UniProtKB - P21836 (ACES_MOUSE)
Acetylcholinesterase
Ache
Functioni
Miscellaneous
Catalytic activityi
- EC:3.1.1.7
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 234 | Acyl-ester intermediate | 1 | |
Active sitei | 365 | Charge relay system | 1 | |
Active sitei | 478 | Charge relay system | 1 |
GO - Molecular functioni
- acetylcholine binding Source: MGI
- acetylcholinesterase activity Source: MGI
- choline binding Source: MGI
- cholinesterase activity Source: MGI
- collagen binding Source: MGI
- hydrolase activity Source: MGI
- identical protein binding Source: MGI
- laminin binding Source: MGI
- protein homodimerization activity Source: MGI
- protein self-association Source: MGI
- serine hydrolase activity Source: MGI
GO - Biological processi
- acetylcholine catabolic process Source: MGI
- acetylcholine metabolic process Source: MGI
- cell adhesion Source: MGI
- choline metabolic process Source: MGI
- neuron death Source: MGI
- neurotransmitter receptor biosynthetic process Source: MGI
- osteoblast development Source: Ensembl
- positive regulation of axonogenesis Source: MGI
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of dendrite morphogenesis Source: MGI
- receptor internalization Source: MGI
- regulation of receptor recycling Source: MGI
- retina development in camera-type eye Source: MGI
- synapse assembly Source: MGI
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Neurotransmitter degradation |
Enzyme and pathway databases
BRENDAi | 3.1.1.7, 3474 |
SABIO-RKi | P21836 |
Protein family/group databases
ESTHERi | mouse-ACHE, AChE |
MEROPSi | S09.979 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ache |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87876, Ache |
Subcellular locationi
Extracellular region or secreted
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Other locations
Plasma membrane
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: MGI
- rough endoplasmic reticulum Source: MGI
Extracellular region or secreted
- basement membrane Source: MGI
- extracellular region Source: MGI
- extracellular space Source: MGI
- synaptic cleft Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nuclear envelope Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
- postsynaptic membrane Source: MGI
- presynaptic membrane Source: MGI
Other locations
- anchored component of membrane Source: UniProtKB-KW
- axon Source: MGI
- cell surface Source: MGI
- cytoplasm Source: MGI
- dendrite Source: MGI
- membrane Source: MGI
- neuromuscular junction Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: MGI
- perinuclear region of cytoplasm Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Secreted, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Add BLAST | 31 | |
ChainiPRO_0000008588 | 32 – 614 | AcetylcholinesteraseAdd BLAST | 583 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 100 ↔ 127 | |||
Disulfide bondi | 288 ↔ 303 | |||
Glycosylationi | 296 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 381 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 440 ↔ 560 | |||
Glycosylationi | 495 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 611 | InterchainBy similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | P21836 |
PRIDEi | P21836 |
PTM databases
GlyConnecti | 2100, 2 N-Linked glycans (1 site) |
GlyGeni | P21836, 3 sites |
iPTMneti | P21836 |
PhosphoSitePlusi | P21836 |
SwissPalmi | P21836 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000023328, Expressed in hypothalamus and 111 other tissues |
Genevisiblei | P21836, MM |
Interactioni
Subunit structurei
Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers (By similarity).
Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers.
