UniProtKB - P21457 (RECO_BOVIN)
Recoverin
RCVRN
Functioni
Acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells (PubMed:1672047, PubMed:1672637).
Modulates light sensitivity of cone photoreceptor in dark and dim conditions (By similarity).
In response to high Ca2+ levels induced by low light levels, prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting GRK1-mediated phosphorylation of RHO/rhodopsin (PubMed:1672047, PubMed:1672637, PubMed:8392055, PubMed:16675451, PubMed:21299498, PubMed:12686556, PubMed:17015448).
Plays a role in scotopic vision/enhances vision in dim light by enhancing signal transfer between rod photoreceptors and rod bipolar cells (By similarity).
Improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light (By similarity).
By similarity7 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 74 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 76 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 78 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 80 | Calcium 1; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 85 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 110 | Calcium 2Combined sources8 Publications | 1 | |
Metal bindingi | 112 | Calcium 2Combined sources8 Publications | 1 | |
Metal bindingi | 114 | Calcium 2Combined sources7 Publications | 1 | |
Metal bindingi | 116 | Calcium 2; via carbonyl oxygenCombined sources8 Publications | 1 | |
Metal bindingi | 121 | Calcium 2Combined sources7 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 74 – 85 | 1; low affinity1 PublicationAdd BLAST | 12 | |
Calcium bindingi | 110 – 121 | 2; high affinity1 PublicationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: Ensembl
GO - Biological processi
- phototransduction Source: Ensembl
- regulation of calcium ion transport Source: Ensembl
- visual perception Source: UniProtKB-KW
Keywordsi
Biological process | Sensory transduction, Vision |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: RecoverinAlternative name(s): p26 |
Gene namesi | Name:RCVRN Synonyms:RCV1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000025088 |
VGNCi | VGNC:33838, RCVRN |
Subcellular locationi
Other locations
- Photoreceptor inner segment 1 Publication
- photoreceptor outer segment 4 Publications
- Photoreceptor outer segment membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
- Perikaryon By similarity
Note: Primarily expressed in the inner segments of light-adapted rod photoreceptors, approximately 10% of which translocates from photoreceptor outer segments upon light stimulation (By similarity). Targeting of myristoylated protein to rod photoreceptor outer segments is calcium dependent (PubMed:17015448).By similarity1 Publication
Cytosol
- cytosol Source: Reactome
Other locations
- membrane Source: UniProtKB-KW
- perikaryon Source: UniProtKB-SubCell
- photoreceptor inner segment Source: UniProtKB-SubCell
- photoreceptor outer segment Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projection, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 39 | C → A: Increases calcium binding affinity at EF-hand 3 domain; induces co-operative calcium binding in non-myristoylated protein. 1 Publication | 1 | |
Mutagenesisi | 39 | C → D: Reduces binding affinity for calcium at both EF-hand 2 and EF-hand 3 domains. Abolishes interaction with GRK1. 1 Publication | 1 | |
Mutagenesisi | 40 | P → A: Reduces calcium binding affinity. 1 Publication | 1 | |
Mutagenesisi | 85 | E → Q: Abolishes binding of calcium to EF-hand 2 domain. Abolishes calcium-dependent inhibition of GRK1. 2 Publications | 1 | |
Mutagenesisi | 153 | E → A: No effect on calcium binding to EF-hand 2 and EF-hand 3 domains. No effect on interaction with GRK1. 1 Publication | 1 | |
Mutagenesisi | 185 – 202 | Missing : Decrease in thermostability. 1 PublicationAdd BLAST | 18 | |
Mutagenesisi | 187 – 202 | Missing : Decrease in thermostability. 1 PublicationAdd BLAST | 16 | |
Mutagenesisi | 188 – 202 | Missing : Decrease in thermostability. 1 PublicationAdd BLAST | 15 | |
Mutagenesisi | 189 – 202 | Missing : Reduces calcium binding affinity. Reduces interaction with GRK1. Reduces inhibition of GRK1 activity. 1 PublicationAdd BLAST | 14 | |
Mutagenesisi | 190 | P → G: Reduces interaction with GRK1. 1 Publication | 1 | |
Mutagenesisi | 191 – 202 | Missing : Reduces calcium binding affinity to EF-hand 3 domain. Reduces interaction with GRK1. 2 PublicationsAdd BLAST | 12 | |
Mutagenesisi | 191 | Q → A: Reduces inhibition of GRK1 activity. 1 Publication | 1 | |
Mutagenesisi | 192 | K → A: Reduces interaction with GRK1. Reduces inhibition of GRK1 activity. 1 Publication | 1 | |
Mutagenesisi | 193 – 202 | Missing : Reduces calcium binding affinity. Reduces interaction with GRK1. 1 Publication | 10 | |
Mutagenesisi | 193 | V → G: Reduces interaction with GRK1. 1 Publication | 1 | |
Mutagenesisi | 197 – 202 | Missing : Reduces interaction with GRK1. 1 Publication | 6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed5 Publications | |||
ChainiPRO_0000073758 | 2 – 202 | RecoverinAdd BLAST | 201 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine3 Publications | 1 | |
Modified residuei | 39 | Cysteine sulfenic acid (-SOH)2 Publications | 1 | |
Disulfide bondi | 39 | Interchain, redox-active1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Lipoprotein, Myristate, OxidationProteomic databases
PaxDbi | P21457 |
PTM databases
iPTMneti | P21457 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSBTAG00000025088, Expressed in retina and 44 other tissues |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (PubMed:25772009). Homodimerization is caused by prolonged intense illumination (PubMed:25772009). May form a complex composed of RHO, GRK1 and RCVRN in a Ca2+-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (PubMed:17020884).
