UniProtKB - P21673 (SAT1_HUMAN)
Diamine acetyltransferase 1
SAT1
Functioni
Enzyme which catalyzes the acetylation of polyamines (PubMed:15283699, PubMed:16455797, PubMed:17516632).
Substrate specificity: norspermidine = spermidine >> spermine > N1-acetylspermine > putrescine (PubMed:17516632).
This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines (PubMed:16455797).
Also involved in the regulation of polyamine transport out of cells (PubMed:16455797).
Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N1- and N8-acetylspermidine), spermine, N1-acetylspermidine and N8-acetylspermidine (PubMed:16455797, PubMed:17516632).
3 PublicationsCatalytic activityi
- EC:2.3.1.573 PublicationsThis reaction proceeds in the forward3 Publications direction.
- EC:2.3.1.572 PublicationsThis reaction proceeds in the forward2 Publications direction.
- EC:2.3.1.572 PublicationsThis reaction proceeds in the forward2 Publications direction.
Kineticsi
- KM=3.8 µM for acetyl-coenzyme A1 Publication
- KM=5.7 µM for spermine1 Publication
- KM=3.7 µM for spermine1 Publication
- KM=22 µM for spermidine1 Publication
- KM=58 µM for spermidine1 Publication
pH dependencei
: putrescine degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N-acetylputrescine from putrescine.2 Publications This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetylputrescine from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | Substrate; via carbonyl oxygen1 Publication | 1 | |
Active sitei | 140 | Proton donorBy similarity | 1 | |
Binding sitei | 152 | Substrate1 Publication | 1 |
GO - Molecular functioni
- diamine N-acetyltransferase activity Source: UniProtKB
- identical protein binding Source: IntAct
- N-acetyltransferase activity Source: UniProtKB
- spermidine binding Source: GO_Central
GO - Biological processi
- angiogenesis Source: UniProtKB
- polyamine biosynthetic process Source: UniProtKB
- putrescine catabolic process Source: UniProtKB-UniPathway
- spermidine acetylation Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.57, 2681 |
PathwayCommonsi | P21673 |
Reactomei | R-HSA-351200, Interconversion of polyamines |
SignaLinki | P21673 |
SIGNORi | P21673 |
UniPathwayi | UPA00188;UER00363 |
Names & Taxonomyi
Protein namesi | Recommended name: Diamine acetyltransferase 1Curated (EC:2.3.1.573 Publications)Alternative name(s): Polyamine N-acetyltransferase 1Curated Putrescine acetyltransferase1 Publication Spermidine/spermine N(1)-acetyltransferase 12 Publications Short name: SSAT2 Publications Short name: SSAT-1 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10540, SAT1 |
MIMi | 313020, gene |
neXtProti | NX_P21673 |
VEuPathDBi | HostDB:ENSG00000130066 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 2 Publications
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Involvement in diseasei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 140 | Y → F: Reduces activity by 95%. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6303 |
MalaCardsi | SAT1 |
OpenTargetsi | ENSG00000130066 |
Orphaneti | 2340, Keratosis follicularis spinulosa decalvans |
PharmGKBi | PA162402389 |
Miscellaneous databases
Pharosi | P21673, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4286 |
DrugBanki | DB04633, N-ethyl-N-[3-(propylamino)propyl]propane-1,3-diamine DB00127, Spermine |
DrugCentrali | P21673 |
Genetic variation databases
BioMutai | SAT1 |
DMDMi | 114322 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074591 | 1 – 171 | Diamine acetyltransferase 1Add BLAST | 171 |
