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Protein

Multidrug resistance protein 1A

Gene

Abcb1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.1 Publication

Miscellaneous

In mouse the MDR gene family includes three or more related but distinct cellular genes.

Catalytic activityi

ATP + H2O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi423 – 430ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1066 – 1073ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTranslocase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 1A (EC:7.6.2.2)
Alternative name(s):
ATP-binding cassette sub-family B member 1A
MDR1A
Multidrug resistance protein 3
P-glycoprotein 3
Gene namesi
Name:Abcb1a
Synonyms:Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97570 Abcb1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicBy similarityAdd BLAST43
Transmembranei44 – 66HelicalAdd BLAST23
Topological domaini67 – 112ExtracellularBy similarityAdd BLAST46
Transmembranei113 – 133HelicalAdd BLAST21
Topological domaini134 – 182CytoplasmicBy similarityAdd BLAST49
Transmembranei183 – 204HelicalAdd BLAST22
Topological domaini205 – 211ExtracellularBy similarity7
Transmembranei212 – 232HelicalAdd BLAST21
Topological domaini233 – 290CytoplasmicBy similarityAdd BLAST58
Transmembranei291 – 312HelicalAdd BLAST22
Topological domaini313 – 326ExtracellularBy similarityAdd BLAST14
Transmembranei327 – 348HelicalAdd BLAST22
Topological domaini349 – 707CytoplasmicBy similarityAdd BLAST359
Transmembranei708 – 728HelicalAdd BLAST21
Topological domaini729 – 752ExtracellularBy similarityAdd BLAST24
Transmembranei753 – 773HelicalAdd BLAST21
Topological domaini774 – 828CytoplasmicBy similarityAdd BLAST55
Transmembranei829 – 849HelicalAdd BLAST21
Topological domaini850ExtracellularBy similarity1
Transmembranei851 – 870HelicalAdd BLAST20
Topological domaini871 – 930CytoplasmicBy similarityAdd BLAST60
Transmembranei931 – 953HelicalAdd BLAST23
Topological domaini954 – 969ExtracellularBy similarityAdd BLAST16
Transmembranei970 – 991HelicalAdd BLAST22
Topological domaini992 – 1276CytoplasmicBy similarityAdd BLAST285

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2573

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933361 – 1276Multidrug resistance protein 1AAdd BLAST1276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi87N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei656PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP21447
PaxDbiP21447
PeptideAtlasiP21447
PRIDEiP21447

PTM databases

iPTMnetiP21447
PhosphoSitePlusiP21447

Expressioni

Gene expression databases

BgeeiENSMUSG00000040584 Expressed in 215 organ(s), highest expression level in brain blood vessel
GenevisibleiP21447 MM

Interactioni

Subunit structurei

Interacts with PSMB5.By similarity

Protein-protein interaction databases

IntActiP21447, 1 interactor
MINTiP21447
STRINGi10090.ENSMUSP00000041204

Chemistry databases

BindingDBiP21447

Structurei

Secondary structure

11276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21447
SMRiP21447
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21447

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 353ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST304
Domaini388 – 624ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini707 – 996ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST290
Domaini1031 – 1269ABC transporter 2PROSITE-ProRule annotationAdd BLAST239

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00530000062896
HOVERGENiHBG080809
InParanoidiP21447
KOiK05658
OMAiQAVLFAK
OrthoDBiEOG091G0HVA
PhylomeDBiP21447
TreeFamiTF105193

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR039421 Type_I_exporter
PANTHERiPTHR24221 PTHR24221, 1 hit
PfamiView protein in Pfam
PF00664 ABC_membrane, 2 hits
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
SSF90123 SSF90123, 2 hits
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 2 hits
PS00211 ABC_TRANSPORTER_1, 2 hits
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequencei

Sequence statusi: Complete.

