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Protein

5'-nucleotidase

Gene

Nt5e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Zinc 1By similarity1
Metal bindingi40Zinc 1By similarity1
Metal bindingi87Zinc 1By similarity1
Metal bindingi87Zinc 2By similarity1
Metal bindingi119Zinc 2By similarity1
Sitei120Transition state stabilizerBy similarity1
Sitei123Transition state stabilizerBy similarity1
Metal bindingi222Zinc 2By similarity1
Metal bindingi245Zinc 2By similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei356SubstrateBy similarity1
Binding sitei392SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1
Binding sitei419SubstrateBy similarity1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: CACAO
  • ferrous iron binding Source: RGD
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • adenosine metabolic process Source: RGD
  • AMP catabolic process Source: RGD
  • brain development Source: RGD
  • positive regulation of lipid biosynthetic process Source: RGD
  • purine nucleotide biosynthetic process Source: RGD
  • response to aluminum ion Source: RGD

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Alternative name(s):
Ecto-5'-nucleotidase
CD_antigen: CD73
Gene namesi
Name:Nt5e
Synonyms:Nt5, Nte
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61956 Nt5e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075214
GuidetoPHARMACOLOGYi1232

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Add BLAST28
ChainiPRO_000000001929 – 5515'-nucleotidaseAdd BLAST523
PropeptideiPRO_0000000020552 – 576Removed in mature formAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 59By similarity
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi313N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi335N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi355 ↔ 360By similarity
Disulfide bondi367 ↔ 389By similarity
Glycosylationi405N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi478 ↔ 481By similarity
Lipidationi551GPI-anchor amidated serine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP21588
PRIDEiP21588

PTM databases

iPTMnetiP21588
PhosphoSitePlusiP21588

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015057

Chemistry databases

BindingDBiP21588

Structurei

3D structure databases

ProteinModelPortaliP21588
SMRiP21588
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni502 – 508Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4419 Eukaryota
COG0737 LUCA
HOGENOMiHOG000247215
HOVERGENiHBG000026
InParanoidiP21588
PhylomeDBiP21588

Family and domain databases

Gene3Di3.60.21.10, 1 hit
3.90.780.10, 1 hit
InterProiView protein in InterPro
IPR008334 5'-Nucleotdase_C
IPR036907 5'-Nucleotdase_C_sf
IPR006146 5'-Nucleotdase_CS
IPR006179 5_nucleotidase/apyrase
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
PANTHERiPTHR11575 PTHR11575, 1 hit
PfamiView protein in Pfam
PF02872 5_nucleotid_C, 1 hit
PF00149 Metallophos, 1 hit
PRINTSiPR01607 APYRASEFAMLY
SUPFAMiSSF55816 SSF55816, 1 hit
PROSITEiView protein in PROSITE
PS00785 5_NUCLEOTIDASE_1, 1 hit
PS00786 5_NUCLEOTIDASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21588-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPAAATAPK WLLLALSALL PLWPTAKSWE LTIMHTNDVH SRLEQTSDDS
60 70 80 90 100
TKCLNASLCV GGVARLFTKV QQIRKEEPNV LLLDAGDQYQ GTIWFTVYKG
110 120 130 140 150
LEVAHFMNLL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG
160 170 180 190 200
PLAPQISGLY LPYKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEVT
210 220 230 240 250
ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDV VVGGHTNTFL
260 270 280 290 300
YTGNPPSKEV PAGKYPFIVT SDDGRKVPVV QAYAFGKYLG YLKVEFDDKG
310 320 330 340 350
NVVTSYGNPI LLNSTIREDA AIKADINQWR IKLDNYSTQE LGRTIVYLNG
360 370 380 390 400
SAQECRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI
410 420 430 440 450
DERNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE
460 470 480 490 500
FLQVGGIHVV YDISRKPWDR VVQLKVLCTK CRVPIYEPLE MDKVYKVVLP
510 520 530 540 550
SYLVNGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVI YPAVEGRIKF
560 570
SAASHYQGSF PLIILSFWAV ILVLYQ
Length:576
Mass (Da):63,969
Last modified:May 1, 1991 - v1
Checksum:i9EB75DD51E678AA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05214 mRNA Translation: AAA40621.1
PIRiA35036
UniGeneiRn.40132

Genome annotation databases

UCSCiRGD:61956 rat

Similar proteinsi

Entry informationi

Entry namei5NTD_RAT
AccessioniPrimary (citable) accession number: P21588
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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