Proteomic databases
EPDi | P21673 |
MassIVEi | P21673 |
PaxDbi | P21673 |
PeptideAtlasi | P21673 |
PRIDEi | P21673 |
ProteomicsDBi | 53885 |
PTM databases
iPTMneti | P21673 |
PhosphoSitePlusi | P21673 |
Expressioni
Gene expression databases
Bgeei | ENSG00000130066, Expressed in palpebral conjunctiva and 240 other tissues |
ExpressionAtlasi | P21673, baseline and differential |
Genevisiblei | P21673, HS |
Organism-specific databases
HPAi | ENSG00000130066, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsBinary interactionsi
P21673
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 112210, 103 interactors |
DIPi | DIP-36801N |
IntActi | P21673, 93 interactors |
MINTi | P21673 |
STRINGi | 9606.ENSP00000368572 |
Chemistry databases
BindingDBi | P21673 |
Miscellaneous databases
RNActi | P21673, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P21673 |
SMRi | P21673 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21673 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 171 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 168 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 27 – 28 | Substrate binding1 Publication | 2 | |
Regioni | 94 – 96 | Acetyl-CoA binding1 Publication | 3 | |
Regioni | 102 – 107 | Acetyl-CoA binding1 Publication | 6 | |
Regioni | 126 – 128 | Substrate binding1 Publication | 3 | |
Regioni | 133 – 136 | Acetyl-CoA binding1 Publication | 4 | |
Regioni | 140 – 143 | Acetyl-CoA binding1 Publication | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3216, Eukaryota |
GeneTreei | ENSGT00950000183121 |
HOGENOMi | CLU_013985_41_1_1 |
InParanoidi | P21673 |
OMAi | AVERQCG |
OrthoDBi | 1228251at2759 |
PhylomeDBi | P21673 |
TreeFami | TF319736 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR032957, SAT1 |
PANTHERi | PTHR10545:SF36, PTHR10545:SF36, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP
60 70 80 90 100
FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY
110 120 130 140 150
RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS
160 170
EEGWRLFKID KEYLLKMATE E
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PD37 | E9PD37_HUMAN | Diamine acetyltransferase 1 | SAT1 | 143 | Annotation score: | ||
Q2TM25 | Q2TM25_HUMAN | Diamine acetyltransferase 1 | SAT1 SAT | 71 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | K → E in CAA78509 (PubMed:1417826).Curated | 1 | |
Sequence conflicti | 26 | K → E in AAA98854 (PubMed:8573111).Curated | 1 | |
Sequence conflicti | 69 | H → G AA sequence (PubMed:2241897).Curated | 1 | |
Sequence conflicti | 84 | W → P AA sequence (PubMed:2241897).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M77693 mRNA Translation: AAA60573.1 M55580 mRNA Translation: AAA63260.1 Z14136 Genomic DNA Translation: CAA78509.1 U40369 Genomic DNA Translation: AAA98854.1 BT006825 mRNA Translation: AAP35471.1 CR450294 mRNA Translation: CAG29290.1 AK312162 mRNA Translation: BAG35096.1 CH471074 Genomic DNA Translation: EAW99000.1 BC002503 mRNA Translation: AAH02503.1 BC008424 mRNA Translation: AAH08424.1 |
CCDSi | CCDS14207.1 |
PIRi | JH0783 |
RefSeqi | NP_002961.1, NM_002970.3 |
Genome annotation databases
Ensembli | ENST00000379270.5; ENSP00000368572.4; ENSG00000130066.17 |
GeneIDi | 6303 |
KEGGi | hsa:6303 |
MANE-Selecti | ENST00000379270.5; ENSP00000368572.4; NM_002970.4; NP_002961.1 |
