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Protein

Gastrin-releasing peptide receptor

Gene

Grpr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for gastrin-releasing peptide (GRP) (PubMed:1671171, PubMed:1707129, PubMed:9345264, PubMed:12526815, PubMed:26658875). Signals via association with G proteins that activate a phosphatidylinositol-calcium second messenger system, resulting in Akt phosphorylation (PubMed:26658875). Contributes to the regulation of food intake (PubMed:12176666). Contributes to the perception of prurient stimuli and transmission of itch signals in the spinal cord that promote scratching behavior, but does not play a role in the perception of pain (PubMed:28280205, PubMed:17653196, PubMed:26658875). Contributes primarily to nonhistaminergic itch sensation (PubMed:28280205). Contributes to long-term fear memory, but not normal spatial memory (PubMed:12526815).7 Publications

GO - Molecular functioni

  • bombesin receptor activity Source: MGI
  • G-protein coupled peptide receptor activity Source: UniProtKB
  • G-protein coupled receptor activity Source: MGI
  • neuropeptide binding Source: MGI
  • neuropeptide receptor activity Source: UniProtKB

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: MGI
  • learning or memory Source: MGI
  • motor behavior Source: UniProtKB
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  • psychomotor behavior Source: UniProtKB
  • regulation of cell proliferation Source: MGI
  • response to external biotic stimulus Source: UniProtKB
  • social behavior Source: UniProtKB

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-416476 G alpha (q) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Gastrin-releasing peptide receptor
Short name:
GRP-R
Alternative name(s):
GRP-preferring bombesin receptor
Gene namesi
Name:Grpr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95836 Grpr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 39ExtracellularSequence analysisAdd BLAST39
Transmembranei40 – 63Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini64 – 77CytoplasmicSequence analysisAdd BLAST14
Transmembranei78 – 97Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini98 – 115ExtracellularSequence analysisAdd BLAST18
Transmembranei116 – 137Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini138 – 153CytoplasmicSequence analysisAdd BLAST16
Transmembranei154 – 175Helical; Name=4Sequence analysisAdd BLAST22
Topological domaini176 – 209ExtracellularSequence analysisAdd BLAST34
Transmembranei210 – 235Helical; Name=5Sequence analysisAdd BLAST26
Topological domaini236 – 265CytoplasmicSequence analysisAdd BLAST30
Transmembranei266 – 286Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini287 – 299ExtracellularSequence analysisAdd BLAST13
Transmembranei300 – 326Helical; Name=7Sequence analysisAdd BLAST27
Topological domaini327 – 384CytoplasmicSequence analysisAdd BLAST58

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate, are viable and fertile (PubMed:9345264). They do not decrease their food intake in response to gastrin-releasing peptide (PubMed:12176666). Mice consume more food per meal, but the overall food intake per day remains unchanged (PubMed:12176666). Still, they display increased body weight relative to wild-type (PubMed:12176666). Contrary to wild-type mice, their body temparature remains nearly constant when exposed to cold (4 degrees Celsius) after intracerebroventricular injection of gastrin-releasing peptide (PubMed:9345264). Mutant mice show increased locomotor activity and increased social interactions (PubMed:9345264). They do not show any change in the perception of painful stimuli, but show reduced scratching in response to pruritogenic treatments (PubMed:17653196). Contrary to wild-type, mutant mice do not display imitative scratching after observing spontaneous scratching behavior in another mouse (PubMed:28280205). Mutant mice display normal performance in a water maze, but show decreased inhibition of principal neurons by the interneurons, enhanced long-term potentiation (LTP), and greater and more persistent long-term fear memory (PubMed:12526815).5 Publications

Chemistry databases

ChEMBLiCHEMBL3596
GuidetoPHARMACOLOGYi39

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000696631 – 384Gastrin-releasing peptide receptorAdd BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi20N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi114 ↔ 197PROSITE-ProRule annotation
Lipidationi340S-palmitoyl cysteineBy similarity1
Modified residuei351PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP21729
PRIDEiP21729

PTM databases

PhosphoSitePlusiP21729
SwissPalmiP21729

Expressioni

Tissue specificityi

Detected in pancreas and brain (PubMed:1671171, PubMed:9345264). Detected in suprachaismatic nucleus neurons (PubMed:28280205). Detected in neurons in the dorsal horn of the spinal cord (PubMed:17653196). Detected in inhibitory GABAergic interneurons in the lateral nuclues of the amydala (PubMed:12526815).5 Publications

Gene expression databases

BgeeiENSMUSG00000031364
CleanExiMM_GRPR
GenevisibleiP21729 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Oprm1P42866-94EBI-6049651,EBI-6049667

Protein-protein interaction databases

IntActiP21729, 1 interactor
STRINGi10090.ENSMUSP00000033730

Chemistry databases

BindingDBiP21729

Structurei

3D structure databases

ProteinModelPortaliP21729
SMRiP21729
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000119177
HOGENOMiHOG000230781
HOVERGENiHBG004898
InParanoidiP21729
KOiK04169
OMAiYYFIARN
OrthoDBiEOG091G085Y
PhylomeDBiP21729
TreeFamiTF331292

Family and domain databases

CDDicd15124 7tmA_GRPR, 1 hit
InterProiView protein in InterPro
IPR001556 Bombsn_rcpt-like
IPR001966 Gastrin_pep_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00358 BOMBESINR
PR00640 GASTRINRELPR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P21729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPNNCSHLN LDVDPFLSCN DTFNQSLSPP KMDNWFHPGF IYVIPAVYGL
60 70 80 90 100
IIVIGLIGNI TLIKIFCTVK SMRNVPNLFI SSLALGDLLL LVTCAPVDAS
110 120 130 140 150
KYLADRWLFG RIGCKLIPFI QLTSVGVSVF TLTALSADRY KAIVRPMDIQ
160 170 180 190 200
ASHALMKICL KAALIWIVSM LLAIPEAVFS DLHPFHVKDT NQTFISCAPY
210 220 230 240 250
PHSNELHPKI HSMASFLVFY VIPLAIISVY YYFIARNLIQ SAYNLPVEGN
260 270 280 290 300
IHVKKQIESR KRLAKTVLVF VGLFAFCWLP NHVIYLYRSY HYSEVDTSML
310 320 330 340 350
HFVTSICARL LAFTNSCVNP FALYLLSKSF RKQFNTQLLC CQPGLMNRSH
360 370 380
STGRSTTCMT SFKSTNPSAT FSLINRNICH EGYV
Length:384
Mass (Da):43,215
Last modified:August 1, 1991 - v2
Checksum:iBF6D60387AA09A2C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309R → H (PubMed:1707129).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57922 mRNA Translation: AAA75650.1
M61000 mRNA Translation: AAA37744.1
AH007139, U84263 Genomic DNA Translation: AAD00557.1
AK033473 mRNA Translation: BAC28307.1
CH466571 Genomic DNA Translation: EDL40772.1
BC113145 mRNA Translation: AAI13146.1
CCDSiCCDS30512.1
PIRiA39003
RefSeqiNP_032203.1, NM_008177.3
UniGeneiMm.4687

Genome annotation databases

EnsembliENSMUST00000033730; ENSMUSP00000033730; ENSMUSG00000031364
GeneIDi14829
KEGGimmu:14829
UCSCiuc009uuu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGRPR_MOUSE
AccessioniPrimary (citable) accession number: P21729
Secondary accession number(s): Q53WV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 1, 1991
Last modified: June 20, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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