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Protein

Pyruvate kinase II

Gene

pykA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK.

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase A (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. Probable phosphoglycerate mutase GpmB (gpmB), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase I (pykF), Pyruvate kinase II (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Metal bindingi38PotassiumBy similarity1
Metal bindingi40PotassiumBy similarity1
Metal bindingi70PotassiumBy similarity1
Sitei223Transition state stabilizerBy similarity1
Metal bindingi225MagnesiumBy similarity1
Binding sitei251Substrate; via amide nitrogenBy similarity1
Metal bindingi252MagnesiumBy similarity1
Binding sitei252Substrate; via amide nitrogenBy similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • potassium ion binding Source: InterPro
  • pyruvate kinase activity Source: EcoCyc

GO - Biological processi

  • protein homotetramerization Source: EcoCyc

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciEcoCyc:PKII-MONOMER
MetaCyc:PKII-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase II (EC:2.7.1.40)
Alternative name(s):
PK-2
Gene namesi
Name:pykA
Ordered Locus Names:b1854, JW1843
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10803 pykA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001120732 – 480Pyruvate kinase IIAdd BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei351N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP21599
PaxDbiP21599
PRIDEiP21599

PTM databases

iPTMnetiP21599

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4263205, 27 interactors
DIPiDIP-10622N
IntActiP21599, 18 interactors
STRINGi316385.ECDH10B_1995

Structurei

3D structure databases

ProteinModelPortaliP21599
SMRiP21599
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
HOGENOMiHOG000021558
InParanoidiP21599
KOiK00873
OMAiICVEAEK
PhylomeDBiP21599

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLRRTKI VTTLGPATDR DNNLEKVIAA GANVVRMNFS HGSPEDHKMR
60 70 80 90 100
ADKVREIAAK LGRHVAILGD LQGPKIRVST FKEGKVFLNI GDKFLLDANL
110 120 130 140 150
GKGEGDKEKV GIDYKGLPAD VVPGDILLLD DGRVQLKVLE VQGMKVFTEV
160 170 180 190 200
TVGGPLSNNK GINKLGGGLS AEALTEKDKA DIKTAALIGV DYLAVSFPRC
210 220 230 240 250
GEDLNYARRL ARDAGCDAKI VAKVERAEAV CSQDAMDDII LASDVVMVAR
260 270 280 290 300
GDLGVEIGDP ELVGIQKALI RRARQLNRAV ITATQMMESM ITNPMPTRAE
310 320 330 340 350
VMDVANAVLD GTDAVMLSAE TAAGQYPSET VAAMARVCLG AEKIPSINVS
360 370 380 390 400
KHRLDVQFDN VEEAIAMSAM YAANHLKGVT AIITMTESGR TALMTSRISS
410 420 430 440 450
GLPIFAMSRH ERTLNLTALY RGVTPVHFDS ANDGVAAASE AVNLLRDKGY
460 470 480
LMSGDLVIVT QGDVMSTVGS TNTTRILTVE
Length:480
Mass (Da):51,357
Last modified:January 23, 2007 - v3
Checksum:iC37F004C374D27E9
GO

Sequence cautioni

The sequence AAA96707 differs from that shown. Reason: Frameshift at position 441.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20R → C AA sequence (PubMed:1859631).Curated1
Sequence conflicti43S → G AA sequence (PubMed:1859631).Curated1
Sequence conflicti323A → R in AAA96707 (Ref. 6) Curated1
Sequence conflicti383Missing in AAA96707 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63703 Genomic DNA Translation: AAA24473.1
U00096 Genomic DNA Translation: AAC74924.1
AP009048 Genomic DNA Translation: BAA15662.1
M77039 Genomic DNA No translation available.
M87660 Genomic DNA Translation: AAA96707.1 Frameshift.
PIRiS29790
RefSeqiNP_416368.1, NC_000913.3
WP_000091148.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74924; AAC74924; b1854
BAA15662; BAA15662; BAA15662
GeneIDi946527
KEGGiecj:JW1843
eco:b1854
PATRICifig|511145.12.peg.1933

Similar proteinsi

Entry informationi

Entry nameiKPYK2_ECOLI
AccessioniPrimary (citable) accession number: P21599
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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