UniProtKB - P21538 (REB1_YEAST)
DNA-binding protein REB1
REB1
Functioni
DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromatin to a promoter.
2 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 497 – 519 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor activity Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific DNA binding Source: UniProtKB
- transcription cis-regulatory region binding Source: GO_Central
GO - Biological processi
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- regulation of transcription by RNA polymerase II Source: SGD
- termination of RNA polymerase II transcription Source: SGD
- termination of RNA polymerase I transcription Source: SGD
- transcriptional start site selection at RNA polymerase II promoter Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding protein REB1Alternative name(s): QBP |
Gene namesi | Name:REB1 Synonyms:GRF2 Ordered Locus Names:YBR049C ORF Names:YBR0502 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000253, REB1 |
VEuPathDBi | FungiDB:YBR049C |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: SGD
- nucleus Source: GO_Central
Other locations
- chromatin Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197089 | 1 – 810 | DNA-binding protein REB1Add BLAST | 810 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 355 | PhosphoserineCombined sources | 1 | |
Cross-linki | 807 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P21538 |
PaxDbi | P21538 |
PRIDEi | P21538 |
PTM databases
iPTMneti | P21538 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32751, 632 interactors |
DIPi | DIP-6374N |
IntActi | P21538, 33 interactors |
MINTi | P21538 |
STRINGi | 4932.YBR049C |
Miscellaneous databases
RNActi | P21538, protein |
Structurei
3D structure databases
AlphaFoldDBi | P21538 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 470 – 523 | HTH myb-typePROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 692 – 717 | Myb-likePROSITE-ProRule annotationAdd BLAST | 26 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 80 | DisorderedSequence analysisAdd BLAST | 80 | |
Regioni | 114 – 161 | DisorderedSequence analysisAdd BLAST | 48 | |
Regioni | 180 – 243 | DisorderedSequence analysisAdd BLAST | 64 | |
Regioni | 294 – 313 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 346 – 365 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 572 – 667 | DisorderedSequence analysisAdd BLAST | 96 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 32 – 52 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 141 – 160 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 192 – 224 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 294 – 312 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 640 – 666 | Polar residuesSequence analysisAdd BLAST | 27 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0051, Eukaryota |
GeneTreei | ENSGT00940000176744 |
HOGENOMi | CLU_016706_0_0_1 |
InParanoidi | P21538 |
OMAi | HVRRKYH |
Family and domain databases
CDDi | cd00167, SANT, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR001005, SANT/Myb |
SMARTi | View protein in SMART SM00717, SANT, 4 hits |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51294, HTH_MYB, 1 hit PS50090, MYB_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN
60 70 80 90 100
IVESSSDVDV NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH
110 120 130 140 150
NAVMDWYLRQ TAHNQQDDED DENNNNTDNG NDSNNHFSQS DIVVDDDDDK
160 170 180 190 200
NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN NNNNNSIAND SNSRKRQHDN
210 220 230 240 250
GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT NNDVIDRDQL
260 270 280 290 300
VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN
310 320 330 340 350
KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP
360 370 380 390 400
HQQKSPSHDN EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN
410 420 430 440 450
NSKAFDKAED AALERFINEY EAIERLTRQQ VCERIWSSDR PKDNFWNNIY
460 470 480 490 500
KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA EEEQELAKLC AEKEGQWAEI
510 520 530 540 550
GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK VISDMLEEAQ
560 570 580 590 600
QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ
610 620 630 640 650
QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM
660 670 680 690 700
VPAPSATSTH SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM
710 720 730 740 750
GGTRSRIQCR YKWNKLVKRE AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ
760 770 780 790 800
VDWDELAALK PGMKLNGLEL KLCYERMKKK VKGYKQKSIN EISKELVDYF
810
SSNISMKTEN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 56 | S → N in AAA34963 (PubMed:2204808).Curated | 1 | |
Sequence conflicti | 592 – 618 | AAAAI…IAAAA → RAVVFKNNNNFFNKSSKMMT MLLRSC in AAA34963 (PubMed:2204808).CuratedAdd BLAST | 27 | |
Sequence conflicti | 627 | D → E in AAA34963 (PubMed:2204808).Curated | 1 | |
Sequence conflicti | 636 | D → E in AAA34963 (PubMed:2204808).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58728 Genomic DNA Translation: AAA34963.1 Z35918 Genomic DNA Translation: CAA84992.1 Z46260 Genomic DNA Translation: CAA86391.1 BK006936 Genomic DNA Translation: DAA07169.1 |
PIRi | S45907 |
RefSeqi | NP_009605.1, NM_001178397.1 |
Genome annotation databases
EnsemblFungii | YBR049C_mRNA; YBR049C; YBR049C |
GeneIDi | 852338 |
KEGGi | sce:YBR049C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58728 Genomic DNA Translation: AAA34963.1 Z35918 Genomic DNA Translation: CAA84992.1 Z46260 Genomic DNA Translation: CAA86391.1 BK006936 Genomic DNA Translation: DAA07169.1 |
PIRi | S45907 |
RefSeqi | NP_009605.1, NM_001178397.1 |
3D structure databases
AlphaFoldDBi | P21538 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 32751, 632 interactors |
DIPi | DIP-6374N |
IntActi | P21538, 33 interactors |
MINTi | P21538 |
STRINGi | 4932.YBR049C |
PTM databases
iPTMneti | P21538 |
Proteomic databases
MaxQBi | P21538 |
PaxDbi | P21538 |
PRIDEi | P21538 |
Genome annotation databases
EnsemblFungii | YBR049C_mRNA; YBR049C; YBR049C |
GeneIDi | 852338 |
KEGGi | sce:YBR049C |
Organism-specific databases
SGDi | S000000253, REB1 |
VEuPathDBi | FungiDB:YBR049C |
Phylogenomic databases
eggNOGi | KOG0051, Eukaryota |
GeneTreei | ENSGT00940000176744 |
HOGENOMi | CLU_016706_0_0_1 |
InParanoidi | P21538 |
OMAi | HVRRKYH |
Miscellaneous databases
PROi | PR:P21538 |
RNActi | P21538, protein |
Family and domain databases
CDDi | cd00167, SANT, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR001005, SANT/Myb |
SMARTi | View protein in SMART SM00717, SANT, 4 hits |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51294, HTH_MYB, 1 hit PS50090, MYB_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | REB1_YEAST | |
Accessioni | P21538Primary (citable) accession number: P21538 Secondary accession number(s): D6VQ49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names