UniProtKB - P21693 (DBPA_ECOLI)
Protein
ATP-dependent RNA helicase DbpA
Gene
dbpA
Organism
Escherichia coli (strain K12)
Status
Functioni
DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes. Requires a single-stranded RNA loading site on the 3' side of the substrate helix.UniRule annotation9 Publications
Catalytic activityi
- EC:3.6.4.13UniRule annotation4 Publications
Activity regulationi
Requires hairpin 92 of 23S rRNA for optimal activity. ATPase activity is stimulated by interaction of the N-terminal domain with RNA.3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 47 – 54 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- 3'-5' RNA helicase activity Source: EcoCyc
- ADP binding Source: EcoCyc
- ATPase activity Source: EcoliWiki
- ATP binding Source: EcoCyc
- DNA/RNA helicase activity Source: EcoliWiki
- RNA helicase activity Source: EcoliWiki
- rRNA binding Source: EcoCyc
GO - Biological processi
- ribosomal large subunit assembly Source: EcoCyc
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10210-MONOMER MetaCyc:EG10210-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dbpAUniRule annotation Ordered Locus Names:b1343, JW1337 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 53 | K → A: Shows accumulation of partially-assembled 45S particles. 1 Publication | 1 | |
Mutagenesisi | 154 | E → A: Shows accumulation of partially-assembled 45S particles. 1 Publication | 1 | |
Mutagenesisi | 331 | R → A: Shows accumulation of partially-assembled 45S particles. Binds rRNA normally but is severely impaired in ATPase and helicase activities. Overexpression confers a dominant slow growth and cold-sensitive phenotype. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055096 | 1 – 457 | ATP-dependent RNA helicase DbpAAdd BLAST | 457 |
Proteomic databases
jPOSTi | P21693 |
PaxDbi | P21693 |
PRIDEi | P21693 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262985, 99 interactors |
IntActi | P21693, 6 interactors |
STRINGi | 511145.b1343 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 205 | Helicase ATP-bindingUniRule annotationAdd BLAST | 172 | |
Domaini | 230 – 376 | Helicase C-terminalUniRule annotationAdd BLAST | 147 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 383 – 457 | Involved in 23S rRNA bindingUniRule annotationAdd BLAST | 75 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 3 – 31 | Q motifAdd BLAST | 29 | |
Motifi | 153 – 156 | DEAD box | 4 |
Domaini
Contains an N-terminal domain that binds non-specifically to RNA and a C-terminal domain that binds specifically and tightly to hairpin 92 of 23S rRNA.UniRule annotation2 Publications
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0513, Bacteria |
HOGENOMi | CLU_003041_1_3_6 |
InParanoidi | P21693 |
PhylomeDBi | P21693 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
HAMAPi | MF_00965, DEAD_helicase_DbpA, 1 hit |
InterProi | View protein in InterPro IPR005580, DbpA/CsdA_RNA-bd_dom IPR011545, DEAD/DEAH_box_helicase_dom IPR028619, DEAD_helicase_DbpA IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR012677, Nucleotide-bd_a/b_plait_sf IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF03880, DbpA, 1 hit PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
P21693-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTAFSTLNVL PPAQLTNLNE LGYLTMTPVQ AAALPAILAG KDVRVQAKTG
60 70 80 90 100
SGKTAAFGLG LLQQIDASLF QTQALVLCPT RELADQVAGE LRRLARFLPN
110 120 130 140 150
TKILTLCGGQ PFGMQRDSLQ HAPHIIVATP GRLLDHLQKG TVSLDALNTL
160 170 180 190 200
VMDEADRMLD MGFSDAIDDV IRFAPASRQT LLFSATWPEA IAAISGRVQR
210 220 230 240 250
DPLAIEIDST DALPPIEQQF YETSSKGKIP LLQRLLSLHQ PSSCVVFCNT
260 270 280 290 300
KKDCQAVCDA LNEVGQSALS LHGDLEQRDR DQTLVRFANG SARVLVATDV
310 320 330 340 350
AARGLDIKSL ELVVNFELAW DPEVHVHRIG RTARAGNSGL AISFCAPEEA
360 370 380 390 400
QRANIISDML QIKLNWQTPP ANSSIATLEA EMATLCIDGG KKAKMRPGDV
410 420 430 440 450
LGALTGDIGL DGADIGKIAV HPAHVYVAVR QAVAHKAWKQ LQGGKIKGKT
CRVRLLK
Sequence cautioni
The sequence CAA36872 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52647 Genomic DNA Translation: CAA36872.1 Different initiation. U00096 Genomic DNA Translation: AAC74425.1 AP009048 Genomic DNA Translation: BAA14946.1 |
PIRi | B64884 |
RefSeqi | NP_415859.1, NC_000913.3 WP_000123737.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74425; AAC74425; b1343 BAA14946; BAA14946; BAA14946 |
GeneIDi | 57732113 947153 |
KEGGi | ecj:JW1337 eco:b1343 |
PATRICi | fig|1411691.4.peg.933 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52647 Genomic DNA Translation: CAA36872.1 Different initiation. U00096 Genomic DNA Translation: AAC74425.1 AP009048 Genomic DNA Translation: BAA14946.1 |
PIRi | B64884 |
RefSeqi | NP_415859.1, NC_000913.3 WP_000123737.1, NZ_SSZK01000012.1 |
3D structure databases
SMRi | P21693 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262985, 99 interactors |
IntActi | P21693, 6 interactors |
STRINGi | 511145.b1343 |
Proteomic databases
jPOSTi | P21693 |
PaxDbi | P21693 |
PRIDEi | P21693 |
Genome annotation databases
EnsemblBacteriai | AAC74425; AAC74425; b1343 BAA14946; BAA14946; BAA14946 |
GeneIDi | 57732113 947153 |
KEGGi | ecj:JW1337 eco:b1343 |
PATRICi | fig|1411691.4.peg.933 |
Organism-specific databases
EchoBASEi | EB0206 |
Phylogenomic databases
eggNOGi | COG0513, Bacteria |
HOGENOMi | CLU_003041_1_3_6 |
InParanoidi | P21693 |
PhylomeDBi | P21693 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10210-MONOMER MetaCyc:EG10210-MONOMER |
Miscellaneous databases
PROi | PR:P21693 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
HAMAPi | MF_00965, DEAD_helicase_DbpA, 1 hit |
InterProi | View protein in InterPro IPR005580, DbpA/CsdA_RNA-bd_dom IPR011545, DEAD/DEAH_box_helicase_dom IPR028619, DEAD_helicase_DbpA IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR012677, Nucleotide-bd_a/b_plait_sf IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF03880, DbpA, 1 hit PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DBPA_ECOLI | |
Accessioni | P21693Primary (citable) accession number: P21693 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families