UniProtKB - P21734 (UBC1_YEAST)
Protein
Ubiquitin-conjugating enzyme E2 1
Gene
UBC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M).PROSITE-ProRule annotation2 Publications
Miscellaneous
Present with 8940 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 88 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- proteasome binding Source: SGD
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
- ubiquitin-dependent ERAD pathway Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
- vesicle organization Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Stress response, Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 1 (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme 1 Ubiquitin carrier protein Ubiquitin-conjugating enzyme E2-24 kDa Ubiquitin-protein ligase |
Gene namesi | Name:UBC1 Ordered Locus Names:YDR177W ORF Names:YD9395.10 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002584, UBC1 |
VEuPathDBi | FungiDB:YDR177W |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082525 | 1 – 215 | Ubiquitin-conjugating enzyme E2 1Add BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 93 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P21734 |
PaxDbi | P21734 |
PRIDEi | P21734 |
PTM databases
iPTMneti | P21734 |
Expressioni
Developmental stagei
Early stages of growth after spore germination.
Inductioni
By heat shock and cadmium.
Interactioni
Protein-protein interaction databases
BioGRIDi | 32230, 163 interactors |
DIPi | DIP-6594N |
IntActi | P21734, 6 interactors |
MINTi | P21734 |
STRINGi | 4932.YDR177W |
Miscellaneous databases
RNActi | P21734, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P21734 |
SMRi | P21734 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21734 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 150 | UBC corePROSITE-ProRule annotationAdd BLAST | 149 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0418, Eukaryota |
GeneTreei | ENSGT00940000170562 |
HOGENOMi | CLU_030988_13_1_1 |
InParanoidi | P21734 |
OMAi | DTARYWT |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
DisProti | DP02193 |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR009060, UBA-like_sf IPR015368, UBA_C_fun IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF09288, UBA_3, 1 hit PF00179, UQ_con, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P21734-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG
60 70 80 90 100
KFVVDIEVPM EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI
110 120 130 140 150
TLKSALISLQ ALLQSPEPND PQDAEVAQHY LRDRESFNKT AALWTRLYAS
160 170 180 190 200
ETSNGQKGNV EESDLYGIDH DLIDEFESQG FEKDKIVEVL RRLGVKSLDP
210
NDNNTANRII EELLK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56402 Genomic DNA Translation: CAA39812.1 Z46727 Genomic DNA Translation: CAA86682.1 AY557675 Genomic DNA Translation: AAS56001.1 BK006938 Genomic DNA Translation: DAA12019.1 |
PIRi | S12493 |
RefSeqi | NP_010462.3, NM_001180484.3 |
Genome annotation databases
EnsemblFungii | YDR177W_mRNA; YDR177W; YDR177W |
GeneIDi | 851757 |
KEGGi | sce:YDR177W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56402 Genomic DNA Translation: CAA39812.1 Z46727 Genomic DNA Translation: CAA86682.1 AY557675 Genomic DNA Translation: AAS56001.1 BK006938 Genomic DNA Translation: DAA12019.1 |
PIRi | S12493 |
RefSeqi | NP_010462.3, NM_001180484.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FXT | NMR | - | A | 2-150 | [»] | |
1FZY | X-ray | 1.90 | A/B | 2-150 | [»] | |
1TTE | NMR | - | A | 1-215 | [»] | |
BMRBi | P21734 | |||||
SMRi | P21734 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32230, 163 interactors |
DIPi | DIP-6594N |
IntActi | P21734, 6 interactors |
MINTi | P21734 |
STRINGi | 4932.YDR177W |
PTM databases
iPTMneti | P21734 |
Proteomic databases
MaxQBi | P21734 |
PaxDbi | P21734 |
PRIDEi | P21734 |
Genome annotation databases
EnsemblFungii | YDR177W_mRNA; YDR177W; YDR177W |
GeneIDi | 851757 |
KEGGi | sce:YDR177W |
Organism-specific databases
SGDi | S000002584, UBC1 |
VEuPathDBi | FungiDB:YDR177W |
Phylogenomic databases
eggNOGi | KOG0418, Eukaryota |
GeneTreei | ENSGT00940000170562 |
HOGENOMi | CLU_030988_13_1_1 |
InParanoidi | P21734 |
OMAi | DTARYWT |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
EvolutionaryTracei | P21734 |
PROi | PR:P21734 |
RNActi | P21734, protein |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
DisProti | DP02193 |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR009060, UBA-like_sf IPR015368, UBA_C_fun IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF09288, UBA_3, 1 hit PF00179, UQ_con, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBC1_YEAST | |
Accessioni | P21734Primary (citable) accession number: P21734 Secondary accession number(s): D6VSF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families