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- laminin binding Source: MGI
- protein homodimerization activity Source: MGI
- protein self-association Source: MGI
Protein-protein interaction databases
BioGRIDi | 197921, 3 interactors |
IntActi | P21836, 2 interactors |
MINTi | P21836 |
STRINGi | 10090.ENSMUSP00000024099 |
Chemistry databases
BindingDBi | P21836 |
Miscellaneous databases
RNActi | P21836, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P21836 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21836 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4389, Eukaryota |
GeneTreei | ENSGT00940000157637 |
InParanoidi | P21836 |
OMAi | PWCPLHT |
OrthoDBi | 754103at2759 |
PhylomeDBi | P21836 |
TreeFami | TF315470 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR014788, AChE_tetra IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF08674, AChE_tetra, 1 hit PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPPWYPLHT PSLAFPLLFL LLSLLGGGAR AEGREDPQLL VRVRGGQLRG
60 70 80 90 100
IRLKAPGGPV SAFLGIPFAE PPVGSRRFMP PEPKRPWSGV LDATTFQNVC
110 120 130 140 150
YQYVDTLYPG FEGTEMWNPN RELSEDCLYL NVWTPYPRPA SPTPVLIWIY
160 170 180 190 200
GGGFYSGAAS LDVYDGRFLA QVEGAVLVSM NYRVGTFGFL ALPGSREAPG
210 220 230 240 250
NVGLLDQRLA LQWVQENIAA FGGDPMSVTL FGESAGAASV GMHILSLPSR
260 270 280 290 300
SLFHRAVLQS GTPNGPWATV SAGEARRRAT LLARLVGCPP GGAGGNDTEL
310 320 330 340 350
IACLRTRPAQ DLVDHEWHVL PQESIFRFSF VPVVDGDFLS DTPEALINTG
360 370 380 390 400
DFQDLQVLVG VVKDEGSYFL VYGVPGFSKD NESLISRAQF LAGVRIGVPQ
410 420 430 440 450
ASDLAAEAVV LHYTDWLHPE DPTHLRDAMS AVVGDHNVVC PVAQLAGRLA
460 470 480 490 500
AQGARVYAYI FEHRASTLTW PLWMGVPHGY EIEFIFGLPL DPSLNYTTEE
510 520 530 540 550
RIFAQRLMKY WTNFARTGDP NDPRDSKSPQ WPPYTTAAQQ YVSLNLKPLE
560 570 580 590 600
VRRGLRAQTC AFWNRFLPKL LSATDTLDEA ERQWKAEFHR WSSYMVHWKN
610
QFDHYSKQER CSDL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JDM6 | A0A0G2JDM6_MOUSE | Carboxylic ester hydrolase | Ache | 526 | Annotation score: | ||
D3Z064 | D3Z064_MOUSE | Acetylcholinesterase | Ache | 40 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56518 mRNA Translation: CAA39867.1 AF312033 Genomic DNA Translation: AAK28816.1 BC046327 mRNA Translation: AAH46327.1 |
CCDSi | CCDS19763.1 [P21836-1] |
PIRi | JH0314 |
RefSeqi | NP_001276939.1, NM_001290010.1 [P21836-1] NP_033729.1, NM_009599.4 [P21836-1] |
Genome annotation databases
Ensembli | ENSMUST00000024099; ENSMUSP00000024099; ENSMUSG00000023328 [P21836-1] ENSMUST00000085934; ENSMUSP00000083097; ENSMUSG00000023328 [P21836-1] |
GeneIDi | 11423 |
KEGGi | mmu:11423 |
UCSCi | uc009abt.1, mouse [P21836-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56518 mRNA Translation: CAA39867.1 AF312033 Genomic DNA Translation: AAK28816.1 BC046327 mRNA Translation: AAH46327.1 |
CCDSi | CCDS19763.1 [P21836-1] |
PIRi | JH0314 |
