UniProtKB - P21734 (UBC1_YEAST)

BLAST

>sp|P21734|UBC1_YEAST Ubiquitin-conjugating enzyme E2 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UBC1 PE=1 SV=1
MSRAKRIMKEIQAVKDDPAAHITLEFVSESDIHHLKGTFLGPPGTPYEGGKFVVDIEVPM
EYPFKPPKMQFDTKVYHPNISSVTGAICLDILKNAWSPVITLKSALISLQALLQSPEPND
PQDAEVAQHYLRDRESFNKTAALWTRLYASETSNGQKGNVEESDLYGIDHDLIDEFESQG
FEKDKIVEVLRRLGVKSLDPNDNNTANRIIEELLK

Align
Format
Add to basket Added to basket
History
Entry version 181 (12 Sep 2018)
Sequence version 1 (01 May 1991)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

Ubiquitin-conjugating enzyme E2 1

Gene

UBC1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M).PROSITE-ProRule annotation2 Publications

Miscellaneous

Present with 8940 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	88	Glycyl thioester intermediate		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: GO_Central
ubiquitin protein ligase binding Source: GO_Central
ubiquitin-protein transferase activity
Source: SGD
Inferred from direct assayⁱ
- 2265617

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein polyubiquitination Source: GO_Central
ubiquitin-dependent ERAD pathway
Source: SGD
Inferred from mutant phenotypeⁱ
- 10878801
ubiquitin-dependent protein catabolic process
Source: SGD
Inferred from mutant phenotypeⁱ
- 11134048
- 2265617
vesicle organization
Source: SGD
Inferred from mutant phenotypeⁱ
- 11134048

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Stress response, Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29766-MONOMER
Reactomeⁱ	R-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 1 (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme 1 Ubiquitin carrier protein Ubiquitin-conjugating enzyme E2-24 kDa Ubiquitin-protein ligase
Gene namesⁱ	Name:UBC1 Ordered Locus Names:YDR177W ORF Names:YD9395.10
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

EuPathDBⁱ	FungiDB:YDR177W
Saccharomyces Genome Database More...SGDⁱ	S000002584 UBC1

Subcellular locationⁱ

GO - Cellular component

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000082525	1 – 215	Ubiquitin-conjugating enzyme E2 1Add BLAST		215

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	93	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P21734
PaxDbⁱ	P21734
PRIDEⁱ	P21734

PTM databases

iPTMnetⁱ	P21734

iPTMnetⁱ

P21734

Expressionⁱ

Developmental stageⁱ

Early stages of growth after spore germination.

Inductionⁱ

By heat shock and cadmium.

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
UBI4	P61864	2	EBI-19717,EBI-19797	From a different organism.

With

Entry

#Exp.

IntAct

Notes

UBI4

P61864

EBI-19717,EBI-19797

From a different organism.

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	32230, 161 interactors
Database of interacting proteins More...DIPⁱ	DIP-6594N
IntActⁱ	P21734, 6 interactors
Molecular INTeraction database More...MINTⁱ	P21734
STRINGⁱ	4932.YDR177W

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 15	Combined sources	13
Helixⁱ	18 – 20	Combined sources	3
Beta strandⁱ	22 – 27	Combined sources	6
Beta strandⁱ	28 – 31	Combined sources	4
Beta strandⁱ	32 – 40	Combined sources	9
Beta strandⁱ	43 – 45	Combined sources	3
Turnⁱ	46 – 49	Combined sources	4
Beta strandⁱ	51 – 57	Combined sources	7
Turnⁱ	60 – 63	Combined sources	4
Beta strandⁱ	68 – 73	Combined sources	6
Beta strandⁱ	78 – 80	Combined sources	3
Turnⁱ	82 – 84	Combined sources	3
Helixⁱ	90 – 92	Combined sources	3
Turnⁱ	93 – 95	Combined sources	3
Helixⁱ	102 – 114	Combined sources	13
Beta strandⁱ	118 – 120	Combined sources	3
Helixⁱ	124 – 132	Combined sources	9
Helixⁱ	134 – 148	Combined sources	15
Helixⁱ	170 – 179	Combined sources	10
Helixⁱ	183 – 192	Combined sources	10
Helixⁱ	204 – 213	Combined sources	10

Show more details

3D structure databases

ProteinModelPortalⁱ	P21734
SMRⁱ	P21734
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P21734

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00670000098059
HOGENOMⁱ	HOG000233455
InParanoidⁱ	P21734
KEGG Orthology (KO) More...KOⁱ	K04649
OMAⁱ	NKEIADC
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C50OL

