UniProtKB - P21507 (SRMB_ECOLI)
Protein
ATP-dependent RNA helicase SrmB
Gene
srmB
Organism
Escherichia coli (strain K12)
Status
Functioni
DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit at low temperature. Exhibits RNA-stimulated ATP hydrolysis and RNA unwinding activity. Acts before DeaD.UniRule annotation3 Publications
Miscellaneous
Overexpression suppresses a mutant defective in 50S ribosomal subunit assembly.1 Publication
Catalytic activityi
- EC:3.6.4.13UniRule annotation1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 48 – 55 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: UniProtKB
- ATP binding Source: UniProtKB-UniRule
- poly(A) binding Source: UniProtKB
- RNA-dependent ATPase activity Source: UniProtKB
- RNA helicase activity Source: UniProtKB
- RNA strand annealing activity Source: EcoCyc
GO - Biological processi
- ribosomal large subunit assembly Source: EcoCyc
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10975-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:srmBUniRule annotation Synonyms:rbaB, rhlA Ordered Locus Names:b2576, JW2560 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene grow very poorly at 20 degrees Celsius, show a severe deficit of free 50S ribosomal subunits and accumulate an abnormal large ribosomal subunit. Disruption also impairs the processing of both 23S and 16S rRNAs.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055109 | 1 – 444 | ATP-dependent RNA helicase SrmBAdd BLAST | 444 |
Proteomic databases
jPOSTi | P21507 |
PaxDbi | P21507 |
PRIDEi | P21507 |
Interactioni
Subunit structurei
Interacts with the 50S ribosomal subunit.
Forms a complex with the 50S ribosomal proteins L4 and L24, and a region near the 5'-end of 23S rRNA.
UniRule annotation1 PublicationBinary interactionsi
Hide detailsP21507
With | #Exp. | IntAct |
---|---|---|
pnp [P05055] | 3 | EBI-546628,EBI-548080 |
rlmN [P36979] | 4 | EBI-546628,EBI-559071 |
rluB [P37765] | 6 | EBI-546628,EBI-561550 |
rne [P21513] | 3 | EBI-546628,EBI-549958 |
Protein-protein interaction databases
BioGRIDi | 4263366, 231 interactors 851393, 5 interactors |
DIPi | DIP-10920N |
IntActi | P21507, 34 interactors |
STRINGi | 511145.b2576 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P21507 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 209 | Helicase ATP-bindingUniRule annotationAdd BLAST | 175 | |
Domaini | 238 – 387 | Helicase C-terminalUniRule annotationAdd BLAST | 150 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 4 – 32 | Q motifAdd BLAST | 29 | |
Motifi | 157 – 160 | DEAD box | 4 |
Domaini
The C-terminal extension is not essential for ribosome assembly, but is important for the formation or the stability of the complex.1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0513, Bacteria |
HOGENOMi | CLU_003041_1_3_6 |
InParanoidi | P21507 |
PhylomeDBi | P21507 |
Family and domain databases
HAMAPi | MF_00967, DEAD_helicase_SrmB, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR028621, DEAD_helicase_SrmB IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
P21507-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVTTFSELE LDESLLEALQ DKGFTRPTAI QAAAIPPALD GRDVLGSAPT
60 70 80 90 100
GTGKTAAYLL PALQHLLDFP RKKSGPPRIL ILTPTRELAM QVSDHARELA
110 120 130 140 150
KHTHLDIATI TGGVAYMNHA EVFSENQDIV VATTGRLLQY IKEENFDCRA
160 170 180 190 200
VETLILDEAD RMLDMGFAQD IEHIAGETRW RKQTLLFSAT LEGDAIQDFA
210 220 230 240 250
ERLLEDPVEV SANPSTRERK KIHQWYYRAD DLEHKTALLV HLLKQPEATR
260 270 280 290 300
SIVFVRKRER VHELANWLRE AGINNCYLEG EMVQGKRNEA IKRLTEGRVN
310 320 330 340 350
VLVATDVAAR GIDIPDVSHV FNFDMPRSGD TYLHRIGRTA RAGRKGTAIS
360 370 380 390 400
LVEAHDHLLL GKVGRYIEEP IKARVIDELR PKTRAPSEKQ TGKPSKKVLA
410 420 430 440
KRAEKKKAKE KEKPRVKKRH RDTKNIGKRR KPSGTGVPPQ TTEE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14152 Genomic DNA Translation: CAA32364.1 D13169 Genomic DNA Translation: BAA02447.1 D64044 Genomic DNA Translation: BAA10922.1 U00096 Genomic DNA Translation: AAC75629.1 AP009048 Genomic DNA Translation: BAE76752.1 |
PIRi | G65035 |
RefSeqi | NP_417071.1, NC_000913.3 WP_000219203.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75629; AAC75629; b2576 BAE76752; BAE76752; BAE76752 |
GeneIDi | 947055 |
KEGGi | ecj:JW2560 eco:b2576 |
PATRICi | fig|1411691.4.peg.4158 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14152 Genomic DNA Translation: CAA32364.1 D13169 Genomic DNA Translation: BAA02447.1 D64044 Genomic DNA Translation: BAA10922.1 U00096 Genomic DNA Translation: AAC75629.1 AP009048 Genomic DNA Translation: BAE76752.1 |
PIRi | G65035 |
RefSeqi | NP_417071.1, NC_000913.3 WP_000219203.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YJT | X-ray | 2.90 | D | 219-388 | [»] | |
SMRi | P21507 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263366, 231 interactors 851393, 5 interactors |
DIPi | DIP-10920N |
IntActi | P21507, 34 interactors |
STRINGi | 511145.b2576 |
Proteomic databases
jPOSTi | P21507 |
PaxDbi | P21507 |
PRIDEi | P21507 |
Genome annotation databases
EnsemblBacteriai | AAC75629; AAC75629; b2576 BAE76752; BAE76752; BAE76752 |
GeneIDi | 947055 |
KEGGi | ecj:JW2560 eco:b2576 |
PATRICi | fig|1411691.4.peg.4158 |
Organism-specific databases
EchoBASEi | EB0968 |
Phylogenomic databases
eggNOGi | COG0513, Bacteria |
HOGENOMi | CLU_003041_1_3_6 |
InParanoidi | P21507 |
PhylomeDBi | P21507 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10975-MONOMER |
Miscellaneous databases
PROi | PR:P21507 |
Family and domain databases
HAMAPi | MF_00967, DEAD_helicase_SrmB, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR028621, DEAD_helicase_SrmB IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRMB_ECOLI | |
Accessioni | P21507Primary (citable) accession number: P21507 Secondary accession number(s): Q2MAF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families