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Protein

DNA polymerase epsilon catalytic subunit A

Gene

POL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 1970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2108ZincBy similarity1
Metal bindingi2111ZincBy similarity1
Metal bindingi2130ZincBy similarity1
Metal bindingi2133ZincBy similarity1
Metal bindingi2164Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2167Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2179Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2181Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2108 – 2133CysA-typeAdd BLAST26

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • DNA binding Source: GO_Central
  • DNA-directed DNA polymerase activity Source: SGD
  • double-stranded DNA binding Source: SGD
  • nucleotide binding Source: InterPro
  • single-stranded DNA 3'-5' exodeoxyribonuclease activity Source: SGD
  • single-stranded DNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33258-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit A
Gene namesi
Name:POL2
Synonyms:DUN2
Ordered Locus Names:YNL262W
ORF Names:N0825
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000005206 POL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi644M → I in POL2-9; temperature-sensitive mutant. 1 Publication1
Mutagenesisi710P → S in POL2-18; temperature-sensitive mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464671 – 2222DNA polymerase epsilon catalytic subunit AAdd BLAST2222

Proteomic databases

MaxQBiP21951
PaxDbiP21951
PRIDEiP21951

PTM databases

iPTMnetiP21951

Interactioni

Subunit structurei

DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DPB2P244827EBI-6140,EBI-6071

Protein-protein interaction databases

BioGridi35577, 599 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
DIPiDIP-2532N
IntActiP21951, 20 interactors
MINTiP21951
STRINGi4932.YNL262W

Structurei

Secondary structure

12222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21951
SMRiP21951
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2164 – 2181CysB motifAdd BLAST18

Domaini

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C.
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2108 – 2133CysA-typeAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000010194
HOGENOMiHOG000196287
InParanoidiP21951
KOiK02324
OMAiIIQMARQ
OrthoDBiEOG092C00WD

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR013697 DNA_pol_e_suA_C
IPR029703 POL2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10670 PTHR10670, 1 hit
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 1 hit
PF08490 DUF1744, 1 hit
SMARTiView protein in SMART
SM01159 DUF1744, 1 hit
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit

Sequencei

Sequence statusi: Complete.

