REB1

Functionⁱ

DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromotin to a promoter.2 Publications

Miscellaneous

Present with 7510 molecules/cell in log phase SD medium.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	497 – 519	H-T-H motifPROSITE-ProRule annotationAdd BLAST		23

GO - Molecular functionⁱ

sequence-specific DNA binding
Source: SGD
Inferred from direct assayⁱ
- 25479637
- 27601258

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

regulation of transcription by RNA polymerase II
Source: SGD
Inferred from mutant phenotypeⁱ
- 27601258
termination of RNA polymerase II transcription
Source: SGD
Inferred from mutant phenotypeⁱ
- 25479637
termination of RNA polymerase I transcription
Source: SGD
Traceable author statementⁱ
- 7565745
transcriptional start site selection at RNA polymerase II promoter
Source: SGD
Inferred from direct assayⁱ
- 27601258

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29021-MONOMER

BioCycⁱ

YEAST:G3O-29021-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA-binding protein REB1 Alternative name(s): QBP
Gene namesⁱ	Name:REB1 Synonyms:GRF2 Ordered Locus Names:YBR049C ORF Names:YBR0502
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome II

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000000253 REB1

SGDⁱ

S000000253 REB1

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000197089	1 – 810	DNA-binding protein REB1Add BLAST		810

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	355	PhosphoserineCombined sources		1
Cross-linkⁱ	807	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P21538
PaxDbⁱ	P21538
PRIDEⁱ	P21538

PTM databases

iPTMnetⁱ	P21538

iPTMnetⁱ

P21538

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	32751, 628 interactors
Database of interacting proteins More...DIPⁱ	DIP-6374N
IntActⁱ	P21538, 33 interactors
Molecular INTeraction database More...MINTⁱ	P21538
STRINGⁱ	4932.YBR049C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P21538
SMRⁱ	P21538
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	470 – 523	HTH myb-typePROSITE-ProRule annotationAdd BLAST		54
Domainⁱ	692 – 717	Myb-likePROSITE-ProRule annotationAdd BLAST		26

Keywords - Domainⁱ

Repeat

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00720000110079
InParanoidⁱ	P21538
KEGG Orthology (KO) More...KOⁱ	K09424
OMAⁱ	HANSNEQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C01T9

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00167 SANT, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR017877 Myb-like_dom IPR015495 Myb-like_TF IPR017930 Myb_dom IPR001005 SANT/Myb
PANTHERⁱ	PTHR10641 PTHR10641, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00249 Myb_DNA-binding, 1 hit
SMARTⁱ	View protein in SMART SM00717 SANT, 4 hits
SUPFAMⁱ	SSF46689 SSF46689, 2 hits
PROSITEⁱ	View protein in PROSITE PS51294 HTH_MYB, 1 hit PS50090 MYB_LIKE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P21538-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN 
        60         70         80         90        100
IVESSSDVDV NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH 
       110        120        130        140        150
NAVMDWYLRQ TAHNQQDDED DENNNNTDNG NDSNNHFSQS DIVVDDDDDK 
       160        170        180        190        200
NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN NNNNNSIAND SNSRKRQHDN 
       210        220        230        240        250
GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT NNDVIDRDQL 
       260        270        280        290        300
VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN 
       310        320        330        340        350
KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP 
       360        370        380        390        400
HQQKSPSHDN EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN 
       410        420        430        440        450
NSKAFDKAED AALERFINEY EAIERLTRQQ VCERIWSSDR PKDNFWNNIY 
       460        470        480        490        500
KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA EEEQELAKLC AEKEGQWAEI 
       510        520        530        540        550
GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK VISDMLEEAQ 
       560        570        580        590        600
QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ 
       610        620        630        640        650
QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM 
       660        670        680        690        700
VPAPSATSTH SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM 
       710        720        730        740        750
GGTRSRIQCR YKWNKLVKRE AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ 
       760        770        780        790        800
VDWDELAALK PGMKLNGLEL KLCYERMKKK VKGYKQKSIN EISKELVDYF 
       810
SSNISMKTEN

