT00520000055635 |
HOGENOMi | CLU_000288_181_20_1 |
InParanoidi | P21965 |
OMAi | LEYFFTH |
Family and domain databases
CDDi | cd14137, STKc_GSK3, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR039192, STKc_GSK3 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P21965-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV
60 70 80 90 100
QAYLTQDKKN WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT
110 120 130 140 150
HLSPQDNKVY QHLAMECLPE TLQIEINRYV TNKLEMPLKH IRLYTYQIAR
160 170 180 190 200
GMLYLHGLGV CHRDIKPSNV LVDPETGVLK ICDFGSAKKL EHNQPSISYI
210 220 230 240 250
CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF QGQEPLLQLR
260 270 280 290 300
EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG
310 320 330 340 350
IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL
360 370
FDFNDFELQI LGEFADKIKP TKVAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55054 Genomic DNA Translation: CAA38895.1 M55984 Genomic DNA Translation: AAA34764.1 Z46259 Genomic DNA Translation: CAA86388.1 Z71583 Genomic DNA Translation: CAA96236.1 Z71582 Genomic DNA Translation: CAA96235.1 BK006947 Genomic DNA Translation: DAA10254.1 |
PIRi | A39622 |
RefSeqi | NP_014092.1, NM_001183145.1 |
Genome annotation databases
EnsemblFungii | YNL307C_mRNA; YNL307C; YNL307C |
GeneIDi | 855409 |
KEGGi | sce:YNL307C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55054 Genomic DNA Translation: CAA38895.1 M55984 Genomic DNA Translation: AAA34764.1 Z46259 Genomic DNA Translation: CAA86388.1 Z71583 Genomic DNA Translation: CAA96236.1 Z71582 Genomic DNA Translation: CAA96235.1 BK006947 Genomic DNA Translation: DAA10254.1 |
PIRi | A39622 |
RefSeqi | NP_014092.1, NM_001183145.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 35532, 602 interactors |
DIPi | DIP-5860N |
IntActi | P21965, 52 interactors |
MINTi | P21965 |
STRINGi | 4932.YNL307C |
PTM databases
iPTMneti | P21965 |
Proteomic databases
MaxQBi | P21965 |
PaxDbi | P21965 |
PRIDEi | P21965 |
Genome annotation databases
EnsemblFungii | YNL307C_mRNA; YNL307C; YNL307C |
GeneIDi | 855409 |
KEGGi | sce:YNL307C |
Organism-specific databases
EuPathDBi | FungiDB:YNL307C |
SGDi | S000005251, MCK1 |
Phylogenomic databases
eggNOGi | KOG0658, Eukaryota |
GeneTreei | ENSGT00520000055635 |
HOGENOMi | CLU_000288_181_20_1 |
InParanoidi | P21965 |
OMAi | LEYFFTH |
Enzyme and pathway databases
BRENDAi | 2.7.11.26, 984 |
Miscellaneous databases
PROi | PR:P21965 |
RNActi | P21965, protein |
Family and domain databases
CDDi | cd14137, STKc_GSK3, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR039192, STKc_GSK3 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCK1_YEAST | |
Accessioni | P21965Primary (citable) accession number: P21965 Secondary accession number(s): D6W0N8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 200 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD