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UniProtKB - P21889 (SYD_ECOLI)
Protein
Aspartate--tRNA ligase
Gene
aspS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp). Also mischarges tRNA(Asp) with D-aspartate, although it is a poor substrate (PubMed:10918062).
UniRule annotation1 PublicationCatalytic activityi
- EC:6.1.1.12UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 171 | AspartateUniRule annotation | 1 | |
Binding sitei | 217 | AspartateUniRule annotation | 1 | |
Binding sitei | 226 | ATPUniRule annotation | 1 | |
Binding sitei | 448 | AspartateUniRule annotation | 1 | |
Binding sitei | 482 | ATPUniRule annotation | 1 | |
Binding sitei | 489 | AspartateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 217 – 219 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 534 – 537 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- aspartate-tRNA ligase activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
- nucleic acid binding Source: InterPro
GO - Biological processi
- aspartyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ASPS-MONOMER |
BRENDAi | 6.1.1.12, 2026 |
SABIO-RKi | P21889 |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation1 Publication)Alternative name(s): Aspartyl-tRNA synthetaseUniRule annotation Short name: AspRSUniRule annotation |
Gene namesi | Name:aspSUniRule annotation Synonyms:tls Ordered Locus Names:b1866, JW1855 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110868 | 1 – 590 | Aspartate--tRNA ligaseAdd BLAST | 590 |
Proteomic databases
jPOSTi | P21889 |
PaxDbi | P21889 |
PRIDEi | P21889 |
2D gel databases
SWISS-2DPAGEi | P21889 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 4259330, 32 interactors |
DIPi | DIP-9182N |
IntActi | P21889, 27 interactors |
STRINGi | 511145.b1866 |
Chemistry databases
BindingDBi | P21889 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P21889 |
SMRi | P21889 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21889 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 195 – 198 | AspartateUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0173, Bacteria |
HOGENOMi | CLU_014330_3_2_6 |
InParanoidi | P21889 |
OMAi | YQLDVEM |
PhylomeDBi | P21889 |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.30.1360.30, 1 hit 3.30.930.10, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P21889-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTEYCGQLR LSHVGQQVTL CGWVNRRRDL GSLIFIDMRD REGIVQVFFD
60 70 80 90 100
PDRADALKLA SELRNEFCIQ VTGTVRARDE KNINRDMATG EIEVLASSLT
110 120 130 140 150
IINRADVLPL DSNHVNTEEA RLKYRYLDLR RPEMAQRLKT RAKITSLVRR
160 170 180 190 200
FMDDHGFLDI ETPMLTKATP EGARDYLVPS RVHKGKFYAL PQSPQLFKQL
210 220 230 240 250
LMMSGFDRYY QIVKCFRDED LRADRQPEFT QIDVETSFMT APQVREVMEA
260 270 280 290 300
LVRHLWLEVK GVDLGDFPVM TFAEAERRYG SDKPDLRNPM ELTDVADLLK
310 320 330 340 350
SVEFAVFAGP ANDPKGRVAA LRVPGGASLT RKQIDEYGNF VKIYGAKGLA
360 370 380 390 400
YIKVNERAKG LEGINSPVAK FLNAEIIEDI LDRTAAQDGD MIFFGADNKK
410 420 430 440 450
IVADAMGALR LKVGKDLGLT DESKWAPLWV IDFPMFEDDG EGGLTAMHHP
460 470 480 490 500
FTSPKDMTAA ELKAAPENAV ANAYDMVING YEVGGGSVRI HNGDMQQTVF
510 520 530 540 550
GILGINEEEQ REKFGFLLDA LKYGTPPHAG LAFGLDRLTM LLTGTDNIRD
560 570 580 590
VIAFPKTTAA ACLMTEAPSF ANPTALAELS IQVVKKAENN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53863 Genomic DNA Translation: CAA37856.1 U00096 Genomic DNA Translation: AAC74936.1 AP009048 Genomic DNA Translation: BAA15677.1 X53984 Genomic DNA Translation: CAA37932.1 D10165 Genomic DNA No translation available. |
PIRi | JT0942, SYECD |
RefSeqi | NP_416380.1, NC_000913.3 WP_001258678.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74936; AAC74936; b1866 BAA15677; BAA15677; BAA15677 |
GeneIDi | 946385 |
KEGGi | ecj:JW1855 eco:b1866 |
PATRICi | fig|1411691.4.peg.382 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53863 Genomic DNA Translation: CAA37856.1 U00096 Genomic DNA Translation: AAC74936.1 AP009048 Genomic DNA Translation: BAA15677.1 X53984 Genomic DNA Translation: CAA37932.1 D10165 Genomic DNA No translation available. |
PIRi | JT0942, SYECD |
RefSeqi | NP_416380.1, NC_000913.3 WP_001258678.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C0A | X-ray | 2.40 | A | 1-585 | [»] | |
1EQR | X-ray | 2.70 | A/B/C | 1-590 | [»] | |
1IL2 | X-ray | 2.60 | A/B | 1-590 | [»] | |
AlphaFoldDBi | P21889 | |||||
SMRi | P21889 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259330, 32 interactors |
DIPi | DIP-9182N |
IntActi | P21889, 27 interactors |
STRINGi | 511145.b1866 |
Chemistry databases
BindingDBi | P21889 |
ChEMBLi | CHEMBL4295574 |
2D gel databases
SWISS-2DPAGEi | P21889 |
Proteomic databases
jPOSTi | P21889 |
PaxDbi | P21889 |
PRIDEi | P21889 |
Genome annotation databases
EnsemblBacteriai | AAC74936; AAC74936; b1866 BAA15677; BAA15677; BAA15677 |
GeneIDi | 946385 |
KEGGi | ecj:JW1855 eco:b1866 |
PATRICi | fig|1411691.4.peg.382 |
Organism-specific databases
EchoBASEi | EB0095 |
Phylogenomic databases
eggNOGi | COG0173, Bacteria |
HOGENOMi | CLU_014330_3_2_6 |
InParanoidi | P21889 |
OMAi | YQLDVEM |
PhylomeDBi | P21889 |
Enzyme and pathway databases
BioCyci | EcoCyc:ASPS-MONOMER |
BRENDAi | 6.1.1.12, 2026 |
SABIO-RKi | P21889 |
Miscellaneous databases
EvolutionaryTracei | P21889 |
PROi | PR:P21889 |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.30.1360.30, 1 hit 3.30.930.10, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYD_ECOLI | |
Accessioni | P21889Primary (citable) accession number: P21889 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families