UCSCi | uc004dau.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M77693 mRNA Translation: AAA60573.1 M55580 mRNA Translation: AAA63260.1 Z14136 Genomic DNA Translation: CAA78509.1 U40369 Genomic DNA Translation: AAA98854.1 BT006825 mRNA Translation: AAP35471.1 CR450294 mRNA Translation: CAG29290.1 AK312162 mRNA Translation: BAG35096.1 CH471074 Genomic DNA Translation: EAW99000.1 BC002503 mRNA Translation: AAH02503.1 BC008424 mRNA Translation: AAH08424.1 |
CCDSi | CCDS14207.1 |
PIRi | JH0783 |
RefSeqi | NP_002961.1, NM_002970.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2B3U | X-ray | 1.85 | A/B | 1-171 | [»] | |
2B3V | X-ray | 1.95 | A | 1-171 | [»] | |
2B4B | X-ray | 2.00 | A/B | 1-171 | [»] | |
2B4D | X-ray | 2.00 | A/B | 1-171 | [»] | |
2B58 | X-ray | 1.95 | A | 1-171 | [»] | |
2B5G | X-ray | 1.70 | A/B | 1-171 | [»] | |
2F5I | X-ray | 2.30 | A/B | 1-171 | [»] | |
2FXF | X-ray | 2.00 | A/B | 2-171 | [»] | |
2G3T | X-ray | 1.80 | A/B | 1-171 | [»] | |
2JEV | X-ray | 2.30 | A/B | 1-171 | [»] | |
AlphaFoldDBi | P21673 | |||||
SMRi | P21673 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112210, 103 interactors |
DIPi | DIP-36801N |
IntActi | P21673, 93 interactors |
MINTi | P21673 |
STRINGi | 9606.ENSP00000368572 |
Chemistry databases
BindingDBi | P21673 |
ChEMBLi | CHEMBL4286 |
DrugBanki | DB04633, N-ethyl-N-[3-(propylamino)propyl]propane-1,3-diamine DB00127, Spermine |
DrugCentrali | P21673 |
PTM databases
iPTMneti | P21673 |
PhosphoSitePlusi | P21673 |
Genetic variation databases
BioMutai | SAT1 |
DMDMi | 114322 |
Proteomic databases
EPDi | P21673 |
MassIVEi | P21673 |
PaxDbi | P21673 |
PeptideAtlasi | P21673 |
PRIDEi | P21673 |
ProteomicsDBi | 53885 |
Protocols and materials databases
Antibodypediai | 24525, 258 antibodies from 22 providers |
DNASUi | 6303 |
Genome annotation databases
Ensembli | ENST00000379270.5; ENSP00000368572.4; ENSG00000130066.17 |
GeneIDi | 6303 |
KEGGi | hsa:6303 |
MANE-Selecti | ENST00000379270.5; ENSP00000368572.4; NM_002970.4; NP_002961.1 |
UCSCi | uc004dau.5, human |
Organism-specific databases
CTDi | 6303 |
DisGeNETi | 6303 |
GeneCardsi | SAT1 |
HGNCi | HGNC:10540, SAT1 |
HPAi | ENSG00000130066, Low tissue specificity |
MalaCardsi | SAT1 |
MIMi | 313020, gene |
neXtProti | NX_P21673 |
OpenTargetsi | ENSG00000130066 |
Orphaneti | 2340, Keratosis follicularis spinulosa decalvans |
PharmGKBi | PA162402389 |
VEuPathDBi | HostDB:ENSG00000130066 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3216, Eukaryota |
GeneTreei | ENSGT00950000183121 |
HOGENOMi | CLU_013985_41_1_1 |
InParanoidi | P21673 |
OMAi | AVERQCG |
OrthoDBi | 1228251at2759 |
PhylomeDBi | P21673 |
TreeFami | TF319736 |
Enzyme and pathway databases
UniPathwayi | UPA00188;UER00363 |
BRENDAi | 2.3.1.57, 2681 |
PathwayCommonsi | P21673 |
Reactomei | R-HSA-351200, Interconversion of polyamines |
SignaLinki | P21673 |
SIGNORi | P21673 |
Miscellaneous databases
BioGRID-ORCSi | 6303, 15 hits in 712 CRISPR screens |
ChiTaRSi | SAT1, human |
EvolutionaryTracei | P21673 |
GeneWikii | SAT1_(gene) |
GenomeRNAii | 6303 |
Pharosi | P21673, Tbio |
PROi | PR:P21673 |
RNActi | P21673, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000130066, Expressed in palpebral conjunctiva and 240 other tissues |
ExpressionAtlasi | P21673, baseline and differential |
Genevisiblei | P21673, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR032957, SAT1 |
PANTHERi | PTHR10545:SF36, PTHR10545:SF36, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SAT1_HUMAN | |
Accessioni | P21673Primary (citable) accession number: P21673 Secondary accession number(s): Q6ICU9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 205 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families