UniProtKB - P21844 (CMA1_MOUSE)
Protein
Chymase
Gene
Cma1
Organism
Mus musculus (Mouse)
Status
Functioni
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Catalytic activityi
- Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa. EC:3.4.21.39
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 66 | Charge relay systemBy similarity | 1 | |
Active sitei | 110 | Charge relay systemBy similarity | 1 | |
Active sitei | 203 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- peptide binding Source: MGI
- serine-type endopeptidase activity Source: MGI
- serine-type peptidase activity Source: MGI
GO - Biological processi
- basement membrane disassembly Source: MGI
- cytokine precursor processing Source: MGI
- extracellular matrix disassembly Source: MGI
- positive regulation of angiogenesis Source: MGI
- proteolysis Source: MGI
- regulation of inflammatory response Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.B5, 3474 |
Reactomei | R-MMU-1433557, Signaling by SCF-KIT R-MMU-1592389, Activation of Matrix Metalloproteinases R-MMU-2022377, Metabolism of Angiotensinogen to Angiotensins |
Protein family/group databases
MEROPSi | S01.150 |
Names & Taxonomyi
Protein namesi | Recommended name: Chymase (EC:3.4.21.39)Alternative name(s): Alpha-chymase Mast cell chymase 1 Mast cell protease 5 Short name: mMCP-5 Mast cell protease I |
Gene namesi | Name:Cma1 Synonyms:Mcpt5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96941, Cma1 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Other locations
- Cytoplasmic granule 1 Publication
Note: Secretory granules.
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: GO_Central
Other locations
- cytoplasmic ribonucleoprotein granule Source: MGI
- secretory granule Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000027455 | 20 – 21 | Activation peptide1 Publication | 2 | |
ChainiPRO_0000027456 | 22 – 247 | ChymaseAdd BLAST | 226 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 51 ↔ 67 | PROSITE-ProRule annotation | ||
Glycosylationi | 80 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 144 ↔ 209 | PROSITE-ProRule annotation | ||
Disulfide bondi | 175 ↔ 188 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
MaxQBi | P21844 |
PaxDbi | P21844 |
PRIDEi | P21844 |
ProteomicsDBi | 285499 |
PTM databases
GlyGeni | P21844, 1 site |
iPTMneti | P21844 |
PhosphoSitePlusi | P21844 |
Expressioni
Tissue specificityi
Mast cells.
Gene expression databases
ExpressionAtlasi | P21844, baseline and differential |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000022834 |
Miscellaneous databases
RNActi | P21844, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 245 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 224 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01020000230484 |
InParanoidi | P21844 |
OMAi | PQACRHY |
OrthoDBi | 1076876at2759 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P21844-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA
60 70 80 90 100
CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP
110 120 130 140 150
KYDENLVVHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG
160 170 180 190 200
RTNVNEPASD TLQEVKMRLQ EPQACKHFTS FRHNSQLCVG NPKKMQNVYK
210 220 230 240
GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI NKILREN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA4QPC5 | A4QPC5_MOUSE | Chymase | Cma1 | 260 | Annotation score: |
Sequence cautioni
The sequence CAA48705 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | T → A in AAA40105 (PubMed:1939089).Curated | 1 | |
Sequence conflicti | 51 | C → R AA sequence (PubMed:2326280).Curated | 1 | |
Sequence conflicti | 224 | A → R in AAA39492 (PubMed:1376147).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73759 mRNA Translation: AAA40105.1 M73760 mRNA No translation available. X68805 mRNA Translation: CAA48705.1 Different initiation. M68898 mRNA Translation: AAA39492.1 AF119364 Genomic DNA Translation: AAD43901.1 |
CCDSi | CCDS27135.2 |
PIRi | S23504 S26043 |
RefSeqi | NP_034910.1, NM_010780.3 |
Genome annotation databases
Ensembli | ENSMUST00000226280; ENSMUSP00000154406; ENSMUSG00000022225 |
GeneIDi | 17228 |
KEGGi | mmu:17228 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73759 mRNA Translation: AAA40105.1 M73760 mRNA No translation available. X68805 mRNA Translation: CAA48705.1 Different initiation. M68898 mRNA Translation: AAA39492.1 AF119364 Genomic DNA Translation: AAD43901.1 |
CCDSi | CCDS27135.2 |
PIRi | S23504 S26043 |
RefSeqi | NP_034910.1, NM_010780.3 |
3D structure databases
SMRi | P21844 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000022834 |
Protein family/group databases
MEROPSi | S01.150 |
PTM databases
GlyGeni | P21844, 1 site |
iPTMneti | P21844 |
PhosphoSitePlusi | P21844 |
Proteomic databases
MaxQBi | P21844 |
PaxDbi | P21844 |
PRIDEi | P21844 |
ProteomicsDBi | 285499 |
Protocols and materials databases
Antibodypediai | 3125, 396 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000226280; ENSMUSP00000154406; ENSMUSG00000022225 |
GeneIDi | 17228 |
KEGGi | mmu:17228 |
Organism-specific databases
CTDi | 1215 |
MGIi | MGI:96941, Cma1 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01020000230484 |
InParanoidi | P21844 |
OMAi | PQACRHY |
OrthoDBi | 1076876at2759 |
Enzyme and pathway databases
BRENDAi | 3.4.21.B5, 3474 |
Reactomei | R-MMU-1433557, Signaling by SCF-KIT R-MMU-1592389, Activation of Matrix Metalloproteinases R-MMU-2022377, Metabolism of Angiotensinogen to Angiotensins |
Miscellaneous databases
BioGRID-ORCSi | 17228, 0 hits in 52 CRISPR screens |
PROi | PR:P21844 |
RNActi | P21844, protein |
SOURCEi | Search... |
Gene expression databases
ExpressionAtlasi | P21844, baseline and differential |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CMA1_MOUSE | |
Accessioni | P21844Primary (citable) accession number: P21844 Secondary accession number(s): Q9R1F0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families