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UniProtKB - P21888 (SYC_ECOLI)
Protein
Cysteine--tRNA ligase
Gene
cysS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:6.1.1.16
Cofactori
Zn2+Note: Binds 1 zinc ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 28 | ZincCombined sources1 Publication | 1 | |
Metal bindingi | 209 | ZincCombined sources1 Publication | 1 | |
Metal bindingi | 234 | ZincCombined sources1 Publication | 1 | |
Metal bindingi | 238 | ZincCombined sources1 Publication | 1 | |
Binding sitei | 269 | ATPBy similarity | 1 |
GO - Molecular functioni
- aminoacyl-tRNA ligase activity Source: EcoliWiki
- ATP binding Source: EcoliWiki
- cysteine-tRNA ligase activity Source: EcoliWiki
- ligase activity Source: EcoliWiki
- metal ion binding Source: EcoliWiki
- zinc ion binding Source: EcoCyc
GO - Biological processi
- cysteinyl-tRNA aminoacylation Source: EcoliWiki
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:CYSS-MONOMER |
BRENDAi | 6.1.1.16, 2026 |
SABIO-RKi | P21888 |
Names & Taxonomyi
Protein namesi | Recommended name: Cysteine--tRNA ligase (EC:6.1.1.16)Alternative name(s): Cysteinyl-tRNA synthetase Short name: CysRS |
Gene namesi | Name:cysS Ordered Locus Names:b0526, JW0515 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159394 | 1 – 461 | Cysteine--tRNA ligaseAdd BLAST | 461 |
Proteomic databases
jPOSTi | P21888 |
PaxDbi | P21888 |
PRIDEi | P21888 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262015, 50 interactors |
IntActi | P21888, 3 interactors |
STRINGi | 511145.b0526 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P21888 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21888 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 30 – 40 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 266 – 270 | 'KMSKS' region | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | COG0215, Bacteria |
HOGENOMi | CLU_013528_0_1_6 |
InParanoidi | P21888 |
PhylomeDBi | P21888 |
Family and domain databases
CDDi | cd00672, CysRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00041, Cys_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR015803, Cys-tRNA-ligase IPR015273, Cys-tRNA-synt_Ia_DALR IPR024909, Cys-tRNA/MSH_ligase IPR014729, Rossmann-like_a/b/a_fold IPR032678, tRNA-synt_1_cat_dom IPR009080, tRNAsynth_Ia_anticodon-bd |
PANTHERi | PTHR10890, PTHR10890, 1 hit |
Pfami | View protein in Pfam PF09190, DALR_2, 1 hit PF01406, tRNA-synt_1e, 1 hit |
PRINTSi | PR00983, TRNASYNTHCYS |
SMARTi | View protein in SMART SM00840, DALR_2, 1 hit |
SUPFAMi | SSF47323, SSF47323, 1 hit |
TIGRFAMsi | TIGR00435, cysS, 1 hit |
i Sequence
Sequence statusi: Complete.