Length:810

Mass (Da):91,874

Last modified:October 1, 1994 - v2

Checksum:ⁱ318EB17D20D004B6

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	56	S → N in AAA34963 (PubMed:2204808).Curated	1
Sequence conflictⁱ	592 – 618	AAAAI…IAAAA → RAVVFKNNNNFFNKSSKMMT MLLRSC in AAA34963 (PubMed:2204808).CuratedAdd BLAST	27
Sequence conflictⁱ	627	D → E in AAA34963 (PubMed:2204808).Curated	1
Sequence conflictⁱ	636	D → E in AAA34963 (PubMed:2204808).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M58728 Genomic DNA Translation: AAA34963.1 Z35918 Genomic DNA Translation: CAA84992.1 Z46260 Genomic DNA Translation: CAA86391.1 BK006936 Genomic DNA Translation: DAA07169.1
PIRⁱ	S45907
NCBI Reference Sequences More...RefSeqⁱ	NP_009605.1, NM_001178397.1

Genome annotation databases

EnsemblFungiⁱ	YBR049C; YBR049C; YBR049C
GeneIDⁱ	852338
KEGGⁱ	sce:YBR049C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P21538	RNA polymerase I enhancer binding protein		YEAS1		810	UniRef100_P21538
K7_Reb1p		YEASK		810
Reb1p		YEASC		810
Reb1p		YEAS2		810
YBR049Cp-like protein		YEAS6		266

Protein	Similar proteins		Species	Score	Length	Source
P21538	RNA polymerase I enhancer binding protein		YEAS1		810	UniRef90_P21538
K7_Reb1p		YEASK		810
Reb1p		YEASC		810
Reb1p		YEAS2		810
YBR049Cp-like protein		YEAS6		266
+5

Protein	Similar proteins		Species	Score	Length	Source
P21538	RNA polymerase I enhancer binding protein		YEAS1		810	UniRef50_P21538
K7_Reb1p		YEASK		810
Reb1p		YEASC		810
Reb1p		YEAS2		810
YBR049Cp-like protein		YEAS6		266
+7

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M58728 Genomic DNA Translation: AAA34963.1 Z35918 Genomic DNA Translation: CAA84992.1 Z46260 Genomic DNA Translation: CAA86391.1 BK006936 Genomic DNA Translation: DAA07169.1
PIRⁱ	S45907
RefSeqⁱ	NP_009605.1, NM_001178397.1

3D structure databases

ProteinModelPortalⁱ	P21538
SMRⁱ	P21538
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32751, 628 interactors
DIPⁱ	DIP-6374N
IntActⁱ	P21538, 33 interactors
MINTⁱ	P21538
STRINGⁱ	4932.YBR049C

PTM databases

iPTMnetⁱ	P21538

iPTMnetⁱ

P21538

Proteomic databases

MaxQBⁱ	P21538
PaxDbⁱ	P21538
PRIDEⁱ	P21538

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YBR049C; YBR049C; YBR049C
GeneIDⁱ	852338
KEGGⁱ	sce:YBR049C

Organism-specific databases

SGDⁱ	S000000253 REB1

Phylogenomic databases

GeneTreeⁱ	ENSGT00720000110079
InParanoidⁱ	P21538
KOⁱ	K09424
OMAⁱ	HANSNEQ
OrthoDBⁱ	EOG092C01T9

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29021-MONOMER

BioCycⁱ

YEAST:G3O-29021-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P21538

PROⁱ

PR:P21538

Family and domain databases

CDDⁱ	cd00167 SANT, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR017877 Myb-like_dom IPR015495 Myb-like_TF IPR017930 Myb_dom IPR001005 SANT/Myb
PANTHERⁱ	PTHR10641 PTHR10641, 1 hit
Pfamⁱ	View protein in Pfam PF00249 Myb_DNA-binding, 1 hit
SMARTⁱ	View protein in SMART SM00717 SANT, 4 hits
SUPFAMⁱ	SSF46689 SSF46689, 2 hits
PROSITEⁱ	View protein in PROSITE PS51294 HTH_MYB, 1 hit PS50090 MYB_LIKE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	REB1_YEAST
Accessionⁱ	P21538Primary (citable) accession number: P21538 Secondary accession number(s): D6VQ49
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
	Last sequence update:	October 1, 1994
	Last modified:	November 7, 2018
	This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P21538 (REB1_YEAST)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

DNA-binding protein REB1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