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Protein

5'-nucleotidase

Gene

NT5E

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Zinc 11
Metal bindingi38Zinc 11
Metal bindingi85Zinc 11
Metal bindingi85Zinc 21
Metal bindingi117Zinc 21
Sitei118Transition state stabilizer1
Sitei121Transition state stabilizerCurated1
Metal bindingi220Zinc 21
Metal bindingi243Zinc 21
Binding sitei245Substrate1
Binding sitei354Substrate1
Binding sitei390Substrate1
Binding sitei395Substrate1
Binding sitei417Substrate1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS05981-MONOMER
ReactomeiR-HSA-196807 Nicotinate metabolism
R-HSA-73621 Pyrimidine catabolism
R-HSA-74259 Purine catabolism
SIGNORiP21589

Names & Taxonomyi

Protein namesi
Recommended name:
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Alternative name(s):
Ecto-5'-nucleotidase
CD_antigen: CD73
Gene namesi
Name:NT5E
Synonyms:NT5, NTE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000135318.11
HGNCiHGNC:8021 NT5E
MIMi129190 gene
neXtProtiNX_P21589

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Calcification of joints and arteries (CALJA)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
See also OMIM:211800
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_065185358C → Y in CALJA; absence from the plasma-membrane; exhibits no catalytic AMPase activity. 2 PublicationsCorresponds to variant dbSNP:rs387906620EnsemblClinVar.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi4907
MalaCardsiNT5E
MIMi211800 phenotype
OpenTargetsiENSG00000135318
Orphaneti289601 Hereditary arterial and articular multiple calcification syndrome
PharmGKBiPA31804

Chemistry databases

ChEMBLiCHEMBL5957
DrugBankiDB00987 Cytarabine
DB00806 Pentoxifylline
GuidetoPHARMACOLOGYi1232

Polymorphism and mutation databases

BioMutaiNT5E
DMDMi112825

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000000001527 – 5495'-nucleotidase1 PublicationAdd BLAST523
PropeptideiPRO_0000000016550 – 574Removed in mature formAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 571 Publication
Glycosylationi53N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi311N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi333N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi353 ↔ 3581 Publication
Disulfide bondi365 ↔ 3871 Publication
Glycosylationi403N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi476 ↔ 4791 Publication
Lipidationi549GPI-anchor amidated serine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP21589
MaxQBiP21589
PaxDbiP21589
PeptideAtlasiP21589
PRIDEiP21589
ProteomicsDBi53883
53884 [P21589-2]

PTM databases

DEPODiP21589
iPTMnetiP21589
PhosphoSitePlusiP21589
SwissPalmiP21589

Expressioni

Gene expression databases

BgeeiENSG00000135318 Expressed in 220 organ(s), highest expression level in endometrium
CleanExiHS_NT5E
ExpressionAtlasiP21589 baseline and differential
GenevisibleiP21589 HS

Organism-specific databases

HPAiHPA017357

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi110962, 49 interactors
DIPiDIP-59992N
IntActiP21589, 4 interactors
STRINGi9606.ENSP00000257770

Chemistry databases

BindingDBiP21589

Structurei

Secondary structure

1574
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21589
SMRiP21589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni500 – 506Substrate binding7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4419 Eukaryota
COG0737 LUCA
GeneTreeiENSGT00530000063775
HOGENOMiHOG000247215
HOVERGENiHBG000026
KOiK19970
OMAiKRLFKPY
OrthoDBiEOG091G08IK
PhylomeDBiP21589
TreeFamiTF323589