RefSeqi | NP_001276939.1, NM_001290010.1 [P21836-1] NP_033729.1, NM_009599.4 [P21836-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C2B | X-ray | 4.50 | A | 35-574 | [»] | |
1C2O | X-ray | 4.20 | A/B/C/D | 36-574 | [»] | |
1J06 | X-ray | 2.35 | A/B | 32-574 | [»] | |
1J07 | X-ray | 2.35 | A/B | 32-574 | [»] | |
1KU6 | X-ray | 2.50 | A | 32-580 | [»] | |
1MAA | X-ray | 2.90 | A/B/C/D | 32-578 | [»] | |
1MAH | X-ray | 3.20 | A | 32-574 | [»] | |
1N5M | X-ray | 2.20 | A/B | 32-572 | [»] | |
1N5R | X-ray | 2.25 | A/B | 32-574 | [»] | |
1Q83 | X-ray | 2.65 | A/B | 1-580 | [»] | |
1Q84 | X-ray | 2.45 | A/B | 1-580 | [»] | |
2C0P | X-ray | 2.50 | A/B | 32-574 | [»] | |
2C0Q | X-ray | 2.50 | A/B | 32-574 | [»] | |
2GYU | X-ray | 2.20 | A/B | 32-574 | [»] | |
2GYV | X-ray | 2.50 | A/B | 32-574 | [»] | |
2GYW | X-ray | 2.40 | A/B | 32-574 | [»] | |
2H9Y | X-ray | 2.40 | A/B | 32-574 | [»] | |
2HA0 | X-ray | 2.20 | A/B | 32-574 | [»] | |
2HA2 | X-ray | 2.05 | A/B | 32-574 | [»] | |
2HA3 | X-ray | 2.25 | A/B | 32-574 | [»] | |
2HA4 | X-ray | 2.56 | A/B | 32-574 | [»] | |
2HA5 | X-ray | 2.15 | A/B | 32-574 | [»] | |
2HA6 | X-ray | 2.25 | A/B | 32-574 | [»] | |
2HA7 | X-ray | 2.66 | A/B | 32-574 | [»] | |
2JEY | X-ray | 2.70 | A/B | 32-574 | [»] | |
2JEZ | X-ray | 2.60 | A/B | 32-574 | [»] | |
2JF0 | X-ray | 2.50 | A/B | 32-574 | [»] | |
2JGE | X-ray | 2.60 | A/B | 32-574 | [»] | |
2JGF | X-ray | 2.50 | A/B | 32-574 | [»] | |
2JGI | X-ray | 2.90 | A/B | 32-574 | [»] | |
2JGJ | X-ray | 2.50 | A/B | 32-574 | [»] | |
2JGK | X-ray | 2.90 | A/B | 32-574 | [»] | |
2JGL | X-ray | 2.60 | A/B | 32-574 | [»] | |
2JGM | X-ray | 2.90 | A/B | 32-574 | [»] | |
2WHP | X-ray | 2.20 | A/B | 32-573 | [»] | |
2WHQ | X-ray | 2.15 | A/B | 32-574 | [»] | |
2WHR | X-ray | 2.54 | A/B | 32-574 | [»] | |
2WLS | X-ray | 2.60 | A/B | 32-574 | [»] | |
2WU3 | X-ray | 2.70 | A/B | 32-574 | [»] | |
2WU4 | X-ray | 2.40 | A/B | 32-574 | [»] | |
2XUD | X-ray | 2.65 | A/B | 32-574 | [»] | |
2XUF | X-ray | 2.55 | A/B | 32-575 | [»] | |
2XUG | X-ray | 2.60 | A/B | 32-575 | [»] | |
2XUH | X-ray | 2.65 | A/B | 32-574 | [»] | |
2XUI | X-ray | 2.60 | A/B | 32-574 | [»] | |
2XUJ | X-ray | 2.65 | A/B | 32-574 | [»] | |
2XUK | X-ray | 2.75 | A/B | 32-574 | [»] | |
2XUO | X-ray | 2.80 | A/B | 32-574 | [»] | |
2XUP | X-ray | 2.70 | A/B | 32-574 | [»] | |
2XUQ | X-ray | 2.70 | A/B | 32-574 | [»] | |
2Y2U | X-ray | 2.60 | A/B | 32-574 | [»] | |
2Y2V | X-ray | 2.45 | A/B | 32-574 | [»] | |
3DL4 | X-ray | 2.50 | A/B | 32-574 | [»] | |
3DL7 | X-ray | 2.50 | A/B | 32-574 | [»] | |
3ZLT | X-ray | 2.60 | A/B | 32-574 | [»] | |
3ZLU | X-ray | 2.60 | A/B | 32-574 | [»] | |
3ZLV | X-ray | 2.50 | A/B | 32-574 | [»] | |
4A16 | X-ray | 2.65 | A/B/C/D | 35-574 | [»] | |
4A23 | X-ray | 2.40 | A/B | 32-574 | [»] | |
4ARA | X-ray | 2.50 | A/B | 32-574 | [»] | |
4ARB | X-ray | 2.25 | A/B | 32-574 | [»] | |
4B7Z | X-ray | 2.30 | A/B | 32-574 | [»] | |
4B80 | X-ray | 2.50 | A/B | 32-574 | [»] | |
