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Protein

Antigen peptide transporter 1

Gene

Tap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi514 – 521ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity, Peptide transport, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1236974 ER-Phagosome pathway
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 1
Short name:
APT1
Alternative name(s):
ATP-binding cassette sub-family B member 2
Histocompatibility antigen modifier 1
Peptide transporter TAP1
Gene namesi
Name:Tap1
Synonyms:Abcb2, Ham-1, Ham1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98483 Tap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini33 – 36LumenalSequence analysis4
Transmembranei37 – 59Helical; Name=2PROSITE-ProRule annotationAdd BLAST23
Topological domaini60 – 68CytoplasmicSequence analysis9
Transmembranei69 – 89Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini90 – 109LumenalSequence analysisAdd BLAST20
Transmembranei110 – 130Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini131 – 162CytoplasmicSequence analysisAdd BLAST32
Transmembranei163 – 183Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini184 – 203LumenalSequence analysisAdd BLAST20
Transmembranei204 – 224Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini225 – 274CytoplasmicSequence analysisAdd BLAST50
Transmembranei275 – 295Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini296 – 304LumenalSequence analysis9
Transmembranei305 – 325Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini326 – 394CytoplasmicSequence analysisAdd BLAST69
Transmembranei395 – 415Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini416 – 419LumenalSequence analysis4
Transmembranei420 – 440Helical; Name=10PROSITE-ProRule annotationAdd BLAST21
Topological domaini441 – 724CytoplasmicSequence analysisAdd BLAST284

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933271 – 724Antigen peptide transporter 1Add BLAST724

Proteomic databases

EPDiP21958
MaxQBiP21958
PaxDbiP21958
PeptideAtlasiP21958
PRIDEiP21958

PTM databases

iPTMnetiP21958
PhosphoSitePlusiP21958
SwissPalmiP21958

Expressioni

Gene expression databases

BgeeiENSMUSG00000037321 Expressed in 142 organ(s), highest expression level in thymus
ExpressionAtlasiP21958 baseline and differential
GenevisibleiP21958 MM

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2. Interacts with PSMB5 and PSMB8 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203966, 3 interactors
IntActiP21958, 2 interactors
MINTiP21958
STRINGi10090.ENSMUSP00000128401

Structurei

3D structure databases

ProteinModelPortaliP21958
SMRiP21958
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini163 – 446ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini479 – 718ABC transporterPROSITE-ProRule annotationAdd BLAST240

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni351 – 396Involved in peptide-binding siteBy similarityAdd BLAST46
Regioni429 – 463Involved in peptide-binding siteBy similarityAdd BLAST35

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00550000074497
HOVERGENiHBG008358
InParanoidiP21958
KOiK05653
OMAiQNQTGAI
OrthoDBiEOG091G05Q9
PhylomeDBiP21958
TreeFamiTF105197