P21888-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKIFNTLTR QKEEFKPIHA GEVGMYVCGI TVYDLCHIGH GRTFVAFDVV
60 70 80 90 100
ARYLRFLGYK LKYVRNITDI DDKIIKRANE NGESFVAMVD RMIAEMHKDF
110 120 130 140 150
DALNILRPDM EPRATHHIAE IIELTEQLIA KGHAYVADNG DVMFDVPTDP
160 170 180 190 200
TYGVLSRQDL DQLQAGARVD VVDDKRNPMD FVLWKMSKEG EPSWPSPWGA
210 220 230 240 250
GRPGWHIECS AMNCKQLGNH FDIHGGGSDL MFPHHENEIA QSTCAHDGQY
260 270 280 290 300
VNYWMHSGMV MVDREKMSKS LGNFFTVRDV LKYYDAETVR YFLMSGHYRS
310 320 330 340 350
QLNYSEENLK QARAALERLY TALRGTDKTV APAGGEAFEA RFIEAMDDDF
360 370 380 390 400
NTPEAYSVLF DMAREVNRLK AEDMAAANAM ASHLRKLSAV LGLLEQEPEA
410 420 430 440 450
FLQSGAQADD SEVAEIEALI QQRLDARKAK DWAAADAARD RLNEMGIVLE
460
DGPQGTTWRR K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 316 | L → V in CAA39691 (PubMed:2014166).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56234 Genomic DNA Translation: CAA39691.1 M59381 Genomic DNA Translation: AAA23658.1 X59293 Genomic DNA Translation: CAA41983.1 U82664 Genomic DNA Translation: AAB40279.1 U00096 Genomic DNA Translation: AAC73628.1 AP009048 Genomic DNA Translation: BAE76303.1 |
PIRi | A37868, YYEC |
RefSeqi | NP_415059.1, NC_000913.3 WP_000912385.1, NZ_SSZK01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC73628; AAC73628; b0526 BAE76303; BAE76303; BAE76303 |
GeneIDi | 946969 |
KEGGi | ecj:JW0515 eco:b0526 |
PATRICi | fig|1411691.4.peg.1752 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56234 Genomic DNA Translation: CAA39691.1 M59381 Genomic DNA Translation: AAA23658.1 X59293 Genomic DNA Translation: CAA41983.1 U82664 Genomic DNA Translation: AAB40279.1 U00096 Genomic DNA Translation: AAC73628.1 AP009048 Genomic DNA Translation: BAE76303.1 |
PIRi | A37868, YYEC |
RefSeqi | NP_415059.1, NC_000913.3 WP_000912385.1, NZ_SSZK01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LI5 | X-ray | 2.30 | A/B | 1-461 | [»] | |
1LI7 | X-ray | 2.60 | A/B | 1-461 | [»] | |
1U0B | X-ray | 2.30 | B | 1-461 | [»] | |
SMRi | P21888 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262015, 50 interactors |
IntActi | P21888, 3 interactors |
STRINGi | 511145.b0526 |
Proteomic databases
jPOSTi | P21888 |
PaxDbi | P21888 |
PRIDEi | P21888 |
Genome annotation databases
EnsemblBacteriai | AAC73628; AAC73628; b0526 BAE76303; BAE76303; BAE76303 |
GeneIDi | 946969 |
KEGGi | ecj:JW0515 eco:b0526 |
PATRICi | fig|1411691.4.peg.1752 |
Organism-specific databases
EchoBASEi | EB0193 |
Phylogenomic databases
eggNOGi | COG0215, Bacteria |
HOGENOMi | CLU_013528_0_1_6 |
InParanoidi | P21888 |
PhylomeDBi | P21888 |
Enzyme and pathway databases
BioCyci | EcoCyc:CYSS-MONOMER |
BRENDAi | 6.1.1.16, 2026 |
SABIO-RKi | P21888 |
Miscellaneous databases
EvolutionaryTracei | P21888 |
PROi | PR:P21888 |
Family and domain databases
CDDi | cd00672, CysRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00041, Cys_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR015803, Cys-tRNA-ligase IPR015273, Cys-tRNA-synt_Ia_DALR IPR024909, Cys-tRNA/MSH_ligase IPR014729, Rossmann-like_a/b/a_fold IPR032678, tRNA-synt_1_cat_dom IPR009080, tRNAsynth_Ia_anticodon-bd |
PANTHERi | PTHR10890, PTHR10890, 1 hit |
Pfami | View protein in Pfam PF09190, DALR_2, 1 hit PF01406, tRNA-synt_1e, 1 hit |
PRINTSi | PR00983, TRNASYNTHCYS |
SMARTi | View protein in SMART SM00840, DALR_2, 1 hit |
SUPFAMi | SSF47323, SSF47323, 1 hit |
TIGRFAMsi | TIGR00435, cysS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYC_ECOLI | |
Accessioni | P21888Primary (citable) accession number: P21888 Secondary accession number(s): Q2MBQ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families