UniProtKB - P21552 (WNT2_MOUSE)
Protein
Protein Wnt-2
Gene
Wnt2
Organism
Mus musculus (Mouse)
Status
Functioni
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:19686689). Functions as upstream regulator of FGF10 expression (PubMed:19686689). Plays an important role in embryonic lung development (PubMed:19686689). May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (PubMed:20018874).Curated1 Publication
GO - Molecular functioni
- cytokine activity Source: MGI
- frizzled binding Source: MGI
- receptor ligand activity Source: MGI
GO - Biological processi
- atrial cardiac muscle tissue morphogenesis Source: MGI
- canonical Wnt signaling pathway Source: MGI
- canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation Source: MGI
- cardiac epithelial to mesenchymal transition Source: MGI
- cell-cell signaling Source: MGI
- cell fate commitment Source: GO_Central
- cell proliferation in midbrain Source: CACAO
- cellular response to transforming growth factor beta stimulus Source: Ensembl
- labyrinthine layer blood vessel development Source: MGI
- lung development Source: Ensembl
- lung induction Source: MGI
- mammary gland epithelium development Source: Ensembl
- midbrain dopaminergic neuron differentiation Source: MGI
- neuron differentiation Source: GO_Central
- positive regulation of cardiac muscle cell proliferation Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of endothelial cell proliferation Source: MGI
- positive regulation of epithelial cell proliferation involved in lung morphogenesis Source: MGI
- positive regulation of fibroblast proliferation Source: MGI
- positive regulation of mesenchymal cell proliferation Source: MGI
- positive regulation of neurogenesis Source: CACAO
- positive regulation of transcription by RNA polymerase II Source: MGI
- Wnt signaling pathway Source: MGI
Keywordsi
Molecular function | Developmental protein |
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-MMU-3238698, WNT ligand biogenesis and trafficking |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Wnt-2Alternative name(s): INT-1-related protein1 Publication Short name: IRP |
Gene namesi | Name:Wnt2 Synonyms:Irp, Wnt-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98954, Wnt2 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
- Secreted By similarity
Extracellular region or secreted
- collagen-containing extracellular matrix Source: MGI
- extracellular space Source: GO_Central
Plasma Membrane
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPathology & Biotechi
Disruption phenotypei
Nearly complete perinatal lethality within minutes after birth, due to lung hypoplasia (PubMed:19686689). About 4% survive for more than 30 days (PubMed:19686689). Combined disruption of Wnt2 and Wnt2b leads to lung agenesis and loss of trachea development (PubMed:19686689). In contrast, development of liver, stomach, intestine, pancreas and kidneys appears grossly normal (PubMed:19686689).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000041411 | 26 – 360 | Protein Wnt-2Add BLAST | 335 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 76 ↔ 87 | By similarity | ||
Disulfide bondi | 127 ↔ 135 | By similarity | ||
Disulfide bondi | 137 ↔ 157 | By similarity | ||
Disulfide bondi | 206 ↔ 220 | By similarity | ||
Disulfide bondi | 208 ↔ 215 | By similarity | ||
Lipidationi | 212 | O-palmitoleoyl serine; by PORCNBy similarity | 1 | |
Disulfide bondi | 278 ↔ 309 | By similarity | ||
Disulfide bondi | 294 ↔ 304 | By similarity | ||
Glycosylationi | 295 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 308 ↔ 348 | By similarity | ||
Disulfide bondi | 324 ↔ 339 | By similarity | ||
Disulfide bondi | 326 ↔ 336 | By similarity | ||
Disulfide bondi | 331 ↔ 332 | By similarity |
Post-translational modificationi
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, LipoproteinProteomic databases
MaxQBi | P21552 |
PaxDbi | P21552 |
PRIDEi | P21552 |
PTM databases
GlyGeni | P21552, 1 site |
iPTMneti | P21552 |
PhosphoSitePlusi | P21552 |
Expressioni
Tissue specificityi
In embryos in the developing allantois, pericardium heart, and ventral-lateral mesoderm; in adults in lung, brain, heart and placenta.
