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Protein

Acetylcholinesterase

Gene

Ache

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Miscellaneous

Synapses usually contain asymmetric molecules of cholinesterase, with a collagen-like part disulfide-bonded to the catalytic part. A different, globular type of cholinesterase occurs on the outer surfaces of cell membranes, including those of erythrocytes.
This is the catalytic subunit of an asymmetric or soluble form of ACHE.

Catalytic activityi

Acetylcholine + H2O = choline + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei234Acyl-ester intermediate1
Active sitei365Charge relay system1
Active sitei478Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processNeurotransmitter degradation

Enzyme and pathway databases

BRENDAi3.1.1.7 3474
ReactomeiR-MMU-112311 Neurotransmitter clearance
R-MMU-1483191 Synthesis of PC
R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin
SABIO-RKiP21836

Protein family/group databases

ESTHERimouse-ACHE AChE
MEROPSiS09.979

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
Gene namesi
Name:Ache
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:87876 Ache

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3198

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Add BLAST31
ChainiPRO_000000858832 – 614AcetylcholinesteraseAdd BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi100 ↔ 127
Disulfide bondi288 ↔ 303
Glycosylationi296N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi381N-linked (GlcNAc...) asparagine1
Disulfide bondi440 ↔ 560
Glycosylationi495N-linked (GlcNAc...) asparagine1
Disulfide bondi611InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP21836
PRIDEiP21836

PTM databases

iPTMnetiP21836
PhosphoSitePlusiP21836

Expressioni

Tissue specificityi

Predominates in most expressing tissues except erythrocytes where a glycophospholipid-attached form of ACHE predominates.

Gene expression databases

BgeeiENSMUSG00000023328 Expressed in 88 organ(s), highest expression level in medulla oblongata
CleanExiMM_ACHE
ExpressionAtlasiP21836 baseline and differential
GenevisibleiP21836 MM

Interactioni

Subunit structurei

Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers (By similarity). Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197921, 1 interactor
IntActiP21836, 2 interactors
MINTiP21836
STRINGi10090.ENSMUSP00000024099

Chemistry databases

BindingDBiP21836

Structurei

Secondary structure

1614
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21836
SMRiP21836
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21836

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4389 Eukaryota
COG2272 LUCA
GeneTreeiENSGT00760000118946
HOGENOMiHOG000091866
HOVERGENiHBG008839
InParanoidiP21836
KOiK01049
OMAiFDHYSKQ
OrthoDBiEOG091G0I4G
PhylomeDBiP21836
TreeFamiTF315470

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR014788 AChE_tetra
IPR002018 CarbesteraseB
IPR019826 Carboxylesterase_B_AS
IPR019819 Carboxylesterase_B_CS
IPR000997 Cholinesterase
PfamiView protein in Pfam
PF08674 AChE_tetra, 1 hit
PF00135 COesterase, 1 hit
PRINTSiPR00878 CHOLNESTRASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD415333 AChE_tetra, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00122 CARBOXYLESTERASE_B_1, 1 hit
PS00941 CARBOXYLESTERASE_B_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform T (identifier: P21836-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRPPWYPLHT PSLAFPLLFL LLSLLGGGAR AEGREDPQLL VRVRGGQLRG
60 70 80 90 100
IRLKAPGGPV SAFLGIPFAE PPVGSRRFMP PEPKRPWSGV LDATTFQNVC
110 120 130 140 150
YQYVDTLYPG FEGTEMWNPN RELSEDCLYL NVWTPYPRPA SPTPVLIWIY
160 170 180 190 200
GGGFYSGAAS LDVYDGRFLA QVEGAVLVSM NYRVGTFGFL ALPGSREAPG
210 220 230 240 250
NVGLLDQRLA LQWVQENIAA FGGDPMSVTL FGESAGAASV GMHILSLPSR
260 270 280 290 300
SLFHRAVLQS GTPNGPWATV SAGEARRRAT LLARLVGCPP GGAGGNDTEL
310 320 330 340 350
IACLRTRPAQ DLVDHEWHVL PQESIFRFSF VPVVDGDFLS DTPEALINTG
360 370 380 390 400
DFQDLQVLVG VVKDEGSYFL VYGVPGFSKD NESLISRAQF LAGVRIGVPQ
410 420 430 440 450
ASDLAAEAVV LHYTDWLHPE DPTHLRDAMS AVVGDHNVVC PVAQLAGRLA
460 470 480 490 500
AQGARVYAYI FEHRASTLTW PLWMGVPHGY EIEFIFGLPL DPSLNYTTEE
510 520 530 540 550
RIFAQRLMKY WTNFARTGDP NDPRDSKSPQ WPPYTTAAQQ YVSLNLKPLE
560 570 580 590 600
VRRGLRAQTC AFWNRFLPKL LSATDTLDEA ERQWKAEFHR WSSYMVHWKN
610
QFDHYSKQER CSDL
Length:614
Mass (Da):68,169
Last modified:May 1, 1991 - v1
Checksum:i66E2512463C21172
GO
Isoform H (identifier: P21836-2)
Sequence is not available
Note: No experimental confirmation available.
Length:
Mass (Da):

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JDM6A0A0G2JDM6_MOUSE
Carboxylic ester hydrolase
Ache
526Annotation score:
D3Z064D3Z064_MOUSE
Acetylcholinesterase
Ache
40Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56518 mRNA Translation: CAA39867.1
AF312033 Genomic DNA Translation: AAK28816.1
BC046327 mRNA Translation: AAH46327.1
CCDSiCCDS19763.1 [P21836-1]
PIRiJH0314
RefSeqiNP_001276939.1, NM_001290010.1 [P21836-1]
NP_033729.1, NM_009599.4 [P21836-1]
UniGeneiMm.255464
Mm.489229

Genome annotation databases

EnsembliENSMUST00000024099; ENSMUSP00000024099; ENSMUSG00000023328 [P21836-1]
ENSMUST00000085934; ENSMUSP00000083097; ENSMUSG00000023328 [P21836-1]
GeneIDi11423
KEGGimmu:11423
UCSCiuc009abt.1 mouse [P21836-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACES_MOUSE
AccessioniPrimary (citable) accession number: P21836
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 12, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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