4B81 | X-ray | 2.80 | A/B | 32-574 | [»] | |
4B82 | X-ray | 2.10 | A/B | 32-574 | [»] | |
4B83 | X-ray | 2.40 | A/B | 32-574 | [»] | |
4B84 | X-ray | 2.60 | A/B | 32-574 | [»] | |
4B85 | X-ray | 2.10 | A/B | 32-574 | [»] | |
4BC0 | X-ray | 3.35 | A/B/C/D | 32-574 | [»] | |
4BC1 | X-ray | 2.95 | A/B/C/D | 32-574 | [»] | |
4BTL | X-ray | 2.50 | A/B | 32-574 | [»] | |
5DTI | X-ray | 2.00 | A/B | 32-573 | [»] | |
5DTJ | X-ray | 2.71 | A/B | 32-573 | [»] | |
5EHN | X-ray | 2.60 | A/B | 32-574 | [»] | |
5EHQ | X-ray | 2.50 | A/B | 32-574 | [»] | |
5EHZ | X-ray | 2.50 | A/B | 32-574 | [»] | |
5EIA | X-ray | 2.70 | A/B | 32-574 | [»] | |
5EIE | X-ray | 2.10 | A/B | 32-574 | [»] | |
5EIH | X-ray | 2.70 | A/B | 32-574 | [»] | |
5FKJ | X-ray | 3.13 | A/B/C/D | 32-574 | [»] | |
5FPP | X-ray | 2.40 | A/B | 32-574 | [»] | |
5FUM | X-ray | 2.50 | A/B | 32-574 | [»] | |
5HCU | X-ray | 2.42 | A/B | 32-571 | [»] | |
5OV9 | X-ray | 2.40 | A/B | 32-574 | [»] | |
6FSD | X-ray | 2.70 | A/B | 32-574 | [»] | |
6FSE | X-ray | 2.70 | A/B | 32-574 | [»] | |
SMRi | P21836 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 197921, 3 interactors |
IntActi | P21836, 2 interactors |
MINTi | P21836 |
STRINGi | 10090.ENSMUSP00000024099 |
Chemistry databases
BindingDBi | P21836 |
ChEMBLi | CHEMBL3198 |
DrugCentrali | P21836 |
Protein family/group databases
ESTHERi | mouse-ACHE, AChE |
MEROPSi | S09.979 |
PTM databases
GlyConnecti | 2100, 2 N-Linked glycans (1 site) |
GlyGeni | P21836, 3 sites |
iPTMneti | P21836 |
PhosphoSitePlusi | P21836 |
SwissPalmi | P21836 |
Proteomic databases
PaxDbi | P21836 |
PRIDEi | P21836 |
Protocols and materials databases
Antibodypediai | 16701, 751 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000024099; ENSMUSP00000024099; ENSMUSG00000023328 [P21836-1] ENSMUST00000085934; ENSMUSP00000083097; ENSMUSG00000023328 [P21836-1] |
GeneIDi | 11423 |
KEGGi | mmu:11423 |
UCSCi | uc009abt.1, mouse [P21836-1] |
Organism-specific databases
CTDi | 43 |
MGIi | MGI:87876, Ache |
Phylogenomic databases
eggNOGi | KOG4389, Eukaryota |
GeneTreei | ENSGT00940000157637 |
InParanoidi | P21836 |
OMAi | PWCPLHT |
OrthoDBi | 754103at2759 |
PhylomeDBi | P21836 |
TreeFami | TF315470 |
Enzyme and pathway databases
BRENDAi | 3.1.1.7, 3474 |
SABIO-RKi | P21836 |
Miscellaneous databases
BioGRID-ORCSi | 11423, 1 hit in 17 CRISPR screens |
ChiTaRSi | Ache, mouse |
EvolutionaryTracei | P21836 |
PROi | PR:P21836 |
RNActi | P21836, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023328, Expressed in hypothalamus and 111 other tissues |
Genevisiblei | P21836, MM |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR014788, AChE_tetra IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF08674, AChE_tetra, 1 hit PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACES_MOUSE | |
Accessioni | P21836Primary (citable) accession number: P21836 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families