Developmental stagei
Detected in ventral mesencephalon from 10.5 to 15.5 dpc; expression levels decrease moderately, but steadily during this period (PubMed:20018874). Detected in the lateral plate mesoderm surrounding the ventral aspect of the anterior foregut from 9.0 to 10.5 dpc (PubMed:19686689). Detected in the developing mesenchyme with higher levels surrounding the distal regions of the branching airways from 12.5 to 18.5 dpc (PubMed:19686689).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000010797, Expressed in lung and 169 other tissues |
Genevisiblei | P21552, MM |
Interactioni
GO - Molecular functioni
- cytokine activity Source: MGI
- frizzled binding Source: MGI
- receptor ligand activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 204572, 2 interactors |
STRINGi | 10090.ENSMUSP00000010941 |
Chemistry databases
BindingDBi | P21552 |
Miscellaneous databases
RNActi | P21552, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the Wnt family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3913, Eukaryota |
GeneTreei | ENSGT00940000159231 |
HOGENOMi | CLU_033039_1_4_1 |
InParanoidi | P21552 |
OMAi | ITRMTKC |
OrthoDBi | 745245at2759 |
TreeFami | TF105310 |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR009140, Wnt2 IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01842, WNT2PROTEIN PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P21552-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNVPLGGIWL WLPLLLTWLT PEVSSSWWYM RATGGSSRVM CDNVPGLVSR
60 70 80 90 100
QRQLCHRHPD VMRAIGLGVA EWTAECQHQF RQHRWNCNTL DRDHSLFGRV
110 120 130 140 150
LLRSSRESAF VYAISSAGVV FAITRACSQG ELKSCSCDPK KKGSAKDSKG
160 170 180 190 200
TFDWGGCSDN IDYGIKFARA FVDAKERKGK DARALMNLHN NRAGRKAVKR
210 220 230 240 250
FLKQECKCHG VSGSCTLRTC WLAMADFRKT GDYLWRKYNG AIQVVMNQDG
260 270 280 290 300
TGFTVANKRF KKPTKNDLVY FENSPDYCIR DREAGSLGTA GRVCNLTSRG
310 320 330 340 350
MDSCEVMCCG RGYDTSHVTR MTKCECKFHW CCAVRCQDCL EALDVHTCKA
360
PKSADWATPT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 232 | D → E no nucleotide entry (PubMed:2693041).Curated | 1 | |
Sequence conflicti | 232 | D → E no nucleotide entry (PubMed:2279700).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK012093 mRNA Translation: BAB28025.1 CH466533 Genomic DNA Translation: EDL13868.1 BC026373 mRNA Translation: AAH26373.1 |
CCDSi | CCDS19928.1 |
PIRi | B36470 |
RefSeqi | NP_076142.3, NM_023653.5 |
Genome annotation databases
Ensembli | ENSMUST00000010941; ENSMUSP00000010941; ENSMUSG00000010797 |
GeneIDi | 22413 |
KEGGi | mmu:22413 |
UCSCi | uc009bag.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK012093 mRNA Translation: BAB28025.1 CH466533 Genomic DNA Translation: EDL13868.1 BC026373 mRNA Translation: AAH26373.1 |
CCDSi | CCDS19928.1 |
PIRi | B36470 |
RefSeqi | NP_076142.3, NM_023653.5 |
3D structure databases
SMRi | P21552 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204572, 2 interactors |
STRINGi | 10090.ENSMUSP00000010941 |
Chemistry databases
BindingDBi | P21552 |
PTM databases
GlyGeni | P21552, 1 site |
iPTMneti | P21552 |
PhosphoSitePlusi | P21552 |
Proteomic databases
MaxQBi | P21552 |
PaxDbi | P21552 |
PRIDEi | P21552 |
Protocols and materials databases
Antibodypediai | 4474, 268 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000010941; ENSMUSP00000010941; ENSMUSG00000010797 |
GeneIDi | 22413 |
KEGGi | mmu:22413 |
UCSCi | uc009bag.2, mouse |
Organism-specific databases
CTDi | 7472 |
MGIi | MGI:98954, Wnt2 |
Phylogenomic databases
eggNOGi | KOG3913, Eukaryota |
GeneTreei | ENSGT00940000159231 |
HOGENOMi | CLU_033039_1_4_1 |
InParanoidi | P21552 |
OMAi | ITRMTKC |
OrthoDBi | 745245at2759 |
TreeFami | TF105310 |
Enzyme and pathway databases
Reactomei | R-MMU-3238698, WNT ligand biogenesis and trafficking |
Miscellaneous databases
BioGRID-ORCSi | 22413, 1 hit in 17 CRISPR screens |
PROi | PR:P21552 |
RNActi | P21552, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010797, Expressed in lung and 169 other tissues |
Genevisiblei | P21552, MM |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR009140, Wnt2 IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01842, WNT2PROTEIN PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WNT2_MOUSE | |
Accessioni | P21552Primary (citable) accession number: P21552 Secondary accession number(s): Q9CZW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families