P21951-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM
60 70 80 90 100
GFERYVPPQY NGRFDAKDID QIPGRVGWLT NMHATLVSQE TLSSGSNGGG
110 120 130 140 150
NSNDGERVTT NQGISGVDFY FLDEEGGSFK STVVYDPYFF IACNDESRVN
160 170 180 190 200
DVEELVKKYL ESCLKSLQII RKEDLTMDNH LLGLQKTLIK LSFVNSNQLF
210 220 230 240 250
EARKLLRPIL QDNANNNVQR NIYNVAANGS EKVDAKHLIE DIREYDVPYH
260 270 280 290 300
VRVSIDKDIR VGKWYKVTQQ GFIEDTRKIA FADPVVMAFD IETTKPPLKF
310 320 330 340 350
PDSAVDQIMM ISYMIDGEGF LITNREIISE DIEDFEYTPK PEYPGFFTIF
360 370 380 390 400
NENDEVALLQ RFFEHIRDVR PTVISTFNGD FFDWPFIHNR SKIHGLDMFD
410 420 430 440 450
EIGFAPDAEG EYKSSYCSHM DCFRWVKRDS YLPQGSQGLK AVTQSKLGYN
460 470 480 490 500
PIELDPELMT PYAFEKPQHL SEYSVSDAVA TYYLYMKYVH PFIFSLCTII
510 520 530 540 550
PLNPDETLRK GTGTLCEMLL MVQAYQHNIL LPNKHTDPIE RFYDGHLLES
560 570 580 590 600
ETYVGGHVES LEAGVFRSDL KNEFKIDPSA IDELLQELPE ALKFSVEVEN
610 620 630 640 650
KSSVDKVTNF EEIKNQITQK LLELKENNIR NELPLIYHVD VASMYPNIMT
660 670 680 690 700
TNRLQPDSIK AERDCASCDF NRPGKTCARK LKWAWRGEFF PSKMDEYNMI
710 720 730 740 750
KRALQNETFP NKNKFSKKKV LTFDELSYAD QVIHIKKRLT EYSRKVYHRV
760 770 780 790 800
KVSEIVEREA IVCQRENPFY VDTVKSFRDR RYEFKGLAKT WKGNLSKIDP
810 820 830 840 850
SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR WYSMEMAGIT
860 870 880 890 900
CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPET YFFTLENGKK
910 920 930 940 950
LYLSYPCSML NYRVHQKFTN HQYQELKDPL NYIYETHSEN TIFFEVDGPY
960 970 980 990 1000
KAMILPSSKE EGKGIKKRYA VFNEDGSLAE LKGFELKRRG ELQLIKNFQS
1010 1020 1030 1040 1050
DIFKVFLEGD TLEGCYSAVA SVCNRWLDVL DSHGLMLEDE DLVSLICENR
1060 1070 1080 1090 1100
SMSKTLKEYE GQKSTSITTA RRLGDFLGED MVKDKGLQCK YIISSKPFNA
1110 1120 1130 1140 1150
PVTERAIPVA IFSADIPIKR SFLRRWTLDP SLEDLDIRTI IDWGYYRERL
1160 1170 1180 1190 1200
GSAIQKIITI PAALQGVSNP VPRVEHPDWL KRKIATKEDK FKQTSLTKFF
1210 1220 1230 1240 1250
SKTKNVPTMG KIKDIEDLFE PTVEEDNAKI KIARTTKKKA VSKRKRNQLT
1260 1270 1280 1290 1300
NEEDPLVLPS EIPSMDEDYV GWLNYQKIKW KIQARDRKRR DQLFGNTNSS
1310 1320 1330 1340 1350
RERSALGSMI RKQAESYANS TWEVLQYKDS GEPGVLEVFV TINGKVQNIT
1360 1370 1380 1390 1400
FHIPKTIYMK FKSQTMPLQK IKNCLIEKSS ASLPNNPKTS NPAGGQLFKI
1410 1420 1430 1440 1450
TLPESVFLEE KENCTSIFND ENVLGVFEGT ITPHQRAIMD LGASVTFRSK
1460 1470 1480 1490 1500
AMGALGKGIQ QGFEMKDLSM AENERYLSGF SMDIGYLLHF PTSIGYEFFS
1510 1520 1530 1540 1550
LFKSWGDTIT ILVLKPSNQA QEINASSLGQ IYKQMFEKKK GKIETYSYLV
1560 1570 1580 1590 1600
DIKEDINFEF VYFTDISKLY RRLSQETTKL KEERGLQFLL LLQSPFITKL
1610 1620 1630 1640 1650
LGTIRLLNQM PIVKLSLNEV LLPQLNWQPT LLKKLVNHVL SSGSWISHLI
1660 1670 1680 1690 1700
KLSQYSNIPI CNLRLDSMDY IIDVLYARKL KKENIVLWWN EKAPLPDHGG
1710 1720 1730 1740 1750
IQNDFDLNTS WIMNDSEFPK INNSGVYDNV VLDVGVDNLT VNTILTSALI
1760 1770 1780 1790 1800
NDAEGSDLVN NNMGIDDKDA VINSPSEFVH DAFSNDALNV LRGMLKEWWD
1810 1820 1830 1840 1850
EALKENSTAD LLVNSLASWV QNPNAKLFDG LLRYHVHNLT KKALLQLVNE
1860 1870 1880 1890 1900
FSALGSTIVY ADRNQILIKT NKYSPENCYA YSQYMMKAVR TNPMFSYLDL
1910 1920 1930 1940 1950
NIKRYWDLLI WMDKFNFSGL ACIEIEEKEN QDYTAVSQWQ LKKFLSPIYQ
1960 1970 1980 1990 2000
PEFEDWMMII LDSMLKTKQS YLKLNSGTQR PTQIVNVKKQ DKEDSVENSL
2010 2020 2030 2040 2050
NGFSHLFSKP LMKRVKKLFK NQQEFILDPQ YEADYVIPVL PGSHLNVKNP
2060 2070 2080 2090 2100
LLELVKSLCH VMLLSKSTIL EIRTLRKELL KIFELREFAK VAEFKDPSLS
2110 2120 2130 2140 2150
LVVPDFLCEY CFFISDIDFC KAAPESIFSC VRCHKAFNQV LLQEHLIQKL
2160 2170 2180 2190 2200
RSDIESYLIQ DLRCSRCHKV KRDYMSAHCP CAGAWEGTLP RESIVQKLNV
2210 2220
FKQVAKYYGF DILLSCIADL TI
Length:2,222
Mass (Da):255,671
Last modified:August 1, 1991 - v1
Checksum:iCBCDDE2AB147D65B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60416 Genomic DNA Translation: AAA88711.1
X92494 Genomic DNA Translation: CAA63235.1
Z71538 Genomic DNA Translation: CAA96169.1
BK006947 Genomic DNA Translation: DAA10297.1
PIRiA36028
RefSeqiNP_014137.1, NM_001183100.1

Genome annotation databases

EnsemblFungiiYNL262W; YNL262W; YNL262W
GeneIDi855459
KEGGisce:YNL262W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60416 Genomic DNA Translation: AAA88711.1
X92494 Genomic DNA Translation: CAA63235.1
Z71538 Genomic DNA Translation: CAA96169.1
BK006947 Genomic DNA Translation: DAA10297.1
PIRiA36028
RefSeqiNP_014137.1, NM_001183100.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M8OX-ray2.20A1-1228[»]
4PTFX-ray2.81A1-1187[»]
5OKIX-ray4.50A/B1-524[»]
ProteinModelPortaliP21951
SMRiP21951
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35577, 599 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
DIPiDIP-2532N
IntActiP21951, 20 interactors
MINTiP21951
STRINGi4932.YNL262W

PTM databases

iPTMnetiP21951

Proteomic databases

MaxQBiP21951
PaxDbiP21951
PRIDEiP21951

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL262W; YNL262W; YNL262W
GeneIDi855459
KEGGisce:YNL262W

Organism-specific databases

SGDiS000005206 POL2

Phylogenomic databases

GeneTreeiENSGT00390000010194
HOGENOMiHOG000196287
InParanoidiP21951
KOiK02324
OMAiIIQMARQ
OrthoDBiEOG092C00WD

Enzyme and pathway databases

BioCyciYEAST:G3O-33258-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex

Miscellaneous databases

PROiPR:P21951

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR013697 DNA_pol_e_suA_C
IPR029703 POL2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10670 PTHR10670, 1 hit
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 1 hit
PF08490 DUF1744, 1 hit
SMARTiView protein in SMART
SM01159 DUF1744, 1 hit
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE_YEAST
AccessioniPrimary (citable) accession number: P21951
Secondary accession number(s): D6W0T1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 7, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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