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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene

SdhB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SdhA), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SdhB), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SdhBL), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SdhBL), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SdhAL)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi105Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi108Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi120Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi195Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi198Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi201Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi205Iron-sulfur 3 (3Fe-4S)By similarity1
Binding sitei210Ubiquinone; shared with DHSDBy similarity1
Metal bindingi252Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi258Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi262Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport, Tricarboxylic acid cycle
Ligand2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-71403 Citric acid cycle (TCA cycle)
UniPathwayi
UPA00223;UER01006

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SdhB
Synonyms:SDH
ORF Names:CG3283
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0014028 SdhB

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000010357? – 297Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiP21914
PRIDEiP21914

Expressioni

Tissue specificityi

Most abundant in the adult thorax and low in abdominal tissues.1 Publication

Developmental stagei

Expressed throughout development; pupae have very low levels of expression, in contrast to larvae.1 Publication

Gene expression databases

BgeeiFBgn0014028 Expressed in 10 organ(s), highest expression level in head
GenevisibleiP21914 DM

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Protein-protein interaction databases

BioGridi61486, 14 interactors
DIPiDIP-20115N
IntActiP21914, 3 interactors
STRINGi7227.FBpp0085489

Structurei

3D structure databases

ProteinModelPortaliP21914
SMRiP21914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 1402Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST94
Domaini185 – 2154Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST31

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3049 Eukaryota
COG0479 LUCA
GeneTreeiENSGT00390000013558
InParanoidiP21914
KOiK00235
OMAiDGQYFGP
OrthoDBiEOG091G0EKC
PhylomeDBiP21914

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di1.10.1060.10, 1 hit
3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR012675 Beta-grasp_dom_sf
IPR009051 Helical_ferredxn
IPR004489 Succ_DH/fum_Rdtase_Fe-S
IPR025192 Succ_DH/fum_Rdtase_N
PfamiView protein in Pfam
PF13085 Fer2_3, 1 hit
SUPFAMiSSF46548 SSF46548, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR00384 dhsB, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21914-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLATEARQIL SRVGSLVARN QMRAISNGTA QLEQQAQPKE AQEPQIKKFE
60 70 80 90 100
IYRWNPDNAG EKPYMQTYEV DLRECGPMVL DALIKIKNEM DPTLTFRRSC
110 120 130 140 150
REGICGSCAM NIGGTNTLAC ISKIDINTSK SLKVYPLPHM YVVRDLVPDM
160 170 180 190 200
NNFYEQYRNI QPWLQRKNEA GEKKGKAQYL QSVEDRSKLD GLYECILCAC
210 220 230 240 250
CSTSCPSYWW NAEKYLGPAV LMQAYRWIID SRDENSAERL NKLKDPFSVY
260 270 280 290
RCHTIMNCTR TCPKGLNPGR AIAEIKKLLS GLASKPAPKL ETAALHK
Length:297
Mass (Da):33,741
Last modified:February 1, 1995 - v2
Checksum:i892380E8BAE08FFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27705 Genomic DNA Translation: AAA61925.1
AE013599 Genomic DNA Translation: AAF57396.1
AY118923 mRNA Translation: AAM50783.1
RefSeqiNP_477101.1, NM_057753.5
UniGeneiDm.497

Genome annotation databases

EnsemblMetazoaiFBtr0086156; FBpp0085489; FBgn0014028
GeneIDi35590
KEGGidme:Dmel_CG3283

Similar proteinsi

Entry informationi

Entry nameiSDHB_DROME
AccessioniPrimary (citable) accession number: P21914
Secondary accession number(s): Q9V9A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: September 12, 2018
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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