Interacts (via C-terminus) with GRK1 (via N-terminus); the interaction is Ca2+-dependent (PubMed:16675451, PubMed:21299498, PubMed:24189072, PubMed:26584024).
6 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 192 | Interaction with GRK11 Publication | 1 |
Binary interactionsi
P21457
With | #Exp. | IntAct |
---|---|---|
GRK1 [P28327] | 2 | EBI-8592784,EBI-7865560 |
Protein-protein interaction databases
ELMi | P21457 |
IntActi | P21457, 4 interactors |
MINTi | P21457 |
STRINGi | 9913.ENSBTAP00000034949 |
Structurei
Secondary structure
3D structure databases
BMRBi | P21457 |
SMRi | P21457 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21457 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 59 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 61 – 96 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 97 – 132 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 147 – 182 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 189 – 192 | Interaction with GRK11 Publication | 4 | |
Regioni | 191 – 202 | Modulates EF-hand 3 domain calcium binding affinity1 PublicationAdd BLAST | 12 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Redox-active center, RepeatPhylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000159441 |
HOGENOMi | CLU_072366_1_0_1 |
InParanoidi | P21457 |
OMAi | TQKLEWA |
OrthoDBi | 1271942at2759 |
TreeFami | TF300009 |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ
60 70 80 90 100
TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ
110 120 130 140 150
KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK
160 170 180 190 200
RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK
KL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | L → Q AA sequence (PubMed:1672637).Curated | 1 | |
Sequence conflicti | 21 | T → N AA sequence (PubMed:1672637).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95858 mRNA Translation: AAB59256.1 X63322 mRNA Translation: CAA44928.1 |
PIRi | A46129 |
RefSeqi | NP_776590.1, NM_174165.2 |
Genome annotation databases
Ensembli | ENSBTAT00000035069; ENSBTAP00000034949; ENSBTAG00000025088 |
GeneIDi | 281447 |
KEGGi | bta:281447 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95858 mRNA Translation: AAB59256.1 X63322 mRNA Translation: CAA44928.1 |
PIRi | A46129 |
RefSeqi | NP_776590.1, NM_174165.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IKU | NMR | - | A | 2-202 | [»] | |
1JSA | NMR | - | A | 2-202 | [»] | |
1LA3 | NMR | - | A | 2-202 | [»] | |
1OMR | X-ray | 1.50 | A | 2-202 | [»] | |
1OMV | X-ray | 1.90 | A | 2-202 | [»] | |
1REC | X-ray | 1.90 | A | 2-202 | [»] | |
2HET | X-ray | 3.00 | A/B/C/D | 2-190 | [»] | |
2I94 | NMR | - | A | 1-202 | [»] | |
4M2O | X-ray | 1.50 | A | 2-197 | [»] | |
4M2P | X-ray | 1.45 | A | 2-202 | [»] | |
4M2Q | X-ray | 1.90 | A | 2-202 | [»] | |
4MLW | X-ray | 1.45 | A | 2-202 | [»] | |
4YI8 | X-ray | 1.20 | A | 2-202 | [»] | |
4YI9 | X-ray | 1.35 | A | 2-202 | [»] | |
BMRBi | P21457 | |||||
SMRi | P21457 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ELMi | P21457 |
IntActi | P21457, 4 interactors |
MINTi | P21457 |
STRINGi | 9913.ENSBTAP00000034949 |
PTM databases
iPTMneti | P21457 |
Proteomic databases
PaxDbi | P21457 |
Genome annotation databases
Ensembli | ENSBTAT00000035069; ENSBTAP00000034949; ENSBTAG00000025088 |
GeneIDi | 281447 |
KEGGi | bta:281447 |
Organism-specific databases
CTDi | 5957 |
VEuPathDBi | HostDB:ENSBTAG00000025088 |
VGNCi | VGNC:33838, RCVRN |
Phylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000159441 |
HOGENOMi | CLU_072366_1_0_1 |
InParanoidi | P21457 |
OMAi | TQKLEWA |
OrthoDBi | 1271942at2759 |
TreeFami | TF300009 |
Miscellaneous databases
EvolutionaryTracei | P21457 |
Gene expression databases
Bgeei | ENSBTAG00000025088, Expressed in retina and 44 other tissues |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | RECO_BOVIN | |
Accessioni | P21457Primary (citable) accession number: P21457 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | September 29, 2021 | |
This is version 173 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families