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR009060 UBA-like_sf IPR015368 UBA_C_fun IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09288 UBA_3, 1 hit PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF46934 SSF46934, 1 hit SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P21734-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG 
        60         70         80         90        100
KFVVDIEVPM EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI 
       110        120        130        140        150
TLKSALISLQ ALLQSPEPND PQDAEVAQHY LRDRESFNKT AALWTRLYAS 
       160        170        180        190        200
ETSNGQKGNV EESDLYGIDH DLIDEFESQG FEKDKIVEVL RRLGVKSLDP 
       210 
NDNNTANRII EELLK

Length:215

Mass (Da):24,178

Last modified:May 1, 1991 - v1

Checksum:ⁱ899CC397A1285145

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X56402 Genomic DNA Translation: CAA39812.1 Z46727 Genomic DNA Translation: CAA86682.1 AY557675 Genomic DNA Translation: AAS56001.1 BK006938 Genomic DNA Translation: DAA12019.1
PIRⁱ	S12493
NCBI Reference Sequences More...RefSeqⁱ	NP_010462.3, NM_001180484.3

Genome annotation databases

EnsemblFungiⁱ	YDR177W; YDR177W; YDR177W
GeneIDⁱ	851757
KEGGⁱ	sce:YDR177W

Protein	Similar proteins		Species	Score	Length	Source
P21734	Ubc1p		YEASB		215	UniRef100_P21734
Ubiquitin-conjugating enzyme E2-24 kDa		YEAS1		215
K7_Ubc1p		YEASK		215
Ubc1p		YEAS8		215
Ubc1p		SACCK		215
+9

Protein	Similar proteins		Species	Score	Length	Source
P21734	Ubc1p		YEASB		215	UniRef90_P21734
Ubiquitin-conjugating enzyme E2-24 kDa		YEAS1		215
K7_Ubc1p		YEASK		215
Ubc1p		YEAS8		215
Ubc1p		SACCK		215
+15

Protein	Similar proteins		Species	Score	Length	Source
P21734	Ubc1p		YEASB		215	UniRef50_P21734
Ubiquitin-conjugating enzyme E2-24 kDa		YEAS1		215
K7_Ubc1p		YEASK		215
Ubc1p		YEAS8		215
Ubc1p		SACCK		215
+69

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X56402 Genomic DNA Translation: CAA39812.1 Z46727 Genomic DNA Translation: CAA86682.1 AY557675 Genomic DNA Translation: AAS56001.1 BK006938 Genomic DNA Translation: DAA12019.1
PIRⁱ	S12493
RefSeqⁱ	NP_010462.3, NM_001180484.3

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1FXT	NMR	-	A	2-150	[»]
	1FZY	X-ray	1.90	A/B	2-150	[»]
	1TTE	NMR	-	A	1-215	[»]
ProteinModelPortalⁱ	P21734
SMRⁱ	P21734
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32230, 161 interactors
DIPⁱ	DIP-6594N
IntActⁱ	P21734, 6 interactors
MINTⁱ	P21734
STRINGⁱ	4932.YDR177W

PTM databases

iPTMnetⁱ	P21734

iPTMnetⁱ

P21734

Proteomic databases

MaxQBⁱ	P21734
PaxDbⁱ	P21734
PRIDEⁱ	P21734

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDR177W; YDR177W; YDR177W
GeneIDⁱ	851757
KEGGⁱ	sce:YDR177W

Organism-specific databases

EuPathDBⁱ	FungiDB:YDR177W
SGDⁱ	S000002584 UBC1

Phylogenomic databases

GeneTreeⁱ	ENSGT00670000098059
HOGENOMⁱ	HOG000233455
InParanoidⁱ	P21734
KOⁱ	K04649
OMAⁱ	NKEIADC
OrthoDBⁱ	EOG092C50OL

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
BioCycⁱ	YEAST:G3O-29766-MONOMER
Reactomeⁱ	R-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

EvolutionaryTraceⁱ	P21734
Protein Ontology More...PROⁱ	PR:P21734

Family and domain databases

CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR009060 UBA-like_sf IPR015368 UBA_C_fun IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfamⁱ	View protein in Pfam PF09288 UBA_3, 1 hit PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF46934 SSF46934, 1 hit SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBC1_YEAST
Accessionⁱ	P21734Primary (citable) accession number: P21734 Secondary accession number(s): D6VSF9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
	Last sequence update:	May 1, 1991
	Last modified:	September 12, 2018
	This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P21734 (UBC1_YEAST)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Ubiquitin-conjugating enzyme E2 1

UBC1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