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UniProtKB - P21673 (SAT1_HUMAN)

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Protein

Diamine acetyltransferase 1

Gene

SAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N1-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N1- and N8-acetylspermidine), spermine, N1-acetylspermidine and N8-acetylspermidine.2 Publications

Catalytic activityi

Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.2 Publications

Kineticsi

  1. KM=3.8 µM for acetyl-coenzyme A1 Publication
  2. KM=5.7 µM for spermine1 Publication
  3. KM=22 µM for spermidine1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Pathwayi: putrescine degradation

    This protein is involved in step 1 of the subpathway that synthesizes N-acetylputrescine from putrescine.
    Proteins known to be involved in this subpathway in this organism are:
    1. Diamine acetyltransferase 2 (SAT2), Diamine acetyltransferase 1 (SAT1)
    This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetylputrescine from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei92Substrate; via carbonyl oxygen1 Publication1
    Binding sitei152Substrate1 Publication1

    GO - Molecular functioni

    View the complete GO annotation on QuickGO ...

    GO - Biological processi

    View the complete GO annotation on QuickGO ...

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:HS05339-MONOMER
    BRENDAi2.3.1.57 2681
    ReactomeiR-HSA-351200 Interconversion of polyamines
    SIGNORiP21673
    UniPathwayiUPA00188; UER00363

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diamine acetyltransferase 1 (EC:2.3.1.572 Publications)
    Alternative name(s):
    Polyamine N-acetyltransferase 1
    Putrescine acetyltransferase
    Spermidine/spermine N(1)-acetyltransferase 1
    Short name:
    SSAT
    Short name:
    SSAT-1
    Gene namesi
    Name:SAT1
    Synonyms:SAT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
    Proteomesi
    • UP000005640 Componenti: Chromosome X

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000130066.16
    HGNCiHGNC:10540 SAT1
    MIMi313020 gene
    neXtProtiNX_P21673

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Involvement in diseasei

    Keratosis follicularis spinulosa decalvans X-linked (KFSDX)2 Publications
    The disease may be caused by mutations affecting the gene represented in this entry.
    Disease descriptionA rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration.
    See also OMIM:308800

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi140Y → F: Reduces activity by 95%. 1 Publication1

    Organism-specific databases

    DisGeNETi6303
    MalaCardsiSAT1
    MIMi308800 phenotype
    OpenTargetsiENSG00000130066
    Orphaneti2340 Keratosis follicularis spinulosa decalvans
    PharmGKBiPA162402389

    Chemistry databases

    ChEMBLiCHEMBL4286
    DrugBankiDB00127 Spermine

    Polymorphism and mutation databases

    BioMutaiSAT1
    DMDMi114322

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000745911 – 171Diamine acetyltransferase 1Add BLAST171

    Proteomic databases

    PaxDbiP21673
    PeptideAtlasiP21673
    PRIDEiP21673
    ProteomicsDBi53885

    PTM databases

    iPTMnetiP21673
    PhosphoSitePlusiP21673

    Expressioni

    Gene expression databases

    BgeeiENSG00000130066
    CleanExiHS_SAT1
    ExpressionAtlasiP21673 baseline and differential
    GenevisibleiP21673 HS

    Organism-specific databases

    HPAiCAB047343
    CAB069914
    HPA055312

    Interactioni

    Subunit structurei

    Homodimer.3 Publications

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • identical protein binding Source: IntAct
    View the complete GO annotation on QuickGO ...

    Protein-protein interaction databases

    BioGridi112210, 73 interactors
    DIPiDIP-36801N
    IntActiP21673, 83 interactors
    MINTiP21673
    STRINGi9606.ENSP00000368572