P21447-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELEEDLKGR ADKNFSKMGK KSKKEKKEKK PAVSVLTMFR YAGWLDRLYM
60 70 80 90 100
LVGTLAAIIH GVALPLMMLI FGDMTDSFAS VGNVSKNSTN MSEADKRAMF
110 120 130 140 150
AKLEEEMTTY AYYYTGIGAG VLIVAYIQVS FWCLAAGRQI HKIRQKFFHA
160 170 180 190 200
IMNQEIGWFD VHDVGELNTR LTDDVSKINE GIGDKIGMFF QAMATFFGGF
210 220 230 240 250
IIGFTRGWKL TLVILAISPV LGLSAGIWAK ILSSFTDKEL HAYAKAGAVA
260 270 280 290 300
EEVLAAIRTV IAFGGQKKEL ERYNNNLEEA KRLGIKKAIT ANISMGAAFL
310 320 330 340 350
LIYASYALAF WYGTSLVISK EYSIGQVLTV FFSVLIGAFS VGQASPNIEA
360 370 380 390 400
FANARGAAYE VFKIIDNKPS IDSFSKSGHK PDNIQGNLEF KNIHFSYPSR
410 420 430 440 450
KEVQILKGLN LKVKSGQTVA LVGNSGCGKS TTVQLMQRLY DPLDGMVSID
460 470 480 490 500
GQDIRTINVR YLREIIGVVS QEPVLFATTI AENIRYGRED VTMDEIEKAV
510 520 530 540 550
KEANAYDFIM KLPHQFDTLV GERGAQLSGG QKQRIAIARA LVRNPKILLL
560 570 580 590 600
DEATSALDTE SEAVVQAALD KAREGRTTIV IAHRLSTVRN ADVIAGFDGG
610 620 630 640 650
VIVEQGNHDE LMREKGIYFK LVMTQTAGNE IELGNEACKS KDEIDNLDMS
660 670 680 690 700
SKDSGSSLIR RRSTRKSICG PHDQDRKLST KEALDEDVPP ASFWRILKLN
710 720 730 740 750
STEWPYFVVG IFCAIINGGL QPAFSVIFSK VVGVFTNGGP PETQRQNSNL
760 770 780 790 800
FSLLFLILGI ISFITFFLQG FTFGKAGEIL TKRLRYMVFK SMLRQDVSWF
810 820 830 840 850
DDPKNTTGAL TTRLANDAAQ VKGATGSRLA VIFQNIANLG TGIIISLIYG
860 870 880 890 900
WQLTLLLLAI VPIIAIAGVV EMKMLSGQAL KDKKELEGSG KIATEAIENF
910 920 930 940 950
RTVVSLTREQ KFETMYAQSL QIPYRNAMKK AHVFGITFSF TQAMMYFSYA
960 970 980 990 1000
ACFRFGAYLV TQQLMTFENV LLVFSAIVFG AMAVGQVSSF APDYAKATVS
1010 1020 1030 1040 1050
ASHIIRIIEK TPEIDSYSTQ GLKPNMLEGN VQFSGVVFNY PTRPSIPVLQ
1060 1070 1080 1090 1100
GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE RFYDPMAGSV FLDGKEIKQL
1110 1120 1130 1140 1150
NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVSYEE IVRAAKEANI
1160 1170 1180 1190 1200
HQFIDSLPDK YNTRVGDKGT QLSGGQKQRI AIARALVRQP HILLLDEATS
1210 1220 1230 1240 1250
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIQNGKVKEH
1260 1270
GTHQQLLAQK GIYFSMVSVQ AGAKRS
Length:1,276
Mass (Da):140,647
Last modified:June 16, 2009 - v3
Checksum:i542E101DF18DDC52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526 – 527QL → HV in AAA39514 (PubMed:1972547).Curated2
Sequence conflicti526 – 527QL → HV in AAA03243 (PubMed:2473069).Curated2
Sequence conflicti939S → F in AAA39517 (PubMed:1969610).Curated1
Sequence conflicti1036V → F in AAA39517 (PubMed:1969610).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30697 mRNA Translation: AAA39517.1
M33581 mRNA Translation: AAA39514.1
M33580 Genomic DNA Translation: AAA39518.1
AY864315 mRNA Translation: AAW56448.1
CH466600 Genomic DNA Translation: EDL14677.1
M24417 mRNA Translation: AAA03243.1
CCDSiCCDS19084.1
PIRiA34175 DVMS1A
A34786
RefSeqiNP_035206.2, NM_011076.2
XP_006503618.1, XM_006503555.3
XP_006503619.1, XM_006503556.3
UniGeneiMm.207354