Family and domain databases

Gene3Di3.60.21.10, 1 hit
3.90.780.10, 1 hit
InterProiView protein in InterPro
IPR008334 5'-Nucleotdase_C
IPR036907 5'-Nucleotdase_C_sf
IPR006146 5'-Nucleotdase_CS
IPR006179 5_nucleotidase/apyrase
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
PANTHERiPTHR11575 PTHR11575, 1 hit
PfamiView protein in Pfam
PF02872 5_nucleotid_C, 1 hit
PF00149 Metallophos, 1 hit
PRINTSiPR01607 APYRASEFAMLY
SUPFAMiSSF55816 SSF55816, 1 hit
PROSITEiView protein in PROSITE
PS00785 5_NUCLEOTIDASE_1, 1 hit
PS00786 5_NUCLEOTIDASE_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P21589-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCPRAARAPA TLLLALGAVL WPAAGAWELT ILHTNDVHSR LEQTSEDSSK
60 70 80 90 100
CVNASRCMGG VARLFTKVQQ IRRAEPNVLL LDAGDQYQGT IWFTVYKGAE
110 120 130 140 150
VAHFMNALRY DAMALGNHEF DNGVEGLIEP LLKEAKFPIL SANIKAKGPL
160 170 180 190 200
ASQISGLYLP YKVLPVGDEV VGIVGYTSKE TPFLSNPGTN LVFEDEITAL
210 220 230 240 250
QPEVDKLKTL NVNKIIALGH SGFEMDKLIA QKVRGVDVVV GGHSNTFLYT
260 270 280 290 300
GNPPSKEVPA GKYPFIVTSD DGRKVPVVQA YAFGKYLGYL KIEFDERGNV
310 320 330 340 350
ISSHGNPILL NSSIPEDPSI KADINKWRIK LDNYSTQELG KTIVYLDGSS
360 370 380 390 400
QSCRFRECNM GNLICDAMIN NNLRHTDEMF WNHVSMCILN GGGIRSPIDE
410 420 430 440 450
RNNGTITWEN LAAVLPFGGT FDLVQLKGST LKKAFEHSVH RYGQSTGEFL
460 470 480 490 500
QVGGIHVVYD LSRKPGDRVV KLDVLCTKCR VPSYDPLKMD EVYKVILPNF
510 520 530 540 550
LANGGDGFQM IKDELLRHDS GDQDINVVST YISKMKVIYP AVEGRIKFST
560 570
GSHCHGSFSL IFLSLWAVIF VLYQ
Length:574
Mass (Da):63,368
Last modified:May 1, 1991 - v1
Checksum:iA99AF170AB7EAECE
GO
Isoform 2 (identifier: P21589-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-453: Missing.

Note: No experimental confirmation available.
Show »
Length:524
Mass (Da):57,949
Checksum:i98A11D22CFC7C610
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q96B60Q96B60_HUMAN
5'-nucleotidase
NT5E hCG_401111
264Annotation score:
H0Y3X5H0Y3X5_HUMAN
5'-nucleotidase
NT5E
179Annotation score:
H0Y7R7H0Y7R7_HUMAN
5'-nucleotidase
NT5E
230Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_065185358C → Y in CALJA; absence from the plasma-membrane; exhibits no catalytic AMPase activity. 2 PublicationsCorresponds to variant dbSNP:rs387906620EnsemblClinVar.1
Natural variantiVAR_022091376T → A1 PublicationCorresponds to variant dbSNP:rs2229523Ensembl.1
Natural variantiVAR_048103379M → T. Corresponds to variant dbSNP:rs2229524Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043076404 – 453Missing in isoform 2. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55740 mRNA Translation: CAA39271.1
AK075008 mRNA Translation: BAG52050.1
AL135903 Genomic DNA No translation available.
AL589666 Genomic DNA No translation available.
U21730 Genomic DNA Translation: AAA96950.1
AF069067 Genomic DNA Translation: AAC98672.1
CCDSiCCDS5002.1 [P21589-1]
CCDS56439.1 [P21589-2]
PIRiS11032
RefSeqiNP_001191742.1, NM_001204813.1 [P21589-2]
NP_002517.1, NM_002526.3 [P21589-1]
UniGeneiHs.153952

Genome annotation databases

EnsembliENST00000257770; ENSP00000257770; ENSG00000135318 [P21589-1]
ENST00000369651; ENSP00000358665; ENSG00000135318 [P21589-2]
GeneIDi4907
KEGGihsa:4907
UCSCiuc003pko.5 human [P21589-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55740 mRNA Translation: CAA39271.1
AK075008 mRNA Translation: BAG52050.1
AL135903 Genomic DNA No translation available.
AL589666 Genomic DNA No translation available.
U21730 Genomic DNA Translation: AAA96950.1
AF069067 Genomic DNA Translation: AAC98672.1
CCDSiCCDS5002.1 [P21589-1]
CCDS56439.1 [P21589-2]
PIRiS11032
RefSeqiNP_001191742.1, NM_001204813.1 [P21589-2]
NP_002517.1, NM_002526.3 [P21589-1]
UniGeneiHs.153952

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H1SX-ray2.20A/B27-549[»]
4H1YX-ray1.58P27-549[»]
4H2BX-ray1.70A27-549[»]
4H2FX-ray1.85A27-549[»]
4H2GX-ray1.55A27-549[»]
4H2IX-ray2.00A27-549[»]
ProteinModelPortaliP21589
SMRiP21589
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110962, 49 interactors
DIPiDIP-59992N
IntActiP21589, 4 interactors
STRINGi9606.ENSP00000257770

Chemistry databases

BindingDBiP21589
ChEMBLiCHEMBL5957
DrugBankiDB00987 Cytarabine
DB00806 Pentoxifylline
GuidetoPHARMACOLOGYi1232

PTM databases

DEPODiP21589
iPTMnetiP21589
PhosphoSitePlusiP21589
SwissPalmiP21589

Polymorphism and mutation databases

BioMutaiNT5E
DMDMi112825

Proteomic databases

EPDiP21589
MaxQBiP21589
PaxDbiP21589
PeptideAtlasiP21589
PRIDEiP21589
ProteomicsDBi53883
53884 [P21589-2]

Protocols and materials databases

DNASUi4907
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257770; ENSP00000257770; ENSG00000135318 [P21589-1]
ENST00000369651; ENSP00000358665; ENSG00000135318 [P21589-2]
GeneIDi4907
KEGGihsa:4907
UCSCiuc003pko.5 human [P21589-1]

Organism-specific databases

CTDi4907
DisGeNETi4907
EuPathDBiHostDB:ENSG00000135318.11
GeneCardsiNT5E
HGNCiHGNC:8021 NT5E
HPAiHPA017357
MalaCardsiNT5E
MIMi129190 gene
211800 phenotype
neXtProtiNX_P21589
OpenTargetsiENSG00000135318
Orphaneti289601 Hereditary arterial and articular multiple calcification syndrome
PharmGKBiPA31804
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4419 Eukaryota
COG0737 LUCA
GeneTreeiENSGT00530000063775
HOGENOMiHOG000247215
HOVERGENiHBG000026
KOiK19970
OMAiKRLFKPY
OrthoDBiEOG091G08IK
PhylomeDBiP21589
TreeFamiTF323589

Enzyme and pathway databases

BioCyciMetaCyc:HS05981-MONOMER
ReactomeiR-HSA-196807 Nicotinate metabolism
R-HSA-73621 Pyrimidine catabolism
R-HSA-74259 Purine catabolism
SIGNORiP21589

Miscellaneous databases

ChiTaRSiNT5E human
GeneWikiiNT5E
GenomeRNAii4907
PROiPR:P21589
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135318 Expressed in 220 organ(s), highest expression level in endometrium
CleanExiHS_NT5E
ExpressionAtlasiP21589 baseline and differential
GenevisibleiP21589 HS

Family and domain databases

Gene3Di3.60.21.10, 1 hit
3.90.780.10, 1 hit
InterProiView protein in InterPro
IPR008334 5'-Nucleotdase_C
IPR036907 5'-Nucleotdase_C_sf
IPR006146 5'-Nucleotdase_CS
IPR006179 5_nucleotidase/apyrase
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
PANTHERiPTHR11575 PTHR11575, 1 hit
PfamiView protein in Pfam
PF02872 5_nucleotid_C, 1 hit
PF00149 Metallophos, 1 hit
PRINTSiPR01607 APYRASEFAMLY
SUPFAMiSSF55816 SSF55816, 1 hit
PROSITEiView protein in PROSITE
PS00785 5_NUCLEOTIDASE_1, 1 hit
PS00786 5_NUCLEOTIDASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5NTD_HUMAN
AccessioniPrimary (citable) accession number: P21589
Secondary accession number(s): B3KQI8, O75520, Q5W116
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 7, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  7. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
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