    Chemistry databases

    BindingDBiP21673

    Structurei

    Secondary structure

    1171
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 8Combined sources4
    Helixi11 – 13Combined sources3
    Helixi14 – 25Combined sources12
    Beta strandi28 – 30Combined sources3
    Helixi31 – 33Combined sources3
    Helixi38 – 45Combined sources8
    Beta strandi46 – 49Combined sources4
    Beta strandi53 – 58Combined sources6
    Helixi61 – 63Combined sources3
    Beta strandi71 – 82Combined sources12
    Turni83 – 85Combined sources3
    Beta strandi86 – 96Combined sources11
    Helixi98 – 100Combined sources3
    Beta strandi102 – 104Combined sources3
    Helixi105 – 120Combined sources16
    Beta strandi123 – 130Combined sources8
    Helixi134 – 141Combined sources8
    Turni142 – 144Combined sources3
    Helixi148 – 152Combined sources5
    Beta strandi154 – 160Combined sources7
    Helixi161 – 168Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    		PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2B3UX-ray1.85A/B1-171[»]
    2B3VX-ray1.95A1-171[»]
    2B4BX-ray2.00A/B1-171[»]
    2B4DX-ray2.00A/B1-171[»]
    2B58X-ray1.95A1-171[»]
    2B5GX-ray1.70A/B1-171[»]
    2F5IX-ray2.30A/B1-171[»]
    2FXFX-ray2.00A/B2-171[»]
    2G3TX-ray1.80A/B1-171[»]
    2JEVX-ray2.30A/B1-171[»]
    ProteinModelPortaliP21673
    SMRiP21673
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21673

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini4 – 171N-acetyltransferasePROSITE-ProRule annotationAdd BLAST168

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni27 – 28Substrate binding1 Publication2
    Regioni94 – 96Acetyl-CoA binding1 Publication3
    Regioni102 – 107Acetyl-CoA binding1 Publication6
    Regioni126 – 128Substrate binding1 Publication3
    Regioni133 – 136Acetyl-CoA binding1 Publication4
    Regioni140 – 143Acetyl-CoA binding1 Publication4

    Sequence similaritiesi

    Belongs to the acetyltransferase family.Curated

    Phylogenomic databases

    eggNOGiKOG3216 Eukaryota
    COG0454 LUCA
    GeneTreeiENSGT00440000039972
    HOGENOMiHOG000078521
    HOVERGENiHBG063175
    InParanoidiP21673
    KOiK00657
    OMAiVKCRCSS
    OrthoDBiEOG091G0UNU
    PhylomeDBiP21673
    TreeFamiTF319736

    Family and domain databases

    InterProiView protein in InterPro
    IPR016181 Acyl_CoA_acyltransferase
    IPR000182 GNAT_dom
    IPR032957 SAT1
    PANTHERiPTHR10545:SF36 PTHR10545:SF36, 1 hit
    PfamiView protein in Pfam
    PF00583 Acetyltransf_1, 1 hit
    SUPFAMiSSF55729 SSF55729, 1 hit
    PROSITEiView protein in PROSITE
    PS51186 GNAT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P21673-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP 
            60         70         80         90        100
    FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY 
           110        120        130        140        150
    RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS 
           160        170 
    EEGWRLFKID KEYLLKMATE E
    Length:171
    Mass (Da):20,024
    Last modified:May 1, 1991 - v1
    Checksum:i6A0578B88CD72F09
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti26K → E in CAA78509 (PubMed:1417826).Curated1
    Sequence conflicti26K → E in AAA98854 (PubMed:8573111).Curated1
    Sequence conflicti69H → G AA sequence (PubMed:2241897).Curated1
    Sequence conflicti84W → P AA sequence (PubMed:2241897).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		M77693 mRNA Translation: AAA60573.1
    M55580 mRNA Translation: AAA63260.1
    Z14136 Genomic DNA Translation: CAA78509.1
    U40369 Genomic DNA Translation: AAA98854.1
    BT006825 mRNA Translation: AAP35471.1
    CR450294 mRNA Translation: CAG29290.1
    AK312162 mRNA Translation: BAG35096.1
    CH471074 Genomic DNA Translation: EAW99000.1
    BC002503 mRNA Translation: AAH02503.1
    BC008424 mRNA Translation: AAH08424.1
    CCDSiCCDS14207.1
    PIRiJH0783
    RefSeqiNP_002961.1, NM_002970.3
    UniGeneiHs.28491

    Genome annotation databases

    EnsembliENST00000379270; ENSP00000368572; ENSG00000130066
    GeneIDi6303
    KEGGihsa:6303
    UCSCiuc004dau.5 human

    Similar proteinsi

    Entry informationi

    Entry nameiSAT1_HUMAN
    AccessioniPrimary (citable) accession number: P21673
    Secondary accession number(s): Q6ICU9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: June 20, 2018
    This is version 180 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

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