Genome annotation databases

EnsembliENSMUST00000047753; ENSMUSP00000041204; ENSMUSG00000040584
GeneIDi18671
KEGGimmu:18671
UCSCiuc008wkl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30697 mRNA Translation: AAA39517.1
M33581 mRNA Translation: AAA39514.1
M33580 Genomic DNA Translation: AAA39518.1
AY864315 mRNA Translation: AAW56448.1
CH466600 Genomic DNA Translation: EDL14677.1
M24417 mRNA Translation: AAA03243.1
CCDSiCCDS19084.1
PIRiA34175 DVMS1A
A34786
RefSeqiNP_035206.2, NM_011076.2
XP_006503618.1, XM_006503555.3
XP_006503619.1, XM_006503556.3
UniGeneiMm.207354

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G5UX-ray3.80A/B1-1276[»]
3G60X-ray4.40A/B1-1276[»]
3G61X-ray4.35A/B1-1276[»]
4KSBX-ray3.80A1-1276[»]
4KSCX-ray4.00A1-1276[»]
4KSDX-ray4.10A1-1276[»]
4LSGX-ray3.80A/B1-1276[»]
4M1MX-ray3.80A/B1-1276[»]
4M2SX-ray4.40A/B1-1276[»]
4M2TX-ray4.35A/B1-1276[»]
4Q9HX-ray3.40A1-1276[»]
4Q9IX-ray3.78A1-1276[»]
4Q9JX-ray3.60A1-1276[»]
4Q9KX-ray3.80A1-1276[»]
4Q9LX-ray3.80A1-1276[»]
4XWKX-ray3.50A1-1276[»]
5KO2X-ray3.30A/B1-1276[»]
5KOYX-ray3.85A/B1-1276[»]
5KPDX-ray3.35A/B1-1276[»]
5KPIX-ray4.01A/B1-1276[»]
5KPJX-ray3.50A1-1276[»]
6GDIelectron microscopy7.90A1-1276[»]
ProteinModelPortaliP21447
SMRiP21447
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21447, 1 interactor
MINTiP21447
STRINGi10090.ENSMUSP00000041204

Chemistry databases

BindingDBiP21447
ChEMBLiCHEMBL2573

PTM databases

iPTMnetiP21447
PhosphoSitePlusiP21447

Proteomic databases

MaxQBiP21447
PaxDbiP21447
PeptideAtlasiP21447
PRIDEiP21447

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047753; ENSMUSP00000041204; ENSMUSG00000040584
GeneIDi18671
KEGGimmu:18671
UCSCiuc008wkl.1 mouse

Organism-specific databases

CTDi18671
MGIiMGI:97570 Abcb1a

Phylogenomic databases

eggNOGiKOG0055 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00530000062896
HOVERGENiHBG080809
InParanoidiP21447
KOiK05658
OMAiQAVLFAK
OrthoDBiEOG091G0HVA
PhylomeDBiP21447
TreeFamiTF105193

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport

Miscellaneous databases

EvolutionaryTraceiP21447
PROiPR:P21447
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040584 Expressed in 215 organ(s), highest expression level in brain blood vessel
GenevisibleiP21447 MM

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR039421 Type_I_exporter
PANTHERiPTHR24221 PTHR24221, 1 hit
PfamiView protein in Pfam
PF00664 ABC_membrane, 2 hits
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
SSF90123 SSF90123, 2 hits
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 2 hits
PS00211 ABC_TRANSPORTER_1, 2 hits
PS50893 ABC_TRANSPORTER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiMDR1A_MOUSE
AccessioniPrimary (citable) accession number: P21447
Secondary accession number(s): Q5I1Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 16, 2009
Last modified: November 7, 2018
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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