UniProtKB - P21466 (RS4_BACSU)
30S ribosomal protein S4
rpsD
Functioni
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.
By similarityWith S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).
S4 represses its own expression; it is not know if this is at the level of translation or of mRNA stability.
GO - Molecular functioni
- rRNA binding Source: GO_Central
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- positive regulation of translational fidelity Source: GO_Central
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Repressor, Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU29660-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 30S ribosomal protein S4Short name: BS4 |
Gene namesi | Name:rpsD Ordered Locus Names:BSU29660 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000132339 | 2 – 200 | 30S ribosomal protein S4Add BLAST | 199 |
Proteomic databases
jPOSTi | P21466 |
PaxDbi | P21466 |
PRIDEi | P21466 |
Interactioni
Subunit structurei
Part of the 30S ribosomal subunit (PubMed:30126986). Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.
1 PublicationProtein-protein interaction databases
IntActi | P21466, 1 interactor |
MINTi | P21466 |
STRINGi | 224308.BSU29660 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 92 – 155 | S4 RNA-bindingAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 42 | DisorderedSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0522, Bacteria |
InParanoidi | P21466 |
OMAi | NVVFRMG |
PhylomeDBi | P21466 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit |
HAMAPi | MF_01306_B, Ribosomal_S4_B, 1 hit |
InterProi | View protein in InterPro IPR022801, Ribosomal_S4/S9 IPR001912, Ribosomal_S4/S9_N IPR005709, Ribosomal_S4_bac-type IPR018079, Ribosomal_S4_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
PANTHERi | PTHR11831, PTHR11831, 1 hit PTHR11831:SF4, PTHR11831:SF4, 1 hit |
Pfami | View protein in Pfam PF00163, Ribosomal_S4, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM01390, Ribosomal_S4, 1 hit SM00363, S4, 1 hit |
TIGRFAMsi | TIGR01017, rpsD_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00632, RIBOSOMAL_S4, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MARYTGPSWK LSRRLGISLS GTGKELEKRP YAPGPHGPGQ RKKLSEYGLQ
60 70 80 90 100
LQEKQKLRHM YGVNERQFRT LFDKAGKLAG KHGENFMILL DSRLDNVVYK
110 120 130 140 150
LGLARTRRQA RQLVNHGHIL VDGSRVDIPS YLVKPGQTIG VREKSRNLSI
160 170 180 190 200
IKESVEVNNF VPEYLTFDAE KLEGTFTRLP ERSELAPEIN EALIVEFYSR
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 46 | E → K in rpsD3; suppresses S12 mutation K55D. 1 Publication | 1 | |
Natural varianti | 75 – 78 | Missing in rpsD2; suppresses S12 mutation K55D. A ram mutation. 2 Publications | 4 | |
Natural varianti | 78 | L → LAGKL in rpsD1; suppresses S12 mutation K55D. A ram mutation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59358 Genomic DNA Translation: AAA22717.1 AF008220 Genomic DNA Translation: AAC00397.1 S45404 Genomic DNA Translation: AAB19387.1 AL009126 Genomic DNA Translation: CAB14944.1 M60889 Genomic DNA Translation: AAA22716.1 |
PIRi | A37146 |
RefSeqi | NP_390844.1, NC_000964.3 WP_003229304.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14944; CAB14944; BSU_29660 |
GeneIDi | 937319 |
KEGGi | bsu:BSU29660 |
PATRICi | fig|224308.179.peg.3223 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59358 Genomic DNA Translation: AAA22717.1 AF008220 Genomic DNA Translation: AAC00397.1 S45404 Genomic DNA Translation: AAB19387.1 AL009126 Genomic DNA Translation: CAB14944.1 M60889 Genomic DNA Translation: AAA22716.1 |
PIRi | A37146 |
RefSeqi | NP_390844.1, NC_000964.3 WP_003229304.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J9W | electron microscopy | 3.90 | AD | 1-200 | [»] | |
5NJT | electron microscopy | 3.80 | D | 2-200 | [»] | |
6HA1 | electron microscopy | 3.10 | d | 1-200 | [»] | |
6HA8 | electron microscopy | 3.50 | d | 1-200 | [»] | |
6HTQ | electron microscopy | 4.50 | d | 6-200 | [»] | |
SMRi | P21466 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P21466, 1 interactor |
MINTi | P21466 |
STRINGi | 224308.BSU29660 |
Proteomic databases
jPOSTi | P21466 |
PaxDbi | P21466 |
PRIDEi | P21466 |
Genome annotation databases
EnsemblBacteriai | CAB14944; CAB14944; BSU_29660 |
GeneIDi | 937319 |
KEGGi | bsu:BSU29660 |
PATRICi | fig|224308.179.peg.3223 |
Phylogenomic databases
eggNOGi | COG0522, Bacteria |
InParanoidi | P21466 |
OMAi | NVVFRMG |
PhylomeDBi | P21466 |
Enzyme and pathway databases
BioCyci | BSUB:BSU29660-MONOMER |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit |
HAMAPi | MF_01306_B, Ribosomal_S4_B, 1 hit |
InterProi | View protein in InterPro IPR022801, Ribosomal_S4/S9 IPR001912, Ribosomal_S4/S9_N IPR005709, Ribosomal_S4_bac-type IPR018079, Ribosomal_S4_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
PANTHERi | PTHR11831, PTHR11831, 1 hit PTHR11831:SF4, PTHR11831:SF4, 1 hit |
Pfami | View protein in Pfam PF00163, Ribosomal_S4, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM01390, Ribosomal_S4, 1 hit SM00363, S4, 1 hit |
TIGRFAMsi | TIGR01017, rpsD_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00632, RIBOSOMAL_S4, 1 hit PS50889, S4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RS4_BACSU | |
Accessioni | P21466Primary (citable) accession number: P21466 Secondary accession number(s): Q45662, Q53282 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families