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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28ZincCombined sources1 Publication1
Metal bindingi209ZincCombined sources1 Publication1
Metal bindingi234ZincCombined sources1 Publication1
Metal bindingi238ZincCombined sources1 Publication1
Binding sitei269ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA ligase activity Source: EcoliWiki
  • ATP binding Source: EcoliWiki
  • cysteine-tRNA ligase activity Source: EcoliWiki
  • ligase activity Source: EcoliWiki
  • metal ion binding Source: EcoliWiki
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:CYSS-MONOMER
MetaCyc:CYSS-MONOMER
BRENDAi6.1.1.16 2026
SABIO-RKiP21888

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:b0526, JW0515
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10196 cysS

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001593941 – 461Cysteine--tRNA ligaseAdd BLAST461

Proteomic databases

EPDiP21888
PaxDbiP21888
PRIDEiP21888

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262015, 50 interactors
IntActiP21888, 3 interactors
STRINGi316385.ECDH10B_0482

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21888
SMRiP21888
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21888

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi30 – 40"HIGH" regionAdd BLAST11
Motifi266 – 270"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N Bacteria
COG0215 LUCA
HOGENOMiHOG000245250
InParanoidiP21888
KOiK01883
PhylomeDBiP21888

Family and domain databases

CDDicd00672 CysRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00041 Cys_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR015803 Cys-tRNA-ligase
IPR015273 Cys-tRNA-synt_Ia_DALR
IPR024909 Cys-tRNA/MSH_ligase
IPR014729 Rossmann-like_a/b/a_fold
IPR032678 tRNA-synt_1_cat_dom
IPR009080 tRNAsynth_Ia_anticodon-bd
PANTHERiPTHR10890 PTHR10890, 1 hit
PfamiView protein in Pfam
PF09190 DALR_2, 1 hit
PF01406 tRNA-synt_1e, 1 hit
PRINTSiPR00983 TRNASYNTHCYS
SMARTiView protein in SMART
SM00840 DALR_2, 1 hit
SUPFAMiSSF47323 SSF47323, 1 hit
TIGRFAMsiTIGR00435 cysS, 1 hit

Sequencei

Sequence statusi: Complete.

P21888-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLKIFNTLTR QKEEFKPIHA GEVGMYVCGI TVYDLCHIGH GRTFVAFDVV
60 70 80 90 100
ARYLRFLGYK LKYVRNITDI DDKIIKRANE NGESFVAMVD RMIAEMHKDF
110 120 130 140 150
DALNILRPDM EPRATHHIAE IIELTEQLIA KGHAYVADNG DVMFDVPTDP
160 170 180 190 200
TYGVLSRQDL DQLQAGARVD VVDDKRNPMD FVLWKMSKEG EPSWPSPWGA
210 220 230 240 250
GRPGWHIECS AMNCKQLGNH FDIHGGGSDL MFPHHENEIA QSTCAHDGQY
260 270 280 290 300
VNYWMHSGMV MVDREKMSKS LGNFFTVRDV LKYYDAETVR YFLMSGHYRS
310 320 330 340 350
QLNYSEENLK QARAALERLY TALRGTDKTV APAGGEAFEA RFIEAMDDDF
360 370 380 390 400
NTPEAYSVLF DMAREVNRLK AEDMAAANAM ASHLRKLSAV LGLLEQEPEA
410 420 430 440 450
FLQSGAQADD SEVAEIEALI QQRLDARKAK DWAAADAARD RLNEMGIVLE
460
DGPQGTTWRR K
Length:461
Mass (Da):52,202
Last modified:May 1, 1992 - v2
Checksum:i2FA77FDBB7C5BA99
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316L → V in CAA39691 (PubMed:2014166).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56234 Genomic DNA Translation: CAA39691.1
M59381 Genomic DNA Translation: AAA23658.1
X59293 Genomic DNA Translation: CAA41983.1
U82664 Genomic DNA Translation: AAB40279.1
U00096 Genomic DNA Translation: AAC73628.1
AP009048 Genomic DNA Translation: BAE76303.1
PIRiA37868 YYEC
RefSeqiNP_415059.1, NC_000913.3
WP_000912385.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73628; AAC73628; b0526
BAE76303; BAE76303; BAE76303
GeneIDi946969
KEGGiecj:JW0515
eco:b0526
PATRICifig|1411691.4.peg.1752

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56234 Genomic DNA Translation: CAA39691.1
M59381 Genomic DNA Translation: AAA23658.1
X59293 Genomic DNA Translation: CAA41983.1
U82664 Genomic DNA Translation: AAB40279.1
U00096 Genomic DNA Translation: AAC73628.1
AP009048 Genomic DNA Translation: BAE76303.1
PIRiA37868 YYEC
RefSeqiNP_415059.1, NC_000913.3
WP_000912385.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LI5X-ray2.30A/B1-461[»]
1LI7X-ray2.60A/B1-461[»]
1U0BX-ray2.30B1-461[»]
ProteinModelPortaliP21888
SMRiP21888
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262015, 50 interactors
IntActiP21888, 3 interactors
STRINGi316385.ECDH10B_0482

Proteomic databases

EPDiP21888
PaxDbiP21888
PRIDEiP21888

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73628; AAC73628; b0526
BAE76303; BAE76303; BAE76303
GeneIDi946969
KEGGiecj:JW0515
eco:b0526
PATRICifig|1411691.4.peg.1752

Organism-specific databases

EchoBASEiEB0193
EcoGeneiEG10196 cysS

Phylogenomic databases

eggNOGiENOG4105C8N Bacteria
COG0215 LUCA
HOGENOMiHOG000245250
InParanoidiP21888
KOiK01883
PhylomeDBiP21888

Enzyme and pathway databases

BioCyciEcoCyc:CYSS-MONOMER
MetaCyc:CYSS-MONOMER
BRENDAi6.1.1.16 2026
SABIO-RKiP21888

Miscellaneous databases

EvolutionaryTraceiP21888
PROiPR:P21888

Family and domain databases

CDDicd00672 CysRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00041 Cys_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR015803 Cys-tRNA-ligase
IPR015273 Cys-tRNA-synt_Ia_DALR
IPR024909 Cys-tRNA/MSH_ligase
IPR014729 Rossmann-like_a/b/a_fold
IPR032678 tRNA-synt_1_cat_dom
IPR009080 tRNAsynth_Ia_anticodon-bd
PANTHERiPTHR10890 PTHR10890, 1 hit
PfamiView protein in Pfam
PF09190 DALR_2, 1 hit
PF01406 tRNA-synt_1e, 1 hit
PRINTSiPR00983 TRNASYNTHCYS
SMARTiView protein in SMART
SM00840 DALR_2, 1 hit
SUPFAMiSSF47323 SSF47323, 1 hit
TIGRFAMsiTIGR00435 cysS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_ECOLI
AccessioniPrimary (citable) accession number: P21888
Secondary accession number(s): Q2MBQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1992
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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