UniProtKB - P21405 (RDRP_SCPMV)
Protein
Replicase polyprotein P2AB
Gene
ORF2A-2B
Organism
Southern cowpea mosaic virus (SCPMV) (Southern bean mosaic virus (strain cowpea))
Status
Functioni
responsible for cleavages of polyprotein P2A and replicase polyprotein P2AB.
covalently attached to the 5' extremity of the genomic and subgenomic RNAs. It may serve as a primer for the replicase.
replicates the viral genome.PROSITE-ProRule annotation
Catalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 181 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 216 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 284 | For protease activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- serine-type endopeptidase activity Source: InterPro
GO - Biological processi
- RNA-protein covalent cross-linking Source: UniProtKB-KW
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Transferase |
Biological process | Viral RNA replication |
Ligand | Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein P2ABCleaved into the following 4 chains: Serine protease (EC:3.4.21.-) Alternative name(s): RdRp |
Gene namesi | ORF Names:ORF2A-2B |
Organismi | Southern cowpea mosaic virus (SCPMV) (Southern bean mosaic virus (strain cowpea)) |
Taxonomic identifieri | 196398 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Sobelivirales › Solemoviridae › Sobemovirus |
Virus hosti | Glycine max (Soybean) (Glycine hispida) [TaxID: 3847] Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885] Vigna mungo (Black gram) (Phaseolus mungo) [TaxID: 3915] Vigna unguiculata (Cowpea) [TaxID: 3917] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 7 – 27 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 37 – 57 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 77 – 94 | HelicalSequence analysisAdd BLAST | 18 |
GO - Cellular componenti
- host cell membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000222496 | 1 – 956 | Replicase polyprotein P2ABAdd BLAST | 956 | |
ChainiPRO_0000409843 | 1 – 132 | N-terminal proteinSequence analysisAdd BLAST | 132 | |
ChainiPRO_0000409844 | 133 – 325 | Serine proteaseSequence analysisAdd BLAST | 193 | |
ChainiPRO_0000409845 | 326 – 402 | VPgSequence analysisAdd BLAST | 77 | |
ChainiPRO_0000409846 | 403 – 956 | RNA-directed RNA polymeraseSequence analysisAdd BLAST | 554 |
Post-translational modificationi
The polyprotein is proteolytically cleaved into several chains by the viral protease.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 132 – 133 | Cleavage; by viral serine proteaseSequence analysis | 2 | |
Sitei | 325 – 326 | Cleavage; by viral serine proteaseBy similarity | 2 | |
Sitei | 402 – 403 | Cleavage; by viral serine proteaseSequence analysis | 2 |
Keywords - PTMi
Covalent protein-RNA linkageProteomic databases
PRIDEi | P21405 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 135 – 330 | Peptidase S39PROSITE-ProRule annotationAdd BLAST | 196 | |
Domaini | 694 – 808 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 115 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 380 – 383 | Poly-Asp | 4 |
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR000382, Peptidase_S39B_luteovirus IPR001795, RNA-dir_pol_luteovirus IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF02123, RdRP_4, 1 hit |
PRINTSi | PR00914, LVIRUSRNAPOL |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein P2AB (identifier: P21405-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYRPSCLSYV LLVANMWSFA VCANAFIYGS YDPSHNIPIV ALMTLCATGL
60 70 80 90 100
WLSTSVVSFG IRYVRVRVSP EKTQNRTIYV SSGLPHFDPV YGVVKKCEPM
110 120 130 140 150
GGGPAIELQV NPSWIHLLPT SPAINKVEVG QESAILGSTY SVVETGGEPK
160 170 180 190 200
SLVAVKSGDS TLGFGARVYH EGMDVLMVPH HVWYNDKPHT ALAKNGRSVD
210 220 230 240 250
TEDWEVEAAC ADPRIDFVLV KVPTAVWAKL AVRSTKVLAP VHGTAVQTFG
260 270 280 290 300
GQDSKQLFSG LGKAKALDNA WEFTHTAPTA KGWSGTPLYT RDGIVGMHTG
310 320 330 340 350
YVDIGTSNRA INMHFIMSCL VSKMETLPPE LGYREISLED VGLRSFEFLE
360 370 380 390 400
VEIENRGKVK LGKREFAWVP KGKAWADMLD DDDLPLPPKM VNGNLVWADA
410 420 430 440 450
QESFDGALPL NLLAGGRTQC LAAQIELGDY KFSCGPTHET GGMPFRNCGS
460 470 480 490 500
STCKFREVSR KPVADAVTAA TKVFPELSEL GWPERGSGAE IGSLLLQAGK
510 520 530 540 550
FVPTKAPSNL EQAYNNLLSR YPRSKPLACF RQGTWSFDAI FEQVVSKATS
560 570 580 590 600
AEINQKASPG VPLSRLATTN KDLMAQHMQF VAACVTGRVP LLASFEDIHA
610 620 630 640 650
LSPTEMVEMG LCDPVRLFVK QEPHPSRKLK EGRYRLISSV SIVDQLVERM
660 670 680 690 700
LFGAQNELEI AEWQSIPSKP GMGLSVIHQA DAIFRDLRVK HTVCPAAEAD
710 720 730 740 750
ISGFDWSVQD WELWADVEMR IVLGSFPPMM ARAARNRFSC FMNSVLQLSN
760 770 780 790 800
GQLLQQELPG IMKSGSYCTS STNSRIRCLM AELIGSPWCI AMGDDSVEGF
810 820 830 840 850
VEGAREKYAG LGHLCKDYKP CATTPTGQLY AVEFCSHVIK RNKAFLTSWP
860 870 880 890 900
KTLYRFLSTP RETLEDLERE LASSPMWHKI QSYVRSIPSP DKTARDKSIC
910 920 930 940 950
NGYPLDQEAI STSYSEYSSK SASAEATREA ACCAGAQAYP SWGIHGPYCS
GDHGEA
Note: Produced by -1 ribosomal frameshifting at the 2A-2B genes boundary.
Isoform Polyprotein P2A (identifier: Q83470-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q83470.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23021 Genomic RNA Translation: AAA46565.1 |
PIRi | B33739, RRBWSC |
RefSeqi | NP_042301.2, NC_001625.2 NP_042302.3, NC_001625.2 [P21405-1] |
Genome annotation databases
GeneIDi | 1481842 1481843 |
KEGGi | vg:1481842 vg:1481843 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23021 Genomic RNA Translation: AAA46565.1 |
PIRi | B33739, RRBWSC |
RefSeqi | NP_042301.2, NC_001625.2 NP_042302.3, NC_001625.2 [P21405-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PRIDEi | P21405 |
Genome annotation databases
GeneIDi | 1481842 1481843 |
KEGGi | vg:1481842 vg:1481843 |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR000382, Peptidase_S39B_luteovirus IPR001795, RNA-dir_pol_luteovirus IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF02123, RdRP_4, 1 hit |
PRINTSi | PR00914, LVIRUSRNAPOL |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RDRP_SCPMV | |
Accessioni | P21405Primary (citable) accession number: P21405 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | June 28, 2011 | |
Last modified: | December 2, 2020 | |
This is version 103 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |