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UniProtKB - P21874 (ODPB_GEOSE)
Protein
Pyruvate dehydrogenase E1 component subunit beta
Gene
pdhB
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activityi
- (R)-N6-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H+ + pyruvate = (R)-N6-(S8-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2EC:1.2.4.1
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 60 | Thiamine pyrophosphateBy similarity | 1 |
GO - Molecular functioni
- pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC
GO - Biological processi
- glycolytic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolysis |
Ligand | Pyruvate, Thiamine pyrophosphate |
Enzyme and pathway databases
BRENDAi | 1.2.4.1, 623 |
SABIO-RKi | P21874 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdhB |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000162221 | 2 – 325 | Pyruvate dehydrogenase E1 component subunit betaAdd BLAST | 324 |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta chain.
Binary interactionsi
P21874
With | #Exp. | IntAct |
---|---|---|
pdhA [P21873] | 3 | EBI-1040700,EBI-1040686 |
Protein-protein interaction databases
DIPi | DIP-29597N |
IntActi | P21874, 2 interactors |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P21874 |
SMRi | P21874 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21874 |
Family & Domainsi
Family and domain databases
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR033248, Transketolase_C |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit SSF52922, SSF52922, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P21874-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQMTMVQAI TDALRIELKN DPNVLIFGED VGVNGGVFRA TEGLQAEFGE
60 70 80 90 100
DRVFDTPLAE SGIGGLAIGL ALQGFRPVPE IQFFGFVYEV MDSICGQMAR
110 120 130 140 150
IRYRTGGRYH MPITIRSPFG GGVHTPELHS DSLEGLVAQQ PGLKVVIPST
160 170 180 190 200
PYDAKGLLIS AIRDNDPVIF LEHLKLYRSF RQEVPEGEYT IPIGKADIKR
210 220 230 240 250
EGKDITIIAY GAMVHESLKA AAELEKEGIS AEVVDLRTVQ PLDIETIIGS
260 270 280 290 300
VEKTGRAIVV QEAQRQAGIA ANVVAEINER AILSLEAPVL RVAAPDTVYP
310 320
FAQAESVWLP NFKDVIETAK KVMNF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53560 Genomic DNA Translation: CAA37629.1 |
PIRi | S14230 |
RefSeqi | WP_033016213.1, NZ_RCTK01000001.1 |
Genome annotation databases
GeneIDi | 58572154 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53560 Genomic DNA Translation: CAA37629.1 |
PIRi | S14230 |
RefSeqi | WP_033016213.1, NZ_RCTK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1W85 | X-ray | 2.00 | B/D/F/H | 2-325 | [»] | |
1W88 | X-ray | 2.30 | B/D/F/H | 2-325 | [»] | |
3DUF | X-ray | 2.50 | B/D/F/H | 1-325 | [»] | |
3DV0 | X-ray | 2.50 | B/D/F/H | 1-325 | [»] | |
3DVA | X-ray | 2.35 | B/D/F/H | 1-325 | [»] | |
AlphaFoldDBi | P21874 | |||||
SMRi | P21874 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29597N |
IntActi | P21874, 2 interactors |
Genome annotation databases
GeneIDi | 58572154 |
Enzyme and pathway databases
BRENDAi | 1.2.4.1, 623 |
SABIO-RKi | P21874 |
Miscellaneous databases
EvolutionaryTracei | P21874 |
Family and domain databases
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR033248, Transketolase_C |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit SSF52922, SSF52922, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ODPB_GEOSE | |
Accessioni | P21874Primary (citable) accession number: P21874 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references