UniProtKB - P21517 (MALZ_ECOLI)
Protein
Maltodextrin glucosidase
Gene
malZ
Organism
Escherichia coli (strain K12)
Status
Functioni
May play a role in regulating the intracellular level of maltotriose. Cleaves glucose from the reducing end of maltotriose and longer maltodextrins with a chain length of up to 7 glucose units.
Catalytic activityi
- Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose. EC:3.2.1.20
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 336 | NucleophileBy similarity | 1 | |
Active sitei | 373 | Proton donorBy similarity | 1 | |
Sitei | 448 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- alpha-1,4-glucosidase activity Source: EcoCyc
- maltose alpha-glucosidase activity Source: UniProtKB-EC
GO - Biological processi
- alpha-glucan catabolic process Source: EcoCyc
- cellular oligosaccharide catabolic process Source: EcoCyc
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Maltose metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:MALTODEXGLUCOSID-MONOMER MetaCyc:MALTODEXGLUCOSID-MONOMER |
BRENDAi | 3.2.1.20, 2026 |
Protein family/group databases
CAZyi | CBM34, Carbohydrate-Binding Module Family 34 GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:malZ Ordered Locus Names:b0403, JW0393 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054334 | 1 – 604 | Maltodextrin glucosidaseAdd BLAST | 604 |
Proteomic databases
jPOSTi | P21517 |
PaxDbi | P21517 |
PRIDEi | P21517 |
Expressioni
Inductioni
Under positive control of MalT.
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4263268, 9 interactors |
DIPi | DIP-10152N |
IntActi | P21517, 8 interactors |
MINTi | P21517 |
STRINGi | 511145.b0403 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
HOGENOMi | CLU_006462_6_4_6 |
InParanoidi | P21517 |
Family and domain databases
CDDi | cd02857, E_set_CDase_PDE_N, 1 hit |
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006047, Glyco_hydro_13_cat_dom IPR004185, Glyco_hydro_13_lg-like_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR014756, Ig_E-set IPR017069, MalZ |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02903, Alpha-amylase_N, 1 hit |
PIRSFi | PIRSF036918, Maltodextrin_glucosidase, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF81296, SSF81296, 1 hit |
i Sequence
Sequence statusi: Complete.
P21517-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNAWHLPVP PFVKQSKDQL LITLWLTGED PPQRIMLRTE HDNEEMSVPM
60 70 80 90 100
HKQRSQPQPG VTAWRAAIDL SSGQPRRRYS FKLLWHDRQR WFTPQGFSRM
110 120 130 140 150
PPARLEQFAV DVPDIGPQWA ADQIFYQIFP DRFARSLPRE AEQDHVYYHH
160 170 180 190 200
AAGQEIILRD WDEPVTAQAG GSTFYGGDLD GISEKLPYLK KLGVTALYLN
210 220 230 240 250
PVFKAPSVHK YDTEDYRHVD PQFGGDGALL RLRHNTQQLG MRLVLDGVFN
260 270 280 290 300
HSGDSHAWFD RHNRGTGGAC HNPESPWRDW YSFSDDGTAL DWLGYASLPK
310 320 330 340 350
LDYQSESLVN EIYRGEDSIV RHWLKAPWNM DGWRLDVVHM LGEAGGARNN
360 370 380 390 400
MQHVAGITEA AKETQPEAYI VGEHFGDARQ WLQADVEDAA MNYRGFTFPL
410 420 430 440 450
WGFLANTDIS YDPQQIDAQT CMAWMDNYRA GLSHQQQLRM FNQLDSHDTA
460 470 480 490 500
RFKTLLGRDI ARLPLAVVWL FTWPGVPCIY YGDEVGLDGK NDPFCRKPFP
510 520 530 540 550
WQVEKQDTAL FALYQRMIAL RKKSQALRHG GCQVLYAEDN VVVFVRVLNQ
560 570 580 590 600
QRVLVAINRG EACEVVLPAS PFLNAVQWQC KEGHGQLTDG ILALPAISAT
VWMN
Sequence cautioni
The sequence AAB40159 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAE76183 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 301 | L → M in CAA42498 (PubMed:1918057).Curated | 1 | |
Sequence conflicti | 445 | D → E in CAA42498 (PubMed:1918057).Curated | 1 | |
Sequence conflicti | 546 | R → S in CAA42498 (PubMed:1918057).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59839 Genomic DNA Translation: CAA42498.1 U82664 Genomic DNA Translation: AAB40159.1 Different initiation. U00096 Genomic DNA Translation: AAC73506.2 AP009048 Genomic DNA Translation: BAE76183.1 Different initiation. M37702 Genomic DNA Translation: AAA16112.1 |
PIRi | C64769 |
RefSeqi | NP_414937.2, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC73506; AAC73506; b0403 BAE76183; BAE76183; BAE76183 |
GeneIDi | 949131 |
KEGGi | ecj:JW0393 eco:b0403 |
PATRICi | fig|511145.12.peg.419 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59839 Genomic DNA Translation: CAA42498.1 U82664 Genomic DNA Translation: AAB40159.1 Different initiation. U00096 Genomic DNA Translation: AAC73506.2 AP009048 Genomic DNA Translation: BAE76183.1 Different initiation. M37702 Genomic DNA Translation: AAA16112.1 |
PIRi | C64769 |
RefSeqi | NP_414937.2, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5BN7 | X-ray | 3.70 | A | 1-604 | [»] | |
SMRi | P21517 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263268, 9 interactors |
DIPi | DIP-10152N |
IntActi | P21517, 8 interactors |
MINTi | P21517 |
STRINGi | 511145.b0403 |
Protein family/group databases
CAZyi | CBM34, Carbohydrate-Binding Module Family 34 GH13, Glycoside Hydrolase Family 13 |
Proteomic databases
jPOSTi | P21517 |
PaxDbi | P21517 |
PRIDEi | P21517 |
Genome annotation databases
EnsemblBacteriai | AAC73506; AAC73506; b0403 BAE76183; BAE76183; BAE76183 |
GeneIDi | 949131 |
KEGGi | ecj:JW0393 eco:b0403 |
PATRICi | fig|511145.12.peg.419 |
Organism-specific databases
EchoBASEi | EB0560 |
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
HOGENOMi | CLU_006462_6_4_6 |
InParanoidi | P21517 |
Enzyme and pathway databases
BioCyci | EcoCyc:MALTODEXGLUCOSID-MONOMER MetaCyc:MALTODEXGLUCOSID-MONOMER |
BRENDAi | 3.2.1.20, 2026 |
Miscellaneous databases
PROi | PR:P21517 |
Family and domain databases
CDDi | cd02857, E_set_CDase_PDE_N, 1 hit |
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006047, Glyco_hydro_13_cat_dom IPR004185, Glyco_hydro_13_lg-like_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR014756, Ig_E-set IPR017069, MalZ |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02903, Alpha-amylase_N, 1 hit |
PIRSFi | PIRSF036918, Maltodextrin_glucosidase, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MALZ_ECOLI | |
Accessioni | P21517Primary (citable) accession number: P21517 Secondary accession number(s): Q2MC23 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | November 16, 2011 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries