Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp). Also mischarges tRNA(Asp) with D-aspartate, although it is a poor substrate (PubMed:10918062).UniRule annotation1 Publication

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171AspartateUniRule annotation1
Binding sitei217AspartateUniRule annotation1
Binding sitei226ATPUniRule annotation1
Binding sitei448AspartateUniRule annotation1
Binding sitei482ATPUniRule annotation1
Binding sitei489AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi217 – 219ATPUniRule annotation3
Nucleotide bindingi534 – 537ATPUniRule annotation4

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-UniRule
  • nucleic acid binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ASPS-MONOMER
MetaCyc:ASPS-MONOMER
BRENDAi6.1.1.12 2026
SABIO-RKiP21889

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation1 Publication)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Synonyms:tls
Ordered Locus Names:b1866, JW1855
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10097 aspS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001108681 – 590Aspartate--tRNA ligaseAdd BLAST590

Proteomic databases

EPDiP21889
PaxDbiP21889
PRIDEiP21889

2D gel databases

SWISS-2DPAGEiP21889

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259330, 32 interactors
DIPiDIP-9182N
IntActiP21889, 27 interactors
STRINGi316385.ECDH10B_2007

Chemistry databases

BindingDBiP21889

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21889
SMRiP21889
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21889

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 198AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9M Bacteria
COG0173 LUCA
HOGENOMiHOG000275159
InParanoidiP21889
KOiK01876
PhylomeDBiP21889

Family and domain databases

Gene3Di3.30.1360.30, 1 hit
HAMAPiMF_00044 Asp_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004524 Asp-tRNA-ligase_1
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR004115 GAD-like_sf
IPR029351 GAD_dom
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR22594:SF5 PTHR22594:SF5, 1 hit
PfamiView protein in Pfam
PF02938 GAD, 1 hit
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
SSF55261 SSF55261, 1 hit
TIGRFAMsiTIGR00459 aspS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P21889-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRTEYCGQLR LSHVGQQVTL CGWVNRRRDL GSLIFIDMRD REGIVQVFFD
60 70 80 90 100
PDRADALKLA SELRNEFCIQ VTGTVRARDE KNINRDMATG EIEVLASSLT
110 120 130 140 150
IINRADVLPL DSNHVNTEEA RLKYRYLDLR RPEMAQRLKT RAKITSLVRR
160 170 180 190 200
FMDDHGFLDI ETPMLTKATP EGARDYLVPS RVHKGKFYAL PQSPQLFKQL
210 220 230 240 250
LMMSGFDRYY QIVKCFRDED LRADRQPEFT QIDVETSFMT APQVREVMEA
260 270 280 290 300
LVRHLWLEVK GVDLGDFPVM TFAEAERRYG SDKPDLRNPM ELTDVADLLK
310 320 330 340 350
SVEFAVFAGP ANDPKGRVAA LRVPGGASLT RKQIDEYGNF VKIYGAKGLA
360 370 380 390 400
YIKVNERAKG LEGINSPVAK FLNAEIIEDI LDRTAAQDGD MIFFGADNKK
410 420 430 440 450
IVADAMGALR LKVGKDLGLT DESKWAPLWV IDFPMFEDDG EGGLTAMHHP
460 470 480 490 500
FTSPKDMTAA ELKAAPENAV ANAYDMVING YEVGGGSVRI HNGDMQQTVF
510 520 530 540 550
GILGINEEEQ REKFGFLLDA LKYGTPPHAG LAFGLDRLTM LLTGTDNIRD
560 570 580 590
VIAFPKTTAA ACLMTEAPSF ANPTALAELS IQVVKKAENN
Length:590
Mass (Da):65,913
Last modified:May 1, 1991 - v1
Checksum:iA411D3E16D3A9284
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53863 Genomic DNA Translation: CAA37856.1
U00096 Genomic DNA Translation: AAC74936.1
AP009048 Genomic DNA Translation: BAA15677.1
X53984 Genomic DNA Translation: CAA37932.1
D10165 Genomic DNA No translation available.
PIRiJT0942 SYECD
RefSeqiNP_416380.1, NC_000913.3
WP_001258678.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74936; AAC74936; b1866
BAA15677; BAA15677; BAA15677
GeneIDi946385
KEGGiecj:JW1855
eco:b1866
PATRICifig|1411691.4.peg.382

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53863 Genomic DNA Translation: CAA37856.1
U00096 Genomic DNA Translation: AAC74936.1
AP009048 Genomic DNA Translation: BAA15677.1
X53984 Genomic DNA Translation: CAA37932.1
D10165 Genomic DNA No translation available.
PIRiJT0942 SYECD
RefSeqiNP_416380.1, NC_000913.3
WP_001258678.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C0AX-ray2.40A1-585[»]
1EQRX-ray2.70A/B/C1-590[»]
1IL2X-ray2.60A/B1-590[»]
ProteinModelPortaliP21889
SMRiP21889
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259330, 32 interactors
DIPiDIP-9182N
IntActiP21889, 27 interactors
STRINGi316385.ECDH10B_2007

Chemistry databases

BindingDBiP21889

2D gel databases

SWISS-2DPAGEiP21889

Proteomic databases

EPDiP21889
PaxDbiP21889
PRIDEiP21889

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74936; AAC74936; b1866
BAA15677; BAA15677; BAA15677
GeneIDi946385
KEGGiecj:JW1855
eco:b1866
PATRICifig|1411691.4.peg.382

Organism-specific databases

EchoBASEiEB0095
EcoGeneiEG10097 aspS

Phylogenomic databases

eggNOGiENOG4105C9M Bacteria
COG0173 LUCA
HOGENOMiHOG000275159
InParanoidiP21889
KOiK01876
PhylomeDBiP21889

Enzyme and pathway databases

BioCyciEcoCyc:ASPS-MONOMER
MetaCyc:ASPS-MONOMER
BRENDAi6.1.1.12 2026
SABIO-RKiP21889

Miscellaneous databases

EvolutionaryTraceiP21889
PROiPR:P21889

Family and domain databases

Gene3Di3.30.1360.30, 1 hit
HAMAPiMF_00044 Asp_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004524 Asp-tRNA-ligase_1
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR004115 GAD-like_sf
IPR029351 GAD_dom
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR22594:SF5 PTHR22594:SF5, 1 hit
PfamiView protein in Pfam
PF02938 GAD, 1 hit
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
SSF55261 SSF55261, 1 hit
TIGRFAMsiTIGR00459 aspS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYD_ECOLI
AccessioniPrimary (citable) accession number: P21889
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 7, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again