Family and domain databases

Gene3Di1.20.1560.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR013305 ABC_Tap-like
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR013306 Tap1/ABCB2
IPR039421 Type_I_exporter
PANTHERiPTHR24221 PTHR24221, 1 hit
PTHR24221:SF249 PTHR24221:SF249, 1 hit
PfamiView protein in Pfam
PF00664 ABC_membrane, 1 hit
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF90123 SSF90123, 1 hit
TIGRFAMsiTIGR00958 3a01208, 1 hit
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P21958-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAHVWLAAA LLLLVDWLLL RPMLPGIFSL LVPEVPLLRV WVVGLSRWAI
60 70 80 90 100
LGLGVRGVLG VTAGAHGWLA ALQPLVAALS LALPGLALFR ELAAWGTLRE
110 120 130 140 150
GDSAGLLYWN SRPDAFAISY VAALPAAALW HKLGSLWAPS GNRDAGDMLC
160 170 180 190 200
RMLGFLGPKK RRLYLVLVLL ILSCLGEMAI PFFTGRITDW ILQDKTVPSF
210 220 230 240 250
TRNIWLMSIL TIASTALEFA SDGIYNITMG HMHGRVHREV FRAVLRQETG
260 270 280 290 300
FFLKNPAGSI TSRVTEDTAN VCESISGTLS LLLWYLGRAL CLLVFMFWGS
310 320 330 340 350
PYLTLVTLIN LPLLFLLPKK LGKVHQSLAV KVQESLAKST QVALEALSAM
360 370 380 390 400
PTVRSFANEE GEAQKFRQKL EEMKTLNKKE ALAYVAEVWT TSVSGMLLKV
410 420 430 440 450
GILYLGGQLV IRGAVSSGNL VSFVLYQLQF TQAVQVLLSL YPSMQKAVGS
460 470 480 490 500
SEKIFEYLDR TPCSPLSGSL APSNMKGLVE FQDVSFAYPN QPKVQVLQGL
510 520 530 540 550
TFTLHPGTVT ALVGPNGSGK STVAALLQNL YQPTGGQLLL DGQCLVQYDH
560 570 580 590 600
HYLHTQVAAV GQEPLLFGRS FRENIAYGLN RTPTMEEITA VAVESGAHDF
610 620 630 640 650
ISGFPQGYDT EVGETGNQLS GGQRQAVALA RALIRKPLLL ILDDATSALD
660 670 680 690 700
AGNQLRVQRL LYESPKRASR TVLLITQQLS LAEQAHHILF LREGSVGEQG
710 720
THLQLMKRGG CYRAMVEALA APAD
Length:724
Mass (Da):78,864
Last modified:June 28, 2011 - v3
Checksum:iA08E40F9F5FBAE9D
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TBA3Q3TBA3_MOUSE
Antigen peptide transporter 1
Tap1
696Annotation score:
F6QHF1F6QHF1_MOUSE
Antigen peptide transporter 1
Tap1
196Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277G → D in AAB41962 (PubMed:9164943).Curated1
Sequence conflicti277G → D in AAA39570 (PubMed:2270487).Curated1
Sequence conflicti277G → D in CAA42178 (Ref. 6) Curated1
Sequence conflicti355S → Q in CAA42178 (Ref. 6) Curated1
Sequence conflicti414A → T in AAB41962 (PubMed:9164943).Curated1
Sequence conflicti414A → T in AAA39570 (PubMed:2270487).Curated1
Sequence conflicti414A → T in CAA42178 (Ref. 6) Curated1
Sequence conflicti481F → L in CAA42178 (Ref. 6) Curated1
Sequence conflicti544C → R in AAB41962 (PubMed:9164943).Curated1
Sequence conflicti544C → R in AAA39570 (PubMed:2270487).Curated1
Sequence conflicti544C → R in CAA42178 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60018 mRNA Translation: AAB41962.1
U60019 mRNA Translation: AAB41963.1
AK162731 mRNA Translation: BAE37041.1
AF027865 Genomic DNA Translation: AAB81534.1
CH466660 Genomic DNA Translation: EDL10260.1
M55637 mRNA Translation: AAA39570.1
X59615 mRNA Translation: CAA42178.1
CCDSiCCDS28643.1
PIRiA37779
JC2020
RefSeqiNP_038711.2, NM_013683.2
UniGeneiMm.482076

Genome annotation databases

EnsembliENSMUST00000170086; ENSMUSP00000128401; ENSMUSG00000037321
GeneIDi21354
KEGGimmu:21354
UCSCiuc008cbu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60018 mRNA Translation: AAB41962.1
U60019 mRNA Translation: AAB41963.1
AK162731 mRNA Translation: BAE37041.1
AF027865 Genomic DNA Translation: AAB81534.1
CH466660 Genomic DNA Translation: EDL10260.1
M55637 mRNA Translation: AAA39570.1
X59615 mRNA Translation: CAA42178.1
CCDSiCCDS28643.1
PIRiA37779
JC2020
RefSeqiNP_038711.2, NM_013683.2
UniGeneiMm.482076

3D structure databases

ProteinModelPortaliP21958
SMRiP21958
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203966, 3 interactors
IntActiP21958, 2 interactors
MINTiP21958
STRINGi10090.ENSMUSP00000128401

PTM databases

iPTMnetiP21958
PhosphoSitePlusiP21958
SwissPalmiP21958

Proteomic databases

EPDiP21958
MaxQBiP21958
PaxDbiP21958
PeptideAtlasiP21958
PRIDEiP21958

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170086; ENSMUSP00000128401; ENSMUSG00000037321
GeneIDi21354
KEGGimmu:21354
UCSCiuc008cbu.2 mouse

Organism-specific databases

CTDi6890
MGIiMGI:98483 Tap1

Phylogenomic databases

eggNOGiKOG0058 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00550000074497
HOVERGENiHBG008358
InParanoidiP21958
KOiK05653
OMAiQNQTGAI
OrthoDBiEOG091G05Q9
PhylomeDBiP21958
TreeFamiTF105197

Enzyme and pathway databases

ReactomeiR-MMU-1236974 ER-Phagosome pathway
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Miscellaneous databases

PROiPR:P21958
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037321 Expressed in 142 organ(s), highest expression level in thymus
ExpressionAtlasiP21958 baseline and differential
GenevisibleiP21958 MM

Family and domain databases

Gene3Di1.20.1560.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR013305 ABC_Tap-like
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR013306 Tap1/ABCB2
IPR039421 Type_I_exporter
PANTHERiPTHR24221 PTHR24221, 1 hit
PTHR24221:SF249 PTHR24221:SF249, 1 hit
PfamiView protein in Pfam
PF00664 ABC_membrane, 1 hit
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF90123 SSF90123, 1 hit
TIGRFAMsiTIGR00958 3a01208, 1 hit
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTAP1_MOUSE
AccessioniPrimary (citable) accession number: P21958
Secondary accession number(s): Q62427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 28, 2011
Last modified: November 7, 2018